The First Peoples Hospital Of Lianyungang

Xinpu, China

The First Peoples Hospital Of Lianyungang

Xinpu, China
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Sun Z.-X.,China Pharmaceutical University | Sun Z.-X.,The First Peoples Hospital of Lianyungang | Liu N.-F.,China Pharmaceutical University | Liu N.-F.,Nanjing Southeast University
Chinese Pharmaceutical Journal | Year: 2014

METHODS: To summarise on four aspects: the biomarker guided guideline-based drug therapy, the new evidence-based pharmacotherapeutics, the prevention of adverse reactions, and the clinical drug evaluation, based on the latest evidence-based clinical practice guidelines.RESULTS AND CONCLUSION: In order to provide clinical pharmacy services to meet the needs of clinic and patients, improve the prognosis and quality of life of patients, ensure medical safety and improve quality of medical care, clinical pharmacists should take the initiative to track the latest evidence-based clinical practice guidelines, follow and adhere guideline-based drug therapy strategy, and put the latest evidence-based pharmacotherapeutical achievement into practice.OBJECTIVE: To elaborate the promotion and guidance of clinical practice guidelines to good practice of evidence-based clinical pharmacy, and the promotion to scientifical and rational use of drugs. ©, 2014, Chinese Pharmaceutical Association. All right reserved.


Zuo L.,Nanjing Southeast University | Shi L.,The First Peoples Hospital of Lianyungang | Yan F.,Nanjing Southeast University
Neuroscience Letters | Year: 2016

Background: Sympathetic nervous system(SNS) is involved in the mechanism of immune suppression after stroke. Furthermore, as the pro-inflammatory effect of nuclear factor kappa B(NF-kB) is inhibited after stroke, which is regulated by cyclic adenosine monophosphate(cAMP) and proteinkinase A(PKA). The cAMP-PKA-NF-kB pathway might play an important role in noradrenergic-mediated immune dysfunction. Aim: The purpose of our research is to analyze how SNS interfere with the immune system after acute stroke and the underlying mechanism of cAMP-PKA-NF-kB pathway in regulating the inflammation. Methods: 32 healthy male Sprague-Dawley rats were divided into 4 groups equally and randomly (1) Sham operation group; (2) middle cerebral artery occlusion; (MCAO) control group; (3) propranolol MCAO group; (4) isopropylarterenol sham group. 72 h later after MCAO or sham operation, tumor necrosis factor-α(TNF-α)and interleukine-10(IL-10) in serum as well as cAMP, PKA and NF-kB in spleen cells were tested. Results: TNF-α decreased while IL-10 increased in serum after acute ischemia stroke (p < 0.05). Meanwhile, the levels of cAMP and PKA in spleen both increased in MCAO model while the expression of NF-kB was inhibited (p < 0.05). When propranolol was used to inhibit SNS, all of the results reversed (p < 0.05). But the reversed results were still significantly different from the sham operation group (p < 0.05). Isopropylarterenol administrated rats appeared the same trend as MCAO group when compared to the sham operation group (p < 0.05). However, the differences still existed (p < 0.05). Conclusion: On account of the SNS activation after stroke, epinephrine activates the expression of cAMP, which further increases the level of PKA. Therefore, the level of nuclear factor NF-kB is down-regulated. Since the pro-inflammatory effect of NF-kB slacked, the immune system may be inhibited after stroke. © 2016.


