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PubMed | The First Hospital of Handan City and Hebei Medical University
Type: Journal Article | Journal: International journal of oncology | Year: 2016

The present study evaluated the ability and optimal concentration of tetramethylpyrazine (TMP) to induce human umbilical cord-derived mesenchymal stem cells (hUMSCs) to differentiate into neuronlike cells invitro. Human umbilical cords from full-term caesarean section patients were used to obtain hUMSCs by collagenase digestion after removal of the umbilical artery and vein. The surface antigen expression profile of cultured hUMSCs was monitored by flow cytometry. After amplification, cells of the 5thpassage were divided into experimental groupsAC treated with TMP at4.67, 2.34and1.17mg/ml, respectively, in low glucoseDulbeccos Modified Eagles Medium (LDMEM) (induction medium), while groupD (control) was exposed to LDMEM culture medium only. Differentiation of hUMSCs into neuronlike cells and morphological changes were observed every 0.5h with an inverted phase contrast microscope for 6h. After the 6h induction period, proportions of cells expressing neuronal markers neuronspecific enolase (NSE), neurofilament protein (NFH) and glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. The optimal concentration of TMP was selected on the basis of neuronlike cell positive rate. Western blotting and RTpolymerase chain reaction were applied to detect the expression of NSE, NFH, and GFAP of the group of optimal concentration in each pointintime. Results showed that most primary cells were adherent 12h after seeding and first appeared as diamond or polygon shapes. Thereafter, they gradually grew into long spindleshaped cells and finally in a radiating or swirling pattern. The cells maintained a strong proliferative capacity after continuous passage. Flow cytometry analysis of cultured hUMSCs at the3rd, 5thand10thpassages expressed CD73, CD90 and CD105, but not CD11b, CD19, CD34, CD45 or human leukocyte antigenDR. After 6h of TMP treatment, typical neuronlike cells with many protrusions connected into a netlike pattern were observed in all experimental groups. These neuronlike cells were positive for NSE and NFH, but negative for GFAP. Among the tested treatment groups, groupA with TMP at 4.67mg/ml had the highest expression of NSE and NFH. By contrast, no change was found after induction in the control group. The mRNA expression of cells expressing neuronal markers as well as GAPDH was observed, with the relative NSE transcript levels of0, 1.3030.031, 1.5580.025, 1.9270.019and2.4150.033 after 0, 1, 2, 4and6h of treatment, respectively; the mRNA expression of NHF was0, 1.4290.025, 1.5510.024, 1.9300.042and1.3980.014 after0, 1, 2, 4and6h of treatment, respectively. There was no expression of GFAP before or after induction and all the groups showed high expression of GAPDH at each time point. Protein expression was also observed on cells expressing neuronal markers aswellas GAPDH at each time point. The protein expression of NSE was0, 0.7170.097, 0.9190.056, 1.0970.143and1.1570.055 in proper order; the protein expression of NHF was0, 0.7800.103, 0.9730.150, 1.0530.107and0.7530.094 in proper order. There was no expression of GFAP before or after induction, and all the groups showed high expression of GAPDH at each tested time point. Our results demonstrated that TMP can induce hUMSCs to differentiate into neuronlike cells effectively with the optimal concentration of 4.67mg/ml. After induction, the NSE and NF-H of the neuron-like cells were positive, but the GFAP-2 was negative.


PubMed | The First Hospital of Handan City and Hebei Medical University
Type: Journal Article | Journal: International journal of molecular medicine | Year: 2015

