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Nie W.,The 425th Hospital of PLA | Meng L.,Shanghai JiaoTong University | Wang X.,Shanghai University | Xiu Q.,Shanghai University
Journal of Investigational Allergology and Clinical Immunology | Year: 2014

Background: Results regarding the association between the interferon-γ (IFN-γ) +874A/T polymorphism and asthma risk are controversial and ambiguous. The aim of this study was to determine with greater precision the relationship between the IFN-γ +874A/T polymorphism and asthma using a meta-analysis.Methods: Published literature was retrieved from 5 databases (PubMed, EMBASE, Wanfang, China National Knowledge Infrastructure [CNKI] and Weipu). ORs with 95% CIs were used to assess the strength of association.Results: Ten case-control studies involving 697 cases and 1049 controls were identified. In the overall analysis, a significant association between the +874A/T polymorphism and asthma susceptibility was found for AA vs AT + TT (OR, 1.89; 95% CI, 1.37-2.62; P=.0001). In the subgroup analysis by ethnicity, significant associations were found among whites (OR, 1.42; 95% CI, 1.04-1.93; P=.03) and Asians (OR, 2.52; 95% CI, 1.49-4.25; P=.0006). The sensitivity analysis and cumulative meta-analysis further strengthened the validity of this association. No publication bias was observed in this study.Conclusion: The results of this meta-analysis suggest that the IFN-γ +874A/T polymorphism is a risk factor for asthma. © 2014 Esmon Publicidad. Source


Wu X.,Second Military Medical University | Wu X.,Shanghai JiaoTong University | Tai Z.,Second Military Medical University | Zhu Q.,Shanghai University of Traditional Chinese Medicine | And 12 more authors.
International Journal of Nanomedicine | Year: 2014

Ligand-mediated prostate cancer (PCa)-targeting gene delivery is one of the focuses of research in recent years. Our previous study reported the successful preparation of aptamer-modified nanoparticles (APT-NPs) in our laboratory and demonstrated their PCa-targeting ability in vitro. However, the mechanism underlying this PCa-targeting effect and their anticancer ability in vivo have not yet been elucidated. The objective of this study was to assess the feasibility of using APT-NPs to deliver micro RNA (miRNA) systemically to PCa cells, to testify their tumor-targeting efficiency, and to observe their biodistribution after systemic administration to a xenograft mouse model of PCa. In addition, the effect of APT depletion and endocytosis inhibitors on cellular uptake was also evaluated quantitatively in LNCaP cells to explore the internalization mechanism of APT-NPs. Finally, blood chemistry, and renal and liver function parameters were measured in the xenograft mouse model of PCa to see whether APT-NPs had any demonstrable toxicity in mice in vivo. The results showed that APT-NPs prolonged the survival duration of the PCa tumor-bearing mice as compared with the unmodified NPs. In addition, they had a potential PCa-targeting effect in vivo. In conclusion, this research provides a prototype for the safe and efficient delivery of miRNA expression vectors to PCa cells, which may prove useful for preclinical and clinical studies on the treatment of PCa. © 2014 Wu et al. Source


Wu Z.,Jiaxing University | Zhan S.,Jiaxing University | Fan W.,The 425th Hospital of PLA | Ding X.,Shanghai JiaoTong University | And 10 more authors.
Nanoscale Research Letters | Year: 2016

Polyethylenimine (PEI) is considered to be a promising non-viral gene delivery vector. To solve the toxicity versus efficacy and tumor-targeting challenges of PEI used as gene delivery vector, we constructed a novel non-viral vector DR5-TAT-modified Pluronic-PEI (Pluronic-PEI-DR5-TAT), which was based on the attachment of low-molecular-weight polyethylenimine (LMW-PEI) to the amphiphilic polymer Pluronic to prepare Pluronic-modified LMW-PEI (Pluronic-PEI). This was then conjugated to a multifunctional peptide containing a cell-penetrating peptide (TAT) and a synthetic peptide that would bind to DR5—a receptor that is overexpressed in cancer cells. The vector showed controlled degradation, favorable DNA condensation and protection performance. The Pluronic-PEI-DR5-TAT/DNA complexes at an N/P ratio of 15:1 were spherical nanoparticles of 122 ± 11.6 nm and a zeta potential of about 22 ± 2.8 mV. In vitro biological characterization results indicated that Pluronic-PEI-DR5-TAT/DNA complexes had a higher specificity for the DR5 receptor and were taken up more efficiently by tumor cells than normal cells, compared to complexes formed with PEI 25 kDa or Pluronic-PEI. Thus, the novel complexes showed much lower cytotoxicity to normal cells and higher gene transfection efficiency in tumor cells than that exhibited by PEI 25 kDa and Pluronic-PEI. In summary, our novel, degradable non-viral tumor-targeting vector is a promising candidate for use in gene therapy. © 2016, Wu et al. Source


Chen D.,Central South University | Gao M.,Central South University | Fu Y.,Central South University | Xu X.,The 425th Hospital of PLA | Hao Z.,The 425th Hospital of PLA
Electrochimica Acta | Year: 2015

In this paper, a facile approach to manipulating osteogenic activity of orthopedic implants by in situ electrically controlled wettability was developed. Glycosaminoglycans (GAGs), the major organic extracellular matrix (ECM) components, were electrochemically doped in nanostructured poly(3,4-ethylenedioxythiophene) (NPEDOT) on Ti-6Al-4 V orthopedic implants. The controlled wettability of NPEDOT/GAGs was achieved by in situ applying electrical stimulus. Moreover, the in situ electrically controlled wettability could be utilized to implement the manipulation of osteogenic activity of orthopedic implants. This facile strategy may open up a new avenue to electrically responsive biomedical applications. © 2015 Elsevier Ltd. All rights reserved. Source


Wu X.,The 425th Hospital of PLA | Liu H.,The 425th Hospital of PLA
International Journal of Clinical and Experimental Medicine | Year: 2015

Associations between Obstructive sleep apnea/hypopnea syndrome (OSAHS) and risk of glaucoma remained controversial. Therefore, we performed this meta-analysis to investigate this association. We searched Pubmed, EMBASE, and Wangfang databