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Lan X.,The Central Hospital of Enshi Autonomous Prefecture | Lan T.,Hubei University | Faxiang Q.,The Central Hospital of Enshi Autonomous Prefecture
International Journal of Clinical and Experimental Medicine | Year: 2015

Background: Interleukin-10 (IL-10) is a multifunctional cytokine with both immunosuppressive and antiangiogenic properties and it plays an important role in the pathogenesis of cancer. A number of studies have examined the association between its promoter -1082/-819/-592 polymorphism and risk of lung cancer. However, the results are inconsistent and inconclusive. The aim of this study was to explore whether the IL-10 gene polymorphism contribute to the susceptibility of lung cancer. Method: We searched in PubMed, EMBASE, Cochrane Library as well as Chinese databases including China National Knowledge Infrastructure (CNKI) and Wan Fang database for all the relevant studies up to May 15, 2015. The data were extracted by two independent authors. The pooled odds ratios (ORs) with 95% confidence intervals (CIs) under co-dominant model, dominant model and recessive model were estimated. Results: A total of 8 studies involving 2033 cases and 3100 controls were included in the meta-analysis. The results revealed that the IL-10 -592C/A polymorphism was related to lung cancer susceptibility under all models (C allele vs. A allele: OR=1.195, 95% CI=1.075-1.329; CC vs. AA: OR=1.651, 95%=1.290-2.113; CA vs. AA: OR=1.229, 95%=1.029-1.468; CA+AA vs. CC: OR=0.832, 95%=0.704-0.984; CC+CA vs. AA: OR=1.301, 95%=1.100-1.538) and IL-10 -819C/T polymorphism was associated with lung cancer susceptibility under three models (C allele vs. T allele: OR=1.441, 95% CI=1.228-1.691; CC vs. TT: OR=2.444, 95%=1.732-3.449; CC+CT vs. TT: OR=1.496, 95%=1.172-1.908). For IL-10 -1082G/A, there was no significant association between its polymorphism and lung cancer risk. Conclusions: This meta-analysis demonstrated that two polymorphisms (-592C/A and -819C/T) in the promoter region of IL-10 gene were significantly associated with the risk of lung cancer in general population, while -1082G/A polymorphism did not affect susceptibility to lung cancer. © 2015, E-Century Publishing Corporation. All rights reserved.


Wang Y.-L.,The Central Hospital of Enshi Autonomous Prefecture | Li T.,The Central Hospital of Enshi Autonomous Prefecture | Li J.-Z.,The Central Hospital of Enshi Autonomous Prefecture | Wu Q.-S.,The Central Hospital of Enshi Autonomous Prefecture
International Eye Science | Year: 2016

AIM:To investigate the effect of mitomycin C combined with complex trabeculectomy in treatment of refractory glaucoma. METHODS:One hundred and ten cases (122 eyes) in our hospital from January 2014 to June 2015 were divided into observation group (55 cases with 62 eyes) and control group (55 cases with 60 eyes). All patients were treated with complex trabeculectomy, and patients in observation group were given mitomycin C. Before and at postoperatively 12mo, the intraocular pressure and visual acuity were examined. Before and at postoperatively 3mo, the levels of Vitamin B12(VB12), Vitamin B6(VB6), folic acid (FA), interleukin-2 (IL-2) and interleukin-6 (IL-6) in peripheral blood were detected by enzyme-linked immunosorbent assay(ELISA). RESULTS:Preoperatively, there were no significant differences on intraocular pressure, visual acuity, VB12, VB6, FA, IL-2, IL-6 between the two groups (P>0.05). At postoperatively 1wk~12mo, the intraocular pressure of observation group was significantly lower than those of control group (P<0.01), the visual acuity was higher than those of control group (P<0.01); VB12, FA, IL-2 and IL-6 of observation group were significantly higher than those of control group (P<0.05) at postoperatively 3mo. CONCLUSION:Mitomycin C combined with complex trabeculectomy in treatment of refractory glaucoma could control intraocular pressure, improve visual acuity, and enhance the operation success rate. Copyright 2016 by the IJO Press.


