The Affiliated Hospital of Jining Medical College

Jining, China

The Affiliated Hospital of Jining Medical College

Jining, China

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Chen Y.,Qingdao University | Chen Y.,the Affiliated Hospital of Jining Medical College | Xu W.,Qingdao University | Guo X.,the Affiliated Hospital of Jining Medical College | And 7 more authors.
European Radiology | Year: 2016

Purpose: To evaluate the utility of CT perfusion (CTP) for the assessment of superficial temporal artery-middle cerebral artery (STA-MCA) anastomosis in patients with Moyamoya syndrome (MMS). Subjects and methods: Twenty-four consecutive MMS patients, who underwent unilateral STA-MCA bypass surgery, received CTP before and after surgery. The relative perfusion parameter values of surgical hemispheres before treatment were compared with post-treatment values. All patients underwent CT angiography (CTA) before and after surgery in order to confirm the patency of bypass. Results: The follow-up CTA after surgery clearly demonstrated 20 (20/24, 83.3 %) bypass arteries, whereas four (16.7 %) bypass arteries were occluded or very small. Postoperative rMTT and rTTP values (P < 0.05) of the surgical side were significantly lower than pre-operation. In patients (n = 20) with bypass patency, postoperative rCBF, rMTT and rTTP values (P < 0.05) of the surgical side were significantly improved. However, the differences of all parameters were not significant (P > 0.05) in the patients (n = 4) without bypass patency after revascularization. Conclusion: This study demonstrates that CTP can provide a crucial quantitative assessment of cerebral haemodynamic changes in MMS before and after STA-MCA anastomosis. Key Points: • Twenty-four MMS patients undergoing STA-MCA bypass received CTP pre- and post-surgery • Cerebral haemodynamics improved on the surgical side post-surgery on CTP maps • rCBF might have a better correlation with patency of the bypass artery. • CTP can evaluate cerebral perfusion changes in MMS patients after cerebral revascularization © 2015, European Society of Radiology.


Lu J.,The First Peoples Hospital Of Jining | Sun P.,The Affiliated Hospital of Jining Medical College | Sun B.,The Second Peoples Hospital Of Jining | Wang C.,The First Peoples Hospital Of Jining
Medical Science Monitor | Year: 2015

Background: The present study aimed to compare the expression of liver kinase B1 (LKB1) in prostate cancer (PCa) tissues and the paired adjacent tissues, then to evaluate the statistical relationship between LKB1 expression and prognosis of PCa patients. Material/Methods: The relative expression of LKB1 at mRNA level was detected by quantitative real-time polymerase chain reaction (qRT-PCR). The expression of LKB1 at protein level was measured by immunohistochemistry (IHC) method. The relationship between LKB1 expression and clinicopathologic characteristics was estimated by chi-square test. Kaplan-Meier method was used to analyze the overall survival of PCa patients with different LKB1 expression. Cox regression analysis was performed to estimate the significance of LKB1 expression and clinicopathologic characteristics in the prognosis of PCa patients. Results: The relative expression of LKB1 at mRNA level was significantly lower in PCa tissues than in the normal tissues (P<0.001). The LKB1 expression was proved to be affected by clinical stage (P=0.019) and PSA concentration (P=0.031) of PCa patients. Moreover, patients with negative LKB1 expression had shorter survival than those with positive expression. Cox regression analysis confirmed that LKB1 could be regarded as a prognostic biomarker for PCa patients (P=0.001, HR=3.981, 95% CI=1.698–9.336). Conclusions: The expression of LKB1 was lower in PCa tissues and might be a predictor for the prognosis of PCa patients. © Med Sci Monit, 2015.


