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Fedorova M.D.,Moscow State University | Andreeva I.P.,ZAO NVO Immunotek | Vilegzhanina E.S.,The All Russian State Center for Quality and Standardization of Veterinary Drugs and Feed | Komarov A.A.,The All Russian State Center for Quality and Standardization of Veterinary Drugs and Feed | And 3 more authors.
Applied Biochemistry and Microbiology | Year: 2010

A test-system based on enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of chloramphenicol (CAP) in foodstuff has been developed. The detection limit of the method was 0.05 μg/l. The procedures for milk samples preparation of various fat content and chicken muscles were optimized. Before the analysis milk was diluted 5-fold with a buffer. The detection limit for milk was 0.3 μg/l; recoveries varied from 74 to 118%. Two protocols for chicken muscles preparation were elaborated; extraction with buffer (the express method) and extraction with acetonitrile. The detection limits of CAP in chicken muscles were 0.5 and 0.3 μg/kg, respectively; recovery values were 71-107% and 95-115%, respectively. The results of residual amounts of CAP detection in foodstuff by ELISA and HPLC-MS were in good correlation. © 2010 Pleiades Publishing, Ltd. Source


Holma-Suutari A.,University of Oulu | Ruokojarvi P.,Finnish National Institute for Health and Welfare | Komarov A.A.,The All Russian State Center for Quality and Standardization of Veterinary Drugs and Feed | Makarov D.A.,The All Russian State Center for Quality and Standardization of Veterinary Drugs and Feed | And 8 more authors.
Environmental Sciences Europe | Year: 2016

Background: The Finnish and Russian animal species (semi-domesticated reindeer, Finnish wild moose, Baltic grey seal and Baltic herring) samples were biomonitored in terrestrial and aquatic environments for polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), polychlorinated biphenyls (PCBs) and polybrominated diphenylethers (PBDEs). Results: Grey seal (Halichoerus grypus) was clearly the most contaminated species. The mean PBDE concentration in grey seal was 115 ng/g fat, and the highest WHO-PCDD/F-PCB-TEQ (toxic equivalent set by WHO) was 327 pg/g fat. In Finnish, reindeer WHO-PCDD/F-TEQ varied from 0.92 pg/g fat in muscle to 90.8 pg/g fat in liver. WHO-PCDD/F-TEQ in moose liver samples was in the range of 0.7–4.26 pg/g fat, and WHO-PCB-TEQ in the range of 0.42–3.34 pg/g fat. Overall moose had clearly lower PCDD/F and DL-PCB concentrations in their liver than reindeer. Conclusions: Terrestrial animals generally had low POP concentrations, but in reindeer liver dioxin levels were quite high. All Finnish and Russian reindeer liver samples exceeded the EU maximum level [8] for PCDD/Fs (10 pg/g fat), which is currently set for bovine animals. © 2016, Holma-Suutari et al. Source


Yunin M.A.,The All Russian State Center for Quality and Standardization of Veterinary Drugs and Feed | Metalnikov P.S.,The All Russian State Center for Quality and Standardization of Veterinary Drugs and Feed | Komarov A.A.,The All Russian State Center for Quality and Standardization of Veterinary Drugs and Feed | Panin A.N.,The All Russian State Center for Quality and Standardization of Veterinary Drugs and Feed
Analytical and Bioanalytical Chemistry | Year: 2014

A rapid liquid chromatography tandem mass spectrometry method has been developed and validated for the determination of α-trenbolone, β-trenbolone, α-nortestosterone, β-nortestosterone, zeranol, and taleranol in bovine liver. The impact of liquid–liquid extraction with methyl tert-butyl ether and optimized solid phase extraction on silica cartridges significantly reduced effort and time of sample preparation. Electrospray ionization gives a significant signal increase compared with atmospheric pressure chemical ionization and atmospheric pressure photoionization. The HPLC gradient was optimized to separate isobaric analytes and matrix constituents from the hormone molecules. The optimized time and temperature of enzymatic hydrolysis of conjugated trenbolone was 4 h at 52 °C. The method validated in the range of 0.5–30 μg kg–1 for α-trenbolone, β-trenbolone, zeranol, taleranol, and 2–30 μg kg–1 for α-nortestosterone, β-nortestosterone. Combined uncertainty of measurements was in the range of 4 %–23 %. The matrix effect was negligible (1 %–5 %) for all analytes except of α-nortestosterone (19 %). The developed method with changes concerning sample size and hydrolysis was also applied for the analysis of meat, serum, and urine samples.[Figure not available: see fulltext.] © 2014 Springer-Verlag Berlin Heidelberg Source

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