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Zhang J.,the First Affiliated Hospital of Xinxiang Medical College
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2016

Objective: To investigate the effect of lentivirus-mediated bone morphogenetic protein (BMP-2) on the treatment of osteoporotic fractures. Methods: We divided 30 osteoporotic model rats into control group, model group and therapy group. The model group and therapy group were used to make the left tibial transverse fracture model. In the model group and the therapy group, rats were treated by local fracture subcutaneous injection of lentivirus which was packed with empty plasmid and BMP-2 plasmid. The rats in the control group were injected with PBS only. The three groups of rats were sacrificed at 8 weeks after operation, the expression of BMP-2 was used to analyze by Western blot. X-ray score, bone density, bone biomechanical index and bone structure were measured. Results: Lentivirus-mediated BMP-2 gene was expressed in vivo. Compared with that in the control group, the X-ray score in the model group and the therapy group was lower (P<0.05). The X-ray score was significantly higher in the therapy group than in model group (P<0.05). The bone mineral density in the model group and the therapy group was significantly reduced compared with that in the control group, while bone mineral density in the therapy group significantly increased compared with that in the model group (P<0.05). Compared with the control group, the 3 point bending parameters (maximum load, maximum stress, failure load and failure stress) in the model group and the therapy group significantly decreased, while the 3 point bending parameters in the therapy group were significantly higher than those in the model group (P<0.05). HE staining showed that there was cartilage callus and the trabecular bone was thin and sparse in the model group, while a large amount of cartilage callus changed into the bony callus in the therapy group. Conclusion: Lentivirus-mediated BMP-2 can promote bone formation and promote the healing of fracture site, which has a good therapeutic effect on osteoporotic fracture. © 2016, Editorial Board of Journal of Xi'an Jiaotong University (Medical Sciences). All right reserved.


Guo L.-K.,The First Affiliated Hospital of Xinxiang Medical College | Zhang C.-X.,The First Affiliated Hospital of Xinxiang Medical College | Guo X.-F.,The Sixth Peoples Hospital of Hangzhou
Journal of Chinese Integrative Medicine | Year: 2011

Background: Functional constipation is one of the most common gastrointestinal diseases. Currently, there is no effective Western medical therapy for functional constipation and it significantly impacts the quality of life of the patients. Integrated traditional Chinese and Western medicine therapies were reported to have better therapeutic effects than routine Western medicine therapies. Objective: To explore the efficacy of acupuncture combined with Chinese herbal medicine Plantain and Senna Granule in the treatment of functional constipation. Design, setting, participants and interventions: A total of 390 patients with confirmed functional constipation enrolled from the Department of Gastroenterology, the First Affiliated Hospital of Xinxiang Medical College in China from February 2008 to January 2010 were randomly divided into three groups, with 130 cases in each group. Acupuncture group was treated with puncture of point Tianshu (ST25), Shangjuxu (ST37), Zusanli (ST36), Dachangyu (BL25) and Zhigou (SJ6) twice daily for four weeks, while Plantain and Senna Granule group was treated with 5 g of Plantain and Senna Granule once daily, and the combination group was given above-mentioned acupuncture and Plantain and Senna Granule. Main outcome measures: The defecation cycle, stool property, constipation symptom, accompanying symptoms, gastrointestinal transit time, including total gastrointestinal transit time, mouth-intestine transit time, colonic transit time, right colonic transit time, left colonic transit time and rectosigmoid colonic transit time, and adverse reactions of the three groups were evaluated before treatment, at the end of treatment and 64 weeks after treatment, respectively. Results: Compared with before treatment, the scores of defecation cycle, stool property, constipation symptoms and accompanying symptoms, and gastrointestinal transit time all decreased significantly at the end of treatment in each group (P<0. 01), and the combination group showed better results than the other groups (P<0.05). Compared with the end of treatment, the above-mentioned indexes recurred insignificantly in the combination group and acupuncture group after 64 weeks of follow-up (P>0. 05), but all recurred significantly in the Plantain and Senna Granule group (P>0. 05). Both short- and long-term total response rates of the combination group showed significant differences compared with those of the other two groups (P<0. 05 or P<0.01). No serious adverse reactions occurred in all the three groups. Conclusion; Acupuncture plus Plantain and Senna Granule can significantly decrease gastrointestinal transit time and defecation cycle in patients with functional constipation; it can also change stool property and alleviate constipation symptom and accompanying symptoms, with good security and tolerance.


