The Fifth Peoples Hospital of Suzhou

Suzhou, China

The Fifth Peoples Hospital of Suzhou

Suzhou, China

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Zhou A.-P.,Shanghai JiaoTong University | Zhou A.-P.,Tibet University | Xu Z.-H.,Shanghai JiaoTong University | Sun Q.,Shanghai JiaoTong University | And 2 more authors.
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2012

Objective: To analyse the 5 MIRU loci of Mycobacterium tuberculosis, and investigate the relationship between polymorphisms of MIRU loci and expression of downstream genes. Methods: Bioinformatics method was used to predict the downstream genes promoter regions of 5 MIRU loci (ETR-C, Mtub-30, Mtub-39, MIRU-27 and MIRU- 40). Promoter sequence was amplified by PCR, and was cloned into mycobacterial promoterless probe vector pMC210 to generate the recombinants. After confirmation by restriction endonuclease digestion and sequence analysis, the recombinant plasmids were transformed into mycobacterium smegmatis mc2155 by electroporation. The transcriptional level of reporter gene lacZ was evaluated by Real-Time PCR, and the influence of polymorphisms of MIRU loci on the expression of downstream genes was examined. Results: Mtub-39 locus core region contained the promoter of the downstream gene. The recombinant plasmids harboring Mtub-39 locus with 1, 3 or 5 copy numbers were constructed. Real-Time PCR revealed that Mtub-39 locus with polymorphisms (pMC210-Mtub-39-N158, pMC210-Mtub-39-N139 and pMC210-Mtub-39-N146) had significant differences in the transcriptions of reporter gene lacZ (P=0.0065). Conclusion: The polymorphisms of Mtub-39 can significantly affect the promotor activity of the downstream genes, and sequentially regulate the expression of the gene.


Rui K.,Jiangsu University | Tian J.,Jiangsu University | Tang X.,Jiangsu University | Ma J.,Jiangsu University | And 6 more authors.
Immunologic Research | Year: 2016

Tumor-elicited immunosuppression is one of the essential mechanisms for tumor evasion of immune surveillance. It is widely thought to be one of the main reasons for the failure of tumor immunotherapy. Myeloid-derived suppressor cells (MDSCs) comprise a heterogeneous population of cells that play an important role in tumor-induced immunosuppression. These cells expand in tumor-bearing individuals and suppress T cell responses via various mechanisms. Curdlan, the linear (1 → 3)-β-glucan from Agrobacterium, has been applied in the food industry and other sectors. The anti-tumor property of curdlan has been recognized for a long time although the underlying mechanism still needs to be explored. In this study, we investigated the effect of curdlan on MDSCs and found that curdlan could promote MDSCs to differentiate into a more mature state and then significantly reduce the suppressive function of MDSCs, decrease the MDSCs in vivo and down-regulate the suppression in tumor-bearing mice, thus leading to enhanced anti-tumor immune responses. We, therefore, increase the understanding of further mechanisms by which curdlan achieves anti-tumor effects. © 2016, Springer Science+Business Media New York.


PubMed | Jiangsu University, The Fifth Peoples Hospital of Suzhou and University of Hong Kong
Type: Journal Article | Journal: Immunologic research | Year: 2016

Tumor-elicited immunosuppression is one of the essential mechanisms for tumor evasion of immune surveillance. It is widely thought to be one of the main reasons for the failure of tumor immunotherapy. Myeloid-derived suppressor cells (MDSCs) comprise a heterogeneous population of cells that play an important role in tumor-induced immunosuppression. These cells expand in tumor-bearing individuals and suppress T cell responses via various mechanisms. Curdlan, the linear (13)--glucan from Agrobacterium, has been applied in the food industry and other sectors. Theanti-tumorpropertyofcurdlan has been recognized for a long time although the underlying mechanism still needs to be explored. In this study, we investigated the effect of curdlan on MDSCs and found that curdlan could promote MDSCs to differentiate into a more mature state and then significantly reduce the suppressive function of MDSCs, decrease the MDSCs in vivo and down-regulate the suppression in tumor-bearing mice, thus leading to enhanced anti-tumor immune responses. We, therefore, increase the understanding of further mechanisms by which curdlan achieves anti-tumor effects.


