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Shi Y.,The 97th Hospital of PLA | Du B.-T.,Jiangsu AOKE Biomedical Technology Co. | He Y.-Q.,Jiangsu University | Yin W.-J.,Jiangsu University
Chinese Journal of Tissue Engineering Research | Year: 2014

Background: A cross-emulsification patented product of microporous polysaccharide hemostatic powder was prepared using potatoes as raw material with independent intellectual property rights. Objective: To observe hemostasis effects of microporous polysaccharide hemostatic powder on soft tissue trauma. Methods: A wound, about 3 cm long and 0.5 cm deep, was made on the abdominal soft tissue of rabbits in the experimental group 1, and then microporous polysaccharide hemostatic powder, 1.0-2.0 g, was sprayed directly on the wound. In the experimental group 2, a wound, about 3.0 cm long and 1.0 cm deep, was made on the abdominal soft tissue of rabbits, and then microporous polysaccharide hemostatic powder, 1.0-2.0 g, was sprayed directly on the wound. Another rabbits with untreated wound served as controls. Results And Conclusion: Microporous polysaccharide hemostatic powder formed a "pasty gel" covering the bleeding wound that achieved hemostatic effect. The bleeding time was (15.25±1.04) seconds in the experimental group 1 and (11.25±1.89) seconds in the experimental group 2. The marked effective and effective rates for hemostasis were 87.5% and 100%, respectively. In the control group, the bleeding time was more than 5 minutes. Hematoxylin-eosin staining showed mild muscle edema, vasodilatation of small blood vessels, and few scattered endoplasmic neutrophils infiltrated at 24 hours after treatment with microporous polysaccharide hemostatic powder; till the 7th day, inflammation subsided, mild fibrosis was visible on muscle tissue surface, the hemostatic powder was completely absorbed, the wound tissue was close to the normal tissue, and there were no significant changes in muscle cells. These findings suggest that microporous polysaccharide hemostatic powder can be used for hemostasis of soft tissue trauma. Source


Liu Z.,Institute of Tuberculosis Research | Zhou G.,The 309th Hospital of PLA | Deng X.,The 97th Hospital of PLA | Yu Q.,The 309th Hospital of PLA | And 4 more authors.
Journal of Infection | Year: 2014

Objectives: The regulatory mechanism of microRNA (miRNA) within macrophage innative response to Mycobacterium tuberculosis infection is not clear yet. Methods: The expression profile of cellular miRNAs during Mycobacterium bovis BCG infection was analyzed by using microarray. The expression of miR-146a was evaluated in alveolar macrophages (AMs) of bronchoalveolar lavage solution from pulmonary tuberculosis (PTB) patients and healthy volunteers respectively. Inhibitor experiment and promoter analysis were used to investigate the pathway involved in the induction of miR-146a. Examination of miR-146a function in macrophages was performed by overexpression and inhibition of miR-146a. Results: Among the altered miRNAs, 10 were downregulated whereas 8 were upregulated in M. bovis BCG-infected macrophage. MiR-146a was high expressed in cultured macrophage respond to M. bovis BCG but decreased in AMs of PTB patients, and stated a negative correlation with degree of smear-positive. Nuclear factor-κB pathway was required for the induction of miR-146a. Overexpression of miR-146a results in significant reduction of PTGS2 and enhanced the killing ability of THP-1 cells to intracellular M. bovis BCG, and miR-146a negatively regulated TNF-α release in feedback manner. Conclusions: Our findings suggest an important role of miR-146a in M. bovis BCG infection that helps to fine-tune the inflammation response of MTB infection. © 2014 The British Infection Association. Source


Zhang N.-Z.,The 97th Hospital of PLA | Bai S.,The 97th Hospital of PLA | Cai X.-J.,Southern Medical University | Li L.-B.,Southern Medical University
Photodiagnosis and Photodynamic Therapy | Year: 2016

