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Shen W.,Hebei Medical University | Gao H.,The First Hospital of Shijiazhuang | Zhu S.,Hebei Medical University | Li Y.,Hebei Medical University | And 3 more authors.
Oncology Letters | Year: 2016

The aim of this study was to quantify the radiation dose to the corresponding lymph drainage area in esophageal cancer using three-dimensional conformal radiation therapy (3D-CRT) with involved-field irradiation (IFI) and to analyze associated factors. A retrospective analysis of 81 patients with esophageal cancer was conducted. According to the location of the lesions, the lymph drainage area was delineated and the dosimetric parameters were calculated. The 1-, 3-, 5-and 8-year survival rates of the patients were 67.90, 33.33, 20.99 and 11.11%, respectively. Based on the dose-volume histogram in the treatment plan, we calculated the volume percentage of the planning target volume including clinically positive lymph nodes (PTV-N) receiving radiation doses of 30, 35, 40, 45 and 50 Gy (VPTV-N30-50). The median values of VPTV-N30-50 were 73, 70, 67, 64 and 58%, respectively. The prescribed dose size exhibited no correlation with VPTV-N30-35, but did exhibit a significant correlation with VPTV-N40-50; the radiation field was not correlated with VPTV-N30-45, but exhibited a significant correlation with VPTV-N50; the length of the lesion on esophageal barium meal X-ray and the PTV were significantly correlated with VPTV-N30-50. The analysis of variance revealed that the VPTV-NX value in the upper thoracic segment was higher compared with that in the middle and lower thoracic segments; VPTV-N30-35 values differed significantly according to the different locations of the lesions, whereas VPTV-N40-50 values exhibited no significant differences. The value of VPTV-NX exerted no significant effect on long-term patient survival. Therefore, the corresponding lymph drainage area of esophageal cancer is subjected to a certain radiation dose when patients undergo 3D-CRT with IFI, which may play a role in the prevention of regional nodal metastasis. However, this hypothesis requires confirmation by further clinical studies. © Spandidos Publications 2015. All right reserved. Source

Tang L.-J.,Hebei Medical University | Li Y.,The First Hospital of Shijiazhuang | Liu Y.-L.,Hebei Medical University | Wang J.-M.,Hebei Medical University | And 2 more authors.
Gene | Year: 2016

Ubiquitin-specific protease 12 (USP12) plays a significant role in tumor cell apoptosis and cell cycle progression. However, the regulatory mechanism of USP12 in human cervical carcinoma HeLa cell growth is unknown. In this study, we showed that knockdown of USP12 effectively induced cell cycle arrest in HeLa cells and decreased BMI-1, c-Myc and cyclin D2 transcription levels. By contrast, unlike the inactive C48S mutant, over-expression of USP12 and the deubiquitinase activity enhanced L153S and R237C mutants, had the opposite effects. Interestingly, compared to wild-type, the L153S mutant resulted in a more effective cell cycle-promotion and increased BMI-1, c-Myc and cyclin D2 transcript levels. In addition to BMI-1, USP12 R237C exhibited a functional resemblance to the wild-type by involving c-Myc and cyclin D2. The effect of USP12 on HeLa cell apoptosis was not observed in our study. These results suggest that USP12 may be responsible for HeLa cell growth by affecting cell cycle progression. © 2015 Elsevier B.V. Source

Shan B.,Hebei Medical University | Dong M.,Hebei University of Science and Technology | Tang H.,Hebei Medical University | Wang N.,Hebei Medical University | And 5 more authors.
Oncology Letters | Year: 2014

Voltage-gated sodium channels (VGSCs) are expressed not only in excitable cells but also in numerous metastatic cells, particularly in certain types of cancer cells. In some types of cancer, including prostate cancer, the expression of VGSCs is associated with cancer migration, invasion and metastasis in vivo. However, the detailed expression profiles of VGSC α subunits in normal human prostate, in prostatic hyperplasia and prostatic cancer remain controversial. In the present study, quantitative polymerase chain reaction was used to systematically detect all subtypes of VGSC α subunits in normal human prostate, benign prostatic hyperplasia (BPH) and prostate cancer cells. The expression profile of VGSC α subunits was observed to differ between these cell types. Nav1.5 was the major isoform expressed in normal human prostate tissue, while Nav1.5 and Nav1.2 were the predominant isoforms in BPH tissue. However, in PC-3 and LNCaP cells, two typical prostate cancer cell lines, Nav1.6 and Nav1.7 were abundantly expressed. By comparing the relative expression levels of Nav1.5, Nav1.6 and Nav1.7 in these cells, the mRNA levels of Nav1.6 and Nav1.7 were identified to be 6- to 27-fold higher in PC-3 and LNCaP cells than in either normal or BPH samples (P<0.05); however, Nav1.5 mRNA levels were relatively lower compared with those of Nav1.6 or Nav1.7 in all cells analyzed. To confirm whether Nav1.6 and Nav1.7 expression in cancer cells was functional, a patch-clamp technique was used to record whole-cell currents. A tetrodotoxin-sensitive sodium current was successfully recorded in PC-3 cells, but not in LNCaP cells. It was concluded that although all types of VGSC α subunits exhibited low expression levels in normal prostate and BPH cells, both Nav1.6 and Nav1.7 were significantly upregulated in the prostate cancer cell lines, suggesting these subtypes may be potential diagnostic markers and therapeutic targets for certain types of prostate cancer in humans. Source

