PubMed | The First Hospital of Yulin, Xian No 1 Hospital, PLA Fourth Military Medical University and Xian Central Hospital
Type: | Journal: Archives of gynecology and obstetrics | Year: 2016
Interleukin-37 (IL-37) has been identified as a novel anti-inflammatory cytokine which is involved in tumor development. This study aimed to evaluate the expression of IL-37 in serum and determine its clinical significance in human epithelial ovarian cancer (EOC).Enzyme-linked immunosorbent assay (ELISA) was performed to examine the serum IL-37 levels in 76 patients with EOC and 76 healthy controls. The association of IL-37 levels with clinical factors and prognosis of EOC patients was analyzed. The diagnostic accuracy of serum IL-37 was performed by receiver operator characteristic (ROC) curve analysis.Serum IL-37 levels in patients with EOC (187.375.57pg/ml) were significantly higher than those in healthy controls (84.8928.92pg/ml, P<0.001). High serum IL-37 levels were significantly associated with FIGO stage (P<0.001), tumor size (P=0.002), lymph node metastasis (P=0.021), positive recurrence (P=0.047) and residual tumor size (P<0.001). The Kaplan-Meier survival analysis demonstrated that high serum IL-37 levels were significantly associated with poor overall survival and the progression-free survival (log-rank, P=0.026, and P=0.039, respectively). Univariate and multivariate analysis indicated that serum IL-37 levels (HR=3.007, 95% CI 2.125-4.842, P=0.008) were an independent prognostic factor for EOC patients. ROC curve analyses revealed an AUC (the areas under the ROC curve) of 0.881 (95% CI 0.829-0.945; P<0.001).High serum IL-37 levels are associated with an unfavorable prognosis of EOC patients. IL-37 may serve as a promising and useful prognostic biomarker for EOC.
Tong A.,Peking Union Medical College |
Jia A.,The First Hospital of Yulin |
Yan S.,Peking Union Medical College |
Zhang Y.,Peking Union Medical College |
And 2 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2014
Ectopic cortisol-producing adrenocortical adenomas (CPA) are extremely rare, and only four cases have previously been reported so far but the tumors were not ultrastructurally studied. Presented in this paper is the fifth case with ectopic CPA which was extensively examined to gain deeper insights in terms of the histopathological features and steroidogenic enzyme profile of the tumor. A 53-year-old woman complained of accidental discovery of left renal mass. She had a 5-year history of hypertension, weight gain, moon face, thin skin and systemic edema. These symptoms completely relieved after the tumor removal. Two years later, the above symptoms recurred, and a recurrent tumor was revealed in left renal hilum. The tumor was removed completely with relief of her symptoms of Cushing's syndrome. Histologically and ultrastructurally, the tumor was composed of compact cells and clear cells, and the former was prominent, suggesting an active secretory function of the tumor. The adenoma tissue showed a strong immunostaining for Melan-A, 3beta-hydroxysteroid dehydrogenase (HSD3B2) and 17alpha-hydroxylase1 (CYP17A1). Expression pattern for 11beta-hydroxylase 1 (CYP11B1), 11beta-hydroxylase 2 (CYP11B2), CYP17 and HSD3B2 mRNA in ectopic CPA was similar to that in the adrenal CPA. In conclusion, in terms of histopathological characteristic and steroidogenic enzyme profile, ectopic CPA is similar to adrenal CPA, suggesting that they are of identical cell origin.
PubMed | The first Hospital of Yulin and Xi'an Jiaotong University
Type: | Journal: Drug development and industrial pharmacy | Year: 2016
Extensive or long-time use of corticosteroids often causes many toxic side-effects. The ion exchange resins and the coating material, Eudragit, can be used in combination to form a new oral delivery system to deliver corticosteroids.The resin microcapsule (DRM) composed by Amberlite 717 and Eudragit S100 was used to target hydrocortisone (HC) to the colon in order to improve its treatment effect on ulcerative colitis (UC) and reduce its toxic side-effects.Hydrocortisone sodium succinate (HSS) was sequentially encapsulated in Amberlite 717 and Eudragit S100 to prepare the HSS-loaded resin microcapsule (HSS-DRM). The scanning electron microscopy (SEM) was employed to investigate the morphology and structure of HSS-DRM. The in vitro release and in vivo studies of pharmacokinetics and intestinal drug residues in rat were used to study the colon-targeting of HSS-DRM. The mouse induced by 2,4,6-trinitrobenzenesulfonic acid was used to study the treatment of HSS-DRM on experimental colitis.SEM study showed good morphology and structure of HSS-DRM. In the in vitro release study,>80% of HSS was released in the colon environment (pH 7.4). The in vivo studies showed good colon-targeting of HSS-DRM (TThe resin microcapsule system has good colon-targeting and can be used in the development of colon-targeting preparations.
