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Bian L.,The First Affiliated Hospital of Kunming Medical College
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases

To study the expression and significance of fragile histidine triad (FHIT) and Ki-67 in transformed epithelial cells induced by Yunnan tin mine dust. Every second generation of immortalized human bronchial epithelial cells (BEAS-2B) and human embryo lung fibroblasts (WI-38) were exposed to 100 μg/ml Yunnan tin mine dust for 72 h, until the ninth generation. The cells were subsequently co-cultured from the 11th generation. Experimental setup: B group, B (W) group, B (W 100) group, B100 group, B100 (W) group, B100 (W100) group. The expressions of FHIT and Ki-67 in epithelial cells were determined by the method of immunocytochemistry at the 16th, 26th and 36th generation. The percentage of Ki-67 positive cells was calculated as proliferation index. The expression of FHIT was observed in BEAS-2B cells. The expression levels of FHIT among B group, B (W) group and B (W 100) group had not instinctive difference. At the 16th generation, the expression of FHIT in the B100 group was decreased compared with that in the B group and the expression of FHIT between B100 (W) group and B100 (W100) group was lower than that in the B100 group. At the 26th generation, the expression of FHIT was decreased compared with that at the 16th generation in the B100, B100 (W) and B100 (W100) groups. However, At the 36th generation, positive expression were observed again in the B100, B100 (W) and B100 (W100) groups and the expression levels were in incremental order. At the 16th, 26th and 36th generation, the proliferation indexes of B group, B (W) group and B (W 100) group were all < 3%. The proliferation indexes of B100, B100 (W) and B100 (W100) were increased step by step with the generation elongation. FHIT could be a target at which Yunnan tin mine dust induces transformation of BEAS-2B cells. The proliferation activation of BEAS-2B cells can be improved by Yunnan tin mine dust. Source

Li H.,Nanjing Southeast University | Fu W.-P.,The First Affiliated Hospital of Kunming Medical College | Hong Z.-H.,Nanjing Southeast University
Infection, Genetics and Evolution

C-type lectin domain family 4, member M (CLEC4M, also known as DC-SIGNR) is a C-type lectin that functions as a transreceptor for human immunodeficiency virus-1 (HIV-1). The relationship between variable number tandem repeat (VNTR) polymorphism of the DC-SIGNR gene and susceptibility to HIV-1 infection has been under debate. In the present study, a cohort of 287 HIV-1 seropositive patients and 388 ethnically age-matched healthy controls from Han Chinese population were enrolled in order to determine the influence of host genetic factors on HIV-1 infection. A total of 11 genotypes and 5 alleles were found in our population. A cross-sectional comparison between HIV-1 seropostive patients and healthy controls did not reveal significant differences with regards to DC-SIGNR genotype distribution, allele frequencies and homozygotes proportion. In addition, previous studies showed that DC-SIGNR might play different roles in different HIV infection routes. We stratified the patients into two subgroups: sexual contact patients and intravenous drug abuser/blood transfusion patients. Our results showed the frequencies of DC-SIGNR genotypes/alleles in these two subgroups were similar. To our knowledge, this is the first study performed in Northern Chinese. Our findings suggested that DC-SIGNR neck region VNTR polymorphism was not directly associated with hosts' predisposition for HIV-1 infection and not associated with the HIV-1 routes of infection. By lack of HIV-1 exposed seronegative (HESN) individuals and relative small sample size in present study made our conclusions not strong enough. In addition, the role of the DC-DIGNR neck region in different HIV-1 infection routes remains open for future study. © 2013 Elsevier B.V. Source

Zhong H.,Sun Yat Sen University | Zhong H.,The First Affiliated Hospital of Kunming Medical College | Sun G.,Sun Yat Sen University | Lin X.,Sun Yat Sen University | And 2 more authors.
Investigative Ophthalmology and Visual Science

