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Jiang Y.-Q.,The 44th Hospital of PLA | Liu J.-C.,The 184th Hospital of PLA | Ye X.-J.,Orthopedics Hospital of Shanghai Changzheng Hospital | Hu Y.,The 44th Hospital of PLA | Qu J.-T.,The 44th Hospital of PLA
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Ligamentum flavum hypertrophy is one of the most important factors of lumbar spinal stenosis, but the molecular mechanism is still not very clear. OBJECTIVE: To explore the role of basic fibroblast growth factor, connective tissue growth factor and transforming growth factor β1 in hypertrophy of the lumbar ligamentum flavum. METHODS: The ligamentum flavum samples were divided into three groups according to different diseases: control group (acquired from the patients with lumbar spinal canal tumor, n=6), lumbar disc herniation (LDH) group (acquired from the patients with LDH, n=6) and lumbar spinal stenosis (LSS) group (acquired from the patients with LSS, n=6). Then the mRNA expressions of basic fibroblast growth factor, connective tissue growth factor, transforming growth factor β1 and collagen I, III, V of the ligamentum flavum were detected using real-time quantitative RT-PCR method. The roles of basic fibroblast growth factor, connective tissue growth factor and transforming growth factor β1 were explored. RESULTS AND CONCLUSION: The expression of basic fibroblast growth factor mRNA in the LSS group wassignificantly higher than that in the LDH and control groups (both P < 0.05); the expression of connective tissue growth factor mRNA was not found statistically different among the three groups, although it was slightly higher in the LSS group (P > 0.05); the expression of transforming growth factor β1 mRNA was significantly higher in the LSS group than in the LDH and control groups (both P < 0.01). The collagen I mRNA expressed significantly higher in the LSS group than the LDH and control groups (both P < 0.05), but both the collagen III and V mRNA showed no significant difference among the three groups (P > 0.05). This study indicate that both basic fibroblast growth factor and transforming growth factor β1 play important roles in the formation process of the lumbar ligamentum flavum hypertrophy, and the main type of the collagen in the hypertrophied ligamentum flavum is collagen I. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source


Chen J.,Tianjin Medical University | Han H.,Tianjin Medical University | Chen M.,The 44th Hospital of PLA | Xu X.-Z.,The Second People Hospital of Guizhou Province | And 2 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2014

Sendai virus strain Tianjin is a novel genotype. Here, we investigate the antitumor and proapoptotic effects of ultraviolet-inactivated Sendai virus strain Tianjin (UV-Tianjin) on human breast cancer MDA-MB-231 cells in vitro, as well as the involvement of the apoptotic pathway in the mechanism of UV-Tianjin-induced antitumor effects. MTT assays showed that treatment with UV-Tianjin dose-dependently inhibited the proliferation of MDAMB-231 cells but not normal MCF 10A breast epithelium cells. Hoechst staining and flow cytometric analysis revealed that UV-Tianjin induced apoptosis of MDA-MB-231 cells in a dose-dependent manner. Moreover, UV-Tianjin treatment resulted in reduction in the mitochondria membrane potential (MMP) and release of cytochrome complex (cyt c) via regulation of Bax and Bcl-2, as well as activation of caspase-9, caspase-3, Fas, FasL and caspase-8 in MDA-MB-231 cells. In summary, our study suggests that UV-Tianjin exhibits anticancer activity in human breast cancer MDA-MB-231 cells through inducing apoptosis, which may involve both the endogenous mitochondrial and exogenous death receptor pathways. Source


Li J.,General Hospital of Chengdu Military Command Area | Che J.-X.,The 44th Hospital of PLA | Bai S.-R.,General Hospital of Chengdu Military Command Area | Qiu Q.-M.,The 44th Hospital of PLA | And 3 more authors.
PLoS ONE | Year: 2013

Liver regeneration is the basic physiological process after partial hepatectomy (PH), and is important for the functional rehabilitation of the liver after acute hepatic injury. This study was designed to explore the effects of neurolytic celiac plexus block (NCPB) on liver regeneration after PH. We established a model of PH in rats, assessing hepatic blood flow, liver function, and serum CRP, TNF-α, IL-1β and IL-6 concentrations of the residuary liver after PH. Additionally, histopathological studies, immunohistochemistry, and western blotting were also performed. Our results indicated that NCPB treatment after PH improved liver regeneration and survival rates, increased hepatic blood flow, reduced hepatocyte damage, decreased the secretion and release of inflammatory cytokines, increased the expression of B cell lymphoma/leukemia-2 (Bcl-2), and decreased the expression of Bcl-2 associated X protein (Bax). Additionally, Western blotting revealed that the expression of NF-κB p65 and c-Jun were decreased in liver after NCPB. In conclusion, the results of our present study indicate that NCPB treatment has a favorable effect on liver regeneration after PH. We suggest that NCPB can be utilized as an effective therapeutic method to help the functional rehabilitation of the liver after acute hepatic injury or liver cancer surgery. © 2013 Li et al. Source

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