Zhang J.,The First Peoples Hospital Of Lianyungang | Liu J.,The First Peoples Hospital Of Lianyungang | He W.,The First Peoples Hospital Of Lianyungang | Wang M.,The First Peoples Hospital Of Lianyungang
International Journal of Clinical and Experimental Medicine | Year: 2017

Objective: This meta-analysis aimed to illustrate the efficacy and safety of oral tranexamic acid (TXA) for reducing blood lossafter total joint arthroplasty (TJA). Methods: PubMed, Embase, the Cochrane Central Register of Controlled Trials (CENTRAL), Web of Science and Google database were searched for comparative studies comparing oral TXA versus control group or intravenous TXA for patients prepared for TJA. The outcomes including need for transfusion, hemoglobin drop, length of hospital stay and drain volume. We calculated risk ratio (RR) with a 95% confidence interval (CI) for need for transfusion, and the weighted mean difference (WMD) with a 95% CI for hemoglobin drop, length of hospital stay and drain volume. A random-effects model was used for all comparisons and Stata 12.0 was used for meta-analysis. Results: Six clinical trials (4 RCTs and 2 non-RCTs) involving 3258 patients were finally included for this meta-analysis. When compared with control group, oral TXA was associated with less need for transfusion, hemoglobin drop, drain volume and length of hospital stay (P<0.05). When compared with IV TXA, oral TXA was associated with more hemoglobin drop (P<0.05). However, there was no significant difference between the need for transfusion, drain volume and the length of hospital stay between oral TXA with IV TXA. Conclusion: Oral TXA has comparable hemostasis effects with IV TXA, and may reduce the costs for patients prepared for TJA. However, the limited quality and number of the included studies, more high quality and multi center RCTs are still need to recommend for routine administration. © 2017, E-Century Publishing Corporation. All rights reserved.


Song H.,The First Peoples Hospital Of Lianyungang | Sun Z.,The First Peoples Hospital Of Lianyungang
3 Biotech | Year: 2017

Both hyperglycaemia and hyperlipidaemia are major risk factors for the development of coronary artery diseases and atherosclerosis, and therefore therapeutic drugs must be developed for treatment of them. Pumpkin polysaccharides (PPs) are biomacromolecules with varying bioactivities. In this study, PPs were extracted with commercial thermostable α-amylase, and their hypolipidaemic and hypoglycaemic activities were evaluated. Twenty four KKAy mice were divided into two groups: control was fed with high-fat diet; while the PP group was fed with high-fat diet with the addition of PPs at the same time, for 6 weeks. PP diet reduced body weight gain, the levels of plasma insulin, serum triglyceride, cholesterol, low-density lipoprotein cholesterol and blasting blood glucose in mice and improved the level of high-density lipoprotein cholesterol and liver glycogen. Results indicate that PPs had high hypolipidaemic and hypoglycaemic activities and could be used as potential drugs for treatment of hyperlipidaemia and hyperglycaemia. © 2017, Springer-Verlag GmbH Germany.


Yan W.,Soochow University of China | Yan W.,Taicang Center for Translational Bioinformatics | Yan W.,The 100th Hospital of PLA | Xu L.,Soochow University of China | And 7 more authors.
Oncotarget | Year: 2015

Acute myeloid leukemia (AML) in children is a complex and heterogeneous disease. The identification of reliable and stable molecular biomarkers for diagnosis, especially early diagnosis, remains a significant therapeutic challenge. Aberrant microRNA expression could be used for cancer diagnosis and treatment selection. Here, we describe a novel bioinformatics model for the prediction of microRNA biomarkers for the diagnosis of paediatric AML based on computational functional analysis of the microRNA regulatory network substructure. microRNA-196b, microRNA-155 and microRNA-25 were identified as putative diagnostic biomarkers for pediatric AML. Further systematic analysis confirmed the association of the predicted microRNAs with the leukemogenesis of AML. In vitro q-PCR experiments showed that microRNA-155 is significantly overexpressed in children with AML and microRNA-196b is significantly overexpressed in subgroups M4-M5 of the French-American-British classification system. These results suggest that microRNA-155 is a potential diagnostic biomarker for all subgroups of paediatric AML, whereas microRNA-196b is specific for subgroups M4-M5.