Objective: To investigate the anti-tumor and immune efficacy of photodynamic immune-therapy (PIT), the combination of photodynamic therapy and dendritic cells (DC), on murine Heps hepatoma. Methods: DCs were derived from syngeneic mouse bone marrow and then labeled with DAPI in vitro. The hepatoma model was established by subcutaneous inoculation with Heps cells in one hundred and twenty-eight mice. They were then divided into four groups at random: control group, PDT group, DC group and PIT group. Tumors in the control group were injected with normal saline. Mice in the PDT group were injected with the photosensitizer Deuteporfin 24h before irradiation. Mice in the DC group were injected with DAPI labeled dendritic cells intratumorally. Mice in the PIT group were further given an injection of DCs after photoirradiation. Tumor growth and survival time were recorded after treatment. Fluorescence of tumor draining lymph nodes was evaluated under fluorescence microscope. Cytotoxic activity of splenocytes was tested by standard lactate dehydrogenase (lactate dehydrogenase, LDH) release assay. Results: (1) Tumor growth was significantly slowed down in the PDT and PIT group compared to the control group (P < 0.01). (2) The mean survival time was significantly prolonged in the PDT and PIT group. (3) The number of fluorescent cells in the draining lymph nodes from DC group was higher than that of the PIT group. (4) The anti-tumor activity of splenocytes in the PDT and PIT group was significantly higher than that of the DC and control groups (P < 0.01, P < 0.01). Conclusions: The present study suggests that PDT can inhibit tumor growth and induce anti-tumour immune response. The combination of PDT induced by Deuteporfiin and dendritic cell is capable of amplifying the antitumor immune response. © 2015 Elsevier B.V. Source


Shao G.-Q.,The 97th Hospital of PLA | Zhang N.-Z.,The 97th Hospital of PLA
World Chinese Journal of Digestology | Year: 2014

AIM: To observe the effect of somatostatin on the expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in subcutaneous xenografts derived from human gastric carcinoma SGC-7901 cells in nude mice. METHODS: An animal model of human gastric cancer xenograft was established by subcutaneously implanting SGC-7901 cells in nude mice. Twenty-four nude mice were randomly divided into four groups and treated with normal saline (group A), octreotide (group B), high-dose somatostatin (group C) and low-dose somatostatin (group D) for three weeks, respectively. After treatment, the animals were killed to take the tumors. The expression of VEGF and bFGF was examined by immunohistochemistry and Western blot. RESULTS: Immunohistochemical analysis indicated that the integral optical density (IOD)/ area (/pix2) of VEGF in groups A-D were 0.644 ± 0.022, 0.549 ± 0.002, 0.345 ± 0.019 and 0.435 ± 0.018, respectively. The expression of VEGF protein in tumor tissue was significantly higher in group A than in the other three groups (P < 0.001). Compared with group B, the expression of VEGF was reduced more significantly in groups C and D (P < 0.001 for both). The IOD/ area (/pix2) of bFGF in groups A-D were 0.723 ± 0.018, 0.558 ± 0.004, 0.288 ± 0.017 and 0.595 ± 0.011, respectively. The expression of bFGF protein in tumor tissue was significantly higher in group A than in the other three groups (P < 0.001). Compared with group B, the expression of bFGF was reduced more significantly only in group C (P < 0.001). Western blot analysis indicated that the relative expression of VEGF in groups A-D were 0.98 ± 0.02, 0.76 ± 0.02, 0.53 ± 0.01 and 0.53 ± 0.01, respectively. The relative expression of bFGF in groups A-D were 0.76 ± 0.02, 0.71 ± 0.02, 0.32 ± 0.01 and 0.51 ± 0.01, respectively. The expression of bFGF and VEGF protein was significantly higher in group A than in the other three groups (P < 0.001). Compared with group B, the expression of VEGF and bFGF was reduced more significantly in groups C and D (P < 0.001). CONCLUSION: Somatostatin can down-regulate the expression of VEGF and bFGF in subcutaneous xenografts derived from human gastric carcinoma SGC-7901 cells in nude mice, and the effect is more obvious than that of octreotide. © 2014 Baishideng Publishing Group Inc. All rights reserved. Source


Zhou J.,The 97th Hospital of PLA | Shi Y.,The 97th Hospital of PLA
Chinese Journal of Interventional Imaging and Therapy | Year: 2010

Objective: To evaluate the application value of trace subtraction fluoroscopy (TSF) in the interventional embolization of intracranial aneurysm. Methods: A total of 11 patients with intracranial aneurysm who underwent interventional embolization were enrolled in this study. Dual-source CT angiography (DSCTA) and digital subtraction angiography (DSA) were performed preoperatively in order to observe the size, shape, position of the aneurysm and measure the diameter of tumor neck and the whole tumor. TSF was used during operation. Results: All the 11 aneurysms of 11 patients were successfully embolized. Totally 39 guglielmi detachable coils were implanted, including 1 standard 3-D coil, 3 soft 3-D coils and 35 soft filling coils. Conclusion: The intraoperative application of TSF can improve the quality of the treatment for intracranial aneurysm patients. Source

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