Chen H.-B.,The Central Hospital of Enshi Autonomous Prefecture | Jiang K.-H.,The Central Hospital of Enshi Autonomous Prefecture | Hu X.-H.,The Central Hospital of Enshi Autonomous Prefecture
Journal of Practical Oncology | Year: 2015

Objective: To investigate the effects of Akt inhibitor perifosine on the proliferation and migration of human prostate cancer DU145 cells. Methods: Human prostate cancer DU145 cells were treated with different concentrations of individual perifosine and/or docetaxel in vitro. Cell proliferation was observed by CCK-8 assay, and cell cycle was detected by flow cytometry (FCM). Wound healing test was used to measure the migration ability of DU145 cells. Results: CCK-8 result indicated that perifosine inhibited the proliferation of DU145 cells in a dose- and time-dependent manner (P<0.01). The inhibition rate increased significantly when cells were treated with perifosine in combination with docetaxel (P<0.01) and perifosine had synergistic effects with docetaxel (combination index<1). Flow cytometry showed that perifosine blocked cell cycle in G2/M stage (P<0.05), and reduced the number of cells in G0/G1 stage (P<0.05). The migration capacity of perifosine-treated cells decreased compared with that of control cells (P<0.05). Conclusion: Perifosine can inhibit the proliferation and migration ability of DU145 cells significantly, which is synergistic with docetaxel. ©, 2015, Journal of Practical Oncology, Editorial Board. All right reserved.


PubMed | The Central Hospital of Enshi Autonomous Prefecture and Wuhan University
Type: | Journal: Molecular and cellular endocrinology | Year: 2016

Previous study has shown that curcumin directly or indirectly suppresses insulin signaling in 3T3-L1 adipocytes. However, the underlying mechanism remains unclear. Here we experimentally demonstrate that curcumin inhibited the ubiquitin-proteasome system (UPS) function, activated autophagy, and reduced protein levels of protein kinase B (Akt) in a dose- and time-dependent manner in 3T3-L1 adipocytes, accompanied with attenuation of insulin-stimulated Akt phosphorylation, plasma membrane translocation of glucose transporter type 4 (GLUT4), and glucose uptake. These invitro inhibitory effects of curcumin on Akt protein expression and insulin action were reversed by pharmacological and genetic inhibition of autophagy but not by inhibition of the UPS and caspases. In addition, Akt reduction in adipose tissues of mice treated with curcumin could be recovered by administration of autophagy inhibitor bafilomycin A1 (BFA). This new finding provides a novel mechanism by which curcumin induces insulin resistance in adipocytes.


PubMed | the Central Hospital of Enshi Autonomous Prefecture and Tianjin Medical University
Type: | Journal: Neuroscience letters | Year: 2016

Anesthetic cardioprotection reduces myocardial infarct size following ischemia-reperfusion injury. However, the underlying mechanisms that drive ischemia-reperfusion injury in cardiomyocytes remain unclear. In this study, we report that isoflurane, a commonly used inhaled anesthetic, can protect cardiomyocytes from anoxia/reoxygenation injury by a nucleotide binding oligomerization domain containing 2 (NOD2)-dependent mechanism. The results showed that isoflurane increased cell viability, and decreased autophagosome generation in primary cardiomyocytes under anoxia/reoxygenation conditions. In addition, western blot revealed that isoflurane reduces the expression of NOD2. Overexpression of NOD2 is accompanied by an increased expression of autophagy-related genes, decreased cell viability, and enhanced expression of phosphorylation p38-mitogen-activated protein kinase (p38MAPK), while NOD2 knockdown exerted the opposite effect. Following preconditioning with SB203580, a p38MAPK inhibitor, the inhibitory effect of isoflurane on cardiomyocytes autophagy was further enhanced, which suggests that p38MAPK is involved in the mechanism of cardioprotection provided by isoflurane. These findings reveal a novel mechanism underlying isoflurane-afford protection of myocardial injury.


PubMed | The Central Hospital of Enshi Autonomous Prefecture and Tianjin University
Type: | Journal: Oncology reports | Year: 2016

Propofol is one of the most extensively used intravenous anesthetic agents and it can influence the biological behavior of gastric cancer. However, the underlying mechanism is poorly understood. In the present study, we found that propofol significantly inhibited cell proliferation, invasion and migration, and also promoted apoptosis in gastric carcinoma cell lines SGC-7901 and MGC-803, as detected using MTT, colony formation and flow cytometry assays, respectively. Moreover, propofol (10 and 20M) markedly upregulated the expression of inhibitor of growth3 (ING3), which was lower in SGC-7901 and MGC-803 cells compared with that noted in normal human gastric epithelial cell lines GES-1 and HFE145. Furthermore, we transfected SGC-7901 and MGC-803 cells with ING3 overexpression vectors or ING3 small interference RNA (siING3), respectively, to assess the role of ING3 in propofol-induced antitumor activity. The siING3 transfection reversed the effects of propofol on the biological behavior of gastric cancer cells, while transfection of ING3 promoted the effects of propofol. In conclusion, our results indicate that propofol exerts an inhibitory effect on the growth and survival of gastric cancer cells by interfering with ING3 degradation.


