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Yinchuan, China

Hao G.,Affiliated Hospital of the Academy of Military Medical science | Bai S.,The Fifth Hospital of PLA | Liang H.,Capital Medical University | Liang Y.,Affiliated Hospital of the Academy of Military Medical science | And 6 more authors.
Experimental and Therapeutic Medicine | Year: 2013

A rapid, sensitive and specific HPLC-MS/MS method was developed and validated for the quantification of potassium oxonate (Oxo) in human plasma using [13C2,15N3]-Oxo as an internal standard (IS). The target substance was separated from human plasma using the solid-phase extraction method. Chromatography separation was performed on a Waters:Atlantis dC18 column (150x4.6 mm, 5.0 μm) with a mobile phase consisting of H2O with 0.1% formic acid in acetonitrile (90:10, v/v). The mass spectrometer works with electrospray ionization and multiple reaction monitoring in its negative ion mode, using target ions at [M-H]- m/z 111.9 for Oxo and [M-H]- m/z 117.0 for the IS. The mean standard curve was linear (r=0.9991) over the concentration range of 2.0-200.0 ng/ml and had good back-calculated accuracy and precision. The intra- and inter-day precision were <6.33% and the accuracy was >99.38%. The extraction recovery was >60.26%. The lower limit of quantification achieved with this method was 2.0 ng/ml. This assay method was demonstrated to be accurate, sensitive and simple and was successfully applied to a pharmacokinetic study following single oral administration of a 40-mg S-1 capsule in 12 tumor patients. Source


Song R.,The Fifth Hospital of PLA | Zhu S.,Chongqing Medical University | Zhang H.,The General Hospital of Jinan Military Region | Li X.,The General Hospital of Jinan Military Region | Wu S.,Renmin University of China
International Journal of Clinical and Experimental Medicine | Year: 2014

Objective: This study aims to explore the expression and significance of ATPase in myocardium of congestive heart failure induced by pressure overload in rats. Methods: Male SD rats were divided into 3 groups randomly: Control group (C group), coarctation of abdominal aorta 15 weeks group (A group) and sham operation group (SH group). The hemodynamics parameters were measured. The size of adenine acid pool (ATP, ADP) in myocardium was measured by high performance liquid chromatography (HPLC). The mRNA copies of ATPase-α subunit, ATPase-6 subunit in ventricular myocardium were determined by RT-PCR. The protein content of F1-ATPase in mitochondria was detected by Western blot analysis. Results: The ±dp/dtmax and contents of ATP and ADP decreased significantly in myocardium were decreased in A group. Compared with C and SH group, the mRNA copies of ATPase-α subunit, ATPase -6 subunit and the protein content of F1-ATPase were decreased in A group. Conclusion: The expression of F1-ATPase decreased parallel with abnormal of the adenine nucleotide in myocardium of congestive heart failure. It may play a critical role in disturbance of energy metabolism in disfunction of heart. © 2014 E-Century Publishing Corporation. All rights reserved. Source


Liang H.,The Fifth Hospital of PLA | Gong W.,The Fifth Hospital of PLA | Ji H.-B.,The Fifth Hospital of PLA | Kang H.-L.,The Fifth Hospital of PLA
Journal of Practical Oncology | Year: 2013

To investigate the effect of HIF-1α on the expression of GLUT-1 in human hepatocellular carcinoma HepG2 cells cultured under hypoxia. Hepatoma carcinoma cell line HepG2, cultivated in DMEM medium with fetal bovine serum, was divided into control group (normal oxygen, 21% O2), hypoxia group (1% O2), normal oxygen with indomethacin group and hypoxia with indomethacin group. After the cells were harvested, the expression and nucleus translocation of HIF-1α in cells of each group were investigated through immunofluorescence method. GLUT-1 protein and HIF-1α protein in each group were tested by Western blot, respectively. The amount of GLUT-1 mRNA in each group was tested by RT-PCR. The expressions of HIF-1α (protein: 68.25 ± 11.72) and GLUT-1 (mRNA: 0.7424 ± 0.1127; protein: 70.33 ± 12.83) increased in hepatocellular carcinoma HepG2 cells cultured under hypoxia (P< 0.05), and meanwhile the expressions of GLUT-1 mRNA (0.3710 ± 0.1016) and protein (38.46± 12.20) were reduced since the stabilization and accumulation of HIF-1α protein (36.34 ± 9.50) were inhibited by indomethacin in anaerobic condition (P < 0.05). Hypoxia condition can upregulate the expressions of GLUT-1 and HIF-1α in hepatoma carcinoma HepG2 cells. HIF-1α can regulate the transcription and expression of GLUT-1, thereby influence the glycolysis and cellular energy production. Source


Liang H.,The Fifth Hospital of PLA | Ji H.-B.,The Fifth Hospital of PLA | Gong W.,The Fifth Hospital of PLA | Ma L.-Q.,The Fifth Hospital of PLA | And 2 more authors.
Journal of Practical Oncology | Year: 2014

Objective: To investigate the relationship between the expression of hypoxia inducible factor-1α(HIF-1α) and glucose transporter 1(Glut1) in hepatocellular carcinoma(HCC) and its clinical significance. Methods: RT-PCR was used to detect the expression of HIF-1α mRNA and Glut1 mRNA in samples from 28 HCC cases and corresponding tumor-adjacent normal tissues. Immunohistochemical staining Envision method was used to detect the expression of HIF-1α and Glut1 in HCC tissues from 47 cases and tumor-adjacent normal tissues from 10 cases. Results: The results of RT-PCR were consistent with those of immunohistochemical analysis. The positive expression rates of HIF-1α and Glut1 in HCC tissues were higher than those in tumor-adjacent normal tissues(P<0.05). There was association between the expression of HIF-1α and Glut1 and tumor size, pathology grade, clinical stage, lymphatic metastasis (P<0.05). With the increase of HIF-1α expression, the expression of Glut1 increased; positive correlation was found between HIF-1α and Glut1 expression(r=0.6228, P=0.0004). Conclusions: The overexpression of HIF-1α and Glut1 in HCC tissues is closely related to the malignant development, invasion and metastasis of HCC. Detection of both HIF-1α and Glut1 can facilitate the determination of HCC clinical stage and the evaluation of its prognosis. There may be a mechanism that the expression of Glut1 is up-regulated by HIF-1α, which facilitates the origin of HCC. Source

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