Wang Z.R.,Soochow University of China | Wang Z.R.,The Fifth Peoples Hospital of Suzhou | Wang J.H.,Soochow University of China | Hu C.L.,Soochow University of China | And 5 more authors.
Brazilian Journal of Medical and Biological Research | Year: 2011

Searching for effective Smad3 gene-based gene therapies for hepatic fibrosis, we constructed siRNA expression plasmids targeting the rat Smad3 gene and then delivered these plasmids into hepatic stellate cells (HSCs). The effect of siRNAs on the mRNA levels of Smad2, Smad3, Smad4, and collagens I-α1, III-α1 and IV-α1 (Col1α1, Col3α1, Col4α1, respectively) was determined by RT-PCR. Eighty adult male Sprague-Dawley rats were randomly divided into three groups. Twice a week for 8 weeks, the untreated hepatic fibrosis model (N = 30) and the treated group (N = 20) were injected subcutaneously with 40% (v/v) carbon tetrachloride (CCl 4)-olive oil (3 mL/kg), and the normal control group (N = 30) was injected with olive oil (3 mL/ kg). In the 4th week, the treated rats were injected subcutaneously with liposome-encapsulated plasmids (150 μg/kg) into the right liver lobe under general anesthesia once every 2 weeks, and the untreated rats were injected with the same volume of buffer. At the end of the 6th and 8th weeks, liver tissue and sera were collected. Pathological changes were assessed by a semi-quantitative scoring system (SSS), and a radioimmunoassay was used to establish a serum liver fibrosis index (type III procollagen, type IV collagen, laminin, and hyaluronic acid). The mRNA expression levels of the above cited genes were reduced in the HSCs transfected with the siRNA expression plasmids. Moreover, in the treated group, fibrosis evaluated by the SSS was significantly reduced (P < 0.05) and the serum indices were greatly improved (P < 0.01). These results suggest that Smad3 siRNA expression plasmids have an anti-fibrotic effect.


PubMed | Nanjing Medical University, The Fifth Peoples Hospital of Suzhou and Soochow University of China
Type: Journal Article | Journal: Biochemical and biophysical research communications | Year: 2015

The Leucine rich repeat containing G protein coupled receptor 5 (LGR5), may be a candidate marker of non-small cell lung cancer (NSCLC) cells with stem cell-like properties. Aldehyde dehydrogenase 1A1 (ALDH1A1) is one of NSCLC stem cell markers. To identify the relationship of LGR5 and ALDH1A1 in NSCLC, we analyzed the expression of LGR5 and ALDH1A1 in NSCLC samples, and determined their clinical significance. We performed quantitative RT-PCR for LGR5 and ALDH1A1 expression in 24 NSCLC patients, and showed that LGR5 and ALDH1A1 mRNA were frequently increased in NSCLC tissues in comparison to that in adjacent normal tissues (p = 0.0005 and p < 0.0001, respectively). Besides, the expression of LGR5 and ALDH1A1 mRNA has a significant correlation (r = 0.416, P = 0.0483). The expression of LGR5 and ALDH1A1 in 109 NSCLC tumors and 50 adjacent normal tissues were detected by immunohistochemistry. Positive LGR5 and ALDH1A1 expression was defined in 28.4% and 41.3% of the NSCLC tumors, respectively. Further analysis indicated that 24 of these LGR5 (24/31) samples expressed ALDH1A1(r = 0.3883, p < 0.0001), we also found co-localization of LGR5 and ALDH1A1 in tumor tissue samples. LGR5 and ALDH1A1 expression was significantly associated with higher pathological TNM stage of the disease (stage I + II and III + IV) (P = 0.0311 and p = 0.0221, respectively), the co-expression of LGR5 and ALDH1A1 was associated with nodal status (p = 0.0424). High expression of LGR5 or ALDH1A1 was related to poor prognosis (P = 0.0125 and p = 0.0410, respectively), and NSCLC patients with co-expression of LGR5 and ALDH1A1 had a poorer prognosis than the others (P = 0.0011). Both of them can be an independent risk factor of a poorer prognosis (P = 0.016 and P = 0.024, respectively). The expression of LGR5 and ALDH1A1 were closely associated with the tumorigenicity, metastasis and poor prognosis of NSCLC, and LGR5 cells in NSCLC were likely to be the cancer cells with stem cell-like properties due to the significant correlation between LGR5 and ALDH1A1.


Zhao J.,The Fifth Peoples Hospital of Suzhou | Wang F.-P.,The Fifth Peoples Hospital of Suzhou | Sun Q.-Q.,The Fifth Peoples Hospital of Suzhou | Yang Y.-T.,The Fifth Peoples Hospital of Suzhou | Chen L.,The Fifth Peoples Hospital of Suzhou
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2015