Gao L.-M.,Xingyuan The Fourth Hospital |
Gao Y.,The First Hospital of Yulin
International Eye Science | Year: 2015
AIM: To observe the clinical effect of houttuynia cordata eyedrops combined with basic fibroblast growth factor (bFGF) eyedrops in treating dry eye after keratorefractive surgery. METHODS: Respectively, 117 patients (204 eyes) were randomly assigned to treatment group (61 patients, 113 eyes) andthe control group (56 patients, 91 eyes). In control group, the eyes were used bFGF eyedrops four times a day, one drop at a time, and the treatment group was used bFGF eyedrops and houttuynia cordata eyedrops. After 2 wk and 4wk of treatment, clinic symptom, Shirmer I test (SIt), break-up time of tear film (BUT) and fluorescence staining were recorded respectively. RESULTS: After 2wk and 4wk of observation, the clinic symptom of two groups was both improved. SIt, BUT and fluorescence staining scores in the treatment group were significantly better than those in control group (P<0.05). After 4wk, the total effective rate of treatment group was 98%, while that in the control group was 90%, there was significant difference between the two groups (P=0.011). CONCLUSION: Houttuynia cordata eyedrops can improve the effect of bFGF eyedrops in treating dry eye after keratorefractive surgery. ©, 2015, International Journal of Ophthalmology (c/o Editorial Office). All right reserved.
PubMed | East China University of Science and Technology, The First Hospital of Yulin, First Affiliated Hospital of Guangzhou Medical University and Guangzhou University
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2017
Influenza remains one of the major epidemic diseases worldwide, and rapid virus replication and collateral lung tissue damage caused by excessive pro-inflammatory host immune cell responses lead to high mortality rates. Thus, novel therapeutic agents that control influenza A virus (IAV) propagation and attenuate excessive pro-inflammatory responses are needed. Polysaccharide extract from
PubMed | Chongqing Medical and Pharmaceutical College, University of California at San Diego, The First Hospital of Yulin, Chongqing Medical University and University of Sichuan
Type: Journal Article | Journal: Behavior genetics | Year: 2016
Cortical surface area (CSA) has particular relevance for understanding development, behavior, and the connection between brain structure and function. Little is known about genetic and environmental determinants of CSA during development. We utilized bivariate twin methods to identify global and regionally specific genetic factors which influence CSA in a preliminary sample of typically-developing adolescents, with hypotheses based on findings in middle-aged adults. Similar to previous findings, we observed high heritability for total CSA. There was also significant evidence for genetic influences on regional CSA, particularly when these were not adjusted for total CSA, with highest heritability in frontal cortex and relatively fewer genetic contributions to medial temporal cortical structures. Adjustment for total CSA reduced regional CSA heritability dramatically, but a moderate influence of genetic factors remained in some regions. Both global and regionally-specific genetic factors influence regional CSA during adolescence.
Bai X.-B.,The First Hospital of Yulin |
Zhao Q.-P.,Health Science University
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Humeral fractures can be treated by using rotary self-locking intramedullary nail. The previous spin in self-locking intramedullary nail has some limitations in application, so it should be improved in its design so as to obtain better biomechanical effect. OBJECTIVE: To improve the design of the rotary self-locking intramedullary nail of the humerus, and to analyze its biomechanical characteristics and application effect, and to provide reference for the clinical treatment of humeral fractures.METHODS: (1) The design of the rotary self-locking intramedullary nail was improved and tested by mechanics test. (2) A total of 63 cases of humeral fractures were randomly divided into two groups: control group (31 cases) and experimental group (32 cases). Patients in the control group received conventional rotary self-locking intramedullary nail, and patients in the experimental group received improved nail. RESULTS AND CONCLUSION: Results of mechanical test concerning anti-axial compression, tensile, torsion and bending were obviously better after improvement than before improvement. No significant difference in range of motion was detected between the two groups (P > 0.05). Compared with that before treatment, the range of motion was significantly increased after treatment in both groups (P < 0.01 or P < 0.05). The range of motion was significantly higher in the experimental group than in the control group (P < 0.05). Simultaneously, the excellent and good rate was significantly higher in the experimental group than in the control group (P < 0.05). These datasuggest that the improved rotary self-locking intramedullary nail has better mechanical characteristics and can obtain better clinical effect. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
He H.,The First Hospital of Yulin |
Zhu Q.,The First Hospital of Yulin |
Wang Q.,The First Hospital of Yulin
International Eye Science | Year: 2015
AIM: To analyze the common causes and treatments methods of high intraocular pressure (>21 mmHg) after trabeculectomy. METHODS: Twenty-four eyes of glaucoma patients with high intraocular pressure after trabeculectomy in our hospital from July 2010 to September 2014 were retrospectively studied. The common cause and processing methods were analyzed. RESULTS: The causes which lead to early postoperative high intraocular pressure included high filtering bleb scar in 11 eyes (46%), filtration obstruction in 4 eyes (17%), malignant glaucoma 3 eyes (12%), iris resection of malignant glaucoma unreasonable 2 eyes (8%), hyphema 2 eyes (8%), wrap cystic filtering bleb 2 eyes (8%), etc. After proper treatments, intraocular pressures of all patients were bellowed 21 mmHg. CONCLUSION: High intraocular pressure with surgery for glaucoma is caused by multiple factors, preoperative and intraoperative avoid as far as possible, postoperative early detection for symptomatic treatment is the key to successful operation. ©, 2015, International Journal of Ophthalmology (c/o Editorial Office). All right reserved.
Hao D.,The First Hospital of Yulin |
Song Y.,The First Hospital of Yulin |
Che Z.,The First Hospital of Yulin |
Liu Q.,The First Hospital of Yulin
Cell Biochemistry and Biophysics | Year: 2014
The objective of this study was to investigate the role of intracellular calcium overload in the in vitro apoptosis of C6 glioma cells mediated by low level ultrasound and hematoporphyrin monomethyl ether (HMME) therapy. The frequency of ultrasound was optimized by the cell viability assay using 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The apoptotic rate, reactive oxygen species (ROS) and decreased mitochondrial membrane potential (MMP) were determined by flow cytometry. Morphological changes were observed by the transmission electron microscope. Concentrations of intracellular Ca2+, [Ca2+]i were detected by a confocal microscopic laser scanning, and the release of cytochrome-c (cyt-c) was measured by western blotting. Results: The SDT-mediated apoptotic effect involved an overload of [Ca2+]i derived from the intra- and extracellular sources during the early progression of apoptotosis. The process was associated with an increased ROS production, a decreased MMP, and a release of cyt-c. In conclusion,the combined use of low level ultrasound and HMME improved the apoptotic rate of C6 glioma cells mediated by ultrasound alone. The [Ca2+]i overload involving activation of mitochondrial signaling played a pivotal role in the SDT-induced apoptosis. © 2014 The Author(s).
PubMed | The First Hospital of Yulin
Type: Journal Article | Journal: Cell biochemistry and biophysics | Year: 2014
The objective of this study was to investigate the role of intracellular calcium overload in the in vitro apoptosis of C6 glioma cells mediated by low level ultrasound and hematoporphyrin monomethyl ether (HMME) therapy. The frequency of ultrasound was optimized by the cell viability assay using 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The apoptotic rate, reactive oxygen species (ROS) and decreased mitochondrial membrane potential (MMP) were determined by flow cytometry. Morphological changes were observed by the transmission electron microscope. Concentrations of intracellular Ca2+, [Ca2+]i were detected by a confocal microscopic laser scanning, and the release of cytochrome-c (cyt-c) was measured by western blotting.The SDT-mediated apoptotic effect involved an overload of [Ca2+]i derived from the intra- and extracellular sources during the early progression of apoptotosis. The process was associated with an increased ROS production, a decreased MMP, and a release of cyt-c. In conclusion,the combined use of low level ultrasound and HMME improved the apoptotic rate of C6 glioma cells mediated by ultrasound alone. The [Ca2+]i overload involving activation of mitochondrial signaling played a pivotal role in the SDT-induced apoptosis.