The First Hospital of Putian City

Putian, China

The First Hospital of Putian City

Putian, China
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Chen G.,The First Hospital of Putian City | Wang G.,The First Hospital of Putian City | Li G.,The First Hospital of Putian City
Chinese Journal of Rehabilitation Medicine | Year: 2017

Objective: To observe the bone mineral density for patients with postmenopausal osteoporosis treated by lowmagnitude whole-body vibration and alendronate. Method: A total of sixty postmenopausal osteoporotic women were randomly divided into three groups (WBV n=20, ALE n=20 and WBV+ALE n=20). The level of WBV applied was 0.5-0.8 g at 45-55 Hz for 6 months(30min/d, 5d/week). ALE was administered in dose of 70 mg once a week. The BMD of lumbar (L2-L4), femoral neck, anter, and wards triangle region of hip was determined by dual-energy X-ray absorptiometry before and after treatment. Result: (1) The BMD in lumbar 2-4 after 6-months treatment showed that the average BMD was increased by 3.72% in WBV-group, by 4.55% in ALE-group, and by 7.16% in WBV+ALE-group. There were significant differences among three groups (P<0.05). (2) The BMD in femoral neck after 6-months treatment showed that the average BMD was increased by 2.24% in WBV-group, by 3.43% in ALE-group, and by 7.22% in WBV+ ALE-group. There were significant differences among three groups (P<0.05). (3)The BMD in anter of hip after 6-months treatment showed that the average BMD was increased by 2.10% in WBV-group, by 4.90% in ALE-group, and by 7.63% in WBV+ALE-group. There were significant differences among three groups (P< 0.05). (4) The BMD in wards triangle region of hip after 6-months treatment showed that the average BMD was increased by 5.00% in WBV-group, by 7.13% in ALE-group, and by 9.04% in WBV+ALE-group. There were also significant differences among three groups (P<0.05). Conclusion: Whole-body vibration, alendronate and combination treatment can increase the BMD in postmenopausal osteoporotic women, significantly. Furthermore, whole- body vibration combined with alendronate was more effective in improving the BMD in postmenopausal women.sss © 2017, Editorial Board of Chinese Journal of Rehabilitation Medicine. All right reserved.


Chen G.,The First Hospital of Putian City | Lin Z.,The First Hospital of Putian City | Li G.,The First Hospital of Putian City
Chinese Journal of Rehabilitation Medicine | Year: 2016

Objective: To investigate the different effect between low-magnitude whole-body vibration(WBV) and alendronate( ALE) on bone properties in ovariectomized rats. Method: A total of 104 SD rats were randomly divided into four groups (sham, OVX+VEH, OVX+WBV, OVX+ALE). The level of WBV applied was 0.3g at 45-55Hz for 20 min/d, 5d/week and for 12 weeks. ALE was administered in dose of 1 mg/kg once a week. Every 4 weeks 8 rats from each group were sacrificed and their blood and both tibiae were harvested. The expression of osteocalcin(OC) and C-terminal cross linked telopeptides of type I collagen(CTX) in serum was measured by enzyme-linked immunosorbent assay (ELISA) and the tibiaes were subjected to metaphyseal three-point bending and micro-CT analysis. Result: OC rose after ovariectomy and was not appreciably changed by either ALE or WBV alone or in combination. ALE treatment significantly prevented an increase of CTX. Compared with the OVX+WBV group, nearly all tested indices such as the BV/TV, TV apparent, Tb.N, Tb.Th, and Conn.D were higher in the OVX+ ALE group at week 12. At week 12, tibiae treated with ALE exhibited a significantly higher Fmax, energy absorption and stiffness compared to the OVX+WBV group, but there was no significant difference. Conclusion: Compared with the WBV, ALE was more effective at preventing bone loss and improved the trabecular architecture. © 2016, Editorial Board of Chinese Journal of Rehabilitation Medicine. All right reserved.


Geng Y.-J.,Harbin Medical University | Wu Q.-H.,The First Hospital of Putian City | Zhang R.-Q.,Harbin Medical University
Journal of Clinical Anesthesia | Year: 2017

Study objective To compare the incidence of postoperative cognitive dysfunction (POCD) in elderly surgical patients (> 60 years) receiving different anesthetics (propofol, sevoflurane, or isoflurane) and to identify potential biomarkers of POCD in this patient population. Design Prospective, randomized, double-blind clinical trial. Setting University-affiliated teaching hospital. Patients One hundred and fifty elderly patients scheduled for laparoscopic cholecystectomy. Interventions Elderly patients undergoing laparoscopic cholecystectomy were randomly assigned to receive propofol, sevoflurane, or isoflurane anesthesia. Measurements: Cognitive function was assessed using neuropsychological tests at baseline (1 day before surgery [D0]), and on postoperative day 1 (D1) and day 3 (D3). Plasma S-100β and Aβ1–40 protein, IL-1β, IL-6 and TNF-α concentrations were assessed before induction of anesthesia (T0), after extubation (T1), and 1 h (T2) and 24 h (T3) postoperatively. Main results The incidence of POCD was significantly lower in the propofol group compared to the isoflurane group and the sevoflurane group at D1 and D3 (propofol vs. isoflurane: D1 and D3, P < 0.001; propofol vs. sevoflurane: D1, P = 0.012; D3, P = 0.013). The incidence of POCD was significantly lower in the sevoflurane group compared to the isoflurane group at D1 ( P = 0.041), but not at D3. Postoperatively, plasma S-100β and Aβ1–40 protein, IL-1β, IL-6, and TNF-α concentrations were significantly decreased in the propofol group compared to the isoflurane group. Conclusions Propofol anesthesia may be an option for elderly surgical patients. © 2017