PubMed | The Central Hospital of Enshi Autonomous Prefecture and Huazhong University of Science and Technology
Type: | Journal: Oncotarget | Year: 2016

The safety and feasibility of robotic-assisted radical prostatectomy (RARP) compared with retropubic radical prostatectomy(RRP) is debated. Recently, a number of large-scale and high-quality studies have been conducted.To obtain a more valid assessment, we update the meta-analysis of RARP compared with RRP to assessed its safety and feasibility in treatment of prostate cancer.A systematic search of Medline, Embase, Pubmed, and the Cochrane Library was performed to identify studies that compared RARP with RRP. Outcomes of interest included perioperative, pathologic variables and complications.78 studies assessing RARP vs. RRP were included for meta-analysis. Although patients underwent RRP have shorter operative time than RARP (WMD: 39.85 minutes; P < 0.001), patients underwent RARP have less intraoperative blood loss (WMD = -507.67ml; P < 0.001), lower blood transfusion rates (OR = 0.13; P < 0.001), shorter time to remove catheter (WMD = -3.04day; P < 0.001), shorter hospital stay (WMD = -1.62day; P < 0.001), lower PSM rates (OR:0.88; P = 0.04), fewer positive lymph nodes (OR:0.45;P < 0.001), fewer overall complications (OR:0.43; P < 0.001), higher 3- and 12-mo potent recovery rate (OR:3.19;P = 0.02; OR:2.37; P = 0.005, respectively), and lower readmission rate (OR:0.70, P = 0.03). The biochemical recurrence free survival of RARP is better than RRP (OR:1.33, P = 0.04). All the other calculated results are similar between the two groups.Our results indicate that RARP appears to be safe and effective to its counterpart RRP in selected patients.


PubMed | The Central Hospital of Enshi Autonomous Prefecture and Fujian Medical University
Type: Journal Article | Journal: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas | Year: 2014

The physiological mechanisms involved in isoproterenol (ISO)-induced chronic heart failure (CHF) are not fully understood. In this study, we investigated local changes in cardiac aldosterone and its synthase in rats with ISO-induced CHF, and evaluated the effects of treatment with recombinant human brain natriuretic peptide (rhBNP). Sprague-Dawley rats were divided into 4 different groups. Fifty rats received subcutaneous ISO injections to induce CHF and the control group (n=10) received equal volumes of saline. After establishing the rat model, 9 CHF rats received no further treatment, rats in the low-dose group (n=8) received 22.5 g/kg rhBNP and those in the high-dose group (n=8) received 45 g/kg rhBNP daily for 1 month. Cardiac function was assessed by echocardiographic and hemodynamic analysis. Collagen volume fraction (CVF) was determined. Plasma and myocardial aldosterone concentrations were determined using radioimmunoassay. Myocardial aldosterone synthase (CYP11B2) was detected by quantitative real-time PCR. Cardiac function was significantly lower in the CHF group than in the control group (P<0.01), whereas CVF, plasma and myocardial aldosterone, and CYP11B2 transcription were significantly higher than in the control group (P<0.05). Low and high doses of rhBNP significantly improved hemodynamics (P<0.01) and cardiac function (P<0.05) and reduced CVF, plasma and myocardial aldosterone, and CYP11B2 transcription (P<0.05). There were no significant differences between the rhBNP dose groups (P>0.05). Elevated cardiac aldosterone and upregulation of aldosterone synthase expression were detected in rats with ISO-induced CHF. Administration of rhBNP improved hemodynamics and ventricular remodeling and reduced myocardial fibrosis, possibly by downregulating CYP11B2 transcription and reducing myocardial aldosterone synthesis.


