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Tu Z.-Q.,The Eighth Peoples Hospital of Shanghai | Li R.-J.,the Peoples Hospital of Zhengzhou | Mei J.-Z.,the Peoples Hospital of Zhengzhou | Li X.-H.,The Eighth Peoples Hospital of Shanghai
International Journal of Clinical and Experimental Pathology | Year: 2014

Introduction: Long non-coding RNAs (lncRNAs) have been investigated as a new class of regulators of cellular processes, such as cell growth, apoptosis, and carcinogenesis. lncRNA GAS5 has recently been identified to be involved in tumorigenesis of several cancers such as breast cancer, lung cancer and renal cancer. However, the regulation of GAS5 in hepatocellular carcinoma (HCC) has not yet been reported before. Methods: Expression of GAS5 in tumor and their normal matched tissues was determined by quantitative real-time PCR in n = 71 HCC patients, and its association with overall survival of patients was analyzed by statistical analysis. Results: The expression level of GAS5 was reduced in HCC in comparison to normal matched tissues (P < 0.05). It is also proved that GAS5 expression was to be associated with HCC tumor size, lymphnode metastasis and clinical stage (P < 0.05). In addition, the Kaplan-Meier survival curves revealed that low GAS5 expression was associated with poor prognosis in HCC patients. GAS5 expression was an independent prognostic marker of overall HCC patient survival in a multivariate analysis. Conclusions: The study proved for the first time that GAS5 down regulated in a majority of HCC patients. Our results indicated that GAS5 expression was an independent prognostic factor for patients with liver cancer, which might be a potential valuable biomarker for HCC.

Yu P.-j.,Jiangsu University | Yu P.-j.,Shanghai JiaoTong University | Wang L.-p.,The Eighth Peoples Hospital of Shanghai | Guo Y.,The Eighth Peoples Hospital of Shanghai | And 2 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011

BACKGROUND: Studies in vitro have confirmed that collagen-chitosan (80:20) compound nanofiber membranes have excellent mechanical properties, biocompatibility and biodegradability. However, there are few researches in vivo about the materials. OBJECTIVE: To observe the effect of collagen-chitosan compound nanofiber membranes on repair of full-thickness skin defect on the back of Sprague Dawley (SD) rats. METHODS: Thirty SD rats with full-thickness skin defects on the back were divided into two groups randomly by lottery method. SD rats treated with collogen-chitosan (80:20) compound nanofiber membrane and oily gauzes on the defects were taken as the experimental group, while those treated with oily gauzes only as the control group. The defects of all rats were fixed with dry gauze bandage at the margin of outer packaging. RESULTS AND CONCLUSION: The wounds healed basically in the experimental group at 14 days after the repair; hematoxylin-eosin staining showed that the numbers of capillaries were reduced and the content of fibers was increased. While in the control group, wound healed irregularly, and the wound was larger than that in the experimental group; hematoxylin-eosin staining showed that there were more obvious dilated capillaries and a large number of inflammatory cells. The above results indicated that collagen-chitosan compound nanofiber membrane is superior to ordinary gauze in promoting the tissue repair and wound healing of skin defects.

Huan J.,The Eighth Peoples Hospital of Shanghai | Wang L.,Shanghai JiaoTong University | Xing L.,The Eighth Peoples Hospital of Shanghai | Qin X.,The Eighth Peoples Hospital of Shanghai | And 3 more authors.
Gene | Year: 2014