PubMed | Jining Stomatology Hospital, The Affiliated Hospital of Jining Medical College and Liaoning Medical University
Type: Journal Article | Journal: Bioscience reports | Year: 2016

Catalpol, an iridoid glucoside, has been reported to inhibit apoptosis of neuron and endothelial cells. In the present study, we investigated the mechanism of catalpol-mediated cardioprotection. The rat embryonic ventricular myocardial cell line (H9c2) cells were first incubated with catalpol, and then exposed to hydrogen peroxide (H2O2). The concentration of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) were all determined by using commercially available kits. Apoptotic cells were assessed by Hoechst 33258 and Annexin V-fluorescein isothiocyanate binding assay. Synthesis of Bcl-2, Bax, cytochrome c and caspase-3 were analysed by real-time semiquantitative reverse transcription-PCR and Western blotting. We observed that apoptosis in H9c2 was associated with increased Bax, cytochrome c, caspase-3, decreased Bcl-2 activity after 24h of H2O2 exposure. Catalpol pretreatment afforded a marked protection against the above H2O2-mediated cytotoxicity and apoptosis in H9c2 cells. Moreover, the catalpol pretreatment led to a great reduction in H2O2-induced MDA release and increased SOD. These findings indicated for the first time that pretreatment of H9c2 cells with catalpol can be against H2O2-induced apoptosis, and the protective effect of catalpol involves the mitochondrial-dependent caspase pathway and is associated with increased Bcl-2 and decreased Bax expression.


Zhou J.-W.,The Affiliated Hospital of Jining Medical College | Kong C.,The Affiliated Hospital of Jining Medical College | Luo J.,Zunyi Medical College | Cao J.,The Affiliated Hospital of Jining Medical College | Shi Y.,Tuberculosis Prevention and Control Institute of Jining City
Archives of Iranian Medicine | Year: 2013

Background: We compared the T cell antigen receptor (TCR-BV) gene families of peripheral blood mononuclear cells (PMBC) between children with tuberculosis (TB) and those inoculated with the Bacille Calmette Guerin (BCG) vaccine. Methods: The total RNA was extracted from PMBC of 15 TB children, 15 BCG-vaccinated children and 15 healthy controls. The RNAs were reverse-transcribed and amplified by polymerase chain reaction (PCR). PCR products were separated on 1.5% agarose gel and analyzed with the Genescan technique. Results: Some TCR-BV gene families in TB children and BCG-vaccinated children exhibited a blur band in the predicted position on 1.5% agarose gel, some showed a distinct or fainted band. In general, many shared predominant clonal TCR-BV gene families (Vβ2, Vβ16, Vβ21, Vβ22) and the restricted-expression families (Vβ14 and Vβ17). All the gene families of the control children only exhibited blur bands and polyclonal. Conclusions: The skewed profile of TCR-BV gene families in TB children and BCG-vaccinated children are similar, which may probably explain the protective effects of BCG-vaccine against TB in children.


Li X.,the Affiliated Hospital of Jining Medical College
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2012

To investigate the expression of breast cancer metastasis suppressor 1 (BRMS1) gene protein and the expression of BRMS1 gene promotor area methylation in supraglottic cancer and to evaluate its clinical significance. The expression of BRMS1 protein and BRMS1 gene promotor area methylation were examined by using Western blotting method and methylation-specific polymerase chain reaction(MSP) method in 70 cases of supraglottic cancer tissues and 60 cases of their surrounding laryngeal normal mucosa tissues (LNT) and 44 cases of cervical lymph node metastasis of supraglottic cancer. Western blot results indicate that BRMS1 protein expression is declined expression level in supraglottic cancer tissue than the expression of BRMS1 protein in LNT of supraglottic cancer. Compared with para carcinoma normal laryngeal mucous tissue, BRMS1 gene protein in supraglottic cancer tissue primary lesion decreased obviously, and it is decreased more obviously in cervical lymph node metastasis lesion, the discrepancy is notable (P < 0.05). MSP results indicate BRMS1 gene promotor methylation is coordinated with its down-expression in supraglottic cancer tissue. BRMS1 promotor area methylation analysis reveal that there were 34 patients with methylation in 70 patients' supraglottic cancer tumor primary lesion, hold 48.6% (34/70); 32 patients have methylation in 44 patients' cervical metastasis lymph node tissue, hold 72.7% (32/44); however, there is no methylation in 60 para carcinoma tissue (r(s) = 0.66, P < 0.05). The expression of BRMS1 protein in supraglottic cancer is significantly decreased. It had correlation with clinical stage and pathologic differentiation and cervical lymph node metastasis of supraglottic cancer. BRMS1 gene promotor methylation is related with down-expression of BRMS1 gene protein of supraglottic cancer. Maybe BRMS1 gene promotor methylation is one of the reasons of its down-expression.


