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PubMed | The Affiliated Hospital of Binzhou Medical College, Binzhou Peoples Hospital, University of Oslo and Guangxi University
Type: | Journal: Clinica chimica acta; international journal of clinical chemistry | Year: 2017

The methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism is closely related to the acute lymphoblastic leukaemia (ALL) indicated by many previous epidemiologic studies. However, their conclusions were still conflicting.Our aim is to evaluate their associations using a more comprehensive updated meta-analysis. Electronic searches were conducted to select published studies prior to February, 2016.Totally, 39 case-control studies including 6551 ALL cases and 10,918 controls were selected in current meta-analysis. The association was detected significantly between MTHFR C677T polymorphism and ALL reducing susceptibility.Our results indicate that the MTHFR C677T polymorphism may be a promising ALL biomarker and studies to explore the protein levels of the variants and their functional role are required for the definitive conclusions.


PubMed | The Affiliated Hospital of Binzhou Medical College, Shandong University of Traditional Chinese Medicine and Shandong University
Type: Journal Article | Journal: Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association | Year: 2016

To review the results of the treatment of post-traumatic chronic osteomyelitis of lower limbs by bone transport technique using mono-lateral external fixator.A total of 18 patients with post-traumatic chronic osteomyelitis of lower limbs were treated by bone transport technique using mono-lateral external fixator in this study. Seven cases (patients No. 1-7) were performed early BGPO (autologous ilium bone grafting and plate osteosynthesis) intervention after BT (bone transport). The end results were assessed by the criteria of ASAMI and Paley etal. Some data between the cases with and without the intervention of early BGPO were subjected to statistical analysis of Students t-test.The overall average time for union was 10.8 months. The average union time of the non-early BGPO cases was 52.277.82 weeks while the early BGPO cases took a shorter time of 36.865.34 weeks (P<0.001). The overall average duration from bone transport to removal of external fixator was 10.0 months. The average duration from bone transport to removal of external fixator in the non-early BGPO cases was 51.557.45 weeks while the early BGPO cases took a shorter time of 29.295.47 weeks (P<0.001). The overall average of BHI was 1.77monthscm(-1). The average of BHI in the non-early BGPO cases was 2.250.25monthscm(-1) and the average BHI of the early BGPO cases was 1.220.114monthscm(-1) (P<0.001). There were 31 additional surgeries and 38 complications in total after bone transport treatment. The bone results were excellent in 15 cases, good in 3 cases. The functional results were excellent in 9 cases, good in 6 cases and fair in 3 cases.1. Bone transport technique using mono-lateral external fixator is an effective method for the treatment of post-traumatic chronic osteomyelitis. 2. Early BGPO intervention after bone transport is a feasible method to shorten the external fixation time and the total duration of treatment.


Zhang P.,The Affiliated Hospital of Binzhou Medical College | Zhou Z.,Qingdao Municipal Hospital
Cell Biochemistry and Biophysics | Year: 2015

Accumulated evidences support diabetic retinopathy is caused by functional and structural alterations in both the microvascular and neuroglial compartments. Here, we explored any beneficial effects of combination of Bevacizumab and NGF to prevent diabetic retinopathy. STZ-induced diabetic rats were randomly grouped into three groups: sham, Bevacizumab and combination group (Bevacizumab + NGF). End of the treatment, vascular abnormality was evaluated by FITC-dextran. Inflammation and angiogenesis factors were also investigated by real-time PCR and western blot. Our results showed both Bevacizumab and the combination could dramatically reduce abnormal vascular permeability and limit the inflammatory and angiogenesis in 6-week diabetic rats. However, the combination indicated better preventive effects in 12-week diabetic rats compared with Bevacizumab alone. Our findings suggest that the combination of Bevacizumab with NGF may be a new method for the preventive intervention of diabetic retinopathy. © 2015, Springer Science+Business Media New York.


