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Zhou F.,PLA Fourth Military Medical University | Huo J.,The First Hospital of Yulin | Liu Y.,PLA Fourth Military Medical University | Liu H.,PLA Fourth Military Medical University | And 3 more authors.
Journal of Steroid Biochemistry and Molecular Biology | Year: 2016

Endometrial cancer (EC) is one of the most common gynecological malignancies in the world. Associations between fasting glucose levels (greater than 5.6. mmol/L) and the risk of cancer fatality have been reported. However, the underlying link between glucose metabolic disease and EC remains unclear. In the present study, we explored the influence of elevated glucose levels on the WNT/β-catenin pathway in EC. Previous studies have suggested that elevated concentrations of glucose can drive the hexosamine biosynthesis pathway (HBP) flux, thereby enhancing the O-GlcNAc modification of proteins. Here, we cultured EC cell lines, AN3CA and HEC-1-B, with various concentrations of glucose. Results showed that when treated with high levels of glucose, both lines showed increased expression of β-catenin and O-GlcNAcylation levels; however, these effects could be abolished by the HBP inhibitors, Azaserine and 6-Diazo-5-oxo-l-norleucine, and be restored by glucosamine. Moreover the AN3CA and HEC-1-B cells that were cultured with or without PUGNAc, an inhibitor of the O-GlcNAcase, showed that PUGNAc increased β-catenin levels. The results suggest that elevated glucose levels increase β-catenin expression via the activation of the HBP in EC cells. Subcellular fractionation experiments showed that AN3CA cells had a higher expression of intranuclear β-catenin in high glucose medium. Furthermore, TOP/FOP-Flash and RT-PCR results showed that glucose-induced increased expression of β-catenin triggered the transcription of target genes. In conclusion, elevated glucose levels, via HBP, increase the O-GlcNAcylation level, thereby inducing the over expression of β-catenin and subsequent transcription of the target genes in EC cells. © 2016 Elsevier Ltd. Source


Tong A.,Peking Union Medical College | Jia A.,The First Hospital of Yulin | Yan S.,Peking Union Medical College | Zhang Y.,Peking Union Medical College | And 2 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2014

Ectopic cortisol-producing adrenocortical adenomas (CPA) are extremely rare, and only four cases have previously been reported so far but the tumors were not ultrastructurally studied. Presented in this paper is the fifth case with ectopic CPA which was extensively examined to gain deeper insights in terms of the histopathological features and steroidogenic enzyme profile of the tumor. A 53-year-old woman complained of accidental discovery of left renal mass. She had a 5-year history of hypertension, weight gain, moon face, thin skin and systemic edema. These symptoms completely relieved after the tumor removal. Two years later, the above symptoms recurred, and a recurrent tumor was revealed in left renal hilum. The tumor was removed completely with relief of her symptoms of Cushing's syndrome. Histologically and ultrastructurally, the tumor was composed of compact cells and clear cells, and the former was prominent, suggesting an active secretory function of the tumor. The adenoma tissue showed a strong immunostaining for Melan-A, 3beta-hydroxysteroid dehydrogenase (HSD3B2) and 17alpha-hydroxylase1 (CYP17A1). Expression pattern for 11beta-hydroxylase 1 (CYP11B1), 11beta-hydroxylase 2 (CYP11B2), CYP17 and HSD3B2 mRNA in ectopic CPA was similar to that in the adrenal CPA. In conclusion, in terms of histopathological characteristic and steroidogenic enzyme profile, ectopic CPA is similar to adrenal CPA, suggesting that they are of identical cell origin. Source


Gao L.-M.,Xingyuan The Fourth Hospital | Gao Y.,The First Hospital of Yulin
International Eye Science | Year: 2015

AIM: To observe the clinical effect of houttuynia cordata eyedrops combined with basic fibroblast growth factor (bFGF) eyedrops in treating dry eye after keratorefractive surgery. METHODS: Respectively, 117 patients (204 eyes) were randomly assigned to treatment group (61 patients, 113 eyes) andthe control group (56 patients, 91 eyes). In control group, the eyes were used bFGF eyedrops four times a day, one drop at a time, and the treatment group was used bFGF eyedrops and houttuynia cordata eyedrops. After 2 wk and 4wk of treatment, clinic symptom, Shirmer I test (SIt), break-up time of tear film (BUT) and fluorescence staining were recorded respectively. RESULTS: After 2wk and 4wk of observation, the clinic symptom of two groups was both improved. SIt, BUT and fluorescence staining scores in the treatment group were significantly better than those in control group (P<0.05). After 4wk, the total effective rate of treatment group was 98%, while that in the control group was 90%, there was significant difference between the two groups (P=0.011). CONCLUSION: Houttuynia cordata eyedrops can improve the effect of bFGF eyedrops in treating dry eye after keratorefractive surgery. ©, 2015, International Journal of Ophthalmology (c/o Editorial Office). All right reserved. Source


Ma X.,Chongqing Medical University | Eyler L.T.,University of California at San Diego | Eyler L.T.,Desert Pacific Mental Illness Research Education and Clinical Center | Hu X.,Chongqing Medical University | And 15 more authors.
Behavior Genetics | Year: 2016

Cortical surface area (CSA) has particular relevance for understanding development, behavior, and the connection between brain structure and function. Little is known about genetic and environmental determinants of CSA during development. We utilized bivariate twin methods to identify global and regionally specific genetic factors which influence CSA in a preliminary sample of typically-developing adolescents, with hypotheses based on findings in middle-aged adults. Similar to previous findings, we observed high heritability for total CSA. There was also significant evidence for genetic influences on regional CSA, particularly when these were not adjusted for total CSA, with highest heritability in frontal cortex and relatively fewer genetic contributions to medial temporal cortical structures. Adjustment for total CSA reduced regional CSA heritability dramatically, but a moderate influence of genetic factors remained in some regions. Both global and regionally-specific genetic factors influence regional CSA during adolescence. © 2015, Springer Science+Business Media New York. Source


He H.,The First Hospital of Yulin | Zhu Q.,The First Hospital of Yulin | Wang Q.,The First Hospital of Yulin
International Eye Science | Year: 2015

AIM: To analyze the common causes and treatments methods of high intraocular pressure (>21 mmHg) after trabeculectomy. METHODS: Twenty-four eyes of glaucoma patients with high intraocular pressure after trabeculectomy in our hospital from July 2010 to September 2014 were retrospectively studied. The common cause and processing methods were analyzed. RESULTS: The causes which lead to early postoperative high intraocular pressure included high filtering bleb scar in 11 eyes (46%), filtration obstruction in 4 eyes (17%), malignant glaucoma 3 eyes (12%), iris resection of malignant glaucoma unreasonable 2 eyes (8%), hyphema 2 eyes (8%), wrap cystic filtering bleb 2 eyes (8%), etc. After proper treatments, intraocular pressures of all patients were bellowed 21 mmHg. CONCLUSION: High intraocular pressure with surgery for glaucoma is caused by multiple factors, preoperative and intraoperative avoid as far as possible, postoperative early detection for symptomatic treatment is the key to successful operation. ©, 2015, International Journal of Ophthalmology (c/o Editorial Office). All right reserved. Source

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