The Fifth Peoples Hospital Of Jinan City

Jinan, China

The Fifth Peoples Hospital Of Jinan City

Jinan, China

Time filter

Source Type

Qu A.,Zuoping Hospital Affiliated to Taishan Medical College | Han X.,Shandong University of Traditional Chinese Medicine | Wang Y.G.,Shandong University | Liu C.H.,Shandong University | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

Objective: This study is to investigate the effects of the lymphocyte co-culture on the proliferation and apoptosis of the hepatitis B virus (HBV)-expressing normal human mesangial cells (NHMCs). Methods: NHMCs were transfected with the PHY106-CHBV plasmid containing C genotypeHBV with liposome. These cells were co-cultured with human peripheral blood lymphocytes. The HBsAg and HBeAg contents in the supernatant were detected at 24 h, 48 h, and 72 h after transfection. Cell proliferation was assessed with the MTT assay, and apoptosis was detected with flow cytometry. Results: In the normal control and negative control groups, negative results for the HBsAg and HBeAg detection were observed, at all the time points. However, in the transfected NHMCs, positive results were observed for the HBsAg and HBeAg detection at 48 h and 72 h after transfection. These HBV-expressing NHMCs were co-cultured with human peripheral blood lymphocytes for 48 h. Results from the MTT assay showed that, the proliferation of NHMCs was not significantly affected by the transfection of the empty plasmid. However, compared with the control group, the proliferation rate was dramatically declined in HBV-expressing NHMCs. Moreover, the lymphocyte co-culture further significantly decreased the proliferation of HBV-expressing NHMCs. In addition, our results from the flow cytometry showed that, the early apoptosis rate in the co-culture group was significantly higher than the HBV-expressing group. Conclusion: Co-culture of peripheral blood lymphocytes could inhibit proliferation and enhance apoptosis of HBV-expressing NHMCs. These findings might contribute to the understanding of the pathogenic mechanisms of the HBV-associated glomerulonephritis. © 2016, E-Century Publishing Corporation. All rights reserved.


Yin X.-C.,Jinan Stomatological Hospital | Xie P.-F.,Jinan Stomatological Hospital | Liu L.,The Fifth Peoples Hospital Of Jinan City
Journal of Biomaterials and Tissue Engineering | Year: 2016

In the present study, a new tumor-penetrating peptide, CRGDK-surface modified nanomicelle was formulated to enhance the chemotherapeutic efficiency of sunitinib in squamous cell carcinoma. We have showed that CRGDK substitution on the surface of nanoparticles enhanced the tumor targeting prospect of drug-loaded nanomicelles and could be a promising candidate for targeting squamous cell carcinoma. The nanoparticles were formulated in nanosized dimensions and showed a controlled drug release profile. The presence of CRGDK peptide remarkably enhanced the nanoparticle uptake in SCC-15 cancer cells. The targeting efficiency of PSNP was proved by both fluorescence and flow cytometer analysis. Furthermore, CLSM analysis showed a higher fluorescence in the cell cytoplasm indicating the higher cellular internalization via endocytosis mechanism. CRGDK-surface modified nanomicelles significantly killed the cancer cells than comparing to either non-targeted SNP or free drug indicating the excellent targeting efficiency of nanomicelles towards the OSCC cells. PSNP induced significant apoptosis-related morphological alterations, such as apoptotic body formation and chromatin condensation in squamous cell carcinoma. Annexin-V/PI staining assay showed a remarkable early and late apoptosis cells after the treatment of PSNP formulations. The superior cytotoxic effect of PSNP could be attributed due to the higher internalization of PSNP inside the cells (due to the surface modification of CRGDK peptide) and continuous exposure and sustained release of the drug for a prolonged period of time at the site of action. Overall, CRGDK-conjugated nanomicelles could be an interesting prospect for the treatment of squamous cell carcinoma. © 2016 American Scientific Publishers.


Liu L.,The Fifth Peoples Hospital Of Jinan City | Wei Z.,Jinan Stomatological Hospital | Sun Z.,The Fifth Peoples Hospital Of Jinan City | Yin X.,Jinan Stomatological Hospital
International Journal of Clinical and Experimental Medicine | Year: 2016

Previous studies indicated that human papilloma virus (HPV) infection may be associated with risk of oral squamous cell carcinomas (OSCC). In this study, we evaluate the relationship OSCC and HPV infection in Chinese population via meta-analysis. A total of 7 articles were included in our study eligible case-control studies. After testing the heterogeneity of the studies by the Cochran Q test, the meta-analyses for HPV and HPV16 were performed using the random effects model. Quantitative meta-analyses showed that subjects OSCC with HPV infection increase 6.03 times risk of OSCC than subjects without HPV infection (OR=7.03, 95% CI: 3.88-12.76). High incidences of HPV infection may increase risk of OSCC In Chinese population. © 2016, E-Century Publishing Corporation. All rights reserved.


Jia Z.,The Fifth Peoples Hospital Of Jinan City | Liu Y.,The Fifth Peoples Hospital Of Jinan City | Su H.,The Fifth Peoples Hospital Of Jinan City | Li M.,The Fifth Peoples Hospital Of Jinan City | And 5 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

The Heart failure (HF) is considered as the end-stage of various heart disease and associated with high mortality globally. Progressive loss of cardiac myocytes via apoptosis is considered as the most important factor for HF pathology. In this study, we demonstrated that Safflower extract was able to inhibitthe apoptosis inducted by Angiotensin II (AngII) in a ratmyocardium derived cell line H9C2. Further examination of LC-3II conversion and autophagosome formation suggested Safflower extract induced autophagy in treated cell. Inhibition of Safflower extract induced autophagy by 3-methyladenine (3MA) abolished anti-apoptotic function of Safflower extract, while application of autophagy stimulator Rapamycin in H9C2 inhibited apoptosis as well. Moreover, treatment of H9C2 cell with Safflower extract also inhibited expression of pro-apoptotic genes BAD and Bax. In conclusion, our data indicated that Safflower extract inhibit apoptosis via inducing autophagy in myocardium cell and demonstrated the potential as novel therapeutic drug for Heart failure. © 2015 E-Century Publishing Corporation. All rights reserved.

Loading The Fifth Peoples Hospital Of Jinan City collaborators
Loading The Fifth Peoples Hospital Of Jinan City collaborators