Gu Z.,The First Peoples Hospital Of Chengdu |
Qiu G.,Peking Union Medical College |
Zhang Y.,The First Peoples Hospital Of Chengdu
International Journal of Clinical and Experimental Medicine | Year: 2015
Objective: We explored the association between genetic polymorphisms of HAIRY-AND-ENHANCER-OF-SPLIT-7 (HES7) and congenital scoliosis (CS) in 246 cases of congenital scoliosis and non-congenital controls, in which the age and sex were fully matched. All participants were Chinese Han population. Methods: The genome DNA was extracted from peripheral blood sample. Two SNPs were defined for HES7 using NCBI database. The genotypes of two SNPs were determined by SNP stream UHT Genotyping System. Results: Polymorphisms were found in both SNPs and in accordance with Hardy-Weinberg equilibrium. For SNP rs3027279, the difference of two alleles (C and A) frequencies between CS and control groups Was statistically significant. Analysis also showed the difference of two genotypes (C/C and C/A) frequencies between two groups was significant (c2=5.857, P<0.05). For SNP rs1442849, both difference of two alleles (A and G) frequencies and difference of three genotypes (G/G, G/A and AA) frequencies between two groups were shown statistically significant. Conclusions: The unconditional Logistic regression analysis showed A/A genotype of SNP rsl442849 may be a protective factor (P=0.018<0.05, OR-0.35, 95% CI=0.17-0.74) for the onset of CS, while C/A genotype of SNP rs3027279 increased the onset risk (P=0.015<0.05, OR=1.93, 95% CI=1.13-3.30) of CS. Linkage disequilibrium analysis demonstrated the existence of linkage disequilibrium between the two SNPs. © 2015, E-Century Publishing Corporation. All rights reserved.
PubMed | Peoples Hospital of Tongchuan North Hospital, Xi'an Jiaotong University and the First Peoples Hospital of Chengdu
Type: Journal Article | Journal: Cell biology international | Year: 2016
The aim of this study is to investigate the effect of hepatitis B virus X (HBx) protein on the apoptosis of placental trophoblastic cells and its potential mechanism. A pcDNA3.1 expression vector of HBx gene was built and transfected into JEG-3 and HTR-8 human placental trophoblastic cell lines, respectively. After transfection for 48h, RT-PCR and immunofluorescence analyses showed that HBx mRNA and protein expression was detected in JEG-3 and HTR-8 cells. Flow cytometry revealed that early apoptosis of JEG-3 and HTR-8 cells was reduced by pcDNA-HBx transfection. Immunofluorescence and Western blotting showed that PI3K and p-Akt were significantly upregulated in HTR-8 cells. HBx ectopic expression did not change the viability of JEG-3 and HTR-8 cells when the PI3K/Akt pathway was blocked by its specific inhibitor LY294002. Moreover, the pcDNA-PI3K expression vector and pcDNA-HBx were transfected individually or co-transfected into the cells. The results showed that pcDNA-PI3K/pcDNA-HBx co-transfection promoted the expression of PI3K protein compared with the pcDNA-PI3K transfection group but did not increase the expression of HBx protein compared with pcDNA-HBx transfection group. In conclusion, HBx gene can be transferred into JEG-3 and HTR-8 human placental trophoblastic cell lines and cause inhibition of cell apoptosis. Its effect of apoptosis inhibition is related to the activation of the PI3K/Akt signaling pathway.
PubMed | The First Peoples Hospital of Chengdu and Peking Union Medical College
Type: | Journal: BioMed research international | Year: 2014
Idiopathic scoliosis is the most common pediatric spinal deformity affecting 1% to 3% of the population, and adolescent idiopathic scoliosis (AIS) accounts for approximately 80% of these cases; however, the etiology and pathogenesis of AIS are still uncertain. The current study aims to identify the relationship between calmodulin 1 (CALM1) gene and AIS predisposition, to identify the relationship between the genotypes of the SNPs and the clinical phenotypes of AIS.146 AIS patients and 146 healthy controls were enrolled into this case-control study. 12 single nucleotide polymorphisms (SNPs) candidates in CALM1 gene were selected to determine the relationship between CALM1 gene and AIS predisposition. Case-only study was performed to determine the effects of these variants on the severity of the condition.Three SNPs from 12 candidates were found to be associated with AIS predisposition. The ORs were observed as 0.549 (95% CI 0.3519-0.8579, P = 0.0079), 0.549 (95% CI 0.3519-0.8579, P = 0.0079), and 1.6139 (95% CI 1.0576-2.4634, P = 0.0257) for rs2300496, rs2300500, and rs3231718, respectively. There was no statistical difference between main curve, severity, and genotype distributions of all of 12 SNPs.Genetic variants of CALM1 gene are associated with AIS susceptibility.
Liu W.,Southern Medical University |
Song N.,The First Peoples Hospital Of Chengdu |
Yao H.,The First Peoples Hospital Of Chengdu |
Zhao L.,Southern Medical University |
And 2 more authors.