Sun S.,Fudan University | Liu N.-H.,The First Peoples Hospital Of Lianyungang | Huang S.-Q.,Fudan University
Journal of Clinical Monitoring and Computing | Year: 2015

To investigate the role of cerebral oxygen saturation (ScO2) for prediction of hypotension after spinal anesthesia for caesarean section. Forty-five parturients undergoing elective caesarean section under spinal anesthesia were selected. Blood pressure, heart rate and pulse oxygen saturation before and after anesthesia were recorded, and the association between changes in ScO2 before and after anesthesia with hypotension after spinal anesthesia was explored. Hypotension occurred in 32 parturients after spinal anesthesia. The decrease in ScO2 after spinal anesthesia in parturients with hypotension was larger than in parturients without hypotension (P < 0.05). The duration from the intrathecal injection to 5 % decrease in ScO2 was shorter than that from the intrathecal injection to the occurrence of hypotension (P < 0.05). The mean time from 5 % decrease in ScO2 to hypotension was 38 s. The area under the receiver operation characteristic curve was 0.83 for decrease in ScO2 for prediction of hypotension (P < 0.05), and the optimal threshold value was 4.5 %. The sensitivity, specificity, positive predictive value and negative predictive value of 4.5 % decrease in ScO2 for prediction of hypotension were 0.75, 0.78, 0.92 and 0.47, respectively. The decrease in ScO2 after spinal anesthesia is associated with hypotension after spinal anesthesia for cesarean section, and may be a clinically useful predictor. © 2015 Springer Science+Business Media New York


Luan H.-F.,The First Peoples Hospital of Lianyungang | Zhao Z.-B.,The First Peoples Hospital of Lianyungang | Zhao Q.-H.,Bengbu Medical College | Zhu P.,The First Peoples Hospital of Lianyungang | And 2 more authors.
Brazilian Journal of Medical and Biological Research | Year: 2012

The JAK2/STAT3 signal pathway is an important component of survivor activating factor enhancement (SAFE) pathway. The objective of the present study was to determine whether the JAK2/STAT3 signaling pathway participates in hydrogen sulfide (H2S) postconditioning, protecting isolated rat hearts from ischemic-reperfusion injury. Male Sprague-Dawley rats (230-270 g) were divided into 6 groups (N = 14 per group): time-matched perfusion (Sham) group, ischemia/reperfusion (I/R) group, NaHS postconditioning group, NaHS with AG-490 group, AG-490 (5 μM) group, and dimethyl sulfoxide (DMSO; <0.2%) group. Langendorff-perfused rat hearts, with the exception of the Sham group, were subjected to 30 min of ischemia followed by 90 min of reperfusion after 20 min of equilibrium. Heart rate, left ventricular developed pressure (LVDP), left ventricular end-diastolic pressure (LVEDP), and the maximum rate of increase or decrease of left ventricular pressure (± dp/dtmax) were recorded. Infarct size was determined using triphenyltetrazolium chloride (TTC) staining. Myocardial TUNEL staining was used as the in situ cell death detection method and the percentage of TUNEL-positive nuclei to all nuclei counted was used as the apoptotic index. The expression of STAT3, bcl-2 and bax was determined by Western blotting. After reperfusion, compared to the I/R group, H2S significantly improved functional recovery and decreased infarct size (23.3 ± 3.8 vs 41.2 ± 4.7%, P < 0.05) and apoptotic index (22.1 ± 3.6 vs 43.0 ± 4.8%, P < 0.05). However, H2S-mediated protection was abolished by AG-490, the JAK2 inhibitor. In conclusion, H2S postconditioning effectively protects isolated I/R rat hearts via activation of the JAK2/STAT3 signaling pathway.


