The Central Hospital of Zibo
The Central Hospital of Zibo
Li L.,Shandong University |
Li L.,Peoples Hospital Of Rizhao City |
Liu Y.,Hospital of Traditional Chinese Medicine of Rizhao City |
Yu N.,Peoples Hospital Of Rizhao City |
And 4 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2017
Objective: To study the risk factors of placenta praevia and its influence on maternal and perinatal outcomes. Methods: a retrospective analysis of 260 cases of patients with pernicious placenta praevia (PPP) in Qilu Hospital of Shandong University and Shandong Rizhao People’s Hospital from January 2012 to December 2015 was processed. Among them, 118 cases of patients with placenta attached to the uterine cesarean scar were assigned as PPP group, while the remaining 142 patients were assigned as ordinary placenta praevia (OPP) group. We selected 120 healthy pregnant women in the same period in our hospital as control group. The clinical data and perinatal outcomes of the three groups were compared. Results: the pregnancy complications and neonatal outcomes of PPP group were worse than that in OPP group and the control group (P<0.05). Multivariate logistic analysis showed age (≥35 years old, OR: 2.263, 95.0% CI: 1.346-4.749), parity (2 times, OR: 1.826, 95.0% CI: 1.162-3.013; ≥3 times, OR: 4.692, 95.0% CI: 1.737-5.845), abortion (1 time, OR: 1.427, 95.0% CI: 0.723-2.136; ≥2 times, OR: 3.648, 95.0% CI: 1.827-5.656), previous cesarean section (≥2 times, OR: 2.783, 95.0% CI: 1.562-4.918) were risk factors for PPP. Conclusion: single factor analysis showed that the number of cesarean section, abortion, pregnancy number and age were the independent risk factors for R. Reasonable assessment of treatment options, timely termination of pregnancy, as well as adequate preoperative evaluation of patients for reducing complications and uterine resection ratio are necessary to effectively improve the pregnancy outcomes. © 2017, E-Century Publishing Corporation. All rights reserved.
Wang F.,The Central Hospital of Zibo |
Zhang R.,The Shouguang Peoples Hospital |
Cui Y.,The Shouguang Peoples Hospital |
Sheng L.,The Shouguang Peoples Hospital |
And 4 more authors.
Research on Chemical Intermediates | Year: 2017
Abstract: A series of new 3,4-dihydronaphthalen-1(2H)-one derivatives (6a–f) were designed, and synthesized by the Claisen–Schmidt condensation reaction. Their structures were characterized by NMR, FTIR, and MS spectroscopy. Their antitumor activities against human neoplastic cell lines Hela, Hepg2, K562, THP-1, SW1990, MIA PaCa-2, NCI-H460 and SK-BR-3 by the MTT method exhibited obvious anticancer activities and their cytotoxicities for LO2 cell lines were lower than DOX, especially for 6b and 6d. The inhibition activities against the Bcl-2 protein for 6b was evaluated and the result shows that lipophilic 3,4-dimethoxybenzylidene and 3,4-dihydronaphthalen-1(2H)-one could bind slightly to the active pockets of the Bcl-2 protein. Graphical Abstract: [Figure not available: see fulltext.] © 2017 Springer Science+Business Media Dordrecht
Qiao-feng W.,Chengdu University of Technology |
Ling-ling G.,The Central Hospital of Zibo |
Shu-guang Y.,Chengdu University of Technology |
Sheng-feng L.,Chengdu University of Technology |
And 3 more authors.
Experimental Gerontology | Year: 2011
A 1H NMR-based metabonomic method was used to investigate the metabolic change of plasma in senescence-prone 8 (SAMP8) mice before and after electro-acupuncture (EA). Sixteen SAMP8 male mice (aged 8months) were randomly divided into model group and acupuncture treatment group while the later group received EA treatment for 21days. Eight senescence-resistant 1 (SAMR1) mice were used as the control group. Morris water maze was used to evaluate the effects of EA. All mice plasma samples obtained from different groups were analyzed by using 600MHz 1H nuclear magnetic resonances ( 1H NMR) spectroscopy. The data sets were analyzed by Principal Components Analysis (PCA) and Partial Least Squares-Discriminant Analysis (PLS-DA) to discriminate the key plasma metabolites among different groups. Results indicated that both the escape and probe tasks of SAMP8 could be improved by EA treatment. Metabonomic study showed that SAMR1 and SAMP8 were separated clearly in both CPMG_OSC_PLS and LED _OSC_PLS score plots. Interestingly, samples obtained from EA group were distributed closely to SAMR1 group in CPMG_OSC_PLS score plot, but away from SAMP8 group in LED_OSC_PLS score plot. Corresponding loading plots showed that much less lactate was seen in SAMP8 mice plasma. Other changes including higher levels of dimethylamine (DMA) Choline and α-glucose but lower levels of leucine/isoleucine, HDL, LDL/VLDL, 3-Hydroxybutyrate (3-HB), and Trimethylamine N-oxide (TMAO) were observed in the SAMP8 mice plasma than in the SAMR1. After EA treatment, the levels of lactate, DMA, choline and TMAO were improved. Results of this work can provide valuable clues to the understanding of the metabolic changes in the senile impairment of mice. It is also hoped that the methodology can be used in evaluating the effects of EA and understanding the underlying acupuncture mechanism in treating neurodegenerative diseases. © 2011 Elsevier Inc.
