Song T.,Huazhong Agricultural University |
Song T.,The Cooperative Innovation Center for Sustainable Pig Production |
Yang Y.,Huazhong Agricultural University |
Yang Y.,The Cooperative Innovation Center for Sustainable Pig Production |
And 6 more authors.
Cellular and Molecular Life Sciences | Year: 2017
It is well known that adipose tissue has a critical role in the development of obesity and metabolic diseases and that adipose tissue acts as an endocrine organ to regulate lipid and glucose metabolism. Accumulating in the adipose tissue, fatty acids serve as a primary source of essential nutrients and act on intracellular and cell surface receptors to regulate biological events. G protein-coupled receptor 120 (GPR120) represents a promising target for the treatment of obesity-related metabolic disorders for its involvement in the regulation of adipogenesis, inflammation, glucose uptake, and insulin resistance. In this review, we summarize recent studies and advances regarding the systemic role of GPR120 in adipose tissue, including both white and brown adipocytes. We offer a new perspective by comparing the different roles in a variety of homeostatic processes from adipogenic development to adipocyte metabolism, and we also discuss the effects of natural and synthetic agonists that may be potential agents for the treatment of metabolic diseases. © 2017 Springer International Publishing
He Q.,Huazhong Agricultural University |
He Q.,The Cooperative Innovation Center for Sustainable Pig Production |
Xi J.,Biogas Institute of Ministry of Agriculture |
Wang W.,Huazhong Agricultural University |
And 5 more authors.
International Journal of Greenhouse Gas Control | Year: 2017
As a by-product of anaerobic biogas digestion using biomass as the substrates, raw biogas slurry (RBS) may be a renewable CO2 solvent to capture CO2 and fix CO2 into the crops/plants by forming organic carbon through carbon concentration mechanism (CCM). However its CO2 absorption performance should be enhanced greatly without deteriorating its inherent low phytotoxicity. Recovery and enhancement of CO2 absorption performance of RBS with initial total ammonia nitrogen (TAN) concentration (∼0.152 mol-N/L) was investigated through stripping CO2 saturated in RBS during anaerobic digestion by vacuum regeneration technology. Additionally, phytotoxicity of the regenerated RBS (RegBS) after CO2 reabsorption was also evaluated in terms of EC50 value which represents the CO2-rich RegBS application concentration causing 50% inhibition to root elongation of mungbean seeds. Results showed that vacuum regeneration technology is a time-effective method to regenerate CO2 from RBS. And the activation energy of CO2 regeneration from RBS under 20 kPa and 35–75 °C is about 57.78 kJ/mol. The main mechanism of CO2 regeneration from RBS may be the decomposition of ammonium bicarbonate. CO2 reabsorption capacity of RegBS is enhanced up to 0.125 mol/L at 77 °C and 40 kPa, which is approximately 3.18-fold higher than that of RBS. If RBS with high TAN concentration can be adopted, much higher CO2 reabsorption capacity may be achieved. CO2-rich RegBS can obtain the higher EC50 value than RBS, indicating higher application concentrations in agriculture/horticulture, and hence lower phytotoxicities to crops and plants. The maximum EC50 value with 168.8 mL/L can be achieved when using the CO2-rich RegBS obtained at 47 °C and 10 kPa. 77 °C and 40 kPa may be the optimum conditions to recover CO2 absorption performance of RBS in terms of higher CO2 reabsorption performance and lower phytoxicity of CO2-rich RegBS. © 2017 Elsevier Ltd
Zhang X.,Huazhong Agricultural University |
Zhang X.,The Cooperative Innovation Center for Sustainable Pig Production |
Liu X.,Huazhong Agricultural University |
Liu X.,Yantai University |
And 6 more authors.
Oncotarget | Year: 2017
Caffeine, as an oocyte aging inhibitor, was used in many different species to control or delay oocyte aging. However, the safety of caffeine and developmental competence of aged oocytes inhibited by caffeine has not been studied systematically. So we detected the spindle morphology, distribution of cortical granules, zona pellucida hardening and pronucleus formation to assess oocyte quality of caffeine treated oocytes. We found that aged oocytes treated by caffeine maintained weak susceptibility to activating stimuli and regained normal competent after aged further 6 hr. Caffeine maintained the spindle morphology, changed cortical granules distribution of aged oocytes and could not prevent zona pellucida hardening. Furthermore, caffeine increased pronucleus formation of aged oocytes and decreased fragmentation after fertilization. These results suggested that caffeine could maintain the quality of aged oocytes safely in mouse.
Wei X.,Tibet Agricultural and Animal Husbandry College |
Wei X.,Huazhong Agricultural University |
Wang Y.,Huazhong Agricultural University |
Luo R.,Tibet Agricultural and Animal Husbandry College |
And 4 more authors.
