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PubMed | The First Peoples Hospital of Jining City, Tianjin Anning Hospital, Central South University, Jining Medical University and Tianjin Anding Hospital
Type: | Journal: Neuroscience letters | Year: 2016

Psychological stress affects human health, and chronic stress leads to life-threatening diseases, such as depression and post-traumatic stress disorder. Psychological stress coping mechanisms involve the brain-derived neurotrophic factor (BDNF) and downstream cAMP response element binding protein (CREB), which are targets of the adverse effects of stress paradigms. Fourty-seven adult male Sprague-Dawley rats were divided into control, physical stress and six psychological stress groups which were assayed at 0h, 0.5h, 1h, 2h, 6h and 24h after communication box (CB) stress induction. Behavioral assessment using open field and elevated plus maze tests determined that CB stress significantly increased anxiety. After CB stress, the alternation of mRNA levels of BDNF and CREB were assessed at different time points by in situ hybridization. The mRNA levels of BDNF and CREB were significantly decreased, then gradually recovered over 24h to maximum levels in the hippocampus (CA1 region), prefrontal cortex (PFC), central amygdaloid nuclei (AG), shell of accumbens nucleus (NAC), periaqueductal gray (PAG) and ventral tegmental area, except for the ventral tegmental area (VTA). Moreover, mRNA levels of BDNF and CREB were positively correlated in all examined brain regions, except for the VTA region at 0 and 24h after CB stress induction. These findings suggest that BDNF and CREB may belong to the same pathway and be involved in psychological stress response mechanisms, and protect the organism from stress induced, aversive processes leading to disease.


PubMed | the First Peoples Hospital of Jining City, Binzhou Medical University and Jining Medical University
Type: | Journal: Scientific reports | Year: 2015

Drug-associated contextual cues contribute to drug craving and relapse after abstinence, which is a major challenge to drug addiction treatment. Previous studies showed that disrupting memory reconsolidation impairs drug reward memory. However, the underlying mechanisms remain elusive. Although actin polymerization is involved in memory formation, its role in the reconsolidation of drug reward memory is unknown. In addition, the specific brain areas responsible for drug memory have not been fully identified. In the present study, we found that inhibiting actin polymerization in the nucleus accumbens (NAc) shell, but not the NAc core, abolishes morphine-induced conditioned place preference (CPP) by disrupting its reconsolidation in rats. Moreover, this effect persists for more than 2 weeks by a single injection of the actin polymerization inhibitor, which is not reversed by a morphine-priming injection. Furthermore, the application of actin polymerization inhibitor outside the reconsolidation window has no effect on morphine-associated contextual memory. Taken together, our findings first demonstrate that inhibiting actin polymerization erases morphine-induced CPP by disrupting its reconsolidation. Our study suggests that inhibition of actin polymerization during drug memory reconsolidation may be a potential approach to prevent drug relapse.


PubMed | Biomedical Computation Center, The First Peoples Hospital of Jining City and Jiaxiang County Peoples Hospital
Type: | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2016

Metastatic melanoma is a rapidly progressing disease with high mortality rate and limited treatment options. Immunotherapy based on tumor-targeting cytotoxic T cell responses represents a promising strategy. To assist in its development, we examined the possibility and efficacy of using CD4


PubMed | The First Peoples Hospital of Jining City, Qingdao Municipal Hospital and Qingdao University
Type: Journal Article | Journal: The heart surgery forum | Year: 2015

The objective of this study was to evaluate the safety and efficacy of low-intensity warfarin treatment in Chinese patients after mitral valve replacement as well as the rationality of the INR standards of the hospital (The First Peoples Hospital of Jining City).We retrospectively analyzed 270 cases in our hospital from January 2009 to December 2013; 239 patients completed the 5-year follow-up. There were 192 male and 78 female subjects, age 32-65 years old with a mean age 51.51 12.12 years old. All eligible candidates received a Carbomedics artificial mechanical valve replacement and were anticoagulated under this hospitals current guidelines for postoperative anticoagulant therapy mitral valve replacement (INR 1.8-2.5). We analyzed the patients regularly recorded prothrombin times (PTs) and the occurrence of anticoagulation-related complications, such as bleeding, thrombosis, and embolism.A follow-up for 239 of 300 patients was performed for 3-60 months. Within this group, one patient died from cerebral hemorrhage, there was 1 case of subcutaneous bleeding, 1 case of epistaxis, 12 cases of gingival bleeding, in 15 menstrual quantity increased, and in 1 case we found cerebral infarction.In conclusion, low-intensity anticoagulation after mitral Carbomedics valve replacement is therapeutically effective and safe. There is room to improve the anticoagulation standard currently used, perhaps at a slightly lower level than this standard suggests. From our research we can formulate individualized treatment plans and effectively reduce the occurrence of complications.


