Liu Q.-S.,Northern Sichuan Medical College |
Liu Q.-S.,Institute of Rheumatology and Immunology |
Yuan G.-H.,Institute of Rheumatology and Immunology |
Tang Z.,Northern Sichuan Medical College |
And 11 more authors.
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2012
Objective: To detect reticulated platelets (RPs) in patients with system lupus erythematosus (SLE) so as to explore the diagnostic and prognostic values of RPs for SLE. Methods: By using cytoanalyzer, platelets were analyzed in 80 SLE patients and 41 healthy subjects. According to the presence of thrombocytopenia, the SLE patients were divided into SLE plus thrombocytopenia group and non-thrombocytopenia group. Platelet-rich plasma was collected from all subjects through the intravenous blood. RPs were analyzed with flow cytometry. In addition, RPs were assayed in 11 SLE patients before and after medication. We counted the total number of megakaryocytes and classified them and studied the platelets in bone marrow of 21 SLE patients. Results: The initial RPs result was (6.73±6.02)% in SLE patients and (2.31±0.85)% in healthy subjects, with a significant difference (P<0.001). Among the 80 SLE patients, the RPs result was (10.99±7.54)% in the 15 patients, with thrombocytopenia, but (5.04±3.40)% in the 65 non-thrombocytopenia patients with a significant difference (P=0.001). The RPs result in 11 SLE patients significantly decreased from (8.59±7.01)% before treatment to (4.79±3.13)% after treatment (P=0.039). Bone marrow smear test showed that the number of platelets in the SLE patients with thrombocytopenia was significantly reduced compared with those non-thrombocytopenia ones. Thrombocytogenous megakaryocytes were significantly fewer in the patients with thrombocytopenia than in those without (P=0.007) although the two groups did not significantly differ in the number of immature megakaryocytes (P>0.05). Conclusion: RP has auxiliary diagnostic and prognostic values for SLE, especially in patients with thrombocytopenia. Thrombocytopenia in SLE patients may be related to the abnormal maturation of megakaryocytes in bone marrow.