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Sun T.,The Beijing Army General Hospital | Ye C.,The Beijing Army General Hospital | Zhang Z.,The Beijing Army General Hospital | Wu J.,The Beijing Army General Hospital | Huang H.,Beijing Hongtianji Neuroscience Academy
Cell Transplantation | Year: 2013

The present study investigated the ability of cotransplantation of Schwann cells (SCs) and olfactory ensheathing cells (OECs) combined with treadmill training in facilitating neuronal plasticity and promoting hindlimb function recovery of subacute moderate thoracic (T10) spinal cord contusion in rats. Two weeks postinjury, SCs were injected directly into the lesion, while OECs were injected into the adjacent tissues. The treadmill training with the rats began postinjury on day 7, with each session lasting 20 ± 10 min per day, 5 days per week, for 10 weeks. At the 11th week postinjury, OECs were found migrating longitudinally and laterally from the injection site to the injury site through the gray and white matter, while some traveled along the central canal or pia. The SCs remained densely packed and concentrated at the transplant site. The transplanted SCs supported ingrowth of numerous, densely populated neurofilament-positive (NF+), MBP+ axons. The OECs promoted elongation of moderate NF+, GAP-43+ axons and a few MBP+ axons in parallel with OEC processes. The GFAP immunoreactivity in the spared tissue surrounding the graft of SCs and OECs at the lesion site was less intense than that in the DMEM group. Treadmill training had no effect on GFAP immunoreactivity. Treadmill training increased the number of TH-immunoreactive neurons in the gray matter of L2 spinal cord. Moreover, cotransplantation of OECs and SCs significantly increased the BBB score during 5-8 weeks postinjury alongside treadmill training between 5 and 11 weeks. Cotransplantation of OECs and SCs combined with treadmill training resulted in the highest BBB score at 4 and 11 weeks. The study details the differential mechanisms of neuronal plasticity: (1) axon growth and remyelination induced by cotransplantation of OECs and SCs and (2) neuron plasticity below the lesion enhanced by treadmill training. The synergistic effects of the combined strategy enhance functional recovery. This manuscript is published as part of the International Association of Neurorestoratology (IANR) supplement issue of Cell Transplantation. © 2013 Cognizant Comm. Corp.


Zhao G.,the Beijing Army General Hospital | Liu X.,the Beijing Army General Hospital | Li F.,the Beijing Army General Hospital
Osteoporosis International | Year: 2016

Summary: The study investigated whether kyphoplasty (KP) was superior to vertebroplasty (VP) in treating patients with osteoporotic vertebral compression fractures (OVCFs). KP may be superior to VP for treating patients with OVCFs based on long-term VAS and ODI but not short-term VAS. Further large-scale trials are needed to verify these findings due to potential risk of selection bias. Introduction: This study aimed to assess whether KP was superior to VP in treating patients with OVCFs. Methods: The Medline, Embase, and Cochrane databases and references within articles and proceedings of major meetings were systematically searched. Eligible studies included patients with OVCFs who received either KP or VP. Standard mean differences (SMDs) and relative risks (RRs) were used as measures of efficacy and safety in a random-effects model. Results: Eleven studies enrolling 869 patients with OVCFs were identified as eligible for final analysis. Compared with VP, KP was associated with significant improvements in long-term (SMD, −0.70; 95 % confidence interval [CI]: −1.30, −0.10; P = 0.023) visual analog scale (VAS); short-term (SMD, −1.50; 95 % CI: −2.94, −0.07; P = 0.040) and long-term (SMD, −1.03; 95 % CI: −1.88, −0.18; P = 0.017) Oswestry Disability Indexes (ODIs); short-term (SMD, −0.74; 95 % CI: −1.42, −0.06; P = 0.032) and long-term (SMD, −0.71; 95 % CI: −1.19, −0.23; P = 0.004) kyphosis angles; and vertebral body height (SMD, 1.56; 95 % CI: 0.62, 2.49; P = 0.001) and anterior vertebral body height (SMD, 3.04; 95 % CI: 0.53, 5.56; P = 0.018). KP was also associated with a significantly longer operation time (SMD, 0.73; 95 % CI: 0.26, 1.19; P = 0.002) and a lower risk of cement extravasation (RR, 0.68; 95 % CI: 0.48, 0.96; P = 0.030) compared with VP. No significant differences were found in the short-term VAS, posterior vertebral body height, and adjacent-level fractures. Conclusion: Acknowledging some risk of selection bias, KP displayed a significantly better performance compared with VP only in one of the two primary endpoints, that is, for ODI but not for short-term VAS. Further randomized studies are required to confirm these results. © 2016, International Osteoporosis Foundation and National Osteoporosis Foundation.


