The College of Computer Science
Lodz, Poland
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Olkowski B.I.,Siedlce University Of Natural Sciences And Humanities | Janiuk I.,Siedlce University Of Natural Sciences And Humanities | Jakubczak A.,The College of Computer Science
Acta Veterinaria Brno | Year: 2010

This work examined the impact of enzyme preparation with specific activity towards non starch polysaccharides on performance, morphological characteristics of gastrointestinal tract organs, microscopic evaluation of jejunal mucosa, and microbial status of ileum, caeca, and excreta in broilers fed a diet containing a high content of lupine meal. One-day-old chickens (Ross 308, mixed sex) were randomly divided into control and experimental groups. Each group consisted of 36 birds, with 6 replications,and with 6 chickens per replication. The control group was fed the basal diet (consisting of maize and 40% of lupine), while the experimental treatment group was fed the basal diet supplemented with 0.06% commercial enzyme (Ronozyme VP). Chickens were fed diets in mash form for 4 weeks. Enzyme preparation significantly (P < 0.05) improved feed consumption and chicken growth, and slightly improved total tract digestibility of dietary ingredients and energy. Enzyme preparation significantly reduced (P < 0.05) the size of gastrointestinal tract organs and had an impact on jejunal mucous membrane of chickens evidenced by elongation of villi and deepening of crypts. No significant effects of dietary enzyme on counts of the analysed bacteria in the jejunal digesta were observed, but enzyme preparation significantly (P < 0.05) reduced the number of Enterobacteriaceae in caeca and excreta, and coliforms in excreta only (P < 0.01). Appropriate combination of enzyme preparations with activity towards degrading carbohydrates may offer a potential to reduce the deleterious impact of lupine in broilers.

Waszkiewicz N.,Medical University of Bialystok | Chojnowska S.,The College of Computer Science | Zalewska A.,Medical University of Bialystok | Szulc A.,Medical University of Bialystok | Szajda S.D.,Medical University of Bialystok
Drug and Alcohol Dependence | Year: 2013

Background: A sensitive alcohol marker, β-hexosaminidase (HEX), in the saliva of alcoholics, is investigated for the first time. Methods: The activity, specific-activity and output of total HEX and its isoenzymes HEX A and HEX B were measured in the saliva of healthy controls (C), alcohol-dependent non-smokers (ANS), and alcohol-dependent smokers (AS). Results: We observed a significantly increased activity/specific-activity and output of HEX A in the ANS and AS groups, due to the inflammatory state of the oral-cavity/salivary-glands. Significantly increased activity of HEX A contributed to an increase in the salivary activity of the total HEX in the ANS group. A significant decrease in the activity/specific-activity of HEX B in AS seemed to be due to HEX B inactivation by cigarette smoke. We noticed a tendency for deteriorated dental state (lower decayed-missing-filled-teeth index - DMFT), worse periodontal state (higher gingival index - GI and papilla-bleeding index - PBI) in AS, and worse periodontal state (higher GI) in ANS, as compared to the controls. We found no differences in the salivary protein concentrations between all groups and decreased salivary flow in both alcoholic groups as compared to the controls. In alcoholics, the area under the curve (AUC) for HEX A activity/specific-activity was significantly greater than for HEX and HEX B. The salivary HEX A activity/specific-activity had good/excellent sensitivity and specificity in smoking and non-smoking alcoholics, whereas salivary HEX and HEX B had poor/fair sensitivity and specificity. Conclusions: Salivary HEX A may be helpful in the diagnosis of chronic alcohol intoxication, even in smokers. © 2012 Elsevier Ireland Ltd.

Waszkiewicz N.,Medical University of Bialystok | Szajda S.D.,Medical University of Bialystok | Kepka A.,Childrens Memorial Health Institute | Szulc A.,Medical University of Bialystok | Zwierz K.,The College of Computer Science
Biochemical Society Transactions | Year: 2011