Medical Science Monitor | Year: 2015
Background: Although Helicobacter pylori infection is necessary for development of gastric adenocarcinoma (GAC), the underlying mechanism remains poorly defined. This study aimed to explore how miR-221 and miR-222 are dysregulated after H. pylori infection and how these 2 miRNAs are involved in pathological development of gastric cancer. Material/Methods: qRT-PCR analysis was performed to quantify miR-221 and miR-222 expression in patients with H. pylori – induced chronic gastritis, H. pylori-negative healthy controls, and in gastric cancer tissues and the corresponding adjacent normal tissues. Cell models were used to verify the expression profile. Dual luciferase assay was performed to verify putative binding between miR-221 or miR-222 and RECK. A loss-and-gain function study was performed to assess the miR-221/miR-222-RECK axis in gastric cancer cells. Results: H. pylori infection leads to significantly higher miR-221 and miR-222 expression. MiR-221 and miR-222 can bind the same sequence of RECK 3’UTR, thereby modulating its expression. Through simultaneous regulation over RECK, miR-221 and miR-222 can promote gastric cancer cell growth and invasion. Conclusions: The miR-221/miR-222-RECK axis might be an important path modulating H. pylori infection-related gastric cancer development. © Med Sci Monit, 2015.
PubMed | The First Peoples Hospital of Chengdu and Southern Medical University
Type: | Journal: Medical science monitor : international medical journal of experimental and clinical research | Year: 2015
Although Helicobacter pylori infection is necessary for development of gastric adenocarcinoma (GAC), the underlying mechanism remains poorly defined. This study aimed to explore how miR-221 and miR-222 are dysregulated after H. pylori infection and how these 2 miRNAs are involved in pathological development of gastric cancer.qRT-PCR analysis was performed to quantify miR-221 and miR-222 expression in patients with H. pylori - induced chronic gastritis, H. pylori-negative healthy controls, and in gastric cancer tissues and the corresponding adjacent normal tissues. Cell models were used to verify the expression profile. Dual luciferase assay was performed to verify putative binding between miR-221 or miR-222 and RECK. A loss-and-gain function study was performed to assess the miR-221/miR-222-RECK axis in gastric cancer cells.H. pylori infection leads to significantly higher miR-221 and miR-222 expression. MiR-221 and miR-222 can bind the same sequence of RECK 3UTR, thereby modulating its expression. Through simultaneous regulation over RECK, miR-221 and miR-222 can promote gastric cancer cell growth and invasion.The miR-221/miR-222-RECK axis might be an important path modulating H. pylori infection-related gastric cancer development.
Li X.,The First Peoples Hospital of Chengdu |
Du Y.,North Sichuan Medical College
Journal of Experimental and Clinical Medicine | Year: 2010
A large outbreak of novel influenza A (H1N1) virus (swine origin influenza virus) infection in Milwaukee, Wisconsin, USA, occurred in late April 2009. There were a few reports of patients with neurologic complications associated with H1N1 virus infection. We report a patient in China with neurologic complications associated with novel influenza A (H1N1) virus infection to alert clinicians to it. © 2010 Taiwan Medical University.
Fang L.X.,The First Peoples Hospital of Chengdu |
Ping F.,University of Sichuan |
Ping D.Y.,North Central College |
Hui B.G.,Chinese Academy of Sciences |
Yan Y.X.,North Central College
Vaccine | Year: 2010
The socioeconomic status of the patients is the important factor for post-exposure prophylaxis (PEP). However, few investigations were designed to study the correlation between the socioeconomic status and PEP. This study set out to determine the importance of socioeconomic status for PEP. All of the 11,670 at-risk populations of rabies in the public health centre of San Sheng County in Chengdu from January 2002 to December 2009 were reviewed retrospectively. We identified 11,350 patients on vaccination and 550 patients with rabies immunoglobulin. RIG was administered to 4.85% bite victims attending the rabies prevention clinics, while 61.36% had a category III exposure. The incidence of receiving RIG in the population of the high level of income (49.38%) was much higher than the groups of the medium level (8.08%) and the low level of income (1.46%) (P<0.05). The incidence of receiving RIG with above high school (23.08%) was much higher than the groups of the primary school (3.01%), the junior school (12.56%) and the illiteracy (2.08%) (P<0.05). In the logistic regression analysis by stepwise approach, the socioeconomic status was the most important factor for PEP (95% CI 1.20-2.04). Vaccination and immunoglobulin proved to be the most prominent two factors for PEP but whether receiving Vaccination and immunoglobulin treatment or not is determined by the socioeconomic status. So, the socioeconomic status was the most important factor for PEP. © 2010 Elsevier Ltd.
Li X.,The first peoples hospital of Chengdu |
Xiong Y.,The first peoples hospital of Chengdu |
Fan X.,The first peoples hospital of Chengdu |
Feng P.,The first peoples hospital of Chengdu |
And 2 more authors.