Guo L.K.,The First Affiliated Hospital of Xinxiang Medical College
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2011

To observe the long-term efficacy and safety on functional dyspepsia treated with electroacupuncture and Zhizhu Kuanzhong capsule and explore the therapeutic mechanism. Three hundreds and twenty patients with confirmed diagnosis as functional dyspepsia were randomly divided into 4 groups: an electroacupuncture group, a Zhizhu Kuanzhong capsule group, a combined therapy group and a western medication group, 80 cases in each group. In electroacupuncture group, acupuncture was applied to Zusanli (ST 36), Zhongwan (CV 12), Neiguan (PC 6), Taichong (LR 3) and Gongsun (SP 4), twice per day, continuously for 5 days each week. In Zhizhu Kuanzhong capsule group, Zhizhu Kuanzhong capsule, 1.29 g was administered for oral application, three times each day. In combined therapy group, the therapeutic programs in the above-mentioned groups were applied in combination. In western medication group, Mosapride 5 mg, three times per day; Omeprazole 20 mg, twice per day and Amitriptyline 25 mg, twice per day were administered for oral application. Six weeks treatment was required in each group. The symptoms score, the Nepean Dyspepsia Symptom Index (NDSI), the Nepean Dyspepsia Life Quality Index (NDLQI), Medical Outcomes Study 36-Item Short Form Health Survey (SF-36), electrogastrography, plasma motilin, gastric emptying by B-sonography and adverse reactions were observed before and after treatment, as well as 4 weeks after treatment separately. Compared with those before treatment, the symptom score and NDSI decreased apparently after treatment (all P < 0.01), NDLQI, SF-36, electrogastrographic frequency, plasma motilin and gastric emptying were up-regulated apparently (all P < 0.01) after treatment in each group. The results of the above-mentioned indices in combined therapy group were superior to the other groups (all P < 0.05). As compared with the condition at the end of treatment, in 4 weeks after treatment, the above indices were not rebound apparently in electroacupuncture group and combined therapy group (all P > 0.05). But they were rebound obviously in Zhizhu Kuanzhong capsule group and western medication group (all P < 0.05). The short-term and long-term efficacies in combined therapy group were significantly higher than the total effective rates in the other groups (P < 0.05, P < 0.01). No serious adverse reaction was reported in each group. Electroacupuncture and Zhizhu Kuanzhong capsule all up-regulate plasma motilin and electrogastrographic frequency, promote gastric emptying, alleviate the symptom of functional dyspepsia and improve NDLQI. But the combined medication achieves much better efficacy and presents better safety and long-term therapeutic effect.


Zhou G.Y.,The First Affiliated Hospital of Xinxiang Medical College
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2012

To test the therapeutic effect on moderate and severe sudden deafness treated with low-energy laser irradiation on acupoint and external auditory canal combined with auricular point sticking (APS) and as compared with electroacupuncture. Two hundred and fifty-eight cases of moderate and severe sudden hearing loss were randomly divided into an observation group 1, an observation group 2 and a control group, 86 cases in each group. In three groups, 10% low molecular Dextran 500 mL were used for intravenous infusion. Based on the above treatment, the observation group 1 was treated with low-energy laser irradiation on acupoint and external auditory canal (such as Ermen (TE 21), Tinggong (SI 19) and Tinghui (GB 2)), combined with APS at Gan (liver), Shen (kidney) and Neifenmi (endorine), etc. The observation group 2 was treated with electroacupuncture at the same acupoints as those point irradiation in observation group 1. Fifteen days made one session. The therapeutic effects were evaluated after one and two sessions. After two sessions, The cured rate was 40.7% (35/86) in observation group 1 and 38.4% (33/86) in observation group 2,which were superior to 25.6% (22/86) in control group (both P < 0.05). Compared with one session, the therapeutic effects after two sessions were better in two observation groups (both P < 0.05), but there was no significant difference between two groups (both P > 0.05). In comparison of the improvements of frequency audiometry and auditory function, the two observation groups were better than those in control group (P < 0.05, P < 0.01), and the improvements after two sessions were better in two observation groups (both P < 0.01). Both of low-energy laser irradiation on acupoint and external auditory canal combined with APS and electroacupuncture are able to decrease frequency audiometry, improve auditory function, and the therapeutic effects are better with prolongation of treatment time. The clinical efficacy of above two the rapies on moderate and severe sudden deafness is superior remarkably to that of conventional treatment. The therapy of low-energy laser irradiation on acupoint and external auditory canal combined with APS can replace the electroacupuncture therapy in treating moderate and severe sudden deafness.