PubMed | The Affiliated Hospital of Binzhou Medical College and Qingdao Municipal Hospital
Type: Journal Article | Journal: Cell biochemistry and biophysics | Year: 2016

Accumulated evidences support diabetic retinopathy is caused by functional and structural alterations in both the microvascular and neuroglial compartments. Here, we explored any beneficial effects of combination of Bevacizumab and NGF to prevent diabetic retinopathy. STZ-induced diabetic rats were randomly grouped into three groups: sham, Bevacizumab and combination group (Bevacizumab+NGF). End of the treatment, vascular abnormality was evaluated by FITC-dextran. Inflammation and angiogenesis factors were also investigated by real-time PCR and western blot. Our results showed both Bevacizumab and the combination could dramatically reduce abnormal vascular permeability and limit the inflammatory and angiogenesis in 6-week diabetic rats. However, the combination indicated better preventive effects in 12-week diabetic rats compared with Bevacizumab alone. Our findings suggest that the combination of Bevacizumab with NGF may be a new method for the preventive intervention of diabetic retinopathy.


Zhang B.,Mie University | Xuan C.,Qingdao University | Ji Y.,Guangxi University | Zhang W.,The Affiliated Hospital of Binzhou Medical College | Wang D.,Guangxi University
Familial Cancer | Year: 2015

Zebrafish represents a powerful model for cancer research. Particularly, the xenotransplantation of human cancer cells into zebrafish has enormous potential for further evaluation of cancer progression and drug discovery. Various cancer models have been established in adults, juveniles and embryos of zebrafish. This xenotransplantation zebrafish model provides a unique opportunity to monitor cancer proliferation, tumor angiogenesis, metastasis, self-renewal of cancer stem cells, and drug response in real time in vivo. This review summarizes the use of zebrafish as a model for cancer xenotransplantation, and highlights its advantages and disadvantages. © 2015, Springer Science+Business Media Dordrecht.


Wang Y.,the Affiliated Hospital of Binzhou Medical College
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2010

To investigate the role and clinical value of the serum level of hepatocyte growth factor (HGF) and vascular endothelial growth factor(VEGF) in laryngeal squamous cell cancer and the relationship between HGF, VEGF and clinicopathological factors. We measured serum HGF and VEGF level in 54 laryngeal squamous cell cancer patients using ELISA to demonstrate the variation of HGF, VEGF level before operation and at day 1, 3, 7, 14 post-surgery. The concentration of the serum HGF, VEGF content in 35 normal healthy people and in 30 vocal cords polyps patients were measured by the quantitative sandwich enzyme linked immunoassay technique. (1) The level of serum HGF and VEGF in the patients with laryngeal squamous cell cancer was significantly higher than that of normal healthy people and vocal cord polyps patients (P < 0.01). (2) The level of serum HGF, VEGF in the patients with laryngeal squamous cell cancer was related to clinical stage, differentiation situation, lymph nodes metastasis (P < 0.01), but it was independent of age at diagnosis, gender and tumor location (P > 0.05). (3) There was a significantly positive correlation between preoperative serum VEGF and HGF levels (r = 0.7667, P < 0.01). (4) Post-operational serum HGF levels in 54 laryngeal squamous cell cancer patients who underwent surgical intervention increased significantly, peaked at day 3 after operation. Serum HGF levels of survivors during follow-up period gradually decreased at day 7 and day 14 after operation. The postoperative serum levels of VEGF were decreased significantly than that of preoperation. HGF and VEGF may play an important role in the development and progression of human laryngeal cancer. Elevated serum HGF and VEGF levels predict a more aggressive biological behavior in laryngeal squamous cell cancer.


Gao M.L.,The Affiliated Hospital of Binzhou Medical College | Wang A.G.,Shandong University
Genetics and Molecular Research | Year: 2015

The aim of this study is to explore the effect of narrow band ultraviolet B (NB-UVB) on the chemokine receptor CCR6 mRNA levels in patients with psoriasis. Psoriasis area and severity index (PASI) values were recorded before and after the treatment with NB-UVB phototherapy of 30 psoriasis vulgaris patients. The reverse transcription-polymerase chain reaction method was used to detect the expression level of CCR6 mRNA in peripheral blood mononuclear cells, and compared with 30 healthy subjects. The PASI value of the 30 psoriasis vulgaris patients decreased significantly after 15 iterations of phototherapy treatment (P < 0.01). The expression level of CCR6 mRNA in psoriasis patients was significantly higher than in the healthy controls (P < 0.01), while the expression level of CCR6 mRNA decreased significantly after phototherapy (P < 0.01). Reduction of CCR6 level may be one of the mechanisms through which NB-UVB can treat psoriasis. © FUNPEC-RP.