In the present study, human umbilical cord-derived mesenchymal stem cells(hUMSCs) were investigated for their potential to be induced to differentiate invitro into neuron-like cells by monosialoteterahexosyl ganglioside(GM1). Mononuclear cells obtained from umbilical cords from women with full-term pregnancies whose babies were delivered by cesarean section were cultivated invitro and their surface antigen expression profiles were monitored. Following amplification, the cells were divided into 5groups, of which 4(groupsA-D) were treated with GM1 at doses of 50, 100, 150 and 200g/ml, respectively. The control (groupE) was treated with the vehicle only. The ability of GM1 to induce the differentiation of the hUMSCs into neuron-like cells was monitored for 6h. The expression levels of microtubule-associated protein-2(MAP-2), neurofilament protein (NF-H) and glial fibrillary acidic protein(GFAP) were measured by immunohistochemistry. Following exposure to GM1, the hUMSCs first appeared to have a diamond or polygonal shape and gradually grew into long spindle-shaped cells, finally exhibiting a radiating or swirling pattern. The cells maintained a strong proliferative capacity after continuous passage. Flow cytometry revealed that the hUMSCs expressed CD73, CD90 and CD105 up to passage10, but not CD11b, CD19, CD34, CD45 or HLA-DR. Treatment with GM1 for 6h led to the appearance of neuron-like cells with oval-shaped cell bodies and protruding neurites. These neuron-like cells were positive for MAP-2 and NF-H, but negative for GFAP expression. No changes in the expression of these markers were observed in the control group. Thus, the findings of the present study demonstrate that GM1 effectively induces hUMSCs to differentiate into neuron-like cells.


Yang X.L.,The First Hospital of Handan City
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2010

To observe the efficacy of acupoint catgut embedding therapy combined medication on chronic urticaria induced by Helicobacter pylori (HP) infection. Ninety-two cases were randomly divided into 3 groups, named a medication group (group A, 31 cases), an acupoint catgut embedding group (group B, 30 cases) and a medication combined acupoint catgut embedding group (group C, 31 cases). In group A, the medication was administered orally for antihistamine and anti-HP infection. In group B, catgut embedding was applied on Quchi (LI 11), Xuehai (SP 10), Zusanli (ST 36), etc. In group C, acupoint catgut embedding therapy was applied in combination with medication (medication as group A, acupoint catgut embedding as group B). After 3-month treatment, the efficacy, recurrence rate and HP negative rate were compared among 3 groups. Separately, the effective rates of group A, B, C were 61.3% (19/31), 53.3% (16/30) and 90.3% (28/31); the recurrence rates were 27.3% (3/11), 33.3% (3/9) and 5.9% (1/17); and HP negative rates were 31.3% (10/31), 26.7% (9/30) and 77.4% (24/31). The clinical efficacy and HP negative rate in group C were superior to those in group A and B (P < 0.01, P < 0.05). Acupoint catgut embedding therapy combined medication is significant in efficacy and low in recurrence rate in treatment of chronic urticaria caused by HP infection.


Yang X.-L.,The First Hospital of Handan City | Lu S.-J.,The First Hospital of Handan City | Xue J.,The First Hospital of Handan City | Wu Y.-F.,The First Hospital of Handan City | Shi J.-L.,The First Hospital of Handan City
Oncology Letters | Year: 2014

Clear cell sarcoma of soft tissues is a very rare, malignant soft tissue tumor that usually arises in the extremities, with a predilection for the lower limbs. This report presents a 45-year-old male with a painless mass in the right lumbar region for one year. Magnetic resonance imaging showed a 3.6×3.2×1.5-cm soft tissue mass of the right lumbar region. The tumoral cells had pleomorphic nuclei and large amounts of clear cytoplasm, and a proportion of the cells contained melanin. Immunohistochemical analysis was performed, which identified that the cells were positive for S-100, MITF and HMB-45 tumor markers. The patient underwent a postoperative chemotherapy protocol and had no local recurrences at one year post-surgery.