Wei M.,Guangzhou University | Wu Z.Y.,Guangzhou University | Lin J.H.,Guangzhou University | Li Y.,Guangdong Academy of Agriculture science | And 4 more authors.
Genetics and Molecular Research | Year: 2015

In this study, we identified potential serum biomarkers for the diagnosis of active tuberculosis (TB) and screening for latent TB infections (LTBIs). Peripheral blood samples from 40 healthy individuals, 40 patients with TB, and 40 LTBI individuals were stimulated with the TB-specific antigens ESAT-6 and CFP-10. Human inflammatory cytokine arrays were used to detect the expression of inflammatory cytokines. Cytokines with significant changes were screened to construct a cytokine regulation network. The levels of the cytokines CCL1 (I-309), CXCL9 (MIG), IL- 10, IL-6, CSF2, CSF3, IL-8, IL-1α, IL-7, TGF-β1, CCL2, IL-2, IL-13, and TNFα were significantly upregulated in the active TB group. The levels of CCL3, IL-1β, CCL8, IFNγ, and CXCL10 were significantly increased in the TB groups compared to those in the healthy control group. sTNF RII was upregulated in the LTBI group. CCL4 and MIP1d were significantly increased in all groups.The upregulated cytokines were mainly found in the IFNγ and IL-1α regulatory networks. Importantly, we found that CXCL10 (IP-10), CCL3, CCL8, and IL-1β may be more suitable than IFNγ for active or latent TB infection screening. Furthermore, we found that levels of CCL1 (I-309), CXCL9 (MIG), IL-10, IL-6, CSF2, CSF3, IL-8, IL-1α, IL-7, TGF-β1, CCL2, IL-2, and IL-13 after TB antigen stimulation may help distinguish between active and latent TB. © FUNPEC-RP.


Zheng H.-Y.,Fujian Medical University | Zheng H.-Y.,The First Hospital of Putian City | Lin W.-Q.,The First Hospital of Putian City | Hu J.-D.,Fujian Medical University | And 2 more authors.
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology | Year: 2015

OBJECTIVE: To investigate the apoptosis-inducing effects of emodin on multidrug resistant leukemia cell line K562/Adr, and to explore the role of Akt-Caspase 3 signal pathway in apoptosis of K562/Adr cells treated with emodin.METHODS: K562/Adr cells were exposed to emodin of different doses. The ability of emodin to induce apoptosis of K562/Adr cells was detected by Annexin V/PI double labeled flow cytometry and DNA ploidy analysis, the expressions of procaspase-3, PARP, Akt, p-Akt protein were determined by Western blot.RESULTS: Apoptosis in K562/Adr cells could be induced by emodin in a dose dependent manner, Western blot results showed that emodin down-regulated the expression levels of procaspase-3, Akt, p-Akt, PARA 116 KD in treated K562/Adr cells, up-regulated expressions leves of PARP 85 KD in a time-dependent manner.CONCLUSION: The Akt-Caspase 3 signal pathway may be involved in these processes.


Wang X.-Y.,Xinjiang Medical University | Ji B.,Xinjiang Medical University | Li B.-B.,the First Hospital of Putian City | Bi X.-J.,Xinjiang Medical University | Liu L.-Z.,Xinjiang Medical University
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Can adipose-derived mesenchymal stem cells be used to treat sensorineural deafness caused by hair cell degeneration and loss? OBJECTIVE: To investigate the effect of adipose-derived mesenchymal stem cells from guinea pigs transplanted via the scala tympani on the recovery from sensorineural hearing in an animal model. METHODS: Intraperitoneal injection of gentamicin was performed to prepare sensorineural deafness models in guinea pigs. Then, adipose-derived mesenchymal stem cells from guinea pig were transplanted via the scala tympani. After 1 and 3 weeks, auditory brainstem responses were detected to observe the hearing variation after adipose-derived mesenchymal stem cell transplantation. Meanwhile, the migration and distribution of EDU-labeled adipose-derived mesenchymal stem cells in the cochlea were traced. RESULTS AND CONCLUSION: After 1 and 3 weeks of implantation, the results from auditory brainstem response showed that the hearing was gradually improved. At 1 week of implantation, most of the cells were distributed in the perilymph and some cells migrated to the Corti organ and attached to the basement membrane. At 3 weeks of implantation, cells migrated and attached to the basement membrane of Corti organ, furthermore, some cells migrated to the cochlear nerve. The longer the time of implantation, the less cell survived. The results indicate that adipose-derived mesenchymal stem cells from guinea pig that are transplanted via the scala tympani can directly migrate and survive ultimately to improve the hearing. © 2014, Chinese Journal of Tissue Engineering Research. All Rights Reserved.