Developmental and Comparative Immunology | Year: 2017
The Pasteurella multocida lipoprotein B (PlpB) was cloned from Pasteurella multocida (P. multocida) strain LZ-PM (serotype A) and expressed in Escherichia coli (E. coli). Sequence analysis showed that PlpB from different strains of P. multocida exhibited 80.8–99.4% sequence identity to each other, suggesting that PlpB might serve as a cross-protective antigen. The purified PCR product of PlpB gene consisting of 831 base pairs was inserted into the pET-32a (+) plasmid, and then transferred into E. coli. The protective immunity conferred by recombinant PlpB (rPlpB) on mice was evaluated. The results showed that mice immunized with 200 μg of purified rPlpB were protected (60% survival rate) against challenge infection with 1 MLD of P. multocida strain LZ-PM. In conclusion, our data indicated that the PlpB protein may be a potential target as a candidate subunit vaccine for P. multocida infection. © 2017 Elsevier Ltd
Luo H.,Huazhong Agricultural University |
Luo H.,Newhope Liuhe Co. |
Zhou Y.,Huazhong Agricultural University |
Hu X.,Huazhong Agricultural University |
And 5 more authors.
Cell Cycle | Year: 2015
PPARγ and Wnt signaling are central positive and negative regulators of adipogenesis, respectively. Here we identified that, eicosapentaenoic acid (EPA) could effectively induce the transdifferentiation of myoblasts into adipocytes through modulation of both PPARγ expression and Wnt signaling. During the early stage of transdifferentiation, EPA activates PPARγ and PPARγ1, which in turn targets β-catenin to degradation and down-regulates Wnt/β-catenin signaling, such that the myogenic fate of myoblasts could be switched to adipogenesis. In addition, EPA up-regulates the expression of PPARγ1 by activating RXRα, then PPARγ1 binds to the functional peroxisome proliferator responsive element (PPRE) in the promoter of adipocyte-specific PPARγ2 to continuously activate the expression of PPARγ2 throughout the transdifferentiation process. Our data indicated that EPA acts as a dual-function stimulator of adipogenesis that both inhibits Wnt signaling and induces PPARγ2 expression to facilitate the transdifferentiation program, and the transcriptional activation of PPARγ2 by PPARγ1 is not only the key factor for the transdifferentiation of myoblasts to adipocytes, but also the crucial evidence for successful transdifferentiation. The present findings provided insight for the first time as to how EPA induces the transdifferentiation of myoblasts to adipocytes, but also provide new clues for strategies to prevent and treat some metabolic diseases. © 2015, Taylor & Francis Group, LLC.
PubMed | Huazhong Agricultural University, Hubei Academy of Agricultural Science and The Cooperative Innovation Center for Sustainable Pig Production
Type: | Journal: Scientific reports | Year: 2016
Stress response is tightly linked to meat quality. The current understanding of the intrinsic mechanism of meat deterioration under stress is limited. Here, male piglets were randomly assigned to cortisol and control groups. Our results showed that when serum cortisol level was significantly increased, the meat color at 1h postmortem, muscle bundle ratio, apoptosis rate, and gene expression levels of calcium channel and cell apoptosis including SERCA1, IP3R1, BAX, Bcl-2, and Caspase-3, were notably increased. However, the value of drip loss at 24h postmortem and serum CK were significantly decreased. Additionally, a large number of differentially expressed genes (DEGs) in GC regulation mechanism were screened out using transcriptome sequencing technology. A total of 223 DEGs were found, including 80 up-regulated genes and 143 down-regulated genes. A total of 204 genes were enriched in GO terms, and 140 genes annotated into in KEGG database. Numerous genes were primarily involved in defense, inflammatory and wound responses. This study not only identifies important genes and signalling pathways that may affect the meat quality but also offers a reference for breeding and feeding management to provide consumers with better quality pork products.
Nie Y.-F.,Huazhong Agricultural University |
Nie Y.-F.,The Cooperative Innovation Center for Sustainable Pig Production |
Hu J.,Huazhong Agricultural University |
Hu J.,The Cooperative Innovation Center for Sustainable Pig Production |
And 2 more authors.
Journal of Zhejiang University: Science B | Year: 2015
Bile acid (BA) is de novo synthesized exclusively in the liver and has direct or indirect antimicrobial effects. On the other hand, the composition and size of the BA pool can be altered by intestinal microbiota via the biotransformation of primary BAs to secondary BAs, and subsequently regulate the nuclear farnesoid X receptor (FXR; NR1H4). The BA-activated FXR plays important roles in BA synthesis and metabolism, glucose and lipid metabolism, and even hepatic autophagy. BAs can also play a role in the interplays among intestinal microbes. In this review, we mainly discuss the interactions between BAs and intestinal microbiota and their roles in regulating host metabolism, and probably the autophagic signaling pathway. © 2015, Zhejiang University and Springer-Verlag Berlin Heidelberg.
