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Wei M.,Guangzhou University | Wu Z.Y.,Guangzhou University | Lin J.H.,Guangzhou University | Li Y.,Guangdong Academy of Agriculture science | And 4 more authors.
Genetics and Molecular Research | Year: 2015

In this study, we identified potential serum biomarkers for the diagnosis of active tuberculosis (TB) and screening for latent TB infections (LTBIs). Peripheral blood samples from 40 healthy individuals, 40 patients with TB, and 40 LTBI individuals were stimulated with the TB-specific antigens ESAT-6 and CFP-10. Human inflammatory cytokine arrays were used to detect the expression of inflammatory cytokines. Cytokines with significant changes were screened to construct a cytokine regulation network. The levels of the cytokines CCL1 (I-309), CXCL9 (MIG), IL- 10, IL-6, CSF2, CSF3, IL-8, IL-1α, IL-7, TGF-β1, CCL2, IL-2, IL-13, and TNFα were significantly upregulated in the active TB group. The levels of CCL3, IL-1β, CCL8, IFNγ, and CXCL10 were significantly increased in the TB groups compared to those in the healthy control group. sTNF RII was upregulated in the LTBI group. CCL4 and MIP1d were significantly increased in all groups.The upregulated cytokines were mainly found in the IFNγ and IL-1α regulatory networks. Importantly, we found that CXCL10 (IP-10), CCL3, CCL8, and IL-1β may be more suitable than IFNγ for active or latent TB infection screening. Furthermore, we found that levels of CCL1 (I-309), CXCL9 (MIG), IL-10, IL-6, CSF2, CSF3, IL-8, IL-1α, IL-7, TGF-β1, CCL2, IL-2, and IL-13 after TB antigen stimulation may help distinguish between active and latent TB. © FUNPEC-RP. Source


Lin M.,The First Hospital of Putian City | Su J.,Fujian Medical University | Cai W.,Fujian Medical University | Zheng L.,Fujian Medical University | And 2 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011

BACKGROUND: Adrenomedullin is one of potential growth factor that can promote cellular differentiation, growth and reduce apoptosis. OBJECTIVE: To investigate the possibility of bone marrow mesenchymal stem cells transfected by adenovirus vector carrying adrenomedullin (Ad-ADM), and to determine the protein expression of adrenomedullin following transfection. METHODS: The 293A cells were cultivated and the Ad-ADM was amplified. The experiment was divided into three groups: control, blank vector and Ad-ADM transfection. Cells in three groups were cultured with serum-free L-DMEM, Ad-lacZ and Ad-ADM, respectively after they reached 50%-60% confluence. The virus titer of amplified Ad-ADM was detected by plaque methods. Bone marrow mesenchymal stem cells were isolated and expanded using the preplating methods. The efficiency of Ad-GFP transfected mesenchymal stem cells in vitro was determined. Adrenomedullin mRNA expression was detected with reverse transcription-polymerase chain reaction following transfection. RESULTS AND CONCLUSION: The virus titer of amplified Ad-ADM was 1.1×10 9 pfu/mL by plaque methods. After bone marrow mesenchymal stem cells were infected with Ad-ADM, the expression of adrenomedullin mRNA was significantly increased. The expression of adrenomedullin mRNA was not observed in the control group and empty vector group. Quantitative results showed that the expression gradually increased from day 1, reached the peak at day 7, and was still detectable at day 11. The Ad-ADM can successfully transfect bone marrow mesenchymal stem cells. Following transfection, bone marrow mesenchymal stem cells highly express adrenomedullin. Source


Zheng H.-Y.,Fujian Medical University | Lin W.-Q.,The First Hospital of Putian City | Hu J.-D.,Fujian Medical University | Lin M.-H.,Fujian Medical University | Xie L.-J.,The First Hospital of Putian City
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology | Year: 2015

OBJECTIVE: To investigate the apoptosis-inducing effects of emodin on multidrug resistant leukemia cell line K562/Adr, and to explore the role of Akt-Caspase 3 signal pathway in apoptosis of K562/Adr cells treated with emodin.METHODS: K562/Adr cells were exposed to emodin of different doses. The ability of emodin to induce apoptosis of K562/Adr cells was detected by Annexin V/PI double labeled flow cytometry and DNA ploidy analysis, the expressions of procaspase-3, PARP, Akt, p-Akt protein were determined by Western blot.RESULTS: Apoptosis in K562/Adr cells could be induced by emodin in a dose dependent manner, Western blot results showed that emodin down-regulated the expression levels of procaspase-3, Akt, p-Akt, PARA 116 KD in treated K562/Adr cells, up-regulated expressions leves of PARP 85 KD in a time-dependent manner.CONCLUSION: The Akt-Caspase 3 signal pathway may be involved in these processes. Source


Chen G.-X.,The First Hospital of Putian City | Wang G.-R.,The First Hospital of Putian City | Lin Z.-J.,The First Hospital of Putian City | Li G.-S.,The First Hospital of Putian City | And 6 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Studies have shown that low-intensity and short-time vibration with a certain frequency can reduce the absorption of bone tissue and increase the quantity and quality of bone through promoting the proliferation and differentiation of osteoblasts. Objective: To investigate the effects of different frequencies of vibration strains on cycle, proliferation and differentiation potency of RAW264.7 cells cultured in vitro. Methods: Passage 6 RAW264.7 cells in good conditions were randomly divided into six groups, and each group was induced cultured with Dulbecco's modified Eagle's medium containing receptor activator of nuclear factor kappa B ligand. The final concentration of receptor activator of nuclear factor kappa B ligand was adjusted to 50 μg/L, and then kept without changes. The non-loading group did not loaded with vibration strain, and the other five groups were loaded with 3-10 Hz, 15-35 Hz, 35-45 Hz, 50-70 Hz and 70-90 Hz vibration strains on the RAW264.7 cells respectively. The other vibration parameters were consistent; the vibration time was 15 min/time with the vibration intensity of 0.3 g, twice per day. The cell cycle and cell proliferation were detected at 3 and 6 days after loading of vibration strains. Results and Conclusion: After composite vibration loading for 6 days, the cell cycle phase in the vibration groups was changed to some extent when compared with the non-loading group. Compared with non-loading group, the cell number in the G1 phase of the vibration group was significantly increased (P < 0.01); the cell number in the S phase and G2+M phase of the vibration group was significantly decreased (P < 0.01); the vibration parameters in the vibration group were significantly decreased (P < 0.01). The results indicate that different frequencies of vibration strains can affect the cell cycle and proliferation, and can inhibit the proliferation and differentiation of osteoblasts. Source

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