The Affiliated Hospital of Hainan Medical College

Haikou, China

The Affiliated Hospital of Hainan Medical College

Haikou, China
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Li K.K.,Shanghai JiaoTong University | Li F.,Sun Yat Sen University | Li Q.S.,Shanghai JiaoTong University | Li Q.S.,South-Central University for Nationalities | And 3 more authors.
Anti-Cancer Agents in Medicinal Chemistry | Year: 2013

Epigenetic alterations have been implicated in the development and progression of human cancer. It is noteworthy that epigenetic modifications, in contrast to genetic mutations, are intrinsically reversible. This triggers an impressive interest of researchers in treatment of cancer patients via targeting epigenetic mechanisms, leading to subsequent intensive investigations of epigenetic drugs as a novel therapeutic intervention. DNA methylation, the major form of epigenetic modifications, is catalyzed by the maintenance DNA methyltransferase (DNMT) 1 and/or the de novo methyltransferases DNMT3A and DNMT3B. Aberrant expression of DNMTs and disruption of DNA methylation are closely associated with multiple forms of cancer, although the exact mechanisms underlying this link remain elusive. An array of tumor suppressor genes (TSGs) frequently sustain promoter hypermethylation, which results in epigenetic silencing of these genes and makes cancer cells acquire growth advantages. DNA demethylating agents, re-activating TSGs via inhibiting hypermethylation of their promoter regions, are currently being tested in clinical trials, and several of them are already applied in clinics. DNA demethylating agents, used either alone or in combination with other agents, such as chemotherapeutic drugs and the histone deacetylase inhibitors, have shown to be effective in treatment of cancer, although only in a small set of patients. In this review, we examine and discuss the most recent advances in epigenetic therapy of cancer, with a focus on DNA demethylating agents. © 2013 Bentham Science Publishers.


PubMed | Hainan Medical College, Hainan University, Yunnan University and The Affiliated Hospital of Hainan Medical College
Type: Journal Article | Journal: Molecular and cellular probes | Year: 2016

Sirtuin 6 (SIRT6) has recently been demonstrated to play an important role in the regulation of longevity in mammals. We therefore aimed to determine whether common variations in the SIRT6 gene are associated with human longevity. Five tag single nucleotide polymorphisms (SNPs) across the SIRT6 gene and its 5 kb up-/downstream region, including rs350852, rs350844, rs352493, rs4807546 and rs3760905, have been successfully determined in 616 unrelated Chinese long-lived individuals (LLIs) (mean age: 102.4 2.3 years) and 846 younger controls (mean age: 48.9 10.6 years) from Hainan Island, China. The allele and genotype frequencies of the five SNPs showed no statistically significant difference between the LLIs and controls (all P > 0.05). The five SNPs were in strong linkage disequilibrium and defined seven common haplotypes. Likewise, no association between these haplotypes and longevity was observed (all P > 0.05). The present study reveals that common genetic variations in the SIRT6 gene are not associated with human longevity.


PubMed | The Affiliated Hospital of Hainan Medical College, Monash University and University of Sichuan
Type: Journal Article | Journal: Expert review of anticancer therapy | Year: 2016

Nicotinamide phosphoribosyltransferase (Nampt) is the rate-limiting enzyme that catalyzes the first step in the mammalian nicotinamide adenine dinucleotide (NAD) salvage pathway. Aberrant NAD metabolism was associated with oncogenic signal transduction, suggesting the critical roles of Nampt in tumorigenesis and metastasis. Additionally, Nampt can be secreted out of the cell, and this extracellular form of Nampt (eNampt) was shown to induce inflammation and angiogenesis due to its cytokine activity, which may also be involved in carcinogenesis.This article reviews recent advances in the studies of Nampt in carcinogenesis, with a special highlight on Nampt inhibitors and future clinical application, including cancer diagnosis, prognosis and therapy. Expert commentary: Nampt not only maintains the balance of cellular metabolism, but also has a profound influence on multiple aspects of carcinogenesis. Therefore, elucidation of these mechanisms opens the door for future clinical applications targeting this protein. Additional studies are needed to address important questions including the relationship between extracellular Nampt and carcinogenesis.


Zheng C.,Southern Medical University | Zheng C.,The Affiliated Hospital of Hainan Medical College | Ma C.,The Affiliated Hospital of Hainan Medical College | Bai E.,The Affiliated Hospital of Hainan Medical College | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

A brain drug delivery system for glioma chemotherapy based on transferrin and cell-penetrating peptide dual-functioned liposome, Tf/TAT-lip, was made and evaluated with doxorubicin (DOX) as a model drug. TAT conjugated liposome (TAT-lip) loaded with doxorubicin (DOX) were prepared by the thin film hydration methods (lip-DOX) and then conjugated with transferrin (Tf) to yield Tf/TAT-lip-DOX which was characterized for their various physicochemical and pharmaceutical properties. Cellular uptakes were explored in both brain capillary endothelial cells (BCECs) of rats and U87 cells. The blood brain barrier model in vitro was established to evaluate the trans-endothelial ability crossing the BBB. The biodistribution of each formulation was further identified. The Tf/TAT-lip-DOX presents the best anti-proliferative activity against U87 cells. The orthotropic glioma model was established for the evaluation of anti-glioma effect. In conclusion, the experimental data in vitro and in vivo indicated that the Tf/TAT-lip was a promising brain drug delivery system due to its high delivery efficiency across the BBB. © 2015, E-Century Publishing Corporation. All rights reserved.