Up to 30% of all hospital admissions and health-care costs may be attributable to alcohol abuse. Ethanol, its oxidative metabolites, acetaldehyde and ROS (reactive oxygen species), non-oxidative metabolites of alcohol [e.g. FAEEs (fatty acid ethyl esters)] and the ethanol-water competition mechanism are all involved in the deregulation of glycoconjugate (glycoprotein, glycolipid and proteoglycan) metabolic processes including biosynthesis, modification, transport, secretion, elimination and catabolism. An increasing number of new alcohol biomarkers that are the result of alcohol-induced glycoconjugate metabolic errors have appeared in the literature. Glycoconjugate-related alcohol markers are involved in, or are a product of, altered glycoconjugate metabolism, e.g. CDT (carbohydrate-deficient transferrin), SA (sialic acid), plasma SIJ (SA index of apolipoprotein J), CETP (cholesteryl ester transfer protein), β-HEX (β-hexosaminidase), dolichol, EtG (ethyl glucuronide) etc. Laboratory tests based on changes in glycoconjugate metabolism are useful in settings where the co-operativeness of the patient is impaired (e.g. driving while intoxicated) or when a history of alcohol use is not available (e.g. after trauma). In clinical practice, glycoconjugate markers of alcohol use/abuse let us distinguish alcoholic from non-alcoholic tissue damage, having important implications for the treatment and management of diseases. ©The Authors Journal compilation © 2011 Biochemical Society.

Chojnowska S.,The College of Computer Science | Kepka A.,Childrens Memorial Health Institute | Szajda S.D.,Medical University of Bialystok | Waszkiewicz N.,Medical University of Bialystok | And 2 more authors.
Biochemical Society Transactions | Year: 2011

Exoglycosidases are hydrolases involved in lysosomal degradation of oligosaccharide chains of glycoconjugates (glycoproteins, glycolipids and proteoglycans). In tissues and body fluids, a higher exoglycosidase specific activity is found in N-acetyl-β-hexosaminidase, than β-glucuronidase, α-L-fucosidase, β-galactosidase, α-mannosidase and α-glucosidase. Determination of exoglycosidases (especially N-acetyl-β-hexosaminidase and β-glucuronidase) in body fluids could be an inexpensive, easy to perform and sensitive test for pathological evaluation, as well as in screening and monitoring many diseases, including alcohol abuse, risk of arteriosclerosis, bacterial infections (e.g. Lyme borreliosis), chronic inflammatory processes, such as rheumatoid arthritis and juvenile idiopathic arthritis, asthma, autoimmune hepatitis and primary biliary cirrhosis, as well as cancers. © The Authors Journal compilation ©2011 Biochemical Society.

Szweda P.,Technical University of Gdansk | Schielmann M.,Technical University of Gdansk | Milewski S.,Technical University of Gdansk | Frankowska A.,Siedlce University Of Natural Sciences And Humanities | Jakubczak A.,The College of Computer Science
Polish Journal of Microbiology | Year: 2012

The aim of the study was phenotypic and genotypic analysis of 132 S. aureus strains isolated from mastitis in eastern Poland in respect to their biofilm formation ability. The analysis of the size polymorphism of fragment X in the gene encoding protein A (spa) revealed high genetic differentiation of the analyzed group of isolates. The ability of biofilm formation by the isolates was tested using two phenotypic methods. The Congo Red plate assay was found to be irreproducible and very subjective. More objective results were obtained using the spectrophotometric, microtiter plate assay. Most of the isolates, namely 76/132 (57.6%) were classified as biofilm producers depending on the value of absorbance in the microtiter plate test. All of the isolates tested were found to possess both icaA and icaD genes, while the bap gene was absent in all strains.

Szajda S.D.,Medical University of Bialystok | Waszkiewicz N.,Medical University of Bialystok | Chojnowska S.,The College of Computer Science | Zwierz K.,The College of Computer Science
Biochemical Society Transactions | Year: 2011

Pancreatic cancer is the fourth most common cause of death from cancer in the world and the sixth in Europe. Pancreatic cancer is more frequent in males than females. Worldwide, following diagnosis of pancreatic cancer, <2% of patients survive for 5 years, 8% survive for 2 years and <50% survive for only approx. 3 months. The biggest risk factor in pancreatic cancer is age, with a peak of morbidity at 65 years. Difficulty in the diagnosis of pancreatic cancer causes a delay in its detection. It is one of the most difficult cancers to diagnose and therefore to treat successfully. Additional detection of carbohydrate markers may offer a better diagnosis of pancreatic cancer. Carbohydrate markers of cancer may be produced by the cancer itself or by the body in response to cancer, whose presence in body fluids suggests the presence and growth of the cancer. The most widely used, and best-recognized, carbohydrate marker of pancreatic cancer is CA 19-9 [CA (carbohydrate antigen) 19-9]. However, the relatively non-specific nature of CA 19-9 limits its routine use in the early diagnosis of pancreatic cancer, but it may be useful in monitoring treatment of pancreatic cancer (e.g. the effectiveness of chemotherapy), as a complement to other diagnostic methods. Some other carbohydrate markers of pancreatic cancer may be considered, such as CEA (carcinoembryonic antigen), CA 50 and CA 242, and the mucins MUC1, MUC2 and MUC5AC, but enzymes involved in the processing of glycoconjugates could also be involved. Our preliminary research shows that the activity of lysosomal exoglycosidases, including HEX (N-acetyl-β-D-hexosaminidase), GAL (β-D-galactosidase), FUC (α-L-fucosidase) and MAN (α-D-mannosidase), in serum and urine may be used in the diagnosis of pancreatic cancer. © The Authors Journal compilation ©2011 Biochemical Society.

The paper presents the conductivity of the ground effect on the distribution of temperature field in three-phasehighvoltage cables, 64/110 kV, depending on the depth of their arrangement in the ground. The simulation uses the finite element method FEM.

Krzych L.J.,Medical University of Silesia, Katowice | Krzych L.J.,The College of Computer Science | Ratajczyk D.,Bar Association in Katowice
Journal of Forensic and Legal Medicine | Year: 2013

Background Knowledge of patients' rights is obligatory to create a partner-based relationship between the doctor and the patient. Therefore, we sought to evaluate the awareness of patients' rights by subjects admitted to a multidisciplinary university hospital in Poland. Methods This project was cross-sectional in design and conducted between 06.2012 and 09.2012. The study group comprised 275 persons aged 57.4 ± 15.3 years. A 21-item questionnaire based on legal regulations on patients' rights was applied. Results Over 80% of respondents were aware of their right to choose a treating physician, refusal of the proposed treatment, the choice of the place where the patient is treated, the right of access to medical records, free meals, pastoral care, ability to provide to third parties information about the state of health, as well as giving information to particular persons by phone. The least awareness was shown in relation to the form of giving consent (42.9%) or refusal of consent (50.5%) to treatment and the doctors' right to refuse the presence of a person close to the patient during treatment (16.4%). Factors of significant impact on the findings were participants' age and education level. Conclusions Patients are characterized by a considerable awareness of their rights. From the point of view of health care system, the most important is knowledge of the laws related to the broad access to medical services, including choice of attending physician but also to obtain assistance in emergency situations of life and health care in each facility health, regardless of place of residence. © 2013 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.

Tykocki J.,The College of Computer Science | Jordan A.,The College of Computer Science
Przeglad Elektrotechniczny | Year: 2014

The paper presents the "Pareto Principle"used for the analysis of a multi-parameter temperature field in a single phase high voltage cable core of 110kV. The study makes use of a multi-criteria Pareto model and ABC charts to determine the qualitative and quantitative impact of some specific parameters on the temperature of the cable core. © 2014, Wydawnictwo SIGMA - N O T Sp. z o.o. All rights reserved.

Stachelska M.A.,The College of Computer Science
Acta Scientiarum Polonorum, Technologia Alimentaria | Year: 2015

Background: The aim of this paper was to assess the inhibitory properties of salts of phenolic acids against Escherichia coli O157:H7 ATCC 8739. Escherichia coli O157:H7 is a pathogen which is able to produce verotoxins provoking hemorrhagic diarrhea in humans. There is a strong need for the effective natural methods eliminating E. coli O157:H7 from food. Methodology: The following salts were tested: sodium, potassium and lithium salts of ortho-coumaric, meta-coumaric and para-coumaric acids. The 1%, 2%, 3%, 4% and 5% water solutions of each substance were prepared. Agar-well diffusion method was applied. Petri dishes were incubated at 35°C for 24 h. At the end of the incubation period, inhibition zones which appeared on the medium Petri dishes were calculated in millimeters. Results: It was found that lithium salt of o-coumaric acid, potassium salt of o-coumaric acid, lithium salt of m-coumaric acid and sodium salt of m-coumaric acid were most effective towards E. coli O157:H7, while potassium salt of m-coumaric acid, a sodium salt of p-coumaric acid were slightly less effective and lithium salt of p-coumaric acid did not possess any antimicrobial activity. Conclusion: The salts of phenolic acids having various structural features showed different characteristics towards foodborne pathogens. Such findings indicate that phenolic acids and their salts may be a potential bio-alternative for chemical food preservation. © by Wydawnictwo Uniwersytetu Przyrodniczego w Poznaniu.

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