The First Affiliated Hospital of China Medical University

of China, China

The First Affiliated Hospital of China Medical University

of China, China
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News Article | May 18, 2017

Three years ago, results of a comprehensive study of dry eye syndrome in China were released and water advocate Sharon Kleyne paid attention. A 2014 major systematic review and meta-analysis conducted by The Department of Ophthalmology and the Department of Cardiovascular Ultrasound, both at The First Affiliated Hospital of China Medical University suggest that DES (Dry Eye Syndrome) increases with age. For instance, the Shihpai Eye Study found that 33.7% of people aged 65 in Taiwan were symptomatic. Women were shown to be especially susceptible to DES by a ratio of 64 to 1 (64 women for every man). People with Type 2 diabetes were also more likely to suffer from DES. Other risk factors for DES include alcohol, smoking, allergies, contact lens wear, computer use and ocular and systemic medications. The study also highlighted regional differences. Western China and Northern China had many more cases of DES than Central, Eastern and Southern China. Harkening back to an earlier study which showed that there is a strong link between ultraviolet radiation and DES, the current study suggests that Qinghai-Tibetan Plateau of Western China has high altitudes, long hours of sunlight and strong ultraviolet radiation that contributes to DES. Kleyne, founder of Bio-Logic Aqua® Research Water Life Science® and host of the nationally syndicated radio program, The Sharon Kleyne Hour Power of Water, Global Climate Change and Your Health on VoiceAmerica sponsored by Nature’s Tears® EyeMist®, likes tom put part of the focus of her radio show on this situation and she recently talked on the air about the prevalence of dry eye syndrome and its risk factors in Mainland China. Kleyne pointed to the disturbing conclusion three years ago that 17% of the Chinese population suffers from DES while today the number is closer to 25%. “Dry eye in China is out of control,” Kleyne said. “These are disturbing findings,” said Kleyne, “but relief is on the way.” John D. Ng, MD, MS, FACS, Chief—Ocular Plastics, Orbital and Reconstructive Surgery and a professor at Oregon Health & Science University is certain that he’s found the best solution available in today’s marketplace. “Nature’s Tears® EyeMist®,” said Ng, “has been an invaluable asset in rehabilitation of patients. I have been recommending this treatment for over 17 years and can attest to its benefit, its ease of use and lack of side effects as it is pure water without preservatives.” This product, developed and distributed by Kleyne and her research center at Bio-Logic Aqua® Research Water Life Science® is about to be released in Mainland China. “Nature’s Tears® EyeMist® is applied with a personal hand-held humidifying device,” said Kleyne. “The device emits a pure, pH balanced, 100% Trade Secret tissue culture grade water in a patented micron-size mist. It supplements the eye’s tear film. “With Natures Tears® EyeMist®,” Kleyne said, “Deeply abused and irritated eyes are supplemented with pure water and that’s what dry eyes need. Eye drops provide temporary chemical relief,” Kleyne continued, “eye drops can become addictive and even make the dry eye condition worse.” Why? Because eye drops only trap water on the eye’s tear film; eye drops do not supplement the tear film or the evaporation of water (moisture) of the eyelids.” Nature’s Tears® EyeMist® does supplement both.” Dry eye disease, Kleyne explained, is a major evaporation of water deficiency of the tear film disorder. Dry eye causes discomfort, vision impairment (red eye, blurred vision, stinging) and instability of the tear film due to excess evaporation of the eye’s water vapor. The eye must be supplemented daily with pure water to maintain a healthy water life science® lifestyle. Tear film evaporation and instability are especially dangerous for they can lead to damage of the ocular surface. In many cases in which dry eye is not addressed, the end result is blindness.

