News Article | May 25, 2017
For humans, there are hundreds of antibodies available on the market today to evaluate immune status in health and diseases. However, for the more than 42 known species of dolphins around the world, commercially available marine-specific antibodies do not exist. With the drastic increase in the number of unusual dolphin strandings and deaths along the southeastern coast of the United States and elsewhere, finding specific antibodies to test, monitor and document their immune health is critical. Researchers from Florida Atlantic University's Charles E. Schmidt College of Medicine in collaboration with Georgia Aquarium, conducted a study to identify cross-reactive terrestrial-specific antibodies for dolphins, which is the first study to characterize their immune cell subsets using this methodology. The goal of this study was to assess changes in immune cell populations in dolphins in the wild and results are published in the journal BMC Veterinary Research. "We know that a strong immune system is important for combatting infectious diseases and cancer in both humans and animals," said Mahyar Nouri-Shirazi, DVM., Ph.D., lead author of the study and a professor of integrated medical science in FAU's College of Medicine. "Wild dolphins are impacted by newly characterized infectious disease and cancer, often associated with immunologic disturbances, which are now being better characterized." While there are a number of studies and reports that suggest a correlation between environmental contaminants, immune disturbances and disease susceptibility in wild dolphins and other marine mammals, scientists and veterinarians need a way to characterize, monitor and evaluate specific immune cells to investigate and confirm this correlation. The gold standard to monitor immune status and disease progression in humans is flow cytometry, a powerful tool that is used to rapidly measure and isolate immune cells. However, flow cytometry for dolphins requires the use of dolphin specific antibodies that are presently limited for the many different types of immune cells. To address this limitation, Nouri-Shirazi's team identified cross-reactive, terrestrial-specific antibodies to phenotype the immune cells of dolphins under human care and compare them with the immune status of Atlantic bottlenose dolphins in the wild. "When we compared the samples, we were able to see that the profile of some of the dolphins in the wild had changed significantly," said Nouri-Shirazi. "We saw abnormal distributions and increases in the percentages of the immune cells, which indirectly tells us that there may be a disease present." Results from this study show that out of 65 terrestrial-specific antibodies tested, 11 were cross-reactive and identified dolphin immune cell populations within their peripheral blood. Using these antibodies, the researchers found significant differences in the absolute number of cells expressing specific markers within their lymphocyte and monocyte fractions. They found that the cross-reactive antibodies not only identified specific changes in the immune cells of dolphins in the wild, but also opened up the possibility to investigate the causal relationship between immune disturbances and morbidity and mortality seen in these dolphins in the wild. "Georgia Aquarium's collaborative bottlenose dolphin research with FAU has demonstrated the intricate and dynamic interactions that occur between infectious disease, anthropogenic contaminants and immunologic responses. We have found that these interactions highlight the complexity of evaluating health in dolphins," said Gregory Bossart, VMD, Ph.D., co-author of the study and senior vice president and chief veterinary officer, Georgia aquarium. "Additionally, we have demonstrated that the bottlenose dolphin is a valuable sentinel species for understanding emerging or re-emerging diseases that may impact environmental and human health." Findings from this study open up the possibility of utilizing flow cytometry for routine health assessment by monitoring specific changes in immune cells of wild dolphins caused by environmental contaminants or infectious agents with the goal of understanding pathogenesis of diseases. The researchers anticipate that this new approach could be applicable to dolphins as well as other marine mammals including whales and manatees. "Down the road, we hope to further develop this approach as an important tool to accurately gauge dolphin health and immunity," said Nouri-Shirazi. "Ideally, we hope that our research triggers interest for companies to further develop and commercialize these biomarkers to help us monitor and document dolphin health worldwide." Co-authors of "Phenotyping and Comparing the Immune Cell Populations of Free-ranging Atlantic Bottlenose Dolphins (Tursiops truncates) and Dolphins Under Human Care" are Brittany F. Bible, FAU; Menghua Zeng, The First Affiliated Hospital of Chongqing Medical Center; and Saba Tamjidi, FAU. This research was sponsored by Georgia Aquarium's Research and Conservation Program. About the Charles E. Schmidt College of Medicine FAU's Charles E. Schmidt College of Medicine is one of 147 accredited medical schools in the U.S. The college was launched in 2010, when the Florida Board of Governors made a landmark decision authorizing FAU to award the M.D. degree. After receiving approval from the Florida legislature and the governor, it became the 134th allopathic medical school in North America. With more than 70 full and part-time faculty and more than 1,300 affiliate faculty, the college matriculates 64 medical students each year and has been nationally recognized for its innovative curriculum. To further FAU's commitment to increase much needed medical residency positions in Palm Beach County and to ensure that the region will continue to have an adequate and well-trained physician workforce, the FAU Charles E. Schmidt College of Medicine Consortium for Graduate Medical Education (GME) was formed in fall 2011 with five leading hospitals in Palm Beach County. In June 2014, FAU's College of Medicine welcomed its inaugural class of 36 residents in its first University-sponsored residency in internal medicine. Florida Atlantic University, established in 1961, officially opened its doors in 1964 as the fifth public university in Florida. Today, the University, with an annual economic impact of $6.3 billion, serves more than 30,000 undergraduate and graduate students at sites throughout its six-county service region in southeast Florida. FAU's world-class teaching and research faculty serves students through 10 colleges: the Dorothy F. Schmidt College of Arts and Letters, the College of Business, the College for Design and Social Inquiry, the College of Education, the College of Engineering and Computer Science, the Graduate College, the Harriet L. Wilkes Honors College, the Charles E. Schmidt College of Medicine, the Christine E. Lynn College of Nursing and the Charles E. Schmidt College of Science. FAU is ranked as a High Research Activity institution by the Carnegie Foundation for the Advancement of Teaching. The University is placing special focus on the rapid development of critical areas that form the basis of its strategic plan: Healthy aging, biotech, coastal and marine issues, neuroscience, regenerative medicine, informatics, lifespan and the environment. These areas provide opportunities for faculty and students to build upon FAU's existing strengths in research and scholarship. For more information, visit http://www. . Georgia Aquarium is a leading 501(c)(3) non-profit organization located in Atlanta, Ga. that is Humane Certified by American Humane and accredited by the Alliance of Marine Mammal Parks and Aquariums and the Association of Zoos and Aquariums. Georgia Aquarium is committed to working on behalf of all marine life through education, preservation, exceptional animal care, and research across the globe. Georgia Aquarium continues its mission each day to inspire, educate, and entertain its millions of guests about the aquatic biodiversity throughout the world through its hundreds of exhibits and tens of thousands of animals across its seven major galleries. For more information, visit georgiaaquarium.org.
Yang L.,the First Affiliated Hospital |
Sun X.,the First Affiliated Hospital |
Geng X.,the First Affiliated Hospital
Pakistan journal of pharmaceutical sciences | Year: 2015
Discuss the internal mechanism of delaying degeneration of lumber intervertebral disc. The cartilage of lumbar intervertebral disc of SD rats was selected in vitro, then cultured by tissue explant method, and identified by HE staining, toluidine blue staining and immunofluorescence. The optimal concentration of psoralen was screened by cell proliferation assay and RT-PCR method. The cells in third generation with good growth situation is selected and placed in 6-well plate at concentration of 1×10(5)/well and its expression was tested. Compared to concentration of 0, the mRNA expression of Col2al (Collagen Ⅱ) secreted by was up regulated chondrocyte of lumbar intervertebral disc at the concentration of 12.5 and 25μM (P<0.0 or P<0.01). The aggrecan mRNA of psoralen group was higher than blank control group (P<0.01); compared with IL-1β induced group, the mRNA expression of Col2al was significantly increased but the mRNA expression of ADAMTS-5 was significantly decreased in psoralen group (P<0.01). These findings suggest that, psoralen can remit the degeneration of lumbar intervertebral disc induced by IL-1β to some extent, and affect the related factors of IL-1β signaling pathway.