Jia C.-K.,The Affiliated Hospital of Hainan Medical College | Weng J.,The Affiliated Hospital of Hainan Medical College | Chen Y.-K.,The Affiliated Hospital of Hainan Medical College | Fu Y.,The Affiliated Hospital of Hainan Medical College
World Journal of Gastroenterology | Year: 2014

Aim: To report the devised anatomic liver resection of segments 6, 7 and 8 to improve the resection rate for patients with right liver tumors. Methods: We performed anatomic liver resection of segments 6, 7 and 8 to guarantee the maximum preservation of the remaining normal liver tissue. Segment 5 was determined by two steps of Glissonean pedicle occlusion. And a "⌈⌋" shaped broken resection line was marked upon the diaphragmatic surface of the liver. Selective right hemihepatic inflow occlusion was used to reduce blood loss during parenchymal transection between segments 6 and 5 and between segments 8 and 5. If needed, total hepatic Glissonean pedicle occlusion was used during parenchymal transection between segment 8 and the left liver. Results: Compared to right hemihepatectomy, the percentage of future liver remnant volume was increased by an average of 13.9% if resection of segments 6, 7 and 8 was performed. Resection of segments 6, 7 and 8 was completed uneventfully. After hepatectomy, the inflow and outflow of segment 5 were maintained. There was no perioperative mortality, postoperative abdominal bleeding or bile leakage in this group. Alpha-fetoprotein (AFP) returned to the normal range within 2 mo after the operation in all the patients. One patient died 383 d postoperatively due to obstructive suppurative cholangitis. One patient suffered from severe liver dysfunction shortly after surgery and had intrahepatic recurrence 4 mo postoperatively. Postoperative lung metastasis was found in one patient. No tumor recurrence was found in the other patients and the parameters including liver function and AFP level were in the normal range. Conclusion: Anatomic liver resection of segments 6, 7 and 8 can be a conventional operation to improve the overall resection rate for hepatocellular carcinoma. © 2014 Baishideng Publishing Group Co., Limited. All rights reserved.


Luo C.,University of Electronic Science and Technology of China | Li Q.,University of Sichuan | Li Q.,The Affiliated Hospital of Hainan Medical College | Xia Y.,University of Electronic Science and Technology of China | And 10 more authors.
Human Brain Mapping | Year: 2012

The basal ganglia, a brain structure related to motor control, is implicated in the modulation of epileptic discharges generalization in patients with idiopathic generalized epilepsy (IGE). Using group independent component analysis (ICA) on resting-state fMRI data, this study identified a resting state functional network that predominantly consisted of the basal ganglia in both healthy controls and patients with IGE. In order to gain a better understanding of the basal ganglia network(BGN) in IGE patients, we compared the BGN functional connectivity of controls with that of epilepsy patients, either with interictal epileptic discharges (with-discharge period, WDP) or without epileptic discharge (nondischarge period, NDP) while scanning. Compared with controls, functional connectivity of BGN in IGE patients demonstrated significantly more integration within BGN except cerebellum and supplementary motor area (SMA) during both periods. Compared with the NDP group, the increased functional connectivity was found in bilateral caudate nucleus and the putamen, and decreases were observed in the bilateral cerebellum and SMA in WDP group. In accord with the proposal that the basal ganglia modulates epileptic discharge activity, the results showed that the modulation enhanced the integration in BGN of patients, and modulation during WDP was stronger than that during NDP. Furthermore, reduction of functional connectivity in cerebellum and SMA, the abnormality might be further aggravated during WDP, was consistent with the behavioral manifestations with disturbed motor function in IGE. These resting-state fMRI findings in the current study provided evidence confirming the role of the BGN as an important modulator in IGE. © 2011 Wiley Periodicals, Inc.


Shuai H.-L.,Jinan University | Ye Q.,Pingxiang Peoples Hospital | Huang Y.-H.,The Affiliated Hospital of Hainan Medical College | Xie B.-G.,The Affiliated Hospital of Hainan Medical College
Andrologia | Year: 2015

Summary: The method of choice for assisted reproductive technology treatment in vitro fertilisation (IVF) or intracytoplasmic sperm injection (ICSI) is usually based on the evaluation of male infertility factors. Decisions for couples with moderate oligoasthenozoospermia (OA) are often empirical because uniform treatment criteria are lacking. This study aimed to evaluate the effect of patients with moderate OA treated with conventional IVF and ICSI. A total of 199 couples with moderate OA undergoing their first IVF/ICSI cycle were included in the study. The patients were divided into two groups according to the type of insemination: conventional IVF group (n = 97) and ICSI group (n = 102). All patients were randomised to be inseminated either by conventional IVF or ICSI. The fertilisation rate, embryo quality, implantation rate and clinical pregnancy rate were examined. No differences in the fertilisation, implantation and pregnancy rates were observed between conventional IVF and ICSI groups (P > 0.05). However, the number of good-quality embryos was significantly higher in the ICSI group than in the IVF group (P < 0.05). Couples with moderate OA did not influence on the overall clinical outcomes between IVF and ICSI treatments, and a negative influence by ICSI on blastocyst development was not confirmed. © 2014 Blackwell Verlag GmbH.


