Kunhua W.,The First Peoples Hospital of Yunnan Province
African journal of traditional, complementary, and alternative medicines : AJTCAM / African Networks on Ethnomedicines | Year: 2012
Xylene has been generally used as a clearing and deparaffinizing agent in histology. Because of the potential toxic and flammable nature of xylene, its substitutes have been introduced into some laboratories. In this study, we introduced a novel, non-toxic xylene substitute (SBO), which was generated through a mixture of 86% of white oil No.2 and 14% of N-heptane. SBO had a high boiling point (188°C) and flash point (144°C) coupled with a scentless and decreased volatility. To compare the effectiveness of SBO and xylene in histology, a wide range of tissue samples from rats and human beings were processed in parallel in SBO and xylene, subjected to various staining procedures. Similar to the xylene-processed paraffin blocks, the SBO-processed counterparts were easy to section without any evidence of cell shrinkage. Assessment of the SBO-treated sections stained with hematoxylin-eosin revealed a good maintenance of cell morphology and structure, and a clear definition of the cytoplasm and the nucleus. Moreover, comparable good results were achieved between the SBO- and xylene-processed tissues in other histochemical and immunohistochemical stainings. Six-month clinical applications at one department of pathology supported the potentials of SBO as a xylene substitute. In conclusion, we suggest that SBO is a safe and efficient substitute of xylene and may probably replace xylene without losing valuable diagnostic information.
Ying X.,Sun Yat Sen University |
Li-ya Q.,The First Peoples Hospital of Yunnan Province |
Feng Z.,Sun Yat Sen University |
Yin W.,Sun Yat Sen University |
Ji-hong L.,Sun Yat Sen University
Biomedicine and Pharmacotherapy | Year: 2015
Aberrant activation of the Wnt/β-catenin signal pathway is frequently observed in various human cancers. Therefore, it was speculated that adenomatous polyposis coli 2 (APC2) could play important roles in activating the Wnt/β-catenin pathway. In this present study, miR-939 expression was markedly upregulated in ovarian cancer tissues and ovarian cancer cells. In functional assays, Overexpression of miR-939 promoted the proliferation and anchorage-independent growth of ovarian cancer cells, whereas inhibition of miR-939 inhibited this effect. Bioinformatics analysis further revealed APC2, a putative tumor suppressor as a potential target of miR-939. Result of luciferase reporter assays showed that miR-939 directly binds to the 3'-untranslated region (3'-UTR) of APC2 mRNA. Furthermore, we demonstrated that miR-939 could reduce the Wnt/β-catenin signal pathway by suppressing APC2 directly, resulting in increasing expression of CyclinD1, MYC and TCF. In functional assays, APC2-silenced in miR-939-in-transfected ES-2 cells have positive effect to promote cell proliferation, suggesting that direct APC2 downregulation is required for miR-939-induced ovarian cancer cell proliferation. In sum, our data provided compelling evidence that miR-939 functioned as a potential tumor promoter by regulating the Wnt/β-catenin signal pathway through direct suppression of APC2 expression and might sever as a potential therapeutic target for ovarian cancer patients. © 2015 Elsevier Masson SAS.
Li R.,Peking Union Medical College |
Mao J.,Peking Union Medical College |
Yu K.,Peking Union Medical College |
Wang L.,The First Peoples Hospital of Yunnan Province
Asia Pacific Journal of Clinical Nutrition | Year: 2015
Objectives: Medium chain triglycerides (MCTs) are of nutritional interest for their unique properties on ingestion, absorption and metabolism. However, dietary and enteral MCTs usage in hospitalized patients in China requires evaluation. Methods: A retrospective analysis was conducted on 46 hospitalized patients who were administered MCTs therapy between January, 2012 and December, 2013 in Peking Union Medical College Hospital. The clinical parameters of subjects were evaluated 2 weeks after intervention. All indications and outcomes of MCTs therapy underwent evidence-based evaluation. A survey was conducted on a random sample of 77 doctors to clarify the awareness and knowledge of MCTs therapy among clinicians. Results: Among 46 cases undergoing MCTs therapy, there were 21 with gastrointestinal dysfunction (with improvement in 15 cases), 15 with lymphatic anomalies (with improvement in 7 cases), 5 with dyslipidemia (with improvement in 3 cases), 4 with exocrine pancreatic insufficiency (with improvement in 2 cases) and 1 with epilepsy (without improvement). All indications were evidence -based. Although MCTs were being utilized in an increasing number of patients, the survey revealed poor knowledge of their physicochemical properties, medical indications and the therapeutic basis of their use among clinicians. Conclusions: MCTs therapy is of benefit in the management of gastrointestinal malabsorption, pancreatic exocrine insufficiency, intestinal lymphangiectasia and dyslipidemia. However, randomized controlled trials with adequate sample size and longer follow-up are required to evaluate further their efficacy, and more educational programs are needed to ensure clinical competence for MCT therapy.
Li L.,The First Peoples Hospital of Yunnan Province
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2012
To review the research progress of the osteogenic effect of strontium (Sr) and its application in the orthopaedics. The recent literature concerning the osteogenic effect of Sr and its application in orthopaedics at home and abroad was extensively reviewed, and the research and development were summarized. Both in vivo and in vitro studies showed that Sr could enhance bone formation and inhibit bone resorption. Clinically, Sr was applied for treatment of osteoporosis, composite biomaterials in tissue engineering, and treatment of bone tumors and bone metastases. Sr is one important combined element of alternative materials in bone tissue engineering, and can strengthen the mechanical and biological properties of the bone replacement material, so it has some development potential in bone tissue engineering.
Liu Y.,The First Peoples Hospital of Yunnan Province |
Yu T.,The First Peoples Hospital of Yunnan Province |
Wu X.,The First Peoples Hospital of Yunnan Province
Journal of Practical Oncology | Year: 2016
Objective: To evaluate the risk factors of parametrial involvement in early stage cervical cancer. Methods: The clinical data of 890 patients with IA2-IIA2 stage cervical cancer, who underwent extensive hysterectomy and pelvic lymph node dissection, were retrospectively analyzed. Univariate and multivariate analyses were conducted to determine the risk factors associated with parametrial metastasis in early cervical cancer. Results: Postoperative pathological biopsy suggested that the rate of parametrial metastasis was 6.0% (53/890). Univariate analysis showed that clinical stage, tumor size, serum SCC-Ag level, tumor invasion depth, vascular invasion, surgical margin status, lymph node metastasis, involvement of uterine body and involvement of the vagina were associated with parametrial involvement in early cervical cancer (P<0.05). Multivariate analysis suggested that tumor mesenchyme invasion depth, vascular invasion and lymph node metastasis were independent risk factors of parametrial metastasis in early stage cervical cancer (P<0.01). Conclusion: The depth of tumor invasion in cervix, vascular invasion and lymph node metastasis are risk factors of parametrial metastasis in early stage cervical cancer. © 2016, Editorial Board of Journal of Practical Oncology. All right reserved.