Tang K.F.,The First Affiliated Hospital
Zhonghua nan ke xue = National journal of andrology | Year: 2011
To investigate the effects of tamoxifen (TMX) combined with coenzyme Q10 (CoQ10) on idiopathic oligoasthenospermia. A total of 183 patients with idiopathic oligoasthenospermia were randomly divided into a TMX + CoQ10 group (n = 63), a TMX group (n = 61) and a CoQ10 group (n = 59). At the end of 3 and 6 months of treatment, semen analyses and hormone tests were performed, and the results were compared with those obtained before the treatment. Compared with the pre-treatment results, the levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone (T) and sperm concentration were significantly elevated in the TMX + CoQ10 and TMX groups (P < 0.05), but showed no significant difference in the CoQ10 group (P > 0.05); sperm motility and morphologically normal sperm were increased significantly in the TMX + CoQ10 and CoQ10 groups (P < 0.05), and slightly in the TMX group but with no statistically significant difference (P > 0.05). Tamoxifen combined with CoQ10 can significantly improve sperm concentration, motility and morphology in patients with idiopathic oligoasthenospermia.
Wang X.M.,The First Affiliated Hospital
Zhonghua nan ke xue = National journal of andrology | Year: 2012
To study the effects of dihydroartemisinin on the apoptosis of and the vascular endothelial growth factor (VEGF) expression in prostate cancer cell line PC-3M in androgen-independent prostate cancer. PC-3M cells were treated with different doses (0, 25, 50 and 100 micromol/L) of dihydroartemisinin for 48 hours, their growth activity analyzed by MTT colorimetric assay and flow cytometry, and changes in the activities of caspase-3 and -8 detected by colorimetric assay. The expression of VEGF mRNA was determined by semi-quantitative RT-PCR, and that of the VEGF protein by Western blotting. Compared with the 0 micromol/L control group, the 25, 50 and 100 micromol/L dihydroartemisinin groups showed significantly increased apoptosis of PC-3M cells ([2.92 +/- 0.45]% vs [8.85 +/- 0.74]%, [12.83 +/- 0.84]% and [18.65 +/- 1.24]%, P < 0.01), and dose-dependent increase in the activities of caspase-8 ([0.47 +/- 0.05 ] U/microg vs [1.22 +/- 0.15], [1.76 +/- 0.07] and [2.91 +/- 0.24] U/microg, P < 0.01) and caspase-3 ([0.44 +/- 0.07] U/microg vs [0.95 +/- 0.08], [1.48 +/- 0.14] and [2.92 +/- 0.45] U/microg, P < 0.01). The expressions of VEGF mRNA and protein were decreased in a concentration-dependent manner. Dihydroartemisinin can significantly suppress the growth of PC-3M cells, promote their apoptosis and reduce the expressions of VEGF mRNA and protein, which may serve to explain its inhibitory effect on tumor and angiogenesis.
Li H.B.,The First Affiliated Hospital
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2011
To study the effects of berberine on hypertensive renal injury model in rats fed by enriched high fat-salt-fructose diet . hypertensive renal injury model was esteblished by feeding enriched high fat-salt-fructose diet for 8 weeks. On the basis of animal blood pressure, hypertensive rats were randomly divided into model group (10 rats, distilled water), captopril group (10 rats,25 mg/kg), berberine high dose group (10 rats, 300 mg/kg) and low dose group (10 rats, 100 mg/kg). These rats were fed by enriched high fat-salt-fructose diet and treated by intragastric administration with drugs for 4 weeks. And normal control group (10 rats) was set Blood pressure was determined at 0, 4, 8, 10, 12 weekend,and after 4 weeks of drugs treatment, getting urine to determine urine protein, taking blood serum to determine blood urea nitrogen, serum creatinine,GHb,MDA and activity of SOD. The content of H2O2 and GSH-Px and activity of CAT in kidney tissues were determined also. Compared with normal control group, blood pressure, urine protein, blood urea nitrogen, serum creatinine and MDA and GHb in serum of model rats obviously increased (P < 0.01), the activity of SOD decreased (P < 0.01), higher content of H2O2 and lower content of GSH-Px and activity of CAT (P < 0.01) in the kidney tissues. Treated with berberine for 4 weeks, elevated blood pressure and heightened levels of urine protein, blood urea nitrogen and serum creatinine in model rats were depressed significantly (P < 0.01), and elevated the activity of SOD, lowed the levels of MDA and GHb in blood serum (P < 0.01). At the same time, berberine increased the activities of GSH-Px and CAT (P < 0.01) and slightly lowed the content of H2O2 in the kidney tissues. Berberine has protecting effects on the hypertensive renal impairment model rats fed by enriched high fat-salt-fructose diet, which are concerned with elevated antioxidant capability in body and kidney tissues.