PubMed | Sun Yat Sen University and The Central Hospital of Zibo
Type: Journal Article | Journal: International journal of molecular medicine | Year: 2014
Breast cancer (BC) is a leading cause of cancer-related mortality in females and is recognized as a molecularly heterogeneous disease. Previous studies have suggested that alternative messenger RNA (mRNA) processing, particularly alternative polyadenylation [poly(A)] (APA), can be a powerful molecular biomarker with prognostic potential. Therefore, in the present study, we profiled APA sites in the luminal B subtype of BC by sequencing APA sites (SAPAS) method, in order to assess the relation of these APA site-switching events to the recognized molecular subtypes of BC, and to discover novel candidate genes and pathways in BC. Through comprehensive analysis, the trend of APA site-switching events in the 3 untranslated regions (3UTRs) in the luminal B subtype of BC were found to be the same as that in MCF7 cell lines. Among the genes involved in the events, a significantly greater number of genes was found with shortened 3UTRs in the samples, which were samples of primary cancer with relatively low proliferation. These findings may provide novel information for the clinical diagnosis and prognosis on a molecular level. Several potential biomarkers with significantly differential tandem 3UTRs and expression were found and validated. The related biological progresses and pathways involved were partly confirmed by other studies. In conclusion, this study provides new insight into the diagnosis and prognosis of BC from the APA site profile aspect.
Zhang L.,Soochow University of China |
Li J.,The Central Hospital of Zibo |
Yang H.,Soochow University of China |
Luo Z.,Soochow University of China |
Zou J.,Soochow University of China
Oncology Letters | Year: 2012
Percutaneous vertebroplasty (PVP) or percutaneous kyphoplasty (PKP) is utilized for the treatment of vertebral compression fractures (VCFs). The present study was conducted to evaluate biopsy results obtained from VCFs during PVP or PKP. Between January 2003 and December 2011, 692 vertebral body biopsies were obtained from 546 patients (442 females and 104 males; mean age, 72.3 years), who underwent PVP or PKP for treatment of VCFs. A history of malignancy was identified in 44 patients. Histological/immunohistochemical evaluations were performed by experienced pathologists and 89.9% of biopsies obtained from 546 patients were suitable for histological/immunohistochemical evaluation. The biopsy results of 398 patients were in good agreement with the diagnosis of osteoporotic VCFs. Among the 44 patients with a history of malignancy, malignancy was identified in 25 patients, while osteoporosis was identified in 16 patients. Biopsies of the other 3 patients were not suitable for pathological examination. There were only 2 patients with previously undiagnosed malignancy. One patient was diagnosed with multiple myeloma, while another patient was diagnosed with metastatic carcinoma. In the present study, the rate of unsuspected malignancy was 0.4%. No symptomatic complications occurred as a result of biopsy. The results indicate that biopsy is safely performed during PVP or PKP and it is an efficacious procedure that verifies the pathological process and assists in identifying previously unsuspected processes responsible for VCFs. We recommend obtaining the biopsy of every collapsed vertebral body during PVP or PKP procedures.
Wei C.,The Central Hospital of Zibo |
Han D.-F.,The Central Hospital of Zibo |
Li T.,The Central Hospital of Zibo
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: A large number of literatures have shown that surgeons expose to a higher radiation dose during puncture and bone cement injection in percutaneous vertebral augmentation. OBJECTIVE: To review the research progress in radiation doses and safeguard procedures in percutaneous vertebral augmentation. METHODS: By using “percutaneous vertebral augmentation, radiation doses, radiation protective” as key words, we retrieved articles related to radiological protection during percutaneous vertebral augmentation published from January 1995 to December 2014 in Wangfang database and PubMed database. RESULTS AND CONCLUSION: One basic principle of radiation protection is that the radiation dose decreases rapidly with distance from the radioactive sources. Surgeons should maximize the distance from the radioactive sources as far as possible in case there is no effect on the operation. To optimize the setting and position of C-arm machine, wear protective devices and put lead shields as well as computer navigation and radiation training in surgeons all can help to reduce the radiation doses during percutaneous vertebral augmentation. Remote bone cement injection device is easy to control but not increases the radiation doses. In addition, the influence of surgery bed on radiation dose remains to be further studied. We believe that with the in-depth research on radiological protection, vertebral augmentation technique will be safer in clinical application. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
PubMed | Shandong University and The Central Hospital of Zibo
Type: Journal Article | Journal: Pharmacological reports : PR | Year: 2016
To assess the effects of the poly (ADP-ribose) polymerase-1 (PARP-1) inhibitor PJ34 and ERK1/2 inhibitor U0126 on the proliferation and epithelial mesenchymal transitions (EMT) of cisplatin resistant ovarian cancer SKOV-3 cells.Proliferation of SKOV-3 cells was evaluated using a 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide assay with PJ34 and U0126 treatment. Expression changes of E-cadherin and vimentin with PJ34 and U0126 treatment was examined using Western blot and quantitative PCR. In addition, invasion assay was performed in cells treated with PJ34 and U0126.PJ34 and U0126 inhibited proliferation of SKOV-3 cells in a time dependent manner. PJ34 and U0126 suppressed the expression of vimentin and enhanced the expression of E-cadherin. PJ34 and U0126 reduced cell invasion. The inhibitory effects of PJ34 and U0126 were stronger than PJ34 alone. PJ34 inhibited the proliferation and invasion of SKOV-3 cells which can be enhanced by ERK1/2 inhibitor U0126.These inhibitory effects are partially due to PARP-1 and ERK1/2 mediated attenuation of EMT activity.