Jiang H.,Tangshan Gongren Hospital | Zhang L.-Y.,The First Hospital of Shijiazhuang | Wang S.-Z.,Tangshan Gongren Hospital | Zhang X.-J.,Tangshan Gongren Hospital | Liu X.-H.,Tangshan Gongren Hospital
Chinese Traditional and Herbal Drugs | Year: 2015

Objective: By optimizing the chromatographic conditions, to establish a method of multi-target content analysis, the components of danshensu, protocatechuic aldehyde, salvianolic acid B, puerarin, and berberine hydrochloride in Xiaokeping Tablets were simultaneously determined. Methods: Agilent Zorbax Eclipse XDB-C18 (150 mm × 4.6 mm, 5 μm) chromatographic separation column was used, mobile phase was composed of acetonitrile (A), methanol (B), and 0.5% H3PO4 aqueous solution (C) in a linear gradient elution mode (0 min: A 4%, C 96%; 7 min: A 4%, B 1%, C 95%; 20 min: A 10%, B 3%, C 87%; 30 min: A 15%, B 3%, C 82%; 45 min: A 25%, B 10%, C 65%; and 60 min: A 30%, B 10%, C 60%). The flow rate was 1.0 mL/min, the column temperature was set at 30 ℃, and injection volume was 10 μL. The detection wavelength was set at 280 nm for danshensu, protocatechuic aldehyde, and salvianolic acid B, 250 nm for puerarin, and 265 nm for berberine hydrochloride. Results: There was a good linear relationship over a rang of 33.02-528.41 μg/mL for danshensu, 1.44-22.46 μg/mL for protocatechuic aldehyde, 374.51-5992.20 μg/mL for salvianolic acid B, 84.86-1357.82 μg/mL for puerarin, and 312.50-5000.00 μg/mL for berberine hydrochloride, respectively. The average recovery rates were 98.24% (RSD = 0.26%), 98.37% (RSD = 1.01%), 98.86% (RSD = 0.50%), 98.93% (RSD = 0.68%), 99.04% (RSD = 0.29%), respectively. Conclusion: The separation effect is good, the operation is simple and convenient, so as to achieve the better quality control of the products by this method. ©, 2015, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved. Source

Han X.,Hebei Medical University | Hou S.,The First Hospital of Shijiazhuang | Yang A.,Hebei Medical University
Medical Science Monitor | Year: 2016

Background: Diabetes mellitus a common metabolic disorder with hyperglycemia, is caused by the interaction of genetic and environmental factors. Approximately 12~20% of diabetic patients have risk of colorectal cancer. Recent studies revealed that the insulin-like growth factor system (IGFs) plays an important role in tumor occurrence. This study thus investigated the relationship between IGFs-related proteins in diabetic patients and the incidence of colorectal carcinoma. Material/Methods: A retrospective study was performed in a total of 206 individuals, including 85 diagnosed with diabetes. The incidence of colorectal cancer was tracked, along with the detection of IGFs expression in serum. During the surgical resection, tumor tissues and adjacent tissues were collected and quantified for IGFs expression level. Results: We found no significant difference in age or sex between the diabetic and control groups. Diabetic patients, however, had elevated body weight and higher incidence of colorectal cancer compared to non-diabetic controls (p<0.05). The diabetic group also had higher IGF-I and IGF-IR mRNA levels in serum, while IGFBP-6 expression was down-regulated. In comparison to adjacent healthy tissues, tumor tissue had higher levels of IGF-I and IGF-IR but lower levels of IGFBP-6 (p<0.05). Conclusions: Our study showed higher incidence of colorectal cancer in diabetics compared to non-diabetics. The occurrence of colorectal cancer in diabetic patients may be associated with elevated IGFs-related protein expression level. © Med Sci Monit. Source

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