Li C.,The First Affiliated Hospital
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery | Year: 2011
To investigate the relationship between methylation of the CDH1 gene promoter on the expression of E-cadherin and β-catenin, and to evaluate the correlation with clinicopathological characteristics of the colonic carcinoma. Methylation specific PCR (MSP) was used to detect CDH1 gene promoter methylation in the cancer tissue, adjacent tissues and normal tissues in 68 patients. The expression of E-cadherin and β-catenin was determined by immunohistochemistry staining. The positive rate of CDH1 gene promoter methylation was 32.4% in adjacent tissues and 57.4% in cancer tissue, while no detectable methylation was found in all the normal tissues. The difference was statistically significant. The positive rate of E-cadherin was 92.6% in the normal tissues, 66.2% in the adjacent tissues and 44.1% in the cancer tissues. In all normal tissues, β-catenin was expressed only at the cellular membrane but not in the cytosol or nucleus, while the expression of β-catenin was present in the cytosol or nucleus in 29.4% of the adjacent tissues and 50.0% of the cancer tissues. The positive rate of CDH1 gene promoter methylation was negatively correlated with E-cadherin expression(r=-0.312, P=0.01) and positively correlated with β-catenin cytosolic/nucleus expression(r=0.309, P=0.018). The differentiation and metastasis of colonic carcinoma were associated with the aberrant expression of E-cadherin, β-catenin, and methylation of CDH1 promoter (P<0.05). CDH1 gene promoter methylation may lead to aberrant expression of E-cadherin and β-catenin in colonic carcinoma, and may play an important role in promoting the invasion of tumor.
Sui R.,The First Affiliated Hospital |
Zhang L.,Liaoning Medical College
Neurological Research | Year: 2011
Objective: To explore the risk factors for stroke-associated pneumonia (SAP). Methods: A retrospective research study was carried out to investigate the clinical data of 1435 patients admitted to the neurological intensive care unit at our university hospital between 1 January 2000 and 31 December 2009. Results: A multi-factorial analysis produced the following results: (1) SAP is 1.113 times more likely to occur for each 1-year increase in age; (2) diabetic patients are 1.612 times more likely to develop SAP than nondiabetic patients; (3) the incidence of SAP decreases by a factor of 0.890 with a one-point increase in the Glasgow coma scale score; (4) nasal feeding patients are 4.981 times more likely to develop SAP than nonnasal feeding patients; (5) patients who use H2-receptor blocking agents are 2.837 times more likely to develop SAP than those who do not; (6) patients who preventively use antibiotics are 2.675 times more likely to develop SAP than those who do not; (7) patients whose hospitalization periods are.> 20 days are 0.500 times more likely to develop SAP than those who do not; (8) patients who suffer from tracheal intubation are 2.980 times more likely to develop SAP than those who do not; and (9) patients who suffer from tracheal incision are 2.190 times more likely to develop SAP than those who do not. Conclusions: SAP was more closely related with diabetes, age, consciousness, days of hospitalization, tracheal intubation, tracheal incision, nasal feeding treatment, and the application of H2-receptor blocking agents and antimicrobials. © W. S. Maney & Son Ltd 2011.
Zhang C.,The First Affiliated Hospital |
Chen Y.,General Hospital of Beijing Military Region |
Fu X.,The First Affiliated Hospital
Cytotherapy | Year: 2015
Stem cells are the seeds of tissue repair and regeneration and a promising source for novel therapies. The skin of patients with an extensive deep burn injury is repaired by a hypertrophic scar without regeneration of sweat glands and therefore loses the function of perspiration. Stem cell therapy provides the possibility of sweat gland regeneration. In particular, recent studies have reported the reprogramming of mesenchymal stromal cells into sweat gland-like (SGL) cells. We present an overview of recent researches into sweat gland regeneration with stem cells. Difficulties of sweat gland regeneration after deep burns have been elaborated. The advantage and disadvantage of several stem cell types in sweat gland regeneration have been discussed. Additionally, the possible mechanisms for reprogramming stem cells to SGL cells are summarized. A brief discussion on clinical application of stem cell-derived SGL cells is also presented. This review may possibly provide some implications for sweat gland regeneration. © 2015 International Society for Cellular Therapy.