Dong Y.Z.,The First Affiliated Hospital of Xinxiang Medical College | Huang Y.X.,The First Affiliated Hospital of Xinxiang Medical College | Lu T.,The First Affiliated Hospital of Xinxiang Medical College
Genetics and Molecular Research | Year: 2015

In this study, we investigated the association between a RECQL genetic polymorphism and osteosarcoma in a Chinese population. We selected rs820196 in the RECQL5 gene and genotyped 185 patients with osteosarcoma and 201 age- and gender-matched non-cancer controls. We found that the CC genotype was more frequent in the osteosarcoma group compared to the control group (P = 0.011). We also found that the C allele was more common in osteosarcoma patients than that in control subjects (P = 0.004). Our results suggested that the RECQL5 genetic polymorphism was associated with osteosarcoma in a Chinese population. ©FUNPEC-RP


Zhang C.X.,the First Affiliated Hospital of Xinxiang Medical College
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2012

To observe the clinical efficacy and safety of electroacupuncture combined with Dalitong granule for gastroesophageal reflux disease and to explore the therapeutic mechanism. Five hundred cases diagnosed as gastroesophageal reflux disease were randomly divided into a combination group, an electroacupuncture group, a Dalitong granule group, and a western medication group, 125 cases in each group. The electroacupuncture group was treated with acupuncture at Zusanli (ST 36), Zhongwan (CV 12), Neiguan (PC 6), Tai-chong (LR 3) and Gongsun (SP 4), once daily for 6 weeks; the Dalitong granule group was treated with oral administration of Dalitong granule 6 g, three times daily; the combination group was treated with above two methods; the western medication group was treated with oral administration of Mosapride 5 mg, three times daily, Omeprazole 20 mg, twice daily and Amitriptyline 25 mg, twice daily. The total refluxing times, times of long-term reflux, percentage of upright time, percentage of supine time, percentage of total time of the 24-hour intraesophageal pH < 4 or bilirubin absorbance value (Abs) > or = 0.14, symptom score, endoscopic score, life quality score and adverse reaction were observed before treatment, at the end of treatment and 48 weeks after treatment in four groups. Compared with those before treatment, esophageal acid reflux, bile reflux, endoscopic score and symptom score were decreased significantly at the end of treatment in four groups (all P < 0.01), while score of life quality was increased significantly (all P < 0.01). The improvements of above indices in the combination group were superior to other groups (all P < 0.05). Compared with the end of treatment, changes of above indices were not obvious in both of combination group and electroacupuncture group 48 weeks after treatment (all P > 0.05), but these indices all recurred significantly in other two groups (all P < 0.5). The short and long-term total effective rates in the combination group were superior to other groups (P < 0.5, P < 0.1). No serious adverse reaction occurred in four groups. Electroacupuncture and Dalitong granule can both inhibit esophageal acid reflux and bile reflux, decrease endoscopic score, alleviate the symptom of gastroesophageal reflux and improve life quality, but the effect of combination is much better with safety and long-term efficacy, which is correlated with their acid inhibition, gastrointestinal motility and antidepressant effects.


Xu D.,Traditional Chinese Medicine Hospital of Henan Province | Yang L.,the First Affiliated Hospital of Xinxiang Medical College | Li Y.,Traditional Chinese Medicine Hospital of Henan Province | Sun Y.,Traditional Chinese Medicine Hospital of Henan Province
Pakistan journal of pharmaceutical sciences | Year: 2015

This paper aims to make an analysis of the effects of ganglioside (GM) combined with methylprednisolone (MP) in early acute spinal injury. Fifty-three patients with acute spinal cord injury were included in this study and they were randomly divided into experimental and control group. Twenty-seven patients in the control group were treated with MP, while the rest 26 patients received more GM based on that. By observing and comparing the clinical responses from patients and recovery time of all indexes, results came out: the curative rates in the experimental and control group were 50.0%, 40.7% respectively, and the total effective rates were 92.3%, 85.2% respectively. There was a remarkable difference between the two groups (P<0.05). Patients in the experimental group took 6.2 ± 1.9d to restore their sphincter function, 11.2 ± 2.8d to recover their muscle forces to over grade II, and 13.8 ± 3.9d to return general activity, while the patients in the other group clearly spent longer time on recovery, that were 12.1 ± 3.2, 19.2 ± 4.6 and 23.9 ± 5.6 respectively. The distinct difference between the two groups was of statistical significance (P<0.05). We conclude that GM has better curative effects than MP, for it is able to promote the recovery of nerve function for patients and greatly improve the prognosis.


Qin S.,The First Affiliated Hospital of Xinxiang Medical College | Zhu Y.,The First Affiliated Hospital of Xinxiang Medical College | Ai F.,The First Affiliated Hospital of Xinxiang Medical College | Li Y.,The First Affiliated Hospital of Xinxiang Medical College | And 3 more authors.
Neoplasma | Year: 2014

MicroRNA-191 (miR-191) is reported to be overexpressed in colorectal carcinoma (CRC), but the role of miR-191 in CRC progress remained unclear. This study demonstrated that High miR-191 expression was associated with clinical stage, lymph node metastasis, liver metastasis and depth of tumor invasion. Kaplan-Meier analysis indicated that patients with high miR-191 expression had a poor overall survival. Moreover, multivariate analysis showed that miR-191 was an independent prognostic factor in patients with CRC. Furthermore, we found that tissue inhibitor of metalloprotease 3 (TIMP3) was a direct target of miR-191 in colorectal cancer SW620 cells. TIMP3 downregulation mediated by miR-191 activated matrix metalloproteinases (MMPs) and thus promoted invasiveness of cancer cells. Anti-miR-191 could attenuate the invasiveness, suppress proliferation and induce apoptosis by restoring TIMP3 expression. Our results suggested that miR-191 might be a potential diagnostic and therapeutic target in patients with colorectal cancer.