Huang C.,Huazhong Agricultural University |
Huang C.,The Cooperative Innovation Center for Sustainable Pig Production |
Geng J.,Huazhong Agricultural University |
Geng J.,The Cooperative Innovation Center for Sustainable Pig Production |
And 2 more authors.
Cell and Tissue Research | Year: 2016
Mesenchymal stem cells (MSCs), also referred to as multipotent stromal cells, have been isolated from various adult tissue sources because of their capabilities of differentiating into multiple cell lineages including osteoblasts, thus providing a novel approach for treating bone diseases and metabolic disorders. Despite extensive potential in cell therapy and widespread interest in clinical applications of MSCs, the molecular mechanisms with regard to the regulation of their therapeutic properties and osteoblast differentiation remain to be fully elucidated. MicroRNAs (miRNAs), a novel class of endogenous small noncoding RNAs, regulate gene expressions by translational repression or degradation of their targets. Recently, emerging evidence has shown that miRNAs are closely involved in controlling the key steps of osteoblast differentiation in MSCs. This review focuses on miRNAs and their roles in regulating osteogenic differentiation of MSCs. © 2016 Springer-Verlag Berlin Heidelberg
Tan C.,Huazhong Agricultural University |
Wei H.,Huazhong Agricultural University |
Ao J.,Huazhong Agricultural University |
Long G.,Huazhong Agricultural University |
And 2 more authors.
Applied and Environmental Microbiology | Year: 2016
Although dietary fibers contribute to health and physiology primarily via the fermentative actions of the gut microbiota of the hosts, few studies have focused on how these interactions influence the metabolic status of sows. Here, the effects of inclusion of konjac flour (KF) in a gestation diet on oxidative stress status, insulin sensitivity, and gut microbiota were investigated to elucidate the correlation between the microbiota and metabolic changes in sows. Sows were assigned to either control or 2.2% KF dietary treatment during gestation. The gut microbiota population in sows during gestation and lactation was assessed by 16S rRNA gene sequencing. The oxidative stress parameters, homeostasis model assessment (HOMA) values, and fatty acids in the blood of sows were also assessed. Compared to the control diet group, KF significantly reduced the serum levels of reactive oxygen species (ROS) and 8-hydroxy-deoxyguanosine (8-OHdG) but increased the serum concentrations of glutathione peroxidase (GSH-Px) in sows on day 1 in lactation. Additionally, sows in the KF group had a lower HOMA insulin resistance value but a higher HOMA insulin sensitivity (HOMA-IS) value. KF induced changes in the gut microbial composition at the phylum and genus levels. The increased relative abundances of Akkermansia and Roseburia in the KF group were positively correlated with the HOMA-IS. Overall, dietary KF alleviated oxidative stress and improved insulin sensitivity of sows, and the changes in the gut microbiota in response to KF may have been correlated with the host metabolism response.
PubMed | Huazhong Agricultural University and The Cooperative Innovation Center for Sustainable Pig Production
Type: Journal Article | Journal: Gene | Year: 2016
Since October 2010, porcine epidemic diarrhea (PED) caused by variant porcine epidemic diarrhea virus (PEDV) has led great economic losses to the global pig industry, especially in China. To study the genetic characteristics of PEDV strains in Chinese mainland, a total of 603 clinical samples from nine provinces/districts of Chinese mainland from January 2014 to December 2015 were collected for RT-PCR detection and 1-1323bp of S gene of 91 isolates and ORF3 gene of 46 isolates were sequenced. The results showed that the variant PEDV were the dominant pathogens of viral diarrhea diseases in these areas. Six novel variant PEDV strains (FJAX1, FJAX2, HeNPDS1, HeNPDS2, HeNPY3, and HeNPY4) with two amino acids (aa) deletion at the 56-57 aa of S protein were identified. A total of 405 Chinese PEDV strains were subjected to phylogenetic and phylogeographic analysis. The results revealed that the subgroup Va in variant PEDV group were the dominant subgroup and the spread trend of variant PEDV strains seemed to be from the southeast coastal districts to other coastal districts and interior districts. The N-terminal of S gene (1-750bp), to some extent, could represent S1 or full length S gene for phylogenetic, similarity, antigen index, hydrophilicity plot, and differentiation analyses. The 404-472bp of S gene contained the three genetic markers, i.e., TAA insertion at 404-405bp, ACAGGT deletion at 430-435bp, and ATA deletion at 455-457bp can be used to differentiate the classical and variant virulent parental/attenuated PEDV strains and help us to learn the infectious and genetic characteristics of PEDV strains more convenient and cheaper. This study has important implication for understanding the infectious, genetic, and evolutionary aspects of PEDV strains in Chinese mainland.