Wang L.-F.,The Affiliated Hospital of Jining Medical College | Gu Z.-F.,The Affiliated Hospital of Jining Medical College | Wang B.-C.,Jinan Military General Hospital
Journal of Practical Oncology | Year: 2014

Objective: To investigate the effect of intratumorous injection of recombinant human endostain (Endostar) combined with cisplatin on the growth of Lewis lung cancer in mice.Methods: The Lewis lung cancer model was established in C57BL/6 mice. Tumor-bearing mice were randomly divided into 5 groups: group A (control group), group B (intraperitoneal injection of cisplatin), group C (intratumorous injection of Endostar), group D (intraperitoneal injection of cisplatin and Endostar), group E (intraperitoneal injection of cisplatin and intratumorous injection of Endostar). Tumor volume was measured on d11 and tumor inhibition rate (TIR) was calculated. The expression of VEGF and microvessel density (MVD) in tumor was detected by immunohistochemistry.Results: Compared to group A, TIRs of groups B, C, D and E were 61.8%, 24.9%, 70.3% and 74.3%, respectively. The expression of VEGF and MVD of group E were the lowest among all groups, significantly lower than that of group D(both P<0.05).Conclusion: Intratumorous injection of Endostar in combination with cisplatin exerts inhibitory effect on the growth of Lewis lung cancer in mice, and the effect was more marked than that of intraperitoneal injection. ©, 2014, Journal of Practical Oncology, Editorial Board. All right reserved.


Song Q.,the Affiliated Hospital of Jining Medical College
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2010

To investigate the possible mechanism of action of tripterygium glycosides (TG) for treatment of Behcet's disease (BD) through observing its effect on serum levels of interleukin-1beta (IL-1beta), interleukin-2 (IL-2), tumor necrosis factor alpha (TNF-alpha) and interferon-gamma (IFN-gamma). Thirty primarily treated BD patients (BD group) were treated with TG 30 mg/d orally for 3 months, and a control group consisting of 30 healthy persons was set up. Serum levels of IL-1beta, IL-2, TNF-alpha and IFN-gamma were detected by radio-immunosorbent assay (RIA) before and after treatment respectively. And the outcomes were analyzed in combining with the clinical status of patients as well as related indices as erythrocyte sedimentation (ESR) and C-reactive protein (CRP). Serum levels of IL-1beta, TNF-alpha, IFN-gamma in the BD group were evidently higher (P < 0.05) than those in the control group, they all lowered significantly after 3-month TG treatment, from 10.72 +/- 1.84 microg/L, 6.64 +/- 1.05 microg/L and 8.93 +/- 1.23 microg/L to 5.71 +/- 1.04 microg/L, 4.27 +/- 0.76 microg/L and 3.44 +/- 0.72 microg/L, respectively (P < 0.05), while level of IL-2 in the BD group was insignificantly different before treatment to that in the control (though showed an increasing trend) and was unchanged after treatment (P > 0.05). TG treatment showed the effectiveness of markedly effective in 10, effective in 16 and ineffective in 4 BD patients, with the total effective rate of 86.6%. Besides, ESR and CRP levels were evidently decreased in BD patients after treatment (P < 0.05). TG may treat BD by way of regulating the levels of IL-1beta,TNF-alpha and IFN-gamma.


