The Affiliated Hospital of Guiyang Medical College
The Affiliated Hospital of Guiyang Medical College
Zeng J.,the Affiliated Hospital of Guiyang Medical College |
He S.,the Affiliated Hospital of Guiyang Medical College |
Wu S.,the Affiliated Hospital of Guiyang Medical College
Chinese Journal of Interventional Imaging and Therapy | Year: 2016
Objective: To investigate the changes of nerves cross-sectional areas (CSA) in lower extremely, and the correlation between CSA changes and Toronto clinical scoring system (TCSS) score and disease duration in patients with type 2 diabetes. Methods: A total of 84 patients with type 2 diabetes (study group) and 30 age-matched healthy controls (control group) were enrolled in this study. The severity of diabetic sensorimotor polyneuropathy (DSP) was evaluated according to TCSS. The degrees included DSP absent (score of 0-5), mild (score of 6-8), moderate (score of 9-11), severe (12-19). Then patients with different severity of DSP were divided into various subgroups. According to disease duration, patients were divided into four different subgroups: 0-5 years, 6-10 years, 11-15 years and >15 years subgroup. Ultrasonography was performed on study group and control group, respectively. CSAs of saphenous, sural and posterior tibial nerves were recorded. And statistical analysis was done. Results: There were statistical differences among different subgroups of study group and control group (all P<0.05). Positive correlations were found between TCSS scores and nerve CSAs, including saphenous, sural and posterior tibial nerves (r=0.615, 0.632, 0.641, all P<0.05); as well was those between disease duration of diabetes and nerve CSAs (r=0.613, 0.643, 0.682, all P<0.05). Conclusion: Nerve CSAs changes by ultrasound relate to disease severity and duration in patients with type 2 diabetes. High-frequency ultrasound is a useful diagnostic tool for staging DSP especially. Copyright © 2016 by the Press of Chinese Journal of Medical Imaging and Technology.
Chen M.,University of Adelaide |
Chen M.,The Affiliated Hospital of Guiyang Medical College |
Norman R.J.,University of Adelaide |
Heilbronn L.K.,University of Adelaide
Current Diabetes Reviews | Year: 2011
Since the first in-vitro fertilisation (IVF) birth in 1978, the number of children born by assisted reproductive technologies (ART) continues to increase worldwide. However, the safety issues surrounding these procedures remain controversial, and the long term impact on human health is unknown. There is emerging evidence to indicate that IVF may predispose individuals to increased incidence of obesity, elevated blood pressure, fasting glucose and triglycerides and subclinical hypothyroidism. However, few studies have been conducted to date and the underlying mechanisms are unclear. This review will summarize the existing evidence in animal models and in humans, and will discuss epigenetic alterations, which may link manipulation of the pre-implantation embryo with increased risk of the later development of obesity, insulin resistance, type 2 diabetes and cardiovascular disease in offspring. Since these diseases are the leading cause of mortality and can be delayed or prevented by lifestyle modification, prospective follow up studies in IVF born adults are now urgently required to determine the degree of risks utilizing gold standard measures in human and animal models. © 2011 Bentham Science Publishers.
Luo J.,Chongqing Medical University |
Xu Y.,Chongqing Medical University |
Zhu Q.,Chongqing Medical University |
Zhao F.,Chongqing Medical University |
And 4 more authors.
Synapse | Year: 2011
Mical-1 is a novel F-actin-disassembly factor that is critical in actin reorganization. It provides a molecular conduit through which actin reorganizes-a hallmark of cell morphological changes, including axon navigation. However, whether Mical-1 is involved in the epileptogenesis remains unknown. Here, we investigate Mical-1 expression pattern in patients with intractable temporal lobe epilepsy (TLE) and pilocarpine-induced rat model. We used double-labeled immunoflurescence, immunohistochemistry, and Western blotting to assess the location and expression of Mical-1 in temporal neocortex of patients with intractable TLE, and the expression pattern of Mical-1 at different time point in the hippocampus and temporal lobe cortex of the pilocarpine-induced rat model. Double-labeled immunofluorescence showed that Mical-1 was coexpressed with neuron-specific enolase (NSE) in the cytoplasm of neurons in temporal neocortex of patients with TLE and hippocampus of rat model. Faint and scattered immunoreactivity for Mical-1 in the neuron of temporal neocortex in TLE group, but strong immunoreactivity for Mical-1 was shown in control subjects. To quantitatively evaluate the Mical-1 immunoreactivity, we measured the mean optical density (OD) of Mical-1. In the hippocampus of pilocarpine-induced rat model, the OD values transient increased at 6 h after seizure then decreased from 1 day to 14 days, and returned to a subnormal level at 60 days. The lowest level of Mical-1 expression occurred at 14 days after seizure in the hippocampus. In the temporal lobe cortex of rat model, the OD values decreased at all time point after kindling compared to the normal group. Furthermore, our Western blot analysis confirmed these expression patterns of Mical-1 from latent stage to chronic stage. Our results indicate that in patients with TLE and pilocarpine-induced rat model, the expression of Mical-1 were followed a downtrend from the latent stage to chronic stage after seizure evoke. Thus, as an effect factor participated in F-actin disassemble, Mical-1 may associate with inner pathophysiological modulation in epilepsy. © 2011 Wiley-Liss, Inc.