Luo C.,University of Electronic Science and Technology of China | Qiu C.,University of Electronic Science and Technology of China | Guo Z.,University of Electronic Science and Technology of China | Fang J.,University of Sichuan | And 9 more authors.
PLoS ONE | Year: 2012

Examining the spontaneous activity to understand the neural mechanism of brain disorder is a focus in recent resting-state fMRI. In the current study, to investigate the alteration of brain functional connectivity in partial epilepsy in a systematical way, two levels of analyses (functional connectivity analysis within resting state networks (RSNs) and functional network connectivity (FNC) analysis) were carried out on resting-state fMRI data acquired from the 30 participants including 14 healthy controls(HC) and 16 partial epilepsy patients. According to the etiology, all patients are subdivided into temporal lobe epilepsy group (TLE, included 7 patients) and mixed partial epilepsy group (MPE, 9 patients). Using group independent component analysis, eight RSNs were identified, and selected to evaluate functional connectivity and FNC between groups. Compared with the controls, decreased functional connectivity within all RSNs was found in both TLE and MPE. However, dissociating patterns were observed within the 8 RSNs between two patient groups, i.e, compared with TLE, we found decreased functional connectivity in 5 RSNs increased functional connectivity in 1 RSN, and no difference in the other 2 RSNs in MPE. Furthermore, the hierarchical disconnections of FNC was found in two patient groups, in which the intra-system connections were preserved for all three subsystems while the lost connections were confined to intersystem connections in patients with partial epilepsy. These findings may suggest that decreased resting state functional connectivity and disconnection of FNC are two remarkable characteristics of partial epilepsy. The selective impairment of FNC implicated that it is unsuitable to understand the partial epilepsy only from global or local perspective. We presumed that studying epilepsy in the multi-perspective based on RSNs may be a valuable means to assess the functional changes corresponding to specific RSN and may contribute to the understanding of the neuro-pathophysiological mechanism of epilepsy. © 2012 Luo et al.


PubMed | The Affiliated Hospital of Hainan Medical College
Type: Journal Article | Journal: Journal of medical ultrasonics (2001) | Year: 2016

The purpose of this study was to investigate whether focal fatty sparing arises from preexisting segmental homogeneous nonalcoholic fatty liver (NAFL).Patients with various patterns of fatty liver were recruited from among 16,127 people, and 132 participants with segmental homogeneous NAFL were followed during the second year. All participants underwent ultrasonography. The sonographic findings in segmental homogeneous NAFL were analyzed.Of the 16,127 participants, 1,619 had fatty liver (10.0%), and of these 1,568 had NAFL (9.72%) and 51 had alcoholic fatty liver (0.31%). Of the 1,568 patients with NAFL, 176 had homogeneous fatty liver with focal fatty sparing (11.2%), 132 had segmental homogeneous fatty liver (8.42%), and the rest had other patterns. At follow-up, of the 132 patients with segmental homogeneous fatty liver, 3 (2.3%) were found to have a normal liver and 26 (19.7%) diffuse homogeneous and heterogeneous fatty liver, and no focal fatty sparing was found (P<0.001).Focal fatty sparing usually does not arise in preexisting segmental homogeneous NAFL. This implies that newly appearing nodular hypoechogenicity in segmental homogeneous fatty liver is more likely to be a true lesion rather than focal fatty sparing.


PubMed | The Affiliated Hospital of Hainan Medical College
Type: Journal Article | Journal: Journal of medical ultrasonics (2001) | Year: 2016

To determine whether visualization of the elongated left lobe of the liver can be improved by distending the stomach with water and the implications of this technique.Forty-three out of 87 patients with elongated left lobe of the liver found at abdominal CT were included in this study after obtaining informed consent. Their livers were examined using ultrasound before and after drinking water. The left lobe of the liver was scanned from the subxiphoid and left subcostal approaches, with the sound beam directed to the spleen and the left lobe of the liver neighboring or behind the stomach; the elongated left lobe of the liver overlying the spleen was detected by scanning from the 9th-11th intercostal approach of the left flank.The overall visualization of the elongated left lobe of the liver before and after filling the stomach with water was 4.7% (2/43) and 100.0% (43/43), respectively (P<0.001); and the overall visualization of the spleen before and after filling the stomach with water was 4.7% (2/43) and 100.0% (43/43), respectively (P<0.001).Distending the stomach with sufficient water can substantially improve visualization of elongated left lobe of the liver at ultrasound, potentially improving detection of tumors in the liver and preventing misdiagnosis.

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