Wang Y.,The First Affiliated Hospital of China Medical University | Ma Y.,University College of Applied Sciences | Hu J.,Essential Qualities Oriented Education College | Zhang X.,The First Affiliated Hospital of China Medical University | And 8 more authors.
Experimental Neurology | Year: 2016

Both animal experiments and clinical studies have demonstrated that prenatal stress can cause cognitive disorders in offspring. To explore the scope of these deficits and identify potential underlying mechanisms, we examined the spatial learning and memory performance and glutamate receptor (GluR) expression patterns of adult rats exposed to prenatal chronic mild stress (PCMS). Principal component analysis (PCA) was employed to reveal the interrelationships among spatial learning indices and GluR expression changes. Female PCMS-exposed offspring exhibited markedly impaired spatial learning and memory in the Morris water maze (MWM) task compared to control females, while PCMS-exposed males showed better initial spatial learning in the MWM compared to control males. PCMS also altered basal and post-MWM glutamate receptor expression patterns, but these effects differed markedly between sexes. Male PCMS-exposed offspring exhibited elevated basal expression of NR1, mGluR5, and mGluR2/3 in the prefrontal cortex (PFC), whereas females showed no basal expression changes. Following MWM training, PCMS-exposed males expressed higher NR1 in the PFC and mammillary body (MB), higher mGluR2/3 in PFC, and lower NR2B in the hippocampus (HIP), PFC, and MB compared to unstressed MWM-trained males. Female PCMS-exposed offspring showed strongly reduced NR1 in MB and NR2B in the HIP, PFC, and MB, and increased mGluR2/3 in PFC compared to unstressed MWM-trained females. This is the first report suggesting that NMDA subunits in the MB are involved in spatial learning. Additionally, PCA further suggests that the NR1-NR2B form is the most important for spatial memory formation. These results reveal long-term sex-specific effects of PCMS on spatial learning and memory performance in adulthood and implicate GluR expression changes within HIP, PFC, and MB as possible molecular mechanisms underlying cognitive dysfunction in offspring exposed to prenatal stress. © 2016 Elsevier Inc.

Cao S.,China Medical University at Heping | Wang C.,China Medical University at Heping | Zheng Q.,China Medical University at Heping | Qiao Y.,Central University of Costa Rica | And 3 more authors.
Neuroscience Letters | Year: 2011

STAT5 activation in primary glioma was analyzed using antibody directed against phosphorylated STAT5 (pSTAT5), in all samples robust pSTAT5 immunostaining was detected, predominantly in the nucleus. We then used immunofluorescence, transcription factor binding assays and western blotting to study EGF-modulated STAT5 activation in the human U87 glioma cell line. EGF was found to upregulate pSTAT5 levels and enhances STAT5 DNA-binding activity. To address the role of STAT5 in glioma cell invasion, resting and EGF-treated U87 cells were treated with siRNA directed against STAT5 and the extent of glioma cell migration into a Matrigel matrix was monitored. EGF treatment markedly enhanced matrix invasion, and knockdown of STAT5 expression with siRNA significantly downregulated matrix invasion by both EGF-stimulated and resting glioma cells. However, transfection with a decoy oligonucleotide abolished transcriptional activation of a STAT5 target gene but failed to inhibit matrix invasion in resting U87 cells. By contrast, the decoy nucleotide abolished EGF enhancement of matrix invasion. STAT5 therefore promotes glioma cell invasion via at least two different pathways: a pathway dependent of STAT5 DNA binding, and a second pathway independent of STAT5 DNA binding. © 2010 Elsevier Ireland Ltd.

PubMed | The First Affiliated Hospital Of China Medical University
Type: | Journal: European journal of pharmacology | Year: 2016

Nowadays, drugs protecting ischemia/reperfusion (I/R) myocardium become more suitable for clinic. It has been confirmed lycopene has various protections, but lacking the observation of its effect on endoplasmic reticulum stress (ERS)-mediated apoptosis caused by hypoxia/reoxygenation (H/R). This study aims to clarify the protective effect of lycopene on ERS induced by H/R in H9C2 cardiomyocytes. Detect the survival rate, lactic dehydrogenase (LDH) activity, apoptosis ratio, glucose-regulated proteins 78 (GRP78), C/EBP homologous protein (CHOP), c-Jun-N-terminal protein Kinase (JNK) and Caspase-12 mRNA and protein expression and phosphorylation of JNK (p-JNK) protein expression. LDH activity, apoptosis ratio and GRP78 protein expression increase in the H/R group, reduced by lycopene. The survival rate reduces in the H/R and thapsigargin (TG) groups; lycopene and 4-phenyl butyric acid (4-PBA) can improve it caused by H/R, lycopene also can improve it caused by TG. The apoptosis ratio, the expression of GRP78, CHOP and Caspase-12 mRNA and protein and p-JNK protein increase in the H/R and TG groups, weaken in the lycopene+H/R, 4-PBA+H/R and lycopene+TG groups. There is no obvious change in the expression of JNK mRNA or protein. Hence, our results provide the evidence that 10 M lycopene plays an obviously protective effect on H/R H9C2 cardiomyocytes, realized through reducing ERS and apoptosis. The possible mechanism may be related to CHOP, p-JNK and Caspase-12 pathways.