Sun L.-J.,The Affiliated Hospital of Jining Medical College | Zhang L.-H.,The First Peoples Hospital of Jining City | Wang L.-S.,The Affiliated Hospital of Jining Medical College
Journal of Practical Oncology | Year: 2015

Objective: To review the CT and MRI findings of adrenal adenoma containing ectocytic fat. Methods: Seven patients with adrenal adenoma containing ectocytic fat underwent non-enhanced and enhanced CT scan and 4 of them also underwent non-enhanced MRI examination. Clinical data and imaging findings of 7 patients were retrospectively reviewed. Results: Seven cases all had dominant non-fat components. Fatty foci were multiple (3~8) with diameters of 1.4~7.2 mm. CT values of fatty foci were -110~-46 HU, while CT values of non-fat components were 5.8~13.4 HU. There were calcifications near fatty foci in 5 cases. All cases enhanced heterogeneously. Greatly-enhanced nodules near fatty foci were detected in 4 cases, tumor arteries and veins were dilated in one case. Fatty foci were hypointense on fat-suppressed T1WI while hyperintense on non-fat-suppressed T1WI. The non-fat components of 4 cases showed high signal on the in-phase T1WI with signal dropout on the out-of-phase T1WI. Conclusion: Adrenal adenoma containing ectocytic fat has some characteristics on CT and MRI scanning. Imaging features of non-fat components are helpful for qualitative and differential diagnosis of the tumor. © 2015, Journal of Practical Oncology, Editorial Board. All right reserved.


Liu Z.-Y.,The First Peoples Hospital Of Jining City | Zhang C.,The First Peoples Hospital Of Jining City | Zhang L.,Shandong University
World Chinese Journal of Digestology | Year: 2016

AIM: To detect the expression of somatostafin receptor subtype 2 (SSTR2) in human colorectal neuroendocrine neoplasms and analyze its clinicopathological significance. METHODS: Clinical samples and clinicopathological information of patients with colorectal neuroendocrine neoplasms were collected. The expression of SSTR2 in tumor tissues was examined by immunohistochemical EnVision method. RESULTS: A total of 56 patients were enrolled in this study. The rate of positive expression of SSTR2 in colorectal neuroendocrine neoplasms was 76.8%. Positive expression of SSTR2 in tumor tissues was associated with lymph node metastasis, muscularis invasion and distant metastasis (P < 0.05), but not with sex or age (P > 0.05). In addition, a significant correlation was found between SSTR2 expression and tumor diameter and histological grade (P < 0.05). CONCLUSION: Abnormal expression of SSTR2 correlates closely with some important clinicopathological characteristics of colorectal neuroendocrine neoplasms. SSTR2 may be used as a new biomarker for the diagnosis and prognosis evaluation of colorectal neuroendocrine neoplasms. © 2016 Baishideng Publishing Group Inc. All rights reserved.


PubMed | the First Peoples Hospital of Jining City
Type: Journal Article | Journal: Diagnostic cytopathology | Year: 2016

Plexiform angiomyxoid myofibroblastic tumor (PAMT) is a rare mesenchymal tumor of the stomach. To date, about 40 cases of PAMT have been reported in the literature. This tumor is not specific in clinical manifestations and microscopically characterized by a plexiform growth pattern. Here, we report the case of an 11-year-old male patient who was diagnosed with PAMT. He had a complaint of right epigastric discomfort with episodic pain, gastroscopy displayed a submucosal bulge at the pylorus, and CT showed a mass in the right abdomen with uneven, delayed enhancement, and a partial gastrectomy revealed a tumor at the pylorus. Histologically, the tumor was multinodular and rich in blood vessels with thin wall; the interstitium had abundant myxomatous stroma; the tumor cells were spindle-shaped, star-shaped, or oval. Immunohistochemically, the tumor cells were positive for Calponin, Caldesmon, and SMA, but negative for CD34, ALK, S-100, desmin, CD117, and Dog-1. This patient was followed up for 12 months, and recurrence or metastasis was not observed. Diagn. Cytopathol. 2017;45:55-58. 2016 Wiley Periodicals, Inc.