Medecine et Maladies Infectieuses | Year: 2012
Objective: The authors investigated the role of . femA regulating gene on methicillin-resistant . Staphylococcus aureus (MRSA). Methods: High-level MRSA, low-level MRSA, and methicillin-susceptible . Staphylococcus aureus (MSSA) were determined by agar diffusion methods. β-lactamase was then detected by nitrocefin and the presence of mecA was determined by PCR. Only β-lactamase-negative but mecA-positive isolates were included in further studies. The . femA gene and its 250. bp upstream sequence were amplified by PCR. Expression levels of . femA were determined by real-time fluorescent quantitative PCR. The 250. bp upstream sequence of . femA was labeled by BrightStar Psoralen-Biotin and was detected by electrophoretic mobility shift assay (EMSA). Results: The expression levels of . femA in the three different groups (MSSA, low-level MRSA, and high-level MRSA) ranged from 3.53. ×. 10-3% to 29.91%, 5.54. ×. 10-3% to 3.1. ×. 102%, and 13.88% to 5.50. ×. 104%, respectively. EMSA could detect the signal shift in 55 high-level MRSA isolates but not in four low-level MRSA and four MSSA strains. Conclusion: The expression levels of . femA in high-level MRSA (non-β-lactamase-producing) were higher than in low-level MRSA and MSSA. The . femA regulating gene probably lies in the 250. bp upstream sequence in MRSA. High-level expression of . femA seems to be essential for high-level MRSA. © 2008 .
Short-term glucose metabolism and gut hormone modulations after billroth ii gastrojejunostomy in nonobese gastric cancer patients with type 2 diabetes mellitus, impaired glucose tolerance and normal glucose tolerance
Zhang X.-J.,University of Sichuan |
Xiao Z.,The First Peoples Hospital of Chengdu |
Yu H.-L.,University of Sichuan |
Zhang X.-X.,University of Sichuan |
And 2 more authors.
Archives of Medical Research | Year: 2013
Background and Aims: Roux-en-Y gastric bypass (RYGB) is effective in controlling blood glucose in obese patients with type 2 diabetes (T2DM). The alterations of gut hormones involving in glucose metabolism may play an important role. Our aim was to explore the short-term effects of Billroth II gastrojejunostomy (a similar type of RYGB) on glucose metabolism and gut hormone modulations in nonobese patients with different levels of blood glucose tolerance. Methods: Twenty one nonobese gastric cancer patients with different levels of blood glucose tolerance were submitted to Billroth II gastrojejunostomy. Among them, seven had T2DM, seven with impaired glucose tolerance (IGT) and the other seven had normal glucose tolerance (NGT). Body weight, glucose parameters, responses of plasma glucagon-like peptide-1 (GLP-1), peptide YY (PYY) and gastric inhibitory polypeptide (GIP) to 75 g glucose were measured at baseline and 3 months after surgery. Results: Similar weight losses were observed in all groups. Blood glucose was reduced in T2DM and IGT patients. Fasting and 30-min plasma glucose were increased significantly in NGT. GLP-1 showed insignificant alterations in all groups. PYY was evaluated in T2DM and IGT but remained unchanged in the NGT group. Decreased fasting and AUC GIP were observed in patients with T2DM; however, fasting and 30-min GIP were increased in NGT patients. Conclusions: Billroth II gastrojejunostomy is effective in reducing blood glucose in nonobese patients with T2DM and IGT but could deteriorate early blood glucose in nonobese NGT in a 3-month time period. Variations of glucose and gut hormone changes in the three groups suggest a role of proximal intestine in the pathophysiology of T2DM. © 2013 IMSS.
PubMed | University of Sichuan and The First Peoples Hospital of Chengdu
Type: Clinical Trial | Journal: Archives of medical research | Year: 2015
We targeted to investigate the efficacy and the mechanisms of two gastric bypass surgeries, Roux-en-y Gastric Bypass (RYGB) and Billroth II gastrojejunostomy on managing obese patients with T2DM and nonobese T2DM patients, respectively.Seven nonobese T2DM patients with gastric cancer submitted to Billroth II gastrojejunostomy were compared with nine obese T2DM patients undergoing RYGB about their baseline characteristics, weight loss and glycemic control, 3 months and 2 years after surgery. Meanwhile, -cell function, glucagon-like peptide 1 (GLP-1), peptide YY (PYY) and gastric inhibitory polypeptide (GIP) levels were also investigated.Significant weight loss and improvement of glycemic control were observed in both groups and in the two follow-up periods. Reduction of body mass index was greater in obese patients with T2DM. The efficacy of Billroth II gastrojejunostomy on controlling blood glucose of nonobese T2DM was similar to that of RYGB on managing obese T2DM. Insulin levels and HOMA-IR were decreased in obese T2DM patients, whereas they remained unchanged in nonobese T2DM patients. Generally, levels of GLP-1 and PYY were increased, whereas GIP levels were decreased in both groups.Glycemic control efficacy of Billroth II gastrojejunostomy on managing nonobese T2DM is similar to that of RYGB on treating obese T2DM in the short- and mid-term. The underlying mechanisms of both surgeries may be related to weight loss and gut hormone modulations.