Objective: To explore the application value of DNA micro-array method for quick detection of drug resistant genes of Mycobacterium tuberculosis (M. tuberculosis). Methods: DNA micro-array method was adopted to detect the M. tuberculosis in sputum samples of clinically suspicious TB patients. The wild genotypes and different mutant genotypes of promoters of three genes relevant to resistance of rifampicin (RFP) and isoniazid (INH), i.e. rpoB, katG, and inhA, were detected. BACTECTM MGIT 960 system was used to cultivate M. tuberculosis and the modified Roche proportion method was adopted to perform drug susceptibility for positive liquid cultures. Results: Among 174 positive liquid cultures, 15 of them were rpoB mutant genotype. The mutation frequency of locus 531 was the highest and mutation rate was 66. 7%. Seventeen cultures were katG/inhA mutant genotype and 12 of them were single mutation of katG with the mutation rate of 70.6%. Compared with the results of 68 cultures detected by the modified Roche proportion method, the detection rate of DNA micro-array method for detecting RFP and INH resistance was more than 90%. Conclusion: DNA micro-array method can quickly and reliably detect the gene mutation of rpoB, katG, and inhA in most clinical samples of suspicious TB patients. This method can be used to detect clinical drug resistance and guide clinical medication and diagnosis. © 2015, Editorial Department of Journal of Shanghai Second Medical University. All right reserved.


Qian F.,The Fifth Peoples Hospital Of Suzhou | Li M.,The Fifth Peoples Hospital Of Suzhou | Zhu C.-W.,The Fifth Peoples Hospital Of Suzhou
World Chinese Journal of Digestology | Year: 2015

Chronic hepatitis caused by hepatitis B virus (HBV) infection remains an incurable disease at present, which is mainly because the approved antiviral agents, such as interferon-alpha and nucleos(t)ide analogues, cannot effectively eradicate intrahepatic hepatitis B virus covalently closed circular DNA (cccDNA). And thus a suboptimal efficacy of antiviral agents and relapse after therapy occur very commonly. Therefore, novel drugs and treatment strategies remain to be developed on the basis of further theoretical and clinical research of HBV infection to achieve the ultimate goal of eradication of HBV cccDNA in the future. In this paper, we discuss multiple agents and therapeutic regimens influencing cccDNA levels, in order to help clinicians comprehensively understand the present situation in the research of the clearance of HBV cccDNA. © 2015 Baishideng Publishing Group Inc. All rights Reserved.


Ping X.,The Fifth Peoples Hospital of Suzhou | Junchi X.,The Fifth Peoples Hospital of Suzhou | Xinnian C.,The Fifth Peoples Hospital of Suzhou | Zhijian Y.,The Fifth Peoples Hospital of Suzhou | Meiying W.,The Fifth Peoples Hospital of Suzhou
Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG / World Association of Sarcoidosis and Other Granulomatous Disorders | Year: 2015

A 23-year-old woman had dry cough, fever and chest tightness for 1 months. Through thoracic CT scan and serological examination, the patient was clinically diagnosed as disseminated tuberculosis. she was given anti-tuberculosis therapy combined with autologous cytokine-induced killer (CIK) immunotherapy. Through the close follow-ups we found that after immunotherapy Her condition would have a swift improvement and she do not appear liver damage after a large doses of antibiotic therapy. In conclusion, adjuvant autologous CIK immunotherapy is an effective approach for disseminated tuberculosis.


PubMed | The Fifth Peoples Hospital of Suzhou
Type: Case Reports | Journal: Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG | Year: 2015

A 23-year-old woman had dry cough, fever and chest tightness for 1 months. Through thoracic CT scan and serological examination, the patient was clinically diagnosed as disseminated tuberculosis. she was given anti-tuberculosis therapy combined with autologous cytokine-induced killer (CIK) immunotherapy. Through the close follow-ups we found that after immunotherapy Her condition would have a swift improvement and she do not appear liver damage after a large doses of antibiotic therapy. In conclusion, adjuvant autologous CIK immunotherapy is an effective approach for disseminated tuberculosis.


PubMed | The Fifth Peoples Hospital of Suzhou
Type: Journal Article | Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy | Year: 2013

Trem-like transcript 2 (TLT-2), one of the TREM family members, which is expressed on B cells, T cells, and macrophages, plays a critical role in immune response mechanism. In this study, two novel mouse anti-human TLT-2 monoclonal antibodies (MAbs) were prepared using hybridoma technology and their immunological characteristics were determined. The results showed that the two MAbs (clones 10F5 and 8C10) were both IgG1 () and bound specifically to human TLT-2. Furthermore, 10F5 and 8C10 seemed to recognize a different site (epitope) of TLT-2 by competition assay. MAb 10F5 was proven in Western blot analysis to specifically bind to denatured TLT-2 protein while both MAbs were proven in dot blot analyses and immunofluorescence to specifically bind to natural TLT-2 protein. In addition, crosslinking of TLT-2 with MAb 8C10 markedly blocked TLT-2 positive signal and T cell proliferation. Taken together, these two monoclonal antibodies might be of great value as tools for further exploration of the expression and function of TLT-2.

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