Song H.-Y.,The First Hospital of Handan City | Li J.-X.,North Sichuan Medical College | Liu J.,University of Sichuan | Zheng J.-Q.,University of Sichuan | And 3 more authors.
World Chinese Journal of Digestology | Year: 2014

Aim: To evaluate the feasibility and efficacy of patient-controlled analgesia and sedation (PCAS) with remifentanil-propofol mixture for colonoscopy. Methods: Eighty patients were randomly allocated into either a PCAS group or a total intravenous anesthesia (TIVA) group. In the PCAS group, remifentanil-propofol mixture was pumped at 0.01 mL/(kg•min) after an initial bolus of 3 mL of the mixture (1 mL mixture containing propofol 3 mg and remifentanil 10 μg). The amount of each bolus delivered was 1 mL and the lockout time was 1 min. In the TIVA group, patients received fentanyl 1 μg/kg, midazolam 0.02 mg/kg and propofol (dosage titrated). Cardiorespiratory parameters and auditory evoked response index (AAI) were continuously monitored during the procedure. Recovery from anesthesia was assessed with the Aldrete scale and the Observer’s Assessment of Alertness/Sedation Scale. Visual analogue scale (VAS) was used to assess the satisfaction of patients and endoscopists. Results: No statistical differences were observed in the VAS score of patients (9.58 vs 9.50) or satisfaction of endoscopists (9.43 vs 9.30) between the two groups. A significant decline in mean arterial blood pressure and AAI was recorded in the TIVA group (P < 0.05). Recovery time was significantly shorter in the PCAS group (P < 0.01). Conclusion: PCAS with remifentanilpropofol mixture provides better hemodynamic stability, lighter sedation and faster recovery compared with TIVA. © 2014 Baishideng Publishing Group Inc. All rights reserved.


PubMed | The First Hospital of Handan City
Type: Journal Article | Journal: Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2011

To observe the efficacy of acupoint catgut embedding therapy combined medication on chronic urticaria induced by Helicobacter pylori (HP) infection.Ninety-two cases were randomly divided into 3 groups, named a medication group (group A, 31 cases), an acupoint catgut embedding group (group B, 30 cases) and a medication combined acupoint catgut embedding group (group C, 31 cases). In group A, the medication was administered orally for antihistamine and anti-HP infection. In group B, catgut embedding was applied on Quchi (LI 11), Xuehai (SP 10), Zusanli (ST 36), etc. In group C, acupoint catgut embedding therapy was applied in combination with medication (medication as group A, acupoint catgut embedding as group B). After 3-month treatment, the efficacy, recurrence rate and HP negative rate were compared among 3 groups.Separately, the effective rates of group A, B, C were 61.3% (19/31), 53.3% (16/30) and 90.3% (28/31); the recurrence rates were 27.3% (3/11), 33.3% (3/9) and 5.9% (1/17); and HP negative rates were 31.3% (10/31), 26.7% (9/30) and 77.4% (24/31). The clinical efficacy and HP negative rate in group C were superior to those in group A and B (P < 0.01, P < 0.05).Acupoint catgut embedding therapy combined medication is significant in efficacy and low in recurrence rate in treatment of chronic urticaria caused by HP infection.


PubMed | the First Hospital of Handan City
Type: Journal Article | Journal: Zhongguo fei ai za zhi = Chinese journal of lung cancer | Year: 2011

Survivin, a member of the inhibitor of apoptosis (IAP) protein family, has been demonstrated as a potential new target for apoptosis-based therapy in cancer and lymphoma. The aim of this study is to investigate effects and mechanisms of survivin siRNA transfection on lung adenocarcinoma cell lines SPCA1 and SH77.A siRNA plasmid expression vector and pSi scrambled against survivin were constructed and transfected into SPCA1 and SH77 cells with Lipofectamine 2000. The proliferations of lung adenocarcinoma SPCA1 and SH77 cells were detected by MTT. The apoptotic rate and cell cycle were detected by flow cytometer. The activity of survivin mRNA and protein expression were analyzed with RT-PCR and Western blot.Survivin siRNA reduced the proliferation of SPCA1 and SH77 cells. Cell cycle was inhibited in G0/G1. Expressions of survivin siRNA mRNA and protein were reduced in transfected cells compared with the control cells.siRNA targeted against survivin can effectively suppress SPCA1 and SH77 cells proliferation and significantly induce SPCA1 and SH77 cells apoptosis.

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