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Wang Y.,The Affiliated Hospital of Binzhou Medical College
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2010

To investigate the role and clinical value of the serum level of hepatocyte growth factor (HGF) and vascular endothelial growth factor(VEGF) in laryngeal squamous cell cancer and the relationship between HGF, VEGF and clinicopathological factors. We measured serum HGF and VEGF level in 54 laryngeal squamous cell cancer patients using ELISA to demonstrate the variation of HGF, VEGF level before operation and at day 1, 3, 7, 14 post-surgery. The concentration of the serum HGF, VEGF content in 35 normal healthy people and in 30 vocal cords polyps patients were measured by the quantitative sandwich enzyme linked immunoassay technique. (1) The level of serum HGF and VEGF in the patients with laryngeal squamous cell cancer was significantly higher than that of normal healthy people and vocal cord polyps patients (P < 0.01). (2) The level of serum HGF, VEGF in the patients with laryngeal squamous cell cancer was related to clinical stage, differentiation situation, lymph nodes metastasis (P < 0.01), but it was independent of age at diagnosis, gender and tumor location (P > 0.05). (3) There was a significantly positive correlation between preoperative serum VEGF and HGF levels (r = 0.7667, P < 0.01). (4) Post-operational serum HGF levels in 54 laryngeal squamous cell cancer patients who underwent surgical intervention increased significantly, peaked at day 3 after operation. Serum HGF levels of survivors during follow-up period gradually decreased at day 7 and day 14 after operation. The postoperative serum levels of VEGF were decreased significantly than that of preoperation. HGF and VEGF may play an important role in the development and progression of human laryngeal cancer. Elevated serum HGF and VEGF levels predict a more aggressive biological behavior in laryngeal squamous cell cancer.


Zhang P.,The Affiliated Hospital of Binzhou Medical College | Zhou Z.,Qingdao Municipal Hospital
Cell Biochemistry and Biophysics | Year: 2015

Accumulated evidences support diabetic retinopathy is caused by functional and structural alterations in both the microvascular and neuroglial compartments. Here, we explored any beneficial effects of combination of Bevacizumab and NGF to prevent diabetic retinopathy. STZ-induced diabetic rats were randomly grouped into three groups: sham, Bevacizumab and combination group (Bevacizumab + NGF). End of the treatment, vascular abnormality was evaluated by FITC-dextran. Inflammation and angiogenesis factors were also investigated by real-time PCR and western blot. Our results showed both Bevacizumab and the combination could dramatically reduce abnormal vascular permeability and limit the inflammatory and angiogenesis in 6-week diabetic rats. However, the combination indicated better preventive effects in 12-week diabetic rats compared with Bevacizumab alone. Our findings suggest that the combination of Bevacizumab with NGF may be a new method for the preventive intervention of diabetic retinopathy. © 2015, Springer Science+Business Media New York.


Gao M.L.,The Affiliated Hospital of Binzhou Medical College | Wang A.G.,Shandong University
Genetics and Molecular Research | Year: 2015

The aim of this study is to explore the effect of narrow band ultraviolet B (NB-UVB) on the chemokine receptor CCR6 mRNA levels in patients with psoriasis. Psoriasis area and severity index (PASI) values were recorded before and after the treatment with NB-UVB phototherapy of 30 psoriasis vulgaris patients. The reverse transcription-polymerase chain reaction method was used to detect the expression level of CCR6 mRNA in peripheral blood mononuclear cells, and compared with 30 healthy subjects. The PASI value of the 30 psoriasis vulgaris patients decreased significantly after 15 iterations of phototherapy treatment (P < 0.01). The expression level of CCR6 mRNA in psoriasis patients was significantly higher than in the healthy controls (P < 0.01), while the expression level of CCR6 mRNA decreased significantly after phototherapy (P < 0.01). Reduction of CCR6 level may be one of the mechanisms through which NB-UVB can treat psoriasis. © FUNPEC-RP.


Zhou R.-J.,The Affiliated Hospital of Binzhou Medical College | Wang J.-Y.,The Affiliated Hospital of Binzhou Medical College
World Chinese Journal of Digestology | Year: 2014

AIM: To assess the value of insulin, C-peptide and blood glucose detection in the early diagnosis of diabetes with liver damage.RESULTS: Fasting blood glucose and 2-h postprandial blood glucose levels were significantly higher in the study group and control group than in the normal group (t = 2.317, 2.918, P < 0.05), and in the study group than in the control group (t = 3.084, 4.431, P < 0.05). Fasting insulin, 2-h postprandial fasting insulin, fasting C-peptide, 2-h postprandial C-peptide and insulin resistance index were significantly higher in the study group and control group than in the normal group (t = 2.276, 3.426, 5.317, 3.342, P < 0.05), and in the study group than in the control group (t = 6.436, 3.341, 2.195, 3.341, 3.426, 5.317, 3.342, P < 0.05). In diabetes patients with liver injury, blood glucose levels were positively correlated with insulin and C-peptide (r = 0.589, 0.724, P < 0.05). The diagnostic sensitivity and specificity of combined detection of the three indexes were 87.1% and 94.8%, significantly higher than those of detection of any single index.METHODS: Sixty-one diabetic patients with liver injury treated at our hospital were enrolled as a study group, 55 diabetic patients with normal liver function as a control group, and 50 healthy people as a normal group. The levels of blood glucose, insulin and C-peptide among the three groups were compared.CONCLUSION: Combined detection of blood glucose, insulin and C-peptide is helpful for early diagnosis of diabetes with liver injury. © 2014 Baishideng Publishing Group Inc. All rights reserved.


Zhang B.,Mie University | Xuan C.,Qingdao University | Ji Y.,Guangxi University | Zhang W.,The Affiliated Hospital of Binzhou Medical College | Wang D.,Guangxi University
Familial Cancer | Year: 2015

Zebrafish represents a powerful model for cancer research. Particularly, the xenotransplantation of human cancer cells into zebrafish has enormous potential for further evaluation of cancer progression and drug discovery. Various cancer models have been established in adults, juveniles and embryos of zebrafish. This xenotransplantation zebrafish model provides a unique opportunity to monitor cancer proliferation, tumor angiogenesis, metastasis, self-renewal of cancer stem cells, and drug response in real time in vivo. This review summarizes the use of zebrafish as a model for cancer xenotransplantation, and highlights its advantages and disadvantages. © 2015, Springer Science+Business Media Dordrecht.

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