The Children Hospital of Wuhan

Wuhan, China

The Children Hospital of Wuhan

Wuhan, China
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Zhou S.,The Children Hospital of Wuhan | Zhang X.,The Traditional Chinese Medicine Hospital of Jiangxia | Duan X.,The Children Hospital of Wuhan | Mei H.,The Children Hospital of Wuhan
Biomedical Research (India) | Year: 2015

To establish a method for determination of glycyrrhizic and glycyrrhetinic acids in Weiyanning Granule. The contents of glycyrrhizic and glycyrrhetinic acids are determined by HPLC under conditions as follows: Diamonsil C18 (4.6 mm × 250 mm, 5 μm) column, mobile phase of acetonitrile- 0.05 mol/L ammonium acetate solution (A: 0.05 mol/L ammonium acetate solution; B: acetonitrile, gradient elution: 0~30 min: 32% B; 30~60 min: 75% B); flow rate of 1.0 ml/min; and detection wavelength of 250 nm. The determined linear range of glycyrrhizic acid is 0.320~1.920 μg, and average recovery is 98.6%, with RSD = 1.98%; linear range of glycyrrhetinic acid is 0.039~0.234 μg, and average recovery is 97.3%, with RSD = 3.20%. The method established in this paper is simple, accurate and reproducible, which provides a new way for the quality control of Weiyanning Granule. © 2015, Biomed Res-India. All rights reserved.


Duan X.,The Children Hospital of Wuhan | Ye G.,The Children Hospital of Wuhan | Bian H.,The Children Hospital of Wuhan | Yang J.,The Children Hospital of Wuhan | And 7 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

To investigate the management of Meckel’s diverticulum in children and the feasibility of using laparoscopic and laparoscopically assisted Meckel’s diverticulum resection and intestinal anastomosis according to the different subtypes classified laparoscopically. 55 symptomatic Meckel’s diverticulum cases were classified into two categories, the simple and the complex types depending on Meckel’s diverticulum appearance upon laparoscopic exploration. Forty-one cases of simple Meckel’s diverticulum were treated with simple diverticulectomy during laparoscopy, and 14 cases of complicated Meckel’s diverticulum were treated with laparoscopically assisted Meckel’s diverticulum resection and intestinal anastomosis. The operation time for the laparoscopically assisted was significant longer than laparoscopic-only surgeries [45~123 min (54.57 ± 20.17min) vs 29~78min (38.85 ± 9.75 min)], P = 0.013. Among the 55 cases, Just one child with simple type MD during laparoscopic exploration, and presented a diverticulum with a base that was considered to be in the mesangial margin. The remaining 54 patients were cured, and follow-up for 4~36 months revealed that they did not present abdominal pain, and no hematochezia occurred as a complication. Surgery selection either laparoscopy only or transumbilical laparoscopically assisted intestinal resection and intestinal anastomosis by laparoscopic exploration for Meckel’s diverticulum treatment, based on the type of Meckel’s diverticulum in children, is safe, feasible, and effective. © 2015, E-Century Publishing Corporation. All Rights Reserved.

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