PubMed | The First Peoples Hospital of Jining City and Jining Medical University
Type: Journal Article | Journal: Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association | Year: 2016

To detect the skewness of TCR V and TCR V of patients with type 1 diabetes (T1D).The heparinized venous blood was collected from ten patients with T1D. The peripheral blood lymphocytes (PBL) were isolated and used to extract mRNA. Reverse amplyfication was performed for cDNA synthesis. The skewness of TCR V and V was detected with real-time florescence quantitative polymerase chain reaction (RQ-PCR) and analyzed by DNA melting-curve analysis technique, respectively.Among the TCR V genes, the skewness frequency rate (SFR) of V22 was 30%; both of V5 and V24 were 20%; V28 was the only restricted-clone gene with the SFR of 10%. In all the V genes, V7 and V17 were the the highest expression genes, and their SFRs were both 60%. V11 was near them with the SFR of 40%; the restricted clonal genes were V18 and V20, their SFRs were 10% and 20%, respectivley.There are skewd genes in TCR V and TCR V, which are probably relative to the onset of T1D.


PubMed | The First Peoples Hospital of Jining City and Shandong Cancer Hospital
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

The aim of this study was to explore epidermal growth factor receptor (EGFR) gene amplification and its relationship with cancer invasion and metastasis in non-small cell lung cancer (NSCLC). EGFR amplification in 45 patients with NSCLC and 15 subjects with normal lung tissues was detected by fluorescence in situ hybridization. The relationship between EGFR amplification and the clinicopathologic features of NSCLC was analyzed. EGFR gene amplifications were identified in 2 of 15 normal lung tissues (13.33%) and in 29 of 45 NSCLCs (64.44%). Patients <60 years had a 66.67% EGFR amplification rate, while patients 60 years had a rate of 62.50%. The EGFR amplification rates in male and female patients were 64.0% (16/25) and 65.0% (13/20), respectively. Pathologically, the EGFR amplification rate of patients with squamous cell carcinoma was 56.52% (13/23), and with adenocarcinoma was 72.72% (16/22). The EGFR amplification rate in NSCLCs with well-moderate differentiation was lower than in those with poor differentiation; 48.0% (12/25) vs 85.0% (17/20), respectively. Patients with lymph node metastasis had nearly double the amplification rate than those without metastasis; 90.0% (18/20) vs 44.0% (11/25), respectively. The rate of EGFR amplification was significantly higher in NSCLC than in normal lung tissue (64.44 vs 13.33%, P < 0.05), and was not correlated with age or gender (P > 0.05), but increased with clinical stage in NSCLCs (P < 0.05). Overall, these studies found that the rate of EGFR gene amplification was increased significantly in NSCLC and was closely related to lymphatic metastasis and TNM stage.


PubMed | The First Peoples Hospital of Jining City
Type: Journal Article | Journal: Journal of cancer research and therapeutics | Year: 2016

Diffusion.weighted imaging. (DWI) combined with its apparent diffusion coefficient. (ADC) value shows great significance in the differential diagnosis of human tumors. This meta.analysis is to determine whether ADC valued in DWI could contribute to the differential diagnosis of positive and negative lymph node. (LN) metastasis in cervical cancer. (CC) or not.A series of types of computerized databases were used searching for eligible studies relied on a strict inclusion and exclusion criteria. Two investigators were involved in the process of selecting articles and extracting dataset. Standardized mean differences (SMD) for the assessment of ADC values in positive and negative LN metastasis in CC patients were calculated.Fifteen cohort studies composed of 687 cases diagnosed with cervical tumor were incorporated into the current meta-analysis. Statistical analysis showed that the ADC value in positive LN metastasis was significantly lower than that with negative LN metastasis [SMD = 1.02, 95% confidence interval (CI) =0.54~1.50, P < 0.001]. Stratified by country, a lower ADC value in tumor tissues with LN metastasis was detected in comparison to that of tumor tissues without LN metastasis among China (SMD = 1.28, 95# CI = 0.62~1.94, P < 0.001) and Korea subgroups (SMD = 1.09, 95% CI = 0.65~1.52, P < 0.001).The ADC values in CC tissues with LN metastasis were significantly lower than those without LN metastasis, suggesting that DWI appears to improve diagnostic performance and can be a useful adjunct imaging for identifying LN metastasis in CC patients.

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