Lv M.,The First Affiliated Hospital of China Medical University | Li B.,Liaoning Medical University | Li Y.,The First Affiliated Hospital of China Medical University | Mao X.,The First Affiliated Hospital of China Medical University | And 2 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2011

Introduction: Breast cancer is increasingly regarded as a heterogeneous disease which can be classified into distinct molecular subtypes with prognostic significance. Materials and methods: ER, PR, HER2 and ki-67 were used to divided 102 breast cancers treated with neoadjuvant chemotherapy (NCT) into 4 subtypes: luminal A (ER+, PR+, HER2-, and ki-67 ≤14%), luminal B (ER+, PR+, HER2- and ki-67>14%; ER+ and/or PR+, HER2+), HER2-overexpression (ER-, PR- and HER2+) and triple-negative (ER-, PR-,and HER2 Results: Among 102 patients, a pCR was seen in 16 (15.7%) patients. The pathologic complete remission (pC) rates according to different subtypes are as follows: luminal A, 0 of 20 (0.0%), luminal B, 2 of 23 (8.7%), HER2-overexpression 4 of 18 (22.2%), and triple-negative, 10 of 41 (24.4%) (p=0.041). In triple-negative subtype patients, the rates of pCR differed significantly among the 3 chemotherapy regimens with 5.6% (1/18) for CEF (cyclophosphamide, epirubicin and flurouracil), 20.0% (1/5) for TE (docetaxel and epirubicin) and 44.4% (8/18) for TCb (docetaxel and carboplatin) (p=0.024). In locally advanced breast cancer patients, the rates of pCR seem to differ among the 3 chemotherapy regimens with 6.7% (2/30) for CEF, 0.0% (0/8) for TE and 23.1% (6/26) for TCb, but this did not attain statistical significance (p>0.05). Conclusions: Molecular subtypes are good predictors for response to NCT in breast cancer patients in Northeast China. Compared with luminal A tumors, HER2-overexpression and triple-negative subtypes are more sensitive to NCT. For triple-negative breast cancer, we concluded that the TCb combination is a promising NCT regimen. Our results also indicated that the TCb combination is promising for the treatment of locally advanced breast cancer.

Shao C.,The First Affiliated Hospital of China Medical University | Gu J.,The First Affiliated Hospital of China Medical University | Meng X.,The First Affiliated Hospital of China Medical University | Zheng H.,The First Affiliated Hospital of China Medical University | Wang D.,The First Affiliated Hospital of China Medical University
International Journal of Clinical and Experimental Medicine | Year: 2015