Xiang Y.-N.,Guiyang Medical College |
Xu L.-B.,The Affiliated Hospital of Guiyang Medical College
World Chinese Journal of Digestology | Year: 2015
AIM: To investigate the clinical, endoscopic and pathologic features, as well as differential diagnosis of gastrointestinal inflammatory fibroid polys (IFPs). Methods: The clinical data for 16 cases of IFPs were analyzed. The findings of endoscopy, endoscopic ultrasound, and histopathology were reviewed. Immunohistochemistry studies for Vimentin, CD34, CD117, discovered on GIST-1 (DOG-1), smooth muscle actin (SMA), S-100, and Desmin and cytokeratin (CK) were performed. Results: IFPs were mainly found in adults aged 40-60 years (56.25%), slightly more frequently in women. IFPs occurred mainly in the stomach (56.25%), small bowel (25%), and colon (18.75%), with the antrum being the most common place. The tumor might present as a polyp, submucosal mass or neoplasm-like mass. Endoscopic ultrasound showed hypoechoic lesions in the third or the second sonographic layer of the gastric wall but without involving the fourth layer, and the margin was poorly circumscribed. Histopathology showed that the tumor was mainly located in the submucosa and could invade the mucosa, but not the muscle layer. Microscopically, the main tumor components were fibroblast-like spindle cells, vascular and polymorphic inflammatory cells, usually dominant by eosinophils. The specific pathologic findings included eosinophils and concentric fibroblast-like spindle cells with an ‘onion-skin’-like appearance around vessels. Immunohistochemical staining of the spindle cells of IFPs showed 100% positivity for vimentin and 87.5% positivity for CD34. Treatments for IFPs included surgery and endoscopic submucosal dissection (ESD) or endoscopic mucosal resection (EMR). Conclusion: There are similar patterns for patient age and tumor location in IFPs. Endoscopy and endoscopic ultrasound can provide specific findings. The histopathological and immunohistochemical characteristics are useful for accurately diagnosing IFPs and differential diagnosis of the tumors occurring in the submucosa. © 2015 Baishideng Publishing Group Inc. All rights reserved.
Wang R.,University of Sichuan |
Wang R.,The Affiliated Hospital of Guiyang Medical College |
Lv Q.,University of Sichuan |
Meng W.,University of Sichuan |
And 4 more authors.
Journal of Thoracic Disease | Year: 2014
Background: Breast cancer stem cells (BCSCs) can be enriched by culturing of cells in non-adherent nondifferentiating conditions. However, culturing mammospheres from primary breast tumors are costly and difficult to control. In order to overcome problems associated with using primary human tissues, continuous breast cancer cell lines have been developed from various sources. Methods: In this study, a luminal subtype breast cancer cell line MCF-7 and a basal subtype cell line MDAMB- 231 were chosen. We explored the optimal culturing system for BCSCs from the two cell lines and primary breast tumors. Then, mammosphere formation efficiency (MFE), CD44+/CD24-/lowESA+Lin- cell proportion in mammospheres, and tumorigenecity of mammospheres generated from the two breast cancer cell lines and primary breast tumors were compared. Results: Enzymatic digestion of 60 mins and the addition of B27 to the culture medium were optimal for mammosphere culturing. Mammospheres could be formed in all the three cells, in which MCF-7 had the highest MFE. After 3 weeks culture, CD44+/CD24-/lowESA+Lin- cell proportion in mammospheres from MCF-7, MDA-MB-231 cells and primary breast tumors was 95.0%±2.5%, 82%±22% and 21.5%±1.0%, respectively. A total of 1,000 cells from MCF-7, MDA-MB-231 mammospheres but not primary mammospheres were tumorigenic. Conclusions: This study validates the use of breast cancer cell lines as models to elucidate the nature of BCSCs. © Pioneer Bioscience Publishing Company.
PubMed | University of Sichuan and the Affiliated Hospital of Guiyang Medical College
Type: Journal Article | Journal: Cancer biomarkers : section A of Disease markers | Year: 2016
Overexpression of extracellular matrix metalloproteinase inducer (EMMPRIN) or cluster of differentiation 147 (CD147), a glycoprotein enriched on the plasma membrane of tumor cells, promotes proliferation, invasion, metastasis, and survival of malignant tumor cells. In this study, we sought to examine the expression of EMMPRIN in breast tumors, and to identify the potential roles of EMMPRIN on breast cancer cells.EMMPRIN expression in breast cancer tissues was assessed by immunohistochemistry. We used a lentivirus vector-based RNA interference (RNAi) approach expressing short hairpin RNA (shRNA) to knockdown EMMPRIN gene in breast cancer cell lines MDA-MB-231 and MCF-7. In vitro, Cell proliferative, invasive potential were determined by Cell Counting Kit (CCK-8), cell cycle analysis and matrigel invasion assay, respectively. In vivo, tumorigenicity was monitored by inoculating tumor cells into breast fat pad of female nude mice.EMMPRIN was over-expressed in breast tumors and breast cancer cell lines. Down-regulation of EMMPRIN by lentivirus vector-based RNAi led to decreased cell proliferative, decreased matrigel invasion in vitro, and attenuated tumor formation in vivo.High expression of EMMPRIN plays a crucial role in breast cancer cell proliferation, matrigel invasion and tumor formation.