Wu J.,The Beijing Army General Hospital | Sun T.,The Beijing Army General Hospital | Ye C.,The Beijing Army General Hospital | Yao J.,The Beijing Army General Hospital | And 2 more authors.
Cell Transplantation | Year: 2012

This study was designed to observe the functional changes after fetal olfactory ensheathing glia transplantation (OEGT) into the spinal cord of patients with chronic spinal cord injury (SCI). Patients whose recovery had plateaued for longer than 6 months were enrolled. Six thoracic patients were tested for safety and five cervical patients for efficacy. OEGT was performed according to the method developed by Huang. Average follow-up was 14 months (range 1.0-1.5 years). Sensation improved moderately (light touch 14.2, pin prick 13.6); as did spasticity (1-2 modified Ashworth scale down). Locomotion recovery was minimal (1.6). Useful reticular formation functions were observed, but due to a lack of appropriate outcome measure, they were not recorded and reported. © 2012 Cognizant Comm. Corp.


Ye C.,The Beijing Army General Hospital | Zhai L.,The Beijing Army General Hospital | Cai Y.,The Beijing Army General Hospital
Chinese Journal of Rehabilitation Medicine | Year: 2014

Objective: To explore the influence of olfactory ensheathing cell(OECs) transplantation combined with treadmill step training on neurotransmitter level and neurotrophic factor protein expression in spinal cord injury site in rats with spinal cord contusion.Method: Thirty- two adult female Sprague Dawley rats aged (75 ± 1)d were subjected to experimental spinal cord contusion at T10 level using a New York University impactor at the height of 25 mm. Rats were then randomly divided into 4 groups: group A, OECs transplantation combined with step training (OECs-step training) group; Group B, an OECs transplantation(OECs) group; Group C, step training combined with Dulbecco's modification of Eagle's medium(DMEM) injection (step training- DMEM) group; Group D,DMEM injection (SCI-DMEM) group. The OECs transplantation and DMEM injection were performed at 2 weeks post- injury. Step training began at the 7th d post- injury and consisted of daily sessions (once daily, 5d a week, for 10 weeks) of quadrupedal treadmill training, starting from 15 min and gradually increasing to 30 min daily, at speeds starting from 3m/min and gradually increasing in accordance to the condition of rats. At the 11th week postinjury, all the rats were executed and the spinal cord encompassing the injury site were removed. The levels of 5-hydroxytryptamine(5-HT), dopamine(DA), 3,4-dihydroxyphenyl alanine(DOPA) and norepinephrine (NE) of spinal cord lesion site were assessed by reversed-phase high performance liquid chromatographic methodologies(RP- HPLC), and the protein expressions of nerve growth factor(NGF), brain derived neurotrophic factor (BDNF) and neurotrophin-3(NT-3) in spinal cord lesion site were analyzed by Western blotting.Result: At the end of the 11th week post-injury, the DA and DOPA were not founded by RP-HPLC. An increased trend of 5-HT and DA levels in step training group, and expressions of BDNF, NGF and NT-3 protein in OECs group were observed. However, no synthetic effect on 5-HT, DA levels and BDNF, NGF, NT-3 protein expressions were demonstrated in OECs-step training group.Conclusion: OECs transplantation combined with step training fail to promote the secretion of 5-HT, DA and expressions of BDNF, NGF, NT-3 protein in spinal cord injury site.


Ye C.,The Beijing Army General Hospital | Sun T.,The Beijing Army General Hospital | Li F.,The Beijing Army General Hospital
Chinese Journal of Rehabilitation Medicine | Year: 2012

Objective: To explore quality of life(QOL) in patients with senile osteoporosis, cervical spondylosis, lumbar degenerative diseases, knee osteoarthritis and to identify the usefulness of Chinese version of short form-36 health survey (SF-36) in these patients above mentioned. Method: A total of 200 patients, respectively with senile osteoporosis, cervical spondylosis, lumbar degenerative diseases (each 50 cases) were recruited. SF-36 was used to evaluate QOL in these patients. Fifty community-dwelling elderly healthy person matched in age as control group were evaluated with SF-36. The reliability and validity were evaluated for the Cronbach's alpha coefficient, correlation coefficient respectively. Result: (1) Compared to the healthy elderly population, the total scores of SF-36 reduced in all patients except the patients with senile osteoporosis; the scores of physical functioning (PF), role limitation because of physical health (RP), general health perception (GH), vitality(VT) and social functioning(SF) decreased in patients with cervical spondylosis; while PF, RP, GH, hodily pain(BP) scores reduced in patients with lumbar degenerative diseases; PF RP, GH scores reduced in patients with knee osteoarthritis; only GH score reduced in patients with senile osteoporosis. (2)The total internal consistency of SF-36 was 0.726. Cronbach's alpha coefficient in PF, BP, GH, role limitation because of emotional health problem(RE) was above 0.7; in RP, VT, SF and mental health (MH) was above 0.6. All the correlation of coefficient of PF,BP,GH,RE,RP,VT,SF,MH were below Cronbach's alpha coefficient. Except the PF and RE, PF and MH, RE and MH, all the dimensions were correlated each other. Conclusion: QOL in patients with senile osteoporosis, cervical spondylosis, lumbar degenerative diseases, knee osteoarthritis were damaged in different severity, mainly in dimensions of PF, BP, GH, RP, VT. The SF-36 showed well reliability, validity, and usefulness for evaluation patients with senile osteoporosis, cervical spondylosis, lumbar degenerative diseases, knee osteoarthritis.


PubMed | the Beijing Army General Hospital
Type: Journal Article | Journal: Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA | Year: 2016

The study investigated whether kyphoplasty (KP) was superior to vertebroplasty (VP) in treating patients with osteoporotic vertebral compression fractures (OVCFs). KP may be superior to VP for treating patients with OVCFs based on long-term VAS and ODI but not short-term VAS. Further large-scale trials are needed to verify these findings due to potential risk of selection bias.This study aimed to assess whether KP was superior to VP in treating patients with OVCFs.The Medline, Embase, and Cochrane databases and references within articles and proceedings of major meetings were systematically searched. Eligible studies included patients with OVCFs who received either KP or VP. Standard mean differences (SMDs) and relative risks (RRs) were used as measures of efficacy and safety in a random-effects model.Eleven studies enrolling 869 patients with OVCFs were identified as eligible for final analysis. Compared with VP, KP was associated with significant improvements in long-term (SMD, -0.70; 95% confidence interval [CI]: -1.30, -0.10; P=0.023) visual analog scale (VAS); short-term (SMD, -1.50; 95% CI: -2.94, -0.07; P=0.040) and long-term (SMD, -1.03; 95% CI: -1.88, -0.18; P=0.017) Oswestry Disability Indexes (ODIs); short-term (SMD, -0.74; 95% CI: -1.42, -0.06; P=0.032) and long-term (SMD, -0.71; 95% CI: -1.19, -0.23; P=0.004) kyphosis angles; and vertebral body height (SMD, 1.56; 95% CI: 0.62, 2.49; P=0.001) and anterior vertebral body height (SMD, 3.04; 95% CI: 0.53, 5.56; P=0.018). KP was also associated with a significantly longer operation time (SMD, 0.73; 95% CI: 0.26, 1.19; P=0.002) and a lower risk of cement extravasation (RR, 0.68; 95% CI: 0.48, 0.96; P=0.030) compared with VP. No significant differences were found in the short-term VAS, posterior vertebral body height, and adjacent-level fractures.Acknowledging some risk of selection bias, KP displayed a significantly better performance compared with VP only in one of the two primary endpoints, that is, for ODI but not for short-term VAS. Further randomized studies are required to confirm these results.


PubMed | The Beijing Army General Hospital
Type: | Journal: Cell transplantation | Year: 2012

This study was designed to observe the functional changes after fetal olfactory ensheathing glia transplantation (OEGT) into the spinal cord of patients with chronic spinal cord injury (SCI). Patients whose recovery had plateaued for longer than 6 months were enrolled. Six thoracic patients were tested for safety and five cervical patients for efficacy. OEGT was performed according to the method developed by Huang. Average follow-up was 14 months (range 1.0-1.5 years). Sensation improved moderately (light touch 14.2, pin prick 13.6); as did spasticity (1-2 modified Ashworth scale down). Locomotion recovery was minimal (1.6). Useful reticular formation functions were observed, but due to a lack of appropriate outcome measure, they were not recorded and reported.

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