Han Z.,The First Affiliated Hospital of Bengbu Medical CollegeAnhui |
Han X.,The First Affiliated Hospital of Bengbu Medical CollegeAnhui |
Wang Z.,The First Affiliated Hospital of Bengbu Medical CollegeAnhui |
Wu S.,Bengbu Medical College |
Zheng R.,The First Affiliated Hospital of Bengbu Medical CollegeAnhui
International Journal of Clinical and Experimental Medicine | Year: 2016
Objective: To prepare thioaptamer conjugated single-wall carbon nanotubes (SWNT-PEG-TA) and investigate their effect in human breast cancer targeted photothermal therapy in-vivo and in-vitro. Methods: An ultrasonic cell disruptor was used to prepare nanoscale single-wall carbon nanotubes (SWNT). SWNT-PEG with good water solubility was obtained by non-covalent modification of SWNT with polyethylene glycol (PEG). A thermoelectric thermometer was used to detect the temperature of SWNT-PEG-TA solution after irradiating with 808 nm laser of different energy. The condition of SWNT-PEG-TA after irradiating with 1 W/cm2 power density of laser for 5 minutes was detected by a near-infrared thermal imager. Cell counting kit (CCK-8) method was used to detect the effect of SWNT-PEG on cell viability, as well as the effect of SWNT-PEG and SWNT-PEG-TA on cell viability under 1 W/cm2 laser irradiation. SWNT-PEG and SWNT-PEG-TA were labeled with isothiocyanates dye (FITC) and were then incubated with cells, which were observed under a confocal microscope for cell uptake. In a subcutaneous breast cancer model in mice established, 24 hours after injecting SWNT-PEG and SWNT-PEG-TA through caudal vein, mice imaging was conducted by using a near-infrared thermal imager during 808 nm laser irradiation on the back. Then, the tumor volume was monitored. Results: The temperature of SWNT-PEG-TA solution after irradiating with 1 W/cm2 laser for 5 min reached up to 62.4±3.8°C. More SWNT-PEG-TA fluorescence was observed by confocal microscopy than SWNT-PEG. No significant effect on cell viability was found for cells treated by 0-10 μg/mL SWNT-PEG for 12 and 24 h. After irradiating by 1 W/cm2 laser for 5 min, the viability of cells treated by SWNT-PEG-TA decreased remarkably compared with those treated by SWNT-PEG. After injecting equal quantities of SWNT-PEG and SWNT-PEG-TA through caudal vein, the temperature in tumor region reached up to 44.9 and 63.1°C, respectively, under near-infrared thermal imaging. Further, evident tumor ablation was observed 30 days after the photothermal therapy with SWNTPEG-TA. By contrast, no significant inhibitory effect on tumor growth was found after the photothermal therapy with SWNT-PEG. Conclusion: SWNT-PEG-TA prepared in this study can target cell surface actively and effectively and has a good cell-killing effect under 808 nm laser radiation, playing a good anti-cancer effect in in-vivo tumor treatment. © 2016, E-Century Publishing Corporation. All rights reserved.