PubMed | The Affiliated Huaian Hospital of Xuzhou Medical University, Jiangsu Huaian Maternity and Children Hospital, the Fourth Peoples Hospital of Huaian, Nanjing Medical University and 2 more.
Type: | Journal: Molecular therapy. Nucleic acids | Year: 2017

Owing to its easy-to-use and multiplexing nature, the genome editing tool CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats (CRISPR) associated nuclease 9) is revolutionizing many areas of medical research and one of the most amazing areas is its gene therapy potentials. Previous explorations into the therapeutic potentials of CRISPR-Cas9 were mainly conducted in vitro or in animal germlines, the translatability of which, however, is either limited (to tissues with adult stem cells amenable to culture and manipulation) or currently impermissible (due to ethic concerns). Recently, important progresses have been made on this regard. Several studies have demonstrated the ability of CRISPR-Cas9 for in vivo gene therapy in adult rodent models of human genetic diseases delivered by methods that are potentially translatable to human use. Although these recent advances represent a significant step forward to the eventual application of CRISPR-Cas9 to the clinic, there are still many hurdles to overcome, such as the off-target effects of CRISPR-Cas9, efficacy of homology-directed repair, fitness of edited cells, immunogenicity of therapeutic CRISPR-Cas9 components, as well as efficiency, specificity, and translatability of in vivo delivery methods. In this article, we introduce the mechanisms and merits of CRISPR-Cas9 in genome editing, briefly retrospect the applications of CRISPR-Cas9 in gene therapy explorations and highlight recent advances, later we discuss in detail the challenges lying ahead in the way of its translatability, propose possible solutions, and future research directions.


PubMed | Jiangsu University, The First Peoples Hospital of Lianyungang and Ningbo Medical Center Lihuili Eastern Hosipital
Type: | Journal: Minimally invasive therapy & allied technologies : MITAT : official journal of the Society for Minimally Invasive Therapy | Year: 2017

The aim of this study is to assess the long-term clinical and radiological outcomes between minimally invasive (MIS) and conventional transforaminal lumbar interbody fusion (TLIF) in treating one-segment lumbar disc herniation (LDH).One-hundred and six patients treated by MIS-TLIF (50 cases) or conventional TLIF (56 cases) were included. Perioperative results were evaluated. Clinical outcomes were compared preoperatively and postoperatively. Radiologic parameters were based on a comparison of preoperative and three-year postoperative lumbar lordosis, segmental lordosis, sacral slope, the cross-sectional area of the paraspinal muscle and fusion rates.MIS TILF had significantly less blood, shorter operation time, mean return to work time and lower intramuscular pressure compared with the conventional group during the operation. VAS scores for lower back pain and ODI in MIS-TLIF were significantly decreased. The mean cross-sectional area of the paraspinal muscle was significantly decreased after surgery in the conventional TLIF group and no significant intragroup differences were established in the MIS-TLIF group. No significant differences were found in fusion rate, lumbar lordosis, segmental lordosis and sacral slope.Both MIS and conventional TLIF were beneficial for patients with LDH. However, MIS-TLIF manifests a great improvement in perioperative outcomes, low back pain, disability and preventing paraspinal muscle atrophy during the follow-up period observation.


Zhang L.,Jiangsu University | Zhang L.,the First Peoples Hospital of Lianyungang
Oncogene | Year: 2014

SALL4, a zinc-finger transcriptional factor for embryonic stem cell self-renewal and pluripotency, has been suggested to be involved in tumorigenesis. The role of SALL4 in human gastric cancer, however, remains largely unknown. In this study, we demonstrated that SALL4 was aberrantly expressed at both mRNA and protein levels in human gastric cancer tissues, and SALL4 level was highly correlated with lymph node metastasis. Enforced expression of SALL4 enhanced the proliferation and migration of human gastric cancer cells, whereas knockdown of SALL4 by siRNA led to the opposite effects. In addition, SALL4 overexpression promoted the growth and metastasis of gastric xenograft tumor in vivo. SALL4 overexpression induced epithelial-mesenchymal transition (EMT) in gastric cancer cells, with increased expression of Twist1, N-cadherin and decreased expression of E-cadherin. Moreover, SALL4 promoted the acquirement of stemness in gastric cancer cells through the induction of Bmi-1 and Lin28B. Taken together, our findings indicate that SALL4 has oncogenic roles in gastric cancer through the modulation of EMT and cell stemness, suggesting SALL4 as a novel target for human gastric cancer diagnosis and therapy.

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