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Ruan G.-P.,The Cell Biological Therapy Center | Ruan G.-P.,Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions | Ruan G.-P.,Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province | Yao X.,The Cell Biological Therapy Center | And 11 more authors.
Cellular and Molecular Biology | Year: 2015

The chicken ovalbumin extracts could promote cell survival and proliferation. In the present study, the different components in chicken ovalbumin extracts were further separated to find the component primarily responsible for promoting cell proliferation. Components of differing molecular weight were separated from chicken ovalbumin extracts by ultrafiltration. Different components were co-cultured with different cells at different final concentrations, and the effects on cell proliferation were subsequently determined by a CellTiter 96 Aqueous One Solution Cell Proliferation Assay kit (Promega). Components from chicken ovalbumin extracts less than 3 kD in size can promote 293T cell and 293T-GFP cell proliferation. The components from chicken ovalbumin extract more than 3 kD in size can promote bone marrow or umbilical cord mesenchymal stem cell (MSC) proliferation. The separation of components from chicken ovalbumin less than and more than 3 kD in size that are able to function as active components for the promotion of different cellular proliferation. This discovery may identify a new and convenient additive for cell culture media, promoting cell growth and proliferation. © 2015. Source


Ruan G.-P.,The Cell Biological Therapy Center | Ruan G.-P.,Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions Yunnan Province | Ruan G.-P.,Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province | Yao X.,The Cell Biological Therapy Center | And 20 more authors.
Cytotechnology | Year: 2015

We previously found that chicken egg white extract could promote cell survival and proliferation. In the present study, we further separated this extract into its components to identify those primarily responsible for promoting cell proliferation. Components of differing molecular weight were separated from chicken egg white extract by ultrafiltration and 293T cell cultures were supplemented with various concentrations. The effects on cell proliferation were subsequently determined by a CellTiter 96 Aqueous One Solution Cell Proliferation Assay kit (Promega). We demonstrate that components from chicken egg white smaller than 3 kDa in size are able to function as active ingredients promoting cellular proliferation. This discovery may identify a new and convenient additive for cell culture media to promote cell growth and proliferation. © 2015 Springer Science+Business Media Dordrecht Source


Pan X.-H.,The Cell Biological Therapy Center | Pan X.-H.,Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions Yunnan Province | Pan X.-H.,Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province | Zhu L.,The Cell Biological Therapy Center | And 20 more authors.
Cytotechnology | Year: 2016

The aim of this study was to establish a tree shrew metabolic syndrome model and demonstrate the utility of MSCs in treating metabolic syndrome. We used tree shrew umbilical cord mesenchymal stem cell (TS-UC-MSC) transplantation for the treatment of metabolic syndrome to demonstrate the clinical application of these stem cells and to provide a theoretical basis and reference methods for this treatment. Tree shrew metabolic syndrome model showed significant insulin resistance, high blood sugar, lipid metabolism disorders, and hypertension, consistent with the diagnostic criteria. TS-UC-MSC transplantation at 16 weeks significantly reduced blood sugar and lipid levels, improved insulin resistance and the regulation of insulin secretion, and reduced the expression levels of the pro-inflammatory cytokines IL-1 and IL-6 (P < 0.05). The transplanted TS-UC-MSCs targeted the liver, kidney and pancreas; reduced liver cell degeneration, necrosis, and inflammatory exudation; mitigated bleeding congestion and inflammatory cell infiltration in the kidney; and reduced islet cell degeneration and necrosis. We successfully developed a tree shrew metabolic syndrome model and showed that MSC migrate in diseased organs and can attenuate metabolic syndrome severity in a tree shrew model. © 2016 Springer Science+Business Media Dordrecht Source


Ruan G.-P.,The Cell Biological Therapy Center | Yao X.,The Cell Biological Therapy Center | Liu J.-F.,The Cell Biological Therapy Center | Wang J.-X.,The Cell Biological Therapy Center | And 5 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Systemic lupus erythematosus is an autoimmune disease characterized as an emergence of a variety of auto anti bodies in serum and multi-system and multi-organ lesions. Currently, there is a lack of effective treatment options, and umbilical cord mesenchymal stem cells are a promising therapy for systemic lupus erythematosus based on cell biological roles. OBJECTIVE: To observe the therapeutic efficacy of human umbilical cord mesenchymal stem cell transplantation in the treatment of systemic lupus erythematosus in mice. METHODS: Human umbilical cord mesenchymal stem cells were isolated and cultured followed by labeling with DiR fluorescence. Experimental mice were divided into normal control group (C57BL mice), model control group (C57BL/lpr mice), low-, medium- and high-dose umbilical cord mesenchymal stem cell therapy groups (C57BL/lpr mice), with 10 mice in each group. Mice in the low-, medium- and high-dose groups were respectively injected 0.5×106, 1×106, 2×106 human umbilical cord mesenchymal stem cells, once a week, for 3 consecutive weeks. At the end of treatment, blood samples were collected to measure antinuclear antibody, anti-histone antibody, anti-double stranded DNA antibody changes; OPG and Foxp3 gene expression changes were detected by quantitative PCR method. RESULTS AND CONCLUSION: After treatment, the levels of anti-nuclear antibodies, anti-histone antibodies and anti-double stranded DNA antibodies in the peripheral blood of mice were all declined in the low-, medium- and high-dose groups, while the number of peripheral blood CD4+CD25+T cells was significantly elevated. OPG and Foxp3 gene expression was also increased dramatically in the low-, medium- and high-dose groups, which was similar to that in the normal control group and significantly different from that in the model control group (P < 0.01). Experimental findings demonstrate that after transplantation of human umbilical cord mesenchymal stem cells, all relevant indicators in C57BL/lpr mice recovered to the normal levels, and the high-dose treatment group had the most obvious effect. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source

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