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Homa S.T.,The Doctors Laboratory | Vessey W.,The Doctors Laboratory | Perez-Miranda A.,The Doctors Laboratory | Riyait T.,The Doctors Laboratory | Agarwal A.,Cleveland Clinic
Journal of Assisted Reproduction and Genetics | Year: 2015

Purpose: High levels of reactive oxygen species (ROS) are a leading cause of male factor infertility. Measurement of ROS has been hampered by a lack of standardisation and confounding variables including choice of controls and sample selection. This study aimed to determine a reference range for ROS in human semen. Methods: Semen samples were obtained from men attending for routine semen analysis who gave informed consent for the study. Samples were assigned groups: Group 1 (N = 94) normal semen parameters, no leucocytospermia; Group 2 (N = 100) abnormal semen parameters, no leucocytospermia; Group 3 (N = 41) any semen parameters with leucocytospermia. ROS levels were assayed in fresh neat semen using a chemiluminescence assay measured in a single tube luminometer. Data are reported in relative light units (RLU)/sec/106 sperm Results: ROS levels were significantly different between Groups 1, 2 and 3 (19.75 ± 8.12, 95.03 ± 33.63, 890.17 ± 310.23 RLU/sec/106 sperm respectively; p < 0.001). Group 3 gave the highest value confirming this group as the optimum choice for positive controls. The reference range < 24.1 RLU/sec/106 sperm was determined by ROC analysis that differentiates a reference population (Group 1) from a positive control group (Group 3), optimising the sensitivity and specificity (80.5 and 87.2 % respectively) of the test. Conclusions: We have determined a reference range for ROS in human semen and identified a patient population that falls outside the normal range. This simple, cost effective assay can be incorporated into routine diagnostic testing to aid in the diagnosis of male infertility, especially with regard to unexplained infertility. © 2015, Springer Science+Business Media New York. Source


Smelov V.,Karolinska Institutet | Smelov V.,North State Medical University | Novikov A.,North State Medical University | Brown L.J.,The Doctors Laboratory | And 6 more authors.
International Journal of STD and AIDS | Year: 2013

Symptomatic male urethral Chlamydia trachomatis infection resulted in inflammation of the prostate, with associated increases in both prostate-specific (PSA) and prostate cancer-specific (PCA3) markers with prostate biopsies showing no evidence of malignancy. © The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav. Source


Smelov V.,Karolinska Institutet | Smelov V.,Saint Petersburg State University | Arroyo Muhr L.S.,Karolinska Institutet | Bzhalava D.,Karolinska Institutet | And 3 more authors.
Journal of Medical Virology | Year: 2014

To investigate which microorganisms may be present in expressed prostate secretions (EPS) metagenomic sequencing (MGS) was applied to prostate secretion samples from five men with prostatitis and five matched control men as well as to combined expressed prostate secretion and urine from six patients with prostate cancer and six matched control men. The prostate secretion samples contained a variety of bacterial sequences, mostly belonging to the Proteobacteria phylum. The combined prostate secretion and urine samples were dominated by abundant presence of the JC polyomavirus, representing >20% of all detected metagenomic sequence reads. There were also other viruses detected, for example, human papillomavirus type 81. All combined prostate secretion and urine samples were also positive for Proteobacteria. In summary, MGS of expressed prostate secretion is informative for detecting a variety of bacteria and viruses, suggesting that a more large-scale use of MGS of prostate secretions may be useful in medical and epidemiological studies of prostate infections. J. Med. Virol. 86:2042-2048, 2014. © 2014 Wiley Periodicals, Inc. Source

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