Chen S.-J.,The First Hospital of Putian City | Huang Z.-H.,The First Hospital of Putian City | Guo C.-S.,The First Hospital of Putian City
World Chinese Journal of Digestology | Year: 2014

AIM: To compare the effect of endoscopic colorectal stenting vs ileus tube drainage for treatment of acute malignant colorectal obstruction.RESULTS: The technical success rate of colorectal stenting was 96.3%, and that of ileus tube drainage was 100%. Relief of symptoms occurred within 1-4 d. Thirty-eight patients underwent subsequent elective surgical resection 6-8 d after obstruction relief, without complications of anastomotic leakage or intraperitoneal infection.METHODS: With the aid of fluoroscopy, 45 patients with acute malignant colorectal obstruction were treated by endoscopic colorectal stenting (n = 27) or ileus tube drainage (n = 18). Postoperative remission and complications were compared between the two groups.CONCLUSION: Preoperative stenting and ileus tube drainage in acute colonical obstruction can effectively relieve obstruction and improve the quality of life, although each has its own advantages and disadvantages. © 2014 Baishideng Publishing Group Inc. All rights reserved.


Lin M.,The First Hospital of Putian City | Su J.,Fujian Medical University | Cai W.,Fujian Medical University | Zheng L.,Fujian Medical University | And 2 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011

BACKGROUND: Adrenomedullin is one of potential growth factor that can promote cellular differentiation, growth and reduce apoptosis. OBJECTIVE: To investigate the possibility of bone marrow mesenchymal stem cells transfected by adenovirus vector carrying adrenomedullin (Ad-ADM), and to determine the protein expression of adrenomedullin following transfection. METHODS: The 293A cells were cultivated and the Ad-ADM was amplified. The experiment was divided into three groups: control, blank vector and Ad-ADM transfection. Cells in three groups were cultured with serum-free L-DMEM, Ad-lacZ and Ad-ADM, respectively after they reached 50%-60% confluence. The virus titer of amplified Ad-ADM was detected by plaque methods. Bone marrow mesenchymal stem cells were isolated and expanded using the preplating methods. The efficiency of Ad-GFP transfected mesenchymal stem cells in vitro was determined. Adrenomedullin mRNA expression was detected with reverse transcription-polymerase chain reaction following transfection. RESULTS AND CONCLUSION: The virus titer of amplified Ad-ADM was 1.1×10 9 pfu/mL by plaque methods. After bone marrow mesenchymal stem cells were infected with Ad-ADM, the expression of adrenomedullin mRNA was significantly increased. The expression of adrenomedullin mRNA was not observed in the control group and empty vector group. Quantitative results showed that the expression gradually increased from day 1, reached the peak at day 7, and was still detectable at day 11. The Ad-ADM can successfully transfect bone marrow mesenchymal stem cells. Following transfection, bone marrow mesenchymal stem cells highly express adrenomedullin.


PubMed | Fujian Medical University and The First Hospital of Putian City
Type: Journal Article | Journal: Zhongguo shi yan xue ye xue za zhi | Year: 2015

To investigate the apoptosis-inducing effects of emodin on multidrug resistant leukemia cell line K562/Adr, and to explore the role of Akt-Caspase 3 signal pathway in apoptosis of K562/Adr cells treated with emodin.K562/Adr cells were exposed to emodin of different doses. The ability of emodin to induce apoptosis of K562/Adr cells was detected by Annexin V/PI double labeled flow cytometry and DNA ploidy analysis, the expressions of procaspase-3, PARP, Akt, p-Akt protein were determined by Western blot.Apoptosis in K562/Adr cells could be induced by emodin in a dose dependent manner, Western blot results showed that emodin down-regulated the expression levels of procaspase-3, Akt, p-Akt, PARA 116 KD in treated K562/Adr cells, up-regulated expressions leves of PARP 85 KD in a time-dependent manner.The Akt-Caspase 3 signal pathway may be involved in these processes.


PubMed | Fujian Medical University, Tongji University and the First Hospital of Putian City
Type: | Journal: Frontiers in bioscience (Landmark edition) | Year: 2016

Accumulating evidence suggests that the anti-diabetic drug, metformin, exerts anti-proliferative effects in many types of cancers. However, the function and mechanisms of metformin in human colorectal cancer (CRC) remain unknown. Here, we show that metformin induces growth inhibition and apoptosis through activating AMPK-mTOR pathway in human colorectal cancer cells. Notably, metformin treatment significantly up-regulated adenosine A1 receptor (ADORA1) expression in human colorectal cancer cells, while suppression of ADORA1 activity by its specific inhibitor rescued the growth inhibition induced by metformin. Moreover, ADORA1-mediated growth inhibition and apoptosis induced by metformin is AMPK-mTOR pathway dependent in human colorectal cancer cells. Taken together, these results indicate that metformin suppresses human colorectal cancer growth by inducing apoptosis via ADORA1, which provide evidence the anti-neoplastic effects of metformin in the treatment of human colorectal cancer.

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