Zhang C.,the First Affiliated Hospital of Xinxiang Medical College | Guo L.,the First Affiliated Hospital of Xinxiang Medical College | Guo X.,the First Affiliated Hospital of Xinxiang Medical College
Wei sheng yan jiu = Journal of hygiene research | Year: 2012

OBJECTIVE: To investigate the correlation of the polymorphisms of EC-SOD and GSTM1, as well as smoking, with oral cancer risk.METHODS: A case-control study and polymerase chain reaction (PCR) technique was used to identify the genotype of EC-SOD and GSTM1 in 600 oral cancer cases and 600 controls. Logistic regression was conducted to evaluate the odds ratio of relative gene and the interaction between the genes and smoking with oral cancer.RESULTS: The frequencies of EC-SOD genotype in cases and controls were 61. 17% and 78.50% for C/C, and 38.33% and 21.50% for C/G respectively (P < 0.01). EC-SOD (C/G) correlated with susceptible to oral cancer (OR = 2.27, 95% CI 1.73 - 4.02). The frequency distribution of GSTM1 ( - ) was significantly different in patients (69.17%) and controls (44.17%) (P < 0.01). GSTM1 ( -) increased the risk of developing oral cancer (OR = 2.84, 95% CI 1.95 - 4.47). Combined polymorphism analysis showed that the percentage of EC-SOD (C/G)/GSTM1 (-) for oral cancer and control groups was 30.50% and 6.67% respectively) (P < 0.01). The risk of developing oral cancer in those with EC-SOD(C/G)/GSTM1 (-) genotype was significantly higher than that with EC-SOD (C/C)/GSTM1 (+) genotype (OR = 8.16, 95% CI 3.73 - 12.91). There were significant differences in smoking rate between the two groups (P < 0.01), smoking correlated with susceptible to oral cancer (OR = 2.66, 95% CI 1.45-4.36), and statistical analysis suggested an interaction between smoking and EC-SOD(C/G)/GSTM1(-) genotype, which increase the risk of oral cancer (OR = 25.11, 95% CI 12.37 - 36.62).CONCLUSION: EC-SOD (C/G) and GSTM1 (-) are risk factors in oral cancer. Smoking is also related to the susceptibility to oral cancer. There may be a synergetic interaction among EC-SOD (C/G), GSTM1 (-) and smoking on the elevated susceptibility of oral cancer.


Zhang Q.Y.,the First Affiliated Hospital of Xinxiang Medical College
Zhonghua nei ke za zhi [Chinese journal of internal medicine] | Year: 2011

To evaluate the efficacy and adverse effect of valproic acid (VPA) in combination with low dose chemotherapy on intermediate and high-risk myelodysplastic syndrome. A total of 41 patients with intermediate (34) and high-risk (7) myelodysplastic syndrome were retrospectively analyzed. Among them, 19 patients received low dose chemotherapy regimen and 22 received low dose chemotherapy plus VPA. Low dose chemotherapy regimen included: homoharringtonine, 1 - 2 mg×m(-2)×d(-1) intravenously, 14 - 28 d; aclarubicin, 5 - 7 mg×m(-2)×d(-1) intravenously, 1 - 8 d, 15 - 23 d; cytarabine 15 mg/m(2) subcutaneously once every 12 h, 14 - 21 d; and subcutaneously use of granulocyte colony-stimulating factor 200 μg·m(-2)×d(-1) when neutrophil deficiency. The outcome and adverse effect were recorded after the treatment. The overall response rate in the low dose chemotherapy regimen group was 47.4% (9/19), 6 patients (31.6%) achieved complete response (CR). The overall response rate in the VPA group was 77.2% (17/22), 9 patients (40.9%) achieved CR. The overall response rate of the low dose chemotherapy in combination with VPA group was significantly higher than that in the low dose chemotherapy group (P < 0.05) while no difference was found in CR rate. The adverse effect of the low dose chemotherapy in combination with VPA regimen was tolerated. With acceptable adverse effect, the low dose chemotherapy in combination with VPA regimen is effective for the treatment of intermediate and high-risk myelodysplastic syndrome. Long-term outcome needs further investigation.

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