Objectives: Glucose fluctuation is suggested to be the leading cause of beta-cell damages. To determine how it induces beta-cell dysfunction, we systematically evaluated the effects of intermittent high glucose (IHG) in INS-1 rat pancreatic beta-cells on their proliferation activity, apoptosis, insulin secretion, reactive oxygen species (ROS), intracellular concentration of Ca2+ ([Ca2+]i), and the PTEN expression as well as AKT phosphorylation. Methods: Prior to the examinations, INS-1 cells were treated with normal glucose (NG, 11.1 mmol/L), sustained high glucose (SHG, 33 mmol/L), IHG (switching per 12 h in 11.1 mmol/l or 33 mmol/L), NG+α-lipoic acid (LA, pretreated with LA 12 h before exposure to NG), SHG+LA (pretreated with LA 12 h before being exposed to 33.3 mmol/L glucose) and IHG+LA (pretreated with LA 12 h before being cultured with IHG). The cells in each group were cultured with indicated concentrations of glucose for 3 days. The evaluations were carried out on the cell viability, apoptosis rate, insulin secretion, [Ca2+]i, ROS and the expressions of PTEN and p-AKT. Results: The current study determined that IHG induces more apoptosis and significant increases of [Ca2+]i and intracellular ROS levels, compared to SHG and NG treatments to INS-1 cells. Moreover, IHG leads to more than 20% decrease on cell viability and over 50% reduction on insulin secretion (from 5.48±0.79 mIU/L to 2.51±0.58 mIU/L). The negative regulation of IHG on insulin signaling in beta-cells is identified via western blot analysis with results of the elevated expression of PTEN and lowered phosphorylation levels of AKT post IHG treatment. While the pretreatment of the antioxidant LA can significantly suppress the above responses induced by high glucose treatment. Conclusions: This study demonstrated that IHG plays a detrimental role in the viability, expansion, and function of beta-cells. IHG could be more harmful to the INS-1 cells than the SHG treatment. The rate increase of apoptosis in beta-cells could be caused by the suppressed insulin signaling, which is resulted from the raised ROS level by abnormal glucose treatments. Undergoing oxidative stress induced by high glucose treatments, including SHG and IHG, might be an important player in mediating the injury process to beta-cells, concluded from the beneficial rescue by the antioxidant LA treatment. © 2015, E-Century Publishing Corporation. All rights reserved.

Wang B.,The First Affiliated Hospital of China Medical University | Liu L.,The First Affiliated Hospital of China Medical University
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology | Year: 2016

Along with the aging process, the spectrum of liver disease changes greatly. Nonalcoholic fatty liver disease (NAFLD) in elderly people lead to low liver function and is also the major cause of extrahepatic diseases, such as cardiovascular disease and malignant tumor. This review provides an overview of the morphological structure and function of the liver in aged people, and discusses the characteristics of weakness, malnutrition and limited movement in the elderly, as well as the current status of multiple diseases and multiple drug use. Finally, this article puts forward some appropriate regimens for the diagnosis and treatment of NAFLD in elderly people to provide a reference for clinical practice.

Yin Y.,The First Affiliated Hospital of China Medical University | Hou G.,The First Affiliated Hospital of China Medical University | Li E.,The First Affiliated Hospital of China Medical University | Wang Q.,The First Affiliated Hospital of China Medical University | Kang J.,The First Affiliated Hospital of China Medical University
Respiratory Research | Year: 2014

Background: Peroxisome proliferator-activated receptor-gamma (PPARγ) is a ligand-activated transcription factor that exerts multiple biological effects. Growing evidence suggests that PPARγ plays an important role in inflammation; however, the effects of this transcription factor on the inflammation caused by smoking are unclear.Methods: We measured the expression of inflammatory cytokines (leukotriene B4, LTB4 and interleukin 8, IL-8), PPARγ and toll-like receptors (TLR2 and TLR4) in alveolar macrophages (AMs) harvested from rats exposed to cigarette smoke (CS) for 3 months in vivo. Some of the rats were pre-treated with rosiglitazone (PPARγ agonist, 3 mg/kg/day, ip), rosiglitazone (3 mg/kg/day, ip) + BADGE (bisphenol A diglycidyl ether, a PPARγ antagonist, 30 mg/kg/day, ig), or BADGE alone (30 mg/kg/day, ig). We also measured the expression of PPARγ, TLR2, TLR4 and nuclear factor-kappaB (NF-κB) in AMs gained from normal rats, which exposed to 5% CSE (cigarette smoke extract) for 12hrs, respectively pretreated with PBS, rosiglitazone (30 uM), rosiglitazone (30 uM) + BADGE (100 uM), 15d-PGJ2 (PPARγ agonist, 5 uM), 15d-PGJ2 (5 uM) + BADGE (100 uM), or BADGE (100 uM) alone for 30 min in vitro.Results: In vivo, rosiglitazone counteracted CS-induced LTB4 and IL-8 release and PPARγ downregulation, markedly lowering the expression of TLR4 and TLR2. In vitro, both rosiglitazone and 15d-PGJ2 inhibited CS-induced inflammation through the TLR4 signaling pathway.Conclusions: These results suggest that PPARγ agonists regulate inflammation in alveolar macrophages and may play a role in inflammatory diseases such as COPD. © 2014 Yin et al.; licensee BioMed Central Ltd.

