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Dang J.,PLA Fourth Military Medical University | Dang J.,Affiliated Hospital of the Academy of Military Medical science | Bian Y..-Q.,PLA Fourth Military Medical University | Sun J.Y.,PLA Fourth Military Medical University | And 7 more authors.
Journal of Oral Pathology and Medicine | Year: 2013

Oral lichen planus (OLP) is a common oral mucosal disease, which is generally considered a potentially malignant lesion. To identify efficiently prognostic biomarker, we investigated the microRNA-137 (miR-137) promoter methylation in OLP and compared with the samples from healthy volunteers and patients with oral squamous cell carcinoma (OSCC). A total of 20 OLP and 12 patients with OSCC as well as 10 healthy subjects were subjected to miR-137 promoter methylation analysis using methylation-specific PCR (MSP). To address the malignancy prediction potential from miR-137 promoter methylation status, methylation of the p16 gene, a well-known tumor suppressor, was investigated in the same samples. The p16 methylation and miR-137 promoter methylation were found to be 25% and 35% in patients with OLP, 50% and 58.3% in patients with OSCC, and 0% and 0% in healthy subjects, respectively. The differences between miR-137 and p16 methylation levels were statistically significant between healthy controls and patients. Methylation levels of the two promoters were also influenced by age, gender, and lesion duration. Interestingly, aberrant promoter methylation of the p16 and miR-137 genes was only found in the epithelium but not in the connective tissue from patients with OLP. This raises the possibility to use miR-137 methylation as a biomarker for malignant prediction in patients with OLP. © 2012 John Wiley & Sons A/S.

Zhou C.,Beihang University | Zhou C.,Peking University | Zhou J.,Beihang University | Zhou J.,Peking University | And 5 more authors.
Acta Biochimica et Biophysica Sinica | Year: 2012

Zinc protoporphyrin IX (ZnPPIX), a heme oxygenase-1 enzyme inhibitor, has been reported to induce apoptosis and to have antitumor properties. Here, we report that ZnPPIX triggers autophagy and causes defective autophagy flux in HeLa cells. Autophagosome formation was independent of Beclin 1, indicating non-canonical autophagy activity in ZnPPIX-treated cells. Furthermore, western blot results indicated that p38 MAPK (mitogen-activated protein kinase) was phosphorylated in treated cells. Consistently, SB203580 (a p38 inhibitor) obviously inhibited the accumulation of autophagosomes. Our results indicated that p38 MAPK may be a key regulator for non-canonical Beclin1-independent autophagy. © 2012 The Author.

Kang C.-Y.,Chinese PLA General Hospital | Zou L.,Chinese PLA General Hospital | Yuan M.,China Astronaut Research and Training Center | Wang Y.,Chinese PLA General Hospital | And 6 more authors.
European Journal of Applied Physiology | Year: 2011

Cardiovascular deconditioning is known to occur in astronauts exposed to microgravity. Endothelial dysfunction at microcirculatory sites might contribute to cardiovascular deconditioning induced by weightlessness. Recent studies have reported changes in the morphology and gene expression of endothelial cells exposed to conditions of simulated microgravity. The present study was aimed at examining the effects of microgravity on the apoptosis of microvascular endothelial cells and the mechanism underlying these effects. We simulated a microgravity environment and found that microgravity induced microvascular endothelial cell apoptosis and that this effect was correlated with the downregulation of the PI3K/Akt pathway, increased expression of NF-κB, and depolymerization of F-actin. These findings may provide important insights into the origin of the adverse physiological changes occurring due to exposure to microgravity conditions. © Springer-Verlag 2011.

Liu X.L.,Affiliated Hospital of the Academy of Military Medical science
Zhonghua nei ke za zhi [Chinese journal of internal medicine] | Year: 2011

To summarize the clinical features of mercury poisoning diagnosed by blood and urine tests for improving the diagnosis and treatment of the disease. Poisoning causes, clinical manifestations, diagnosis, treatment and prognosis were retrospectively reviewed in 92 in-patients with mercury poisoning in our hospital from January 2000 to April 2010. Of the 92 patients, 37 were male and 55 were female with an average age of 33.1 (2 - 65) years old. The mercury poisoning was caused by occupational exposure and non-occupational exposure, such as iatrogenic exposure, life exposure and wrong intake or suicidal intake of mercury-containing substances, mainly through respiratory tract, digestive tract and skin absorption. The most common clinical symptoms were as the followings: nervous system symptom, such as memory loss in 50 cases (54.3%), fatigue in 34 (37.0%), numb limb in 25 (27.2%), dizziness and headache in 22 (23.9%), cacesthesia in 20 (21.7%), fine tremor (finger tip, tongue tip, eyelids) in 15 (16.3%), insomnia and more dreams in 12 (13.0%); gastrointestinal symptoms: nausea in 16 (17.4%), abdominal pain in 14 (15.2%), stomatitis in 5 (5.4%); joint and muscle symptoms: muscle pain in 16 (17.4%), joint pain in 5 (5.4%); cardiovascular system: chest tightness, heart palpitations in 6 (6.5%); urinary system: edema in 9 (9.8%); other system: hidrosis in 20 (21.7%). After the treatment with sodium dimercaptopropane sulfonate (DMPS), the symptoms were gradually alleviated. Their gastrointestinal, cardiovascular symptoms were alleviated within 2 weeks; neurological symptoms were alleviated within 3 months; kidney damage showed a slower recovery and could be completely alleviated within 6 months. Because of its diverse clinical symptoms, the mercury poisoning was easy to misdiagnosis and missed diagnosis; therefore the awareness of the disease should be further enhanced. Leaving from the poisoning environment timely and giving appropriate treatment with DMPS will lead to a satisfactory prognosis.

Zeng J.,Chinese PLA General Hospital | Zeng J.,Affiliated Hospital of the Academy of Military Medical science | Fang Y.,Affiliated Hospital of the Academy of Military Medical science | Li P.,Chinese Peoples Liberation Army
Tumor Biology | Year: 2014

FAS is a cell surface receptor involved in apoptotic signaling in many cell types including cells of the immune system. The -1377 A/G polymorphism of FAS gene has been detected in breast cancer. However, the published evidence regarding the -1377 A/G polymorphism and breast cancer risk has generated controversial results. We performed a meta-analysis of five case-control association studies totaling to 5,995 study subjects including 2,905 cases and 3,090 controls. The combined odd ratios (ORs) with its 95 % CIs were used to assess the association of the -1377 A/G polymorphism correlated with breast cancer susceptibility with the fixed-effects model. The combined results showed significantly increased risk associated with the -1377 A/G polymorphism under AA vs. GG genetic model (OR = 1.28, 95 % CI = 1.04-1.58; heterogeneity test: P = 0.614, I 2 = 0.0 %), AA vs. GA + GG genetic model (OR = 1.24, 95 CI = 1.02-1.51; heterogeneity test: P = 0.349, I 2 = 10.0 %), and allele model A vs. G (OR = 1.10, 95%CI = 1.02-1.20; heterogeneity test: P = 0.422, I 2 = 0.0 %). Similarly, significant association was found in Asians. In stratified analyses by control source, a higher risk was indicated in the hospital-based studies rather than the population-based studies. This meta-analysis suggests that the -1377 A/G polymorphism is likely to be associated with the risk of breast cancer, especially the A allele in Asians. © 2013 International Society of Oncology and BioMarkers (ISOBM).

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