PubMed | University of Sichuan and the Affiliated Hospital of Guiyang Medical College
Type: Journal Article | Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae | Year: 2017
Objective To establish a Daphnia model of alloxan-induced diabetes. Methods Daphnia were exposed to three different concentrations of alloxan (3, 5, and 10 mmol/L) for 30 minutes. Blood glucose and survival rate were recorded for 72 hours after alloxan insult. Sequence analysis and phylogenetic inference for glucose transporters (GLUT) were clustered with the maximum-likelihood method. Using reverse transcription and quantitative polymerase chain reaction techniques, we investigated the transcriptional changes of GLUT at 12 hours after alloxan (5 mmol/L) exposure. Results Compared with control, 3 mmol/L, and 5 mmol/L as well as 10 mmol/L alloxan initially induced transient blood glucose decline by 15% for 2 hours and 12 hours respectively. In Daphnia with 5 and 10 mmol/L alloxan, their blood glucose was persistently raised by about 150% since after 24-hour insult. Survival rate of Daphnia exposure to alloxan with concentrations of 3, 5, and 10 mmol/L were 90%, 75%, and 25% respectively. We predicted seven GLUT genes in the Daphnia genome and successfully amplified them using real-time polymerase chain reaction. Two of seven GLUT transcripts were down-regulated in Daphnia with 5 mmol/L alloxan-induced diabetes. Conclusion Alloxan-induced diabetes model was successfully established in the Daphnia pulex, suggesting diabetes-relevant experiments can be conducted using Daphnia.
Liang X.,The Affiliated Hospital of Guiyang Medical College
Zhen ci yan jiu = Acupuncture research / [Zhongguo yi xue ke xue yuan Yi xue qing bao yan jiu suo bian ji] | Year: 2011
To study the mechanism of moxibustion in postponing the process of aging. Thirty Wistar rats were equally randomized into control, model and moxibustion groups. The subacute aging model was established by hypodermic injection of 25% D-galactose (125 mg/kg). Moxibustion was applied to bilateral "Shenshu" (GV 23) and "Pishu" (GV 20) once everyday for 6 weeks. After slicing, the pineal gland tissue was stained with HE and insitu hybridization methods respectively for detecting the quantity of pinealocytes and the expression of heat shock protein (HSP 70). Compared with the control group, both the quantity of pinealocytes and the expression of HSP 70 in the pineal gland in the model group were downregulated significantly (P < 0.001, P < 0.01). Compared with model group, the quantity of pinealocytes and of HSP 70 mRNA in the pineal gland of moxibustion group were upregulated significantly (P < 0.01, P < 0.05). Moxibustion can suppress aging induced decrease of pinealocyte number and HSP 70 expression in subacute aging rats, which may contribute to its effect in postponing aging.
Tang K.F.,The Affiliated Hospital of Guiyang Medical College
Zhonghua nan ke xue = National journal of andrology | Year: 2013
The pathogenesis of male infertility involves the interactions between environmental and genetic factors. An individual's susceptibility to male infertility is influenced by his internal abilities of metabolizing and detoxicating endogenous and exogenous chemicals. Glutathione-S-transferases (GSTs), such as enzymes, are involved in the cellular detoxication of various physiological and xenobiotic substances. Studies show that the polymorphism of the GSTs gene is correlated with male infertility. GSTs polymorphism-related susceptibilities to male infertility were found similar but with some inconsistencies within the same community, and inconsistent with some similarities among different communities. Therefore, further studies are to be done on the differences in GSTs polymorphism-related susceptibility to male infertility in different communities.
Guo H.,The Affiliated Hospital of Guiyang Medical College
Chinese Journal of Tissue Engineering Research | Year: 2012
BACKGROUND: At present, the intramedullary nailing fixation and plate fixation are the two main ways for the fixation of bone fracture. Inter-locking intramedullary nail with its wide indication, small trauma, stable fixation and other advantages has become the gold standard for the treatment of long bone fracture. OBJECTIVE: To compare the clinical effect of minimally invasive locking plate, expandable intramedullary nail and inter-locking intramedullary nail fixation for the treatment of humeral shaft fracture. METHODS: Sixty-six patients with humeral shaft fractures were divided into three groups according to different fixation methods. The three groups were treated with minimally invasive locking plate, expandable intramedullary nail and inter-locking intramedullary nail fixation respectively. The routine fix approach was applied in the three groups, and the healing time and the complications in the three groups were compared. RESULTS AND CONCLUSION: There was no significant difference of the healing time among three groups. There was no significant difference of complications among three groups after fixation (P > 0.05), but different in each group. Through this clinical observation, we can come to conclusion that the methods of three groups have good clinic curative effect in the treatment of humeral shaft fractures. In the clinical treatment, we should choice reasonable fixed way according to the kind of fracture and the extent of the damage.