Li R.,The First Hospital of Xian City |
Dai G.,Yanan University |
Zhao M.,The First Hospital of Xian City |
Zhang Y.,The First Hospital of Xian City |
And 3 more authors.
Experimental and Therapeutic Medicine | Year: 2013
The aim of this study was to investigate the preventative effect of Astragalus flavescens on hepatic fibrosis in rats and its mechanism of action. A total of 60 rats were randomly divided into normal control, model control, high-dose treatment and low-dose treatment groups, and a hepatic fibrosis model was established. The high- and low-dose treatment groups were treated with 2 g/100 g and 0.5 g/100 g Astragalus flavescens, respectively, once a day. Eight weeks following the initiation of treatment, the liver specimens of the rats were stained and observed under a light microscope. Hepatic fibrosis indices, specifically, type III precollagen (PC III), type IV collagen (C IV), hyaluronic acid (HA) and laminin (LN), were detected. Furthermore, the expression and localization of the hepatic fibrosis-related factors transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF) and platelet-derived growth factor-BB (PDGF-BB) were determined. The serum levels of hepatic fibrosis indices, and the liver tissue levels of hepatic fibrosis-related factors and collagen surface density in the model control group and the high- and low-dose treatment groups were significantly higher compared with those of the normal control group (P<0.05). In addition, the values in the two treatment groups were significantly lower compared with those of the model control group (P<0.05). The present study demonstrated that Astragalus flavescens effectively prevents hepatic fibrosis in rats. A possible mechanism for this is that it may reduce the expression levels of TGF-β1, PDGF-BB and CTGF, thereby inhibiting the activation of hepatic stellate cells and specifically blocking the signal transduction pathway of hepatic fibrosis.
Wu P.-A.,The First Hospital of Xian City |
Yan Y.,The First Hospital of Xian City |
Cui J.-R.,The First Hospital of Xian City
International Journal of Ophthalmology | Year: 2011
AIM: To find special staining methods which is fast, accurate and convenient operation for fungal keratitis tissue section. METHODS: Specimens of 50 cases with the clinical diagnosis of suspected fungal keratitis or corneal ulcer were selected. They were conventionally fixed, dehydrated and dipped in wax for 4 μm paraffin sections, then subject to conventional dewaxing to water. Periodic acid-Schiff stain and gomori methenamine silver stain were compaired. RESULTS: Positive detection rate of periodic acid-Schiff stain was 84%, positive detection rate of gomori methenamine silver stain was 79%. Result was statistically significant (P < 0.05). CONCLUSION: Periodic acid-Schiff staining method is simple, fast, accurate, high positive rate and lower cost. It is superior to gomori methenamine silver staining, and worth promoting.