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Zhou J.,The Affiliated Hospital of Jining Medical College | Zhou J.,Zunyi Medical College | Ma R.,Zunyi Medical College | Luo R.,Zunyi Medical College | And 6 more authors.
Cancer Epidemiology

Background: The immunoscope spectratyping to TRBV CDR3 had been applied in infectious diseases, tumors, autoimmune diseases and so on, this study aimed to primarily explore CDR3 spectratyping and molecular features of TCR β chain in the peripheral blood and tissue of patients with colorectal carcinoma. Methods: Blood and tissue samples were collected from seven patients with colorectal carcinoma (CRC), while blood samples were also collected from two healthy controls as control. Using the real-time fluorescence quantitative reverse transcription polymerase chain reaction (RQ-PCR) and DNA melting curve analysis techniques, the features of T-cell receptor beta chain variable region (TRBV) were determined. Results: The gene melting spectral pattern (GMSP) of 24 TRBV gene families exhibited a highly diverse multimodal shape for most of the TRBV gene families, compared to healthy controls, the more GMSP of patients with CRC showed either a single peak, or several prominent melting peaks (skewed) for certain TRBV gene families. Different patients have different skewed patterns. In the analysis results of sequence, some TRBV CDR3 gene families showed sharing the same motif, such as, TRBV6 of P5, TRBV13.1 of P6 and TRBV21 of P7 (tissue sample) shared the same motif 'GT'; TRBV1 of P1 and TRBV21 of P7 shared the same motif 'AGG', TRBV11 of P1, TRBV21 of P5 and TRBV21 of P7 (tissue sample) shared the same motif 'TDTQY', and even TRBV21 of P5 and P7 (tissue sample) shared the large motif 'SGTDTQY'. As a whole, most of TRBV gene families have the similar motifs 'X-Q', the nucleotide 'X' mainly was 'E', it also was possible be 'T', 'G' or 'K' in some CDR3 gene families of patients with CRC. Conclusions: There were different GMSPs in different patients with CRC, CDR3 spectratyping and the molecular features of TCR β chain in the peripheral blood and tissue of patients with CRC were not same or similar, this information would provide ideas for individualized therapy to CRC. © 2010. Source

Feng Y.H.,the First Affiliated Hospital of Zunyi Medical College
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology

The aim of this study was to investigate the expression of matrix metalloproteinase 26 (MMP-26), tissue inhibitor of metalloproteinase-4 (TIMP-4) and matrix metalloproteinase 9 (MMP-9) in patients with diffuse large B cell lymphoma (DLBCL) and their correlations with pathogenesis and development of DLBCL. A total of 95 specimens excised from DLBCL patients were prepared. Expression of MMP-26, TIMP-4 and MMP-9 were tested by SABC immunohistochemistry method and its correlation to clinicopathology indexes were analyzed. The results showed that as compared with reactive hyperplasia of lymph nodes, the high expression of MMP-26, TIMP-4 and MMP-9 were found in different types of DLBCL. The positive expression rate of MMP-26 was related to immune typing (P < 0.05). The expression level of MMP-26 in GCB was lower than that in non-GCB, and did not relate to clinical staging, age, sex, diseased region (P > 0.05). The positive expression rate of MMP-9 was related to clinical staging, the positive expression rate of MMP-9 proteins in patient at III and IV stage was obviously higher than that in patients at I and II stage, but did not relate to immune type, age, sex and diseased region of DLBCL (P > 0.05). The expression of TIMP-4 did not relate to immune type, clinical stage, age, sex, disease region (P > 0.05). The expression of MMP-26 in pathologic tissue of DLBCL did not relate to expression of TIMP-4, but positively related to expression of MMP-9 protein (r = 0.486, P < 0.05). It is concluded that MMP-26 and MMP-9 synergically express in DLBCL. MMP-26 may be involve in pathogenesis and invasiveness of DLBCL, the expression of MMP-26 relates to subtypes of DLBCL. The MMP-26 may serve as an indicator for typing of DLBCL and contributes to predict the invasion and metastasis of DLBCL and itself may become a potential target for therapy. Source