PubMed | Maternal and Child Health Hospital of Zigong, The Central Hospital of Enshi Autonomous Prefecture and Huazhong University of Science and Technology
Type: Journal Article | Journal: PloS one | Year: 2016

Ovarian aging is a long-term and complex process associated with a decrease in follicular quantity and quality. The damaging effects of reactive oxygen species (ROS) in ovarian aging and ovarian aging-associated disorders have received relatively little attention. Thus, we assessed if the oxidative stress induced by long-term (defined by the Environmental Protection Agency as at least 30 days in duration) moderate ozone inhalation reduced ovarian reserves, decreased ovarian function and induced ovarian aging-associated disorders. The expression of oxidative stress markers and antioxidant enzymes was used to determine the degree of oxidative stress. Ultrastructural changes in ovarian cells were examined via electron microscopy. The ovarian reserve was assessed by measuring multiple parameters, such as the size of the primordial follicle pool and anti-Mllerian hormone (AMH) expression. The estrous cycle, hormone levels and fertility status were investigated to assess ovarian function. To investigate ovarian aging-associated disorders, we utilized bone density and cardiovascular ultrasonography in mice. The levels of oxidized metabolites, such as 8-hydroxy-2-deoxyguanosine (8-OHdG), 4-hydroxynonenal (4-HNE) and nitrotyrosine (NTY), significantly increased in ovarian cells in response to increased oxidative stress. The ultrastructural analysis indicated that lipid droplet formation and the proportion of mitochondria with damaged membranes in granulosa cells were markedly increased in ozone-exposed mice when compared with the control group. Ozone exposure did not change the size of the primordial follicle pool or anti-Mllerian hormone (AMH) expression. The estrogen concentration remained normal; however, progesterone and testosterone levels decreased. The mice exposed to ozone inhalation exhibited a substantial decrease in fertility and fecundity. No differences were revealed by the bone density or cardiovascular ultrasounds. These findings suggest that the decreased female reproductive function caused by long-term moderate oxidative damage may be due to a decrease in follicle quality and progesterone production.


PubMed | the Central Hospital of Enshi Autonomous Prefecture
Type: Journal Article | Journal: Zhonghua yi xue za zhi | Year: 2016

To investigate the effect of icariin on myocardial hypoxia reoxygenation injury and the possible mechanism.Neonatal Sprague-Dawley rat cardiomyocytes in primary culture were treated with different concentrations of icariin for 24 h prior to hypoxia/reoxygenation injury. Cardiomyocyte apoptosis was evaluated with Tunel staining. The expression levels of apoptosis proteins were detected by Western blotting. The nuclear translocation of p65 was evaluated by immunofluorescence. The p65 signaling pathway was also detected by Western blotting.Myocardial apoptosis rate significantly increased after hypoxia/reoxygenation (control: 1.5% 0.1%;23.4% 1.3%, P<0.05). While icariin significantly reduced cardiomyocyte apoptosis induced by hypoxia/reoxygenation (1 mol/L icariin: 7.2% 0.9%; 10 mol/L icariin: 3.9% 0.8%, both P<0.05). Western blot showed that the expression levels of pro-apoptotic protein, Bax, increased significantly (control: 0.19 0.05;0.41 0.03, P<0.05), while the expression of anti-apoptotic protein, B-Cell CLL/Lymphoma 2 (BCL-2), was significantly reduced (control: 0.15 0.02;0.03 0.01, P<0.05) after hypoxia/reoxygenation. Notably, icariin reduced the expression of Bax (1 mol/L icariin: 0.29 0.01; 10 mol/L icariin: 0.33 0.03, both P<0.05) and increased expression of BCL-2 (1 mol/L icariin: 0.10 0.03; 10 mol/L icariin: 0.11 0.02, both P<0.05). Immunofluorescence showed that NFB-p65 nuclear translocation in cardiomyocytes was increased after hypoxia/reoxygenation (control: 3.6% 0.5%;89.5% 4.8%, P<0.05), while icariin reduced the nuclear translocation of p65 (1 mol/L icariin: 32.6% 2.3%; 10 mol/L icariin: 10.6% 1.0%, both P<0.05). Moreover, icariin reduced the activation of p65 and phosphorylation of IKB induced by hypoxia/reoxygenation in cardiomyocytes.Icariin can protect cardiomyocytes against hypoxia reoxygenation injury, which may be via blocking p65 signaling pathway.

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