Objective: Estrogens are known to regulate the proliferation of breast cancer cells and to alter their cytoarchitectural and phenotypic properties, but the gene networks and pathways by which estrogenic hormones regulate these events are only partially understood. Methods: We used global gene expression profiling by Affymetrix GeneChip microarray analysis, with KEGG pathway enrichment, PPI network construction, module analysis and text mining methods to identify patterns and time courses of genes that are either stimulated or inhibited by estradiol (E2) in estrogen receptor (ER)-positive MCF-7 human breast cancer cells. Results: Of the genes queried on the Affymetrix Human Genome U133 plus 2.0 microarray, we identified 628 (12. h), 852 (24. h) and 880 (48. h) differentially expressed genes (DEGs) that showed a robust pattern of regulation by E2. From pathway enrichment analysis, we found out the changes of metabolic pathways of E2 treated samples at each time point. At 12. h time point, the changes of metabolic pathways were mainly focused on pathways in cancer, focal adhesion, and chemokine signaling pathway. At 24. h time point, the changes were mainly enriched in neuroactive ligand-receptor interaction, cytokine-cytokine receptor interaction and calcium signaling pathway. At 48. h time point, the significant pathways were pathways in cancer, regulation of actin cytoskeleton, cell adhesion molecules (CAMs), axon guidance and ErbB signaling pathway. Of interest, our PPI network analysis and module analysis found that E2 treatment induced enhancement of PRSS23 at the three time points and PRSS23 was in the central position of each module. Text mining results showed that the important genes of DEGs have relationship with signal pathways, such as ERbB pathway (AREG), Wnt pathway (NDP), MAPK pathway (NTRK3, TH), IP3 pathway (TRA@) and some transcript factors (TCF4, MAF). Conclusions: Our studies highlight the diverse gene networks and metabolic and cell regulatory pathways through which E2 operates to achieve its widespread effects on breast cancer cells. © 2013 Elsevier B.V.

Huan J.L.,The Eighth Peoples Hospital of Shanghai | Gao X.,Yidu Center Hospital of Weifang City | Xing L.,The Eighth Peoples Hospital of Shanghai | Qin X.J.,The Eighth Peoples Hospital of Shanghai | And 3 more authors.
Genetics and Molecular Research | Year: 2014

The aim of this study was to identify key genes related to invasive ductal carcinoma (IDC) of the breast by analyzing gene expression data with bioinformatic tools. Microarray data set GSE31138 was downloaded from Gene Expression Omnibus, including 3 breast cancer tissue samples and 3 normal controls. Differentially expressed genes (DEGs) between breast cancer and normal control were screened out (FDR< 0.05 and |logFC| > 2). Coexpression between genes was examined with String, and a network was then constructed. Relevant pathways and diseases were retrieved with KOBAS. A total of 56 DEGs were obtained in the IDC of the breast compared with normal controls. A gene coexpression network including 27 pairs of genes was constructed and all the genes in the network were upregulated. Further study indicated that most of the genes in the coexpression network were enriched in ECM-receptor interaction (COL4A2, FN1, and HMMR) and nucleotide excision repair (CETN2 and PCNA) pathways, and that the most significantly related disease was autoimmune lymphoproliferative syndromes. A number of DEGs were acquired through comparative analysis of gene expression data. These findings are beneficial in promoting the understanding of the molecular mechanisms in breast cancer. More importantly, some key genes were revealed via gene coexpression network analysis, which could be potential biomarkers for IDC of the breast. © FUNPEC-RP.

Huan J.-L.,The Eighth Peoples Hospital of Shanghai | Xing L.,The Eighth Peoples Hospital of Shanghai | Qin X.-J.,The Eighth Peoples Hospital of Shanghai | Gao Z.-G.,The Eighth Peoples Hospital of Shanghai | And 2 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2012

Osteopontin (OPN) is an integrin-binding protein, believed to be involved in a variety of physiological cellular functions. The physiology of OPN is best documented in the bone where this secreted adhesive glycoprotein appears to be involved in osteoblast differentiation and bone formation. In our study, we used semi-quantitative RT-PCR of osteopontin in calcification tissue of breast to detect breast cancer metastasis. The obtained data indicate that the expression of osteopontin is related to calcification tissue of breast, and possibly with the incidence of breast cancer. The expression strength of OPN by RT-PCR detection was related to the degree of malignancy of breast lesions, suggesting a close relationship between OPN and breast calcification tissue. The results revealed that expression of OPN mRNA is related to calcification of breast cancer tissue and to the development of breast cancer. Determination of OPN mRNA expression can be expected to be a guide to clinical therapy and prediction of the prognosis of breast cancer patients.

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