Zhong J.,Chongqing Medical University | Jiang L.,Chongqing Medical University | Cheng C.,Chongqing Medical University | Huang Z.,Chongqing Medical University | And 7 more authors.
Brain Research

Background and objective The present study aims to detect the altered lncRNA expression in the mouse cortex after traumatic brain injury (TBI). We also simultaneously detected the altered mRNA profile to further analyze the possible function of lncRNA. Method C57BL/6 mice (n=18) were used to construct a controlled cortical impact model. At 24 h post-TBI, the cortex around injury site was collected and the total RNA was extracted to construct the cDNA library. RNA sequencing (RNA-seq) was carried out followed by RT-PCR for confirmation. Bioinformatic analysis (including GO analysis, KEGG pathway and co-expression analysis) also were performed. Results A total of 64,530 transcripts were detected in the current sequencing study, in which 27,457 transcripts were identified as mRNA and 37,073 transcripts as lncRNA. A total of 1580 mRNAs (1430 up-regulated and 150 down-regulated) and 823 lncRNAs (667 up-regulated and 156 down-regulated) were significantly changed according to the criteria ( |log2 (fold change)|>1 and P<0.05). These altered mRNAs were mainly related to inflammatory and immunological activity, metabolism, neuronal and vascular network. The expression of single lncRNA may be related with several mRNAs, and so was the mRNA. Also, a total of 360 new mRNAs and 8041 new lncRNAs were identified. The good reproducibility and reliability of RNA-seq were confirmed by RT-PCR. Conclusion Numerous lncRNAs and mRNAs were significantly altered in mouse cortex around the injury site 24 h after TBI. Our present data may provide a promising approach for further study about TBI. © 2016 Elsevier B.V. Source

Yu A.,the First Affiliated Hospital of Zunyi Medical College | Duan H.,the First Affiliated Hospital of Zunyi Medical College | Zhang T.,the First Affiliated Hospital of Zunyi Medical College | Pan Y.,the First Affiliated Hospital of Zunyi Medical College | And 5 more authors.
Molecular Immunology

Microglial activation is an important contributor to neuroinflammation in intracerebral haemorrhage (ICH). IL-17A has been demonstrated to be involved in neuroinflammatory diseases such as multiple sclerosis. However, the exact mechanism of IL-17A mediated microglial activation in ICH has not been well identified. The purpose of this experiment is to investigate the role of IL-17A in ICH induced microglial activation and neuroinflammation. ICH mice were made by injection of autologous blood model. IL-17A expression and inflammatory factors in perihematomal region, and neurological function of mice were examined after ICH. In addition, IL-17A-neutralizing antibody was utilized to potentially prevent microglial activation and neuroinflammation in ICH mice. The expression of IL-17A, inflammatory factors and microglial activation in perihematomal region were significantly increased, and neurological function of mice was impaired after ICH. In addition, IL-17A Ab prevented ICH-induced cytokine expression, including TNF-α, IL-1β and IL-6, and downstream signaling molecules, including MyD88, TRIF, IκBα, and NF-κBp65 expression, and attenuated microglial activation. IL-17A Ab significantly reduced brain water content and improved neurological function of ICH mice. In conclusion, our results demonstrated that IL-17A was involved in ICH-induced microglial activation and neuroinflammation. IL-17A Ab might also provide a promising therapeutic strategy in ICH. © 2016 Elsevier Ltd. Source

Li J.,the First Affiliated Hospital of Zunyi Medical College | Wen K.-M.,the First Affiliated Hospital of Zunyi Medical College | Zeng Q.-L.,the First Affiliated Hospital of Zunyi Medical College
World Chinese Journal of Digestology

Octamer-binding transcription factor 4 (Oct4), a member of the POU transcription factor family, is one of the most important transcription factors for maintaining pluripotent and self-renewing state of stem cells. Oct4 is expressed not only in embryonic stem cells, germ cells and germ cell tumors but also in a variety of somatic cells of malignant tumors. The expression of Oct4 is closely related to the development and prognosis of malignant tumors. Therefore, detection of Oct4 expression has great significance in the diagnosis and treatment of tumors. This article provides a brief review of the role of Oct4 in gastrointestinal tumors. © 2013 Baishideng Publishing Group Co., Limited. All rights reserved. Source

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