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Liu R.J.,The First Hospital of Qinhuangdao
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases | Year: 2011

Lipopolysaccharide (LPS) can activate alveolar epithelial cells (AECs) and induce inflammatory injury. Toll-like receptor-4 (TLR-4) is integrally involved in LPS signaling and has a requisite role in the activation of NF-κB. NF-κB is a key intercellular signaling event that mediates cell inflammatory responses. The aim of the study is to investigate in an in vitro model the inflammatory responses of AECs induced by LPS and the probable mechanism underlined the observed inflammatory responses. So cytokines of ICAM-1, TNF-α and IL-8 secreted by LPS-activated AECs were observed. And the initial signal molecule (the expression of TLR-4 mRNA) and the key intracellular steps (the activation of NF-κB) were studied in detail. The study was performed on A549 cells (Human lung adenocarcinoma cell line). A549 cells were divided into two groups: control, and LPS interference group. Proinflammatory cytokines ICAM-1, TNF-α and IL-8 were detected by ELISA or radioimmunological methods. The expression of TLR-4 mRNA was detected by real time PCR. The activation of NF-κB was detected by Western blot (proteins of I-κBα and NF-κB p65). Compared with control group, ICAM-1 and TNF-α of LPS-stimulated group were significantly higher and peaked after 2h before gradually declining at 6 and 12 h; IL-8 was higher after 2 h, which continued up to 12 h. The expression of TLR-4 mRNA of LPS group was significantly higher and peaked after 2 h and gradually declining at 6 and 12 h. Meanwhile, NF-κB was activated after 0.5, 2, 6 and 12 h indicated by the significant degradation of IκB-α and the significant release of NF-κB P65 and its subsequent translocation into the nucleus approximately synchronized. Taken together, the results demonstrate that LPS can induce AECs inflammatory injury via activating TLR-4 and subsequently activating NF-κB. Source


Ejike C.E.C.C.,Michael Okpara University of Agriculture | Yin F.Z.,The First Hospital of Qinhuangdao
Journal of Tropical Pediatrics | Year: 2013

The diagnostic accuracy of blood-pressure-toheight-ratios (BPHRs) in 716 Nigerian children was studied, using standard protocols. Systolic BPHR (SBPHR) and diastolic BPHR (DBPHR) had diagnostic accuracies ranging from 0.934 to 1.000, irrespective of sex and age. Optimal thresholds of SBPHR/DBPHR are provided, and their sensitivities and specificities all exceeded 80%. BPHR is a simple, sensitive and specific tool for the diagnosis of hypertension in this population. © The Author [2012]. Published by Oxford University Press. All rights reserved. Source


Shi G.,Yanshan University | Jin S.,Yanshan University | Li Y.,The First Hospital of Qinhuangdao
ICIC Express Letters, Part B: Applications | Year: 2015

Aiming at relieving the shortage problem of wireless frequency resource, sec- ondary users in cognitive radio networks occupy the spectrum hole opportunistically. In cognitive radio networks, primary users have preemptive priority for the licensed spectrum, and the data transmission of a secondary user is possible to be interrupted by a primary user at any time. For the applications with real-time traffic, the interrupted data is supposed to be discarded by the system. A continuous time queueing model with potential transmission interruption is established accordingly. By using the Laplace transform, the performance measures, such as the average latency, the normalized throughput and the spectrum switching ratio, are presented. The individually and socially optimal strategies are investigated with a non-cooperative game theory, and then a pricing policy for the secondary users is proposed. Experimental results show that the dynamic spectrum allocation scheme with real-time traffic can be optimized socially. © 2015, ICIC International. Source


Tang X.,Southern Medical University | Liu P.,Southern Medical University | Li R.,The First Hospital of Qinhuangdao | Jing Q.,General Hospital of Shenyang Military Command | And 3 more authors.
Basic and Clinical Pharmacology and Toxicology | Year: 2015

Despite advancements in modern medicine, the treatment of acute heart failure (AHF) after acute myocardial infarction (AMI) remains challenging. Milrinone is effective in the treatment of chronic congestive heart failure, but its safety and efficacy in patients with AHF after AMI have not been systematically evaluated. This meta-analysis was performed to assess the safety and efficacy of milrinone in patients with AHF after AMI. We used a pre-designed protocol to search electronic databases for randomized trials assessing milrinone for the treatment of AHF after AMI. Data were abstracted from relevant studies. Heterogeneity was assessed qualitatively using a Q test and quantified using the I2 statistic. Pooled risk estimates with 95% confidence intervals (CIs) were obtained using fixed-effects models unless substantial heterogeneity was observed (I2 ≥ 50% and heterogeneity p ≤ 0.1). Four randomized trials met the inclusion criteria. However, there were no significant differences in deaths, blood pressure, premature ventricular contractions, gastrointestinal reactions, or ventricular tachycardia or fibrillation (all p > 0.05) between control group and milrinone treatment group. Pooled estimates showed that milrinone significantly increased the left ventricular ejection fraction (MD 5.69; 95% CI 4.27 to 7.10; p < 0.00001) and cardiac output (MD 0.35, 95% CI: 0.13 to 0.56; p = 0.002, I2 = 24%). While studies to date are few and limited by small sample sizes and poor quality, they suggest that treatment with milrinone may be safe and effective for patients with AHF after AMI. However, this meta-analysis did not show that milrinone could improve prognosis or the survival rate. © 2015 Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society). Source


Lu H.,The First Hospital of Qinhuangdao | He Y.,Gastroscopy Room | Lin L.,The First Hospital of Qinhuangdao | Qi Z.,B Ultrasound Room | And 3 more authors.
Tumor Biology | Year: 2015

Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a lncRNA playing oncogenic role in several cancers, including cervical cancer. However, its role in radiosensitivity of cervical cancer is not yet well understood. This study explored the role of MALAT1 in radiosensitivity of high-risk human papillomavirus (HR-HPV)-positive cervical cancer and whether there is a ceRNA mechanism which participated in its regulation over radiosensitivity. Based on tissue samples from 50 cervical cancer cases and 25 healthy controls, we found MALAT1 expression was significantly higher in radioresistant than in radiosensitive cancer cases. In addition, MALAT1 and miR-145 expression inversely changed in response to irradiation in HR-HPV+ cervical cancer cells. By using clonogenic assay and flow cytometry analysis of cell cycle distribution and apoptosis, we found CaSki and Hela cells with knockdown of MALAT1 had significantly lower colony formation, higher ratio of G2/M phase block and higher ratio of cell apoptosis. By performing RNA-binding protein immunoprecipitation (RIP) assay and RNA pull-down assay, we confirmed that miR-145 and MALAT1 were in the same Ago2 complex and there was a reciprocal repression between them. Then, we explored the function of MALAT1-miR-145 in radiosensitivity of cervical cancers cells and demonstrated that si-MALAT1 and miR-145 had some level of synergic effect in reducing cancer cell colony formation, cell cycle regulation, and inducing apoptosis. These findings provide an important clue about microRNA-lncRNA interaction in the mechanism of radioresistance of cervical cancer. © 2015 International Society of Oncology and BioMarkers (ISOBM) Source

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