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Jin H.,Capital Medical University | Li C.,The First hospital of Shijiazhuang City | Ge H.,Capital Medical University | Jiang Y.,Capital Medical University | Li Y.,Capital Medical University
Journal of Translational Medicine

Objective: To investigate warning effect of serum miRNA for intracranial aneurysm rupture through microarray hybridization.Methods: 24 were selected from 560 patients in our department and divided into group A, B, C and D. They are aneurysms with daughter aneurysms group, aneurysm without daughter aneurysms group, ruptured aneurysms group and angiography negative group. Then a microarray study was carried out using serum miRNA. Differentially expressed miRNAs were identified. Cluster analysis was performed in order to make the results looks more intuitive and potential gene targets were retrieved from miRNA target prediction databases.Results: Microarray study identified 86 miRNAs with significantly different (p < 0.05) expression levels between three experimental groups and control group. Among them 69 are up-regulated and 17 are down-regulated. All miRNAs in group A are up-regulated, while there are up and down-regulated in group B and C. A total of 8291 predicted target genes are related to these miRNAs. Bioinformatic analysis revealed that several target genes are involved in apoptosis and activation of cells associated with function of vascular wall.Conclusion: Our gene level approach reveals several different serum miRNAs between normal people and aneurysm patients, as well as among different phases of aneurysm, suggesting that miRNA may participate in the regulation of the occurrence and development of intracranial aneurysm, and also have warning effect for intracranial aneurysm rupture. All differently expressed miRNA in group A are up-regulated, which may suggesting protective function of miRNA for intracranial vascular wall. © 2013 Jin et al.; licensee BioMed Central Ltd. Source

Chen Z.,Chinese Peoples Liberation Army | Li H.,Chinese Peoples Liberation Army | Feng J.,Beijing Institute of Microbiology and Epidemiology | Li Y.,The First hospital of Shijiazhuang City | And 11 more authors.
Frontiers in Microbiology

A carbapenem-nonsusceptible Enterobacter aerogenes strain named 3-SP was isolated from a human case of pneumonia in a Chinese teaching hospital. NDM-1 carbapenemase is produced by a pNDM-BJ01-like conjugative plasmid designated p3SP-NDM to account for carbapenem resistance of 3-SP. p3SP-NDM was fully sequenced and compared with all publically available pNDM-BJ01-like plasmids. The genetic differences between p3SP-NDM and pNDM-BJ01 include only 18 single nucleotide polymorphisms, a 1 bp deletion and a 706 bp deletion. p3SP-NDM and pNDM-BJ01 harbor an identical Tn125 element organized as ISAba125, blaNDM-1, bleMBL, δtrpF, dsbC, cutA, δgroES, groEL, ISCR27 and ISAba125. The blaNDM-1 surrounding regions in these pNDM-BJ01-like plasmids have a conserved linear organization ISAba14-aphA6-Tn125-unknown IS, with considerable genetic differences identified within or immediately downstream of Tn125. All reported pNDM-BJ01-like plasmids are exclusively found in Acinetobacter, whereas this is the first report of identification of a pNDM-BJ01-like plasmid in Enterobacteriaceae. © 2015 Zhou. Source

Liu G.,Hebei Medical University | Zhu T.,Hebei Medical University | Cui Y.,Hebei Medical University | Liu J.,Bethune International Peace Hospital | And 3 more authors.
Biomedicine and Pharmacotherapy

Objective: To investigate the correlations between the polymorphism of IGF2BP2, a diabetes predisposing gene, and breast cancer risk in Chinese female with Han nationality. Method: The genomic DNAs were extracted from the peripheral blood drawn from 564 female breast cancer patients and 394 healthy females of Han nationality. The polymorphism of IGF2BP2 gene rs4402960 was detected by a multiplex PCR-Ligase detection reaction (PCR-LDR) assay from genomic DNA. The correlations between genotype and breast cancer risk as well as clinicopathologic characteristics were compared. Results: The differences are significant between genotype distribution of IGF2BP2 gene rs4402960 (P= 0.009) and allele gene frequency from both disease and control groups. Both T gene carriers (GT. +. TT) (OR = 1.462; 95% CI, 1.127-1.897) and T allele gene (OR = 1.382; 95% CI, 1.116-1.710) significantly increase the risk of breast cancer. No considerable correlations were observed between the polymorphism of rs4402960 and the clinical pathological parameters, such as age at diagnosis, lymphatic metastasis, estrogen and progesterone receptor status, cerb-B2 receptor status, and tumor staging. Conclusions: The IGF2BP2 gene rs4402960 polymorphism increases the breast cancer risk of Chinese females with Han nationality, and is a breast cancer predisposing gene. © 2014 Elsevier Masson SAS. Source

Yang G.,Shandong University | Li X.,Shandong University | Cao Y.,Shandong University | Zhao L.,The First hospital of Shijiazhuang City | Wang Y.,Shandong University
International Journal of Clinical and Experimental Medicine

Keloids are scars characterized by pathologically excessive dermal fibrosis and aberrant wound healing. Keloid fibroblasts (KFs) persistently proliferate and fail to undergo apoptosis, no treatment is completely effective against these lesions. Sortilin was found involved in pro-nerve growth factor (proNGF)-induced apoptosis of rat vascular smooth muscle cells. However, the role of sortilin in dermal fibrosis keloids is still unclear. Therefore, in this study, we investigated the effects of sortilin on KFs and proNGF-induced KFs apoptosis and explored the underlying mechanism. We found that sortilin was decreased in KFs, overexpression of sortilin significantly promoted proNGF-induced mitochondria-mediated internal apoptosis of KFs. In addition, sortilin obviously inhibited the activation of PI3K/Akt/NF-κB signaling pathway but increased bax expression. Taken together, our study suggested that sortilin potentiated proNGF-induced apoptosis in KFs through increasing the expression of bax and suppressing the activation of the PI3K/Akt/NF-κB signaling pathway, and sortilin is a potential target for treatment of the keloid disease. © 2016, E-Century Publishing Corporation. All rights reserved. Source

Liu Y.,The First hospital of Shijiazhuang City | Liu K.,Hebei Medical University
Experimental and Therapeutic Medicine

The aim of the present study was to investigate the effects of spironolactone and losartan on the early healing stage of acute myocardial infarction (AMI) in rats. An AMI rat model was established and the rats were randomly divided into four groups: AMI (n=12), AMI + spironolactone (AMI + S; n=12), AMI + losartan (AMI + L; n=12) and AMI + spironolactone combined with losartan (AMI + S + L; n=12). Sham-operated rats served as a control group (n=12). The expression levels of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) in the non-infarcted myocardium surrounding the AMI area were determined using immunohistochemistry. In addition, the capillary density in the non-infarcted myocardium surrounding the AMI area was detected. The capillary densities around the infarcted area in the AMI and treatment groups at day 7 and 14 following AMI surgery were significantly higher compared with the sham-operated rats. Compared with the AMI group, the capillary densities around the infarcted area and the ratio of MMPs/TIMP-1 were increased in the treatment groups following AMI surgery; however, the increased ratio of MMPs/TIMP-1 was reduced at day 14 following AMI surgery. Therefore, these results indicated that spironolactone and losartan may promote the formation of collateral circulation in the non-infarcted tissue surrounding the infarcted area by regulating the production of MMPs. Source

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