Qiu H.,The First Affiliated Hospital
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2012
To observe the effects on patients with HBeAg positive chronic hepatitis B by Baihua Xianglian Detoxification Recipe (BXDR) combined adefovir dipivoxil (AD), and to assess its clinical efficacy. A multi-center randomized clinical trial was performed, and 240 patients with HBeAg positive chronic hepatitis B (CHB) were randomly assigned to the experimental group and the control group. Patients in the experimental group were given AD 10 mg, once daily, while BXDR was additionally given those in the control group, twice daily. The treatment course was 48 weeks. The virologic, serologic, biochemical, chronic liver disease questionnaire (CLDQ) score, and adverse event were observed for 12, 24, and 48 weeks. (1) In aspect of virology: From the 12th week, statistical difference existed in the HBVDNA logarithm value between the experimental group and the control group (P < 0.05). The virologic response rate was 62.71% and 77.97% in the experimental group at the 12th and 24th week, while it was 49. 57% and 67. 52% in the control group, showing statistical difference (P < 0.05). There was no significant difference in the virological response rate at the 48th week (P > 0.05). The HBVDNA negative rate in the experimental group was 22.03% at the 12th week, 41.52% at the 24th week, and 55.08% at the 48th week. It was 11.11%, 21.37%, and 30.77% in the control group, showing statistical difference (P < 0.05). (2) In aspect of HBeAg/anti-HBe serology: The serum HBeAg response rate was 26.27% at the 24th week and 39.83% at the 48th week in the experimental group, while it was 13.68% at the 24th week and 29.06% at the 48th week in the control group, showing statistical difference (P < 0.05). The HBeAg negative conversion rate at the 48th week of treatment was 22.03% in the experimental group and 11.96% in the control group, showing statistical difference (P < 0.05). (3) In aspect of biochemistry: The biochemical response rate at the 24th week and the 48th week was 74.58% and 87.29% respectively in the experimental group, while it was 60.68% and 79.49% in the control group, showing statistical difference (P < 0.05). (4) In aspect of CLDQ score: After treatment the CLDQ scores in the two groups were higher compared with before treatment with statistical difference (P < 0.05). The CLDQ scores at the 24th week and the 48th week in the experimental group were superior to those in the control group, showing statistical difference (P < 0.05). (5) In aspect of adverse reactions: The main adverse reactions were headache, abdominal pain, nausea. During the study period, the total creatine kinase (CK) increased in 9 cases with the occurrence rate of 3.83%. In treating patients with HBeAg positive CHB, BXDR combined AD could significantly improve the inhibition of HBVDNA, increase the HBeAg seroconversion rates, accelerate the recovery of the liver function, improve the quality of life with higher safety.
Liping Z.,The First Affiliated Hospital
Pakistan journal of pharmaceutical sciences | Year: 2015
This paper was to explore the effect of health education frequency and patterns on the children. Children aged 3-12 years were randomly divided into Group A, B and C, respectively received the health education once a month, once ten days as well as once a week within a period of one year .It was as the evaluation both of the hospitalization frequency owing to the respiratory tract infections and the health education loss rate with year turnover. Results shown Group C was close to the times of hospitalization less than 3 times in one year, while Group B was to 3-6 as well as Group A was to the times of hospitalization 6-9 times in one year. The results of health education loss rate, which shown the loss increases with the frequency of the health education, was 28% in Group A, 36.36% in Group B and 46.34% in Group C. Conclusion could be draw as following: the health education frequency and patterns could affect the children by hospitalization rate of respiratory tract infection as well as the health education loss rate. It was a reasonable solution to give them the health education once every ten days.