Pang T.Z.,the Affiliated Hospital of Jining Medical College
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery | Year: 2012

To explore and evaluate the application of epiglottis with sternohyoid muscle in the surgery for laryngeal cancer. Two fifty patients with laryngeal cancer were treated by partial laryngectomy and the laryngeal defects were reconstructed by epiglottic flap and sternohyoid muscle fascia flap. The staging of tumors: T2N0M0 23 cases, T3N1M0 14 cases, T3N2M0 13 cases. All of them received post radical radiotherapy with average 60 Gy. The patients were followed up for 3 to 5 years. Thirty patients underwent neck dissection. The three and five years survival rates were 90% and 80% respectively. The total cases eat breath pronounced well. The decannulation rate was 96%. After partial laryngectomy in laryngeal cancer epiglottic flap and sternohyoid muscle fascia flap were performed reconstruction of laryngeal function.


Gan L.J.,The Affiliated Hospital of Jining Medical College
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2010

To evaluate the effect and safety of Xuesaitong (XST, a Panax Notoginseng extract preparation) via intracoronary injection for treating post-PCI slow-reflow phenomenon in patients with ST-segment elevation myocardial infarction (STEMI) and its impact on patients' prognosis. Thirty-nine STEMI patients who suffered from post-PCI slow-reflow after received percutaneous transluminal coronary angioplasty or stenting were assigned to two groups, 20 patients in the treated group and 19 in the control group. Intracoronary administering of 10 mL (0.5 mg) tirofiban and 400 mg XST were given to the treated group through guiding catheter, and followed with 36 h continuous intravenous dripping of tirofiban 10 mL/h and 400 mg XST in 250 mL of saline for dripping, while to the control group, the same intracoronary administering and intravenous dripping of tirofiban but without XST was given. The treatment was implemented for two days. Patients' coronary flow was assessed by the TIMI frame count method (TFC) at 1 min, 5 min and 10 min after injection; and the changes of ST-segment in 2 h, and incidence of bleeding in 48 h after medication were recorded. All patients were followed-up for 6 months to observe the incidence of cardiovascular events. Before the medication, the TIMI flow grade and the TFC in the treated group and the control group showed insignificantly statistical difference between groups (P > 0.05). After medication, 11 patients (55%) in the treated group and 8 patients (42%) in the control group with their blood flow reaching TIMI grade 3; the TFC decreased at 1, 5 and 10 min to 57.6 +/- 12.6, 46.1 +/- 9.3, 49.8 +/- 10.9 in the treated group and to 69.3 +/- 16.1, 61.2 +/- 15.3, 63.7 +/- 18.3 in the control group; and the 2 h ST segment fallback in them was 1.85 +/- 0.31 mm and 1.40 +/- 0.21 mm respectively, showing that the coronary blood flow in both groups were improved significantly after medication but the improvement in the former was better than in the latter group (P < 0.05). No case of death occurred in the hospitalization period. Results of 6-month follow-up study showed that the incidence of major adverse cardiac events, including angina pectoris, myocardial infarction, heart failure and cardiac death, was 33% in treated group and 44% in the control group, showing insignificant difference between groups (P > 0.05). Concomitant coronary injection with tirofiban and XST is more effective than that with tirofiban alone in improving the coronary blood flow and shows no increasing on the incidence of hemorrhagic complication.


PubMed | The Affiliated Hospital of Jining Medical College
Type: | Journal: Medical science monitor : international medical journal of experimental and clinical research | Year: 2016

BACKGROUND The aim of this study was to investigate the use of transcranial Doppler (TCD) for diagnosis of brain death in patients with severe cerebral injury. MATERIAL AND METHODS This retrospective study enrolled 42 patients based on inclusion and exclusion criteria. All patients were divided into either the brain death group or the survival group according to prognosis. Blood flow of the brain was examined by TCD and analyzed for spectrum changes. The average blood flow velocity (Vm), pulse index (PI), and diastolic blood flow in reverse (RDF) were recorded and compared. RESULTS The data demonstrated that the average speed of bilateral middle cerebral artery blood flow in the brain death group was significantly reduced (P<0.05). However, the PI of the brain death group increased significantly. Moreover, RDF spectrum and nail-like sharp peak spectrum of the brain death group was higher than in the survival group. CONCLUSIONS Due to its simplicity, high repeatability, and specificity, TCD combined with other methods is highly valuable for diagnosis of brain death in patients with severe brain injury.

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