Purpose. To investigate whether topical administration of pirfenidone eye drops could be used to prevent postoperative scarring in a rabbit model of experimental glaucoma filtration surgery. Methods. In a randomized, controlled, masked-observer study, 40 rabbits underwent trabeculectomy in the right eyes and randomly received postoperative administration of 0.1% or 0.5% pirfenidone, perioperative mitomycin C (0.25 mg/mL), or no treatment. Bleb characteristics and functions were evaluated over a period of 4 weeks. The animals were killed on days 7, 14, and 28. Histopathology and immunohistochemistry were performed to determine the amount of scarring and fibrosis. Ocular toxicity was assessed by the Draize test, histopathology, and electron microscope. Results. The four treatment groups were similar with respect to intraocular pressure and anterior chamber depth. Pirfenidone 0.5% significantly prolonged bleb survival, and the blebs were larger and higher than those in the control group (P < 0.05); the 0.1% pirfenidone concentration was less effective. Furthermore, the histology and immunohistology results showed that the 0.5% pirfenidone and mitomycin C groups had less scarring at days 7 to 28 than did the controls. Toxicity assessments showed that pirfenidone did not damage the rabbit eyes. Conclusions. Postoperative use of 0.5% pirfenidone eye drops was associated with improved trabeculectomy bleb survival in a rabbit model. Pirfenidone eye drops may be a safe and effective antiscarring agent in glaucoma filtration surgery. © 2011 The Association for Research in Vision and Ophthalmology, Inc. Source

Gu H.,Chinese University of Hong Kong | Gu H.,The First Affiliated Hospital of Kunming Medical College | Huang L.,Chinese University of Hong Kong | Wong Y.-P.,Chinese University of Hong Kong | Burd A.,Chinese University of Hong Kong
Experimental Dermatology

Hyaluronan (HA) is a major extracellular matrix component of the skin. Amongst its biological functions is the maintenance of epidermal homeostasis. The mechanisms of action, however, remain unclear. To explore the interaction of HA with the epidermis, we have looked at the effects of exogenous application of HA in an organotypic culture model containing a dermal substrate with and without fibroblast incorporation. The results demonstrate that exogenous HA enhances epidermal proliferation resulting in a thicker viable epidermis with an increase in the number and intensity of Ki67-positive basal cells; HA also improves the basement membrane assembly as evidenced by an increased expression of laminin-332 and collagen type IV but not the expression of Nidogen-1 at the epidermal-dermal junctional zone; furthermore, the development of epidermal lipid barrier structure was enhanced. These findings provide evidence to support the therapeutic use of exogenous HA for treating skin disorders with aberrant epidermal homeostasis. © 2010 John Wiley & Sons A/S. Source

Li H.,Nanjing Southeast University | Fu W.-P.,The First Affiliated Hospital of Kunming Medical College | Hong Z.-H.,Nanjing Southeast University
Infection, Genetics and Evolution

Mannose-binding lectin (MBL) plays an important role in immunity to HIV-1 infection. The exon1 coding polymorphisms of the MBL2 gene have been implicated in the susceptibility to HIV-1 infection, but the results were controversial. In the present study, a case-control study in a Chinese population was conducted to replicate the association, and then a meta-analysis combing our new data and published data was performed to clarify these findings. In total, 15 studies consisting 2219 HIV-1 patients and 2744 controls were included. Odds ratios (ORs) with 95% confidence intervals (95% CIs) were assessed in the main analyses. By dividing the controls into two groups, healthy controls and HIV-1 exposed but seronegative (HESN) controls, we explored different genetic models and allelic model to detect the association. By using the healthy controls, we found that the MBL2 exon 1 polymorphisms were associated with hosts' susceptibility to HIV-1 infection in dominant model (p=0.01, 95% CI 1.05-1.43), recessive model (p<0.0001, 95% CI 1.35-2.28), allelic model (p<0.0001, 95% CI 1.12-1.37) and O/O vs. A/A model (p<0.00001, 95% CI 1.40-2.38). In the subgroup analysis by ethnicity, significant elevated risks were found in Caucasians (recessive model: p<. 0.0001, 95% CI 1.36-2.51), but not in Asians (recessive model: p=0.10, 95% CI 0.91-2.77). Collectively, our findings from our case-control replication study and meta-analysis suggested that the MBL2 gene exon 1 coding variants were associated with hosts' susceptibility to HIV-1 infection, especially in Caucasians, but not in Asians. © 2013. Source

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