PubMed | The First Affiliated Hospital of China Medical University and Dalian Medical University
Type: | Journal: Postgraduate medicine | Year: 2017

Immunoglobulin A nephropathy (IgAN) is the most frequent cause of primary renal disease, and clarifying the pathogenesis of IgAN is of great importance for its diagnosis and treatment. It is well known that Mycobacterium tuberculosis (MTB) can infect the urinary tract and result in the typical symptoms of cystitis. However, MTB can also affect the kidney more insidiously. Patients may present with glomerular disease, and sometimes with advanced renal failure. This study was to investigate the association between MTB infection and IgA nephropathy (IgAN), and the early diagnosis of MTB-mediated IgAN by means of early secreted antigenic target 6 (ESAT-6) detection in renal biopsies.One hundred and twenty patients were divided into 3 groups: a renal tuberculosis (RTB) group, a glomerulonephritis without MTB infection (GN-TBI) group and a glomerulonephritis with MTB infection (GN+TBI) group. Morning urine samples were collected for MTB culture. Immunohistochemistry for ESAT-6 expression in renal tissues was performed.The incidence rate of IgAN in the GN+TBI group was 66.7%, which was significantly higher than that of the GN-TBI group. In the GN+TBI group, the ESAT-6 expression was positively associated with IgAN incidence. There was a statistical association between the positive expression of ESAT-6 and the incidence of IgAN. The sensitivity and specificity of urine MTB culture in diagnosing renal MTB infection was 23.3% and 100% respectively, while the sensitivity and specificity of ESAT-6 detection was 100% and 91.1% respectively. Compared with urine MTB culture, the sensitivity of ESAT-6 detection was significantly increased.MTB infection might be associated with the occurrence of IgAN, and ESAT-6 detection in renal tissues may be helpful for the early diagnosis of MTB-mediated IgAN.

PubMed | The First Affiliated Hospital of China Medical University
Type: Journal Article | Journal: Pain physician | Year: 2016

Postherpetic neuralgia (PHN) is often refractory to existing treatments. Treatment of the dorsal root ganglion (DRG) using monopolar pulsed radiofrequency (PRF), which is a non- or minimally neurodestructive technique, is not efficacious in all patients.This study aimed to determine the safety and clinical efficacy of bipolar high-voltage, long-duration PRF on the DRG in PHN patients.Self before-after controlled clinical trial.Department of Pain Medicine, the First Affiliated Hospital of China Medical University.Ninety patients diagnosed with PHN for > 3months were included. Bipolar high-voltage, long-duration PRF at 42C for 900 seconds was applied after the induction of paresthesias covered the regions of hyperalgesic skin. The therapeutic effects were evaluated using a visual analog scale (VAS) and the 36-item Short Form health survey (SF-36) before treatment and one, 4, 8, and 12 weeks after PRF.The VAS scores at one, 4, 8, and 12 weeks after PRF treatment were significantly lower than before treatment (P < 0.001). The SF-36 scores, which included physical functioning, physical role, bodily pain, general health perceptions, vitality, social function, emotional role, and the mental health index, were significantly improved up to 12 weeks after PRF treatment (P < 0.001). No serious adverse effects were identified following treatment. The main adverse reactions included pain, tachycardia, and high blood pressure (especially when the field strength was enhanced).Single center study, relatively small number of patients, lack of a control group.Bipolar high-voltage, long-duration PRF on the DRG is an effective and safe therapeutic alternative for PHN patients. This treatment could improve the quality of life of PHN patients.NO ChiCTR-OCS-14005461.

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