PubMed | The Affiliated Hospital of Binzhou Medical College
Type: | Journal: Medical science monitor : international medical journal of experimental and clinical research | Year: 2016

Dysregulation of miR-9 is a common feature of many types of cancers, including oral squamous cell carcinoma (OSCC). However, whether the expression level of serum miR-9 is changed in patients with OSCC remains unknown.Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to examine the expression level of serum miR-9 in OSCC patients, oral leukoplakia (OLK) patients, and healthy volunteers, then we evaluated the association between serum miR-9 expression level and clinical outcome of OSCC patients.The expression level of serum miR-9 was significantly downregulated in patients with OSCC or OLK in comparison with healthy controls (P<0.01). Serum miR-9 expression level was associated with various clinicopathological parameters, including T stage (P=0.013), lymph node metastasis (P=0.002), and TNM stage (P=0.007). In addition, the OSCC patients in the low serum miR-9 expression group had poorer overall survival rate (P=0.022) and disease-free survival rate (P=0.004) compared with those in the high serum miR-9 expression group. Multivariate analysis showed that serum miR-9 was an independent prognostic factor for OSCC.Serum miR-9 was downregulated in patients with OSCC and patients with OLK. In addition, low serum miR-9 was correlated with poor prognosis of OSCC, indicating miR-9 might play a tumor suppressive role in OSCC and can serve as a promising biomarker for this deadly disease.


PubMed | The Affiliated Hospital of Binzhou Medical College
Type: Journal Article | Journal: Biological & pharmaceutical bulletin | Year: 2016

Psoralen is a major active component of Psoralea corylifolia. In the present study, we analyzed psoralen-induced changes in human breast cancer MCF-7/ADR cells and investigated the underlying mechanisms of the anticancer effect on MCF-7/ADR cells. We measured cell viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to evaluate the cytotoxicity and multidrug resistance (MDR) reversal activity of psoralen. The cell cycle distribution and apoptosis, accumulation and efflux of rhodamine123 (Rh123), and P-glycoprotein (P-gp) expression levels of MCF-7/ADR cells treated with psoralen were all detected by flow cytometry (FCM). We assessed P-gp ATPase activity by monitoring ATP consumption. We evaluated the activity of nuclear factor-kappaB (NF-B) and the expression of E-cadherin, vimentin and -smooth muscle actin (SMA) involved in regulating epithelial-mesenchymal transition (EMT). The results showed that psoralen inhibited the proliferation of MCF-7/ADR cells as shown by G0/G1 phase arrest rather than encouraging apoptosis. It was also observed that psoralen reversed MDR through inhibiting ATPase activity rather than reducing P-gp expression. Our results further showed that psoralen inhibited the migration abilities of MCF-7/ADR cells by repressing EMT possibly through inhibiting the activation of NF-B. Our findings provided a systematic and detailed description of the anti-cancer effect of psoralen on MCF-7/ADR cells for the exploration of natural compounds as novel anticancer agents.


PubMed | The Affiliated Hospital of Binzhou Medical College and Shandong University
Type: | Journal: Cytokine | Year: 2016

Interleukin-35 (IL-35) is a recently discovered inhibitory cytokine, which is firstly discovered to be produced by regulatory T cells (Tregs) and proposed as a key effector molecule of Treg function. This study aims to analyze the correlation between IL-35 expression in tumor-infiltrating lymphocytes (TILs) of breast cancer tissue and patients clinical characteristics. Plasma IL-35 was also determined in 60 patients with breast invasive ductal carcinoma (IDC) and 30 healthy women by enzyme-linked immunosorbent assay. IL-35 expression in the tissue specimens was analyzed by immunohistochemistry. It was shown that 39.1%, 43.6% and 17.3% of the 110 patients were absent, weak, and strong IL-35 expression in the TILs, respectively. Strong IL-35 expression in TILs was significantly associated with age >50years, tumor size >2cm, TNM stage III, and negative ER (All P<0.05). Patients with elevated IL-35 expression in TILs had significantly worse progression-free survival (PFS) and overall survival (OS) than patients with weak or no IL-35 expression (All P<0.05). High plasma IL-35 levels were significantly associated with TNM stage III and lymph node metastasis (All P<0.05). Plasma IL-35 level and IL-35 expression in the TILs of breast cancer tissues may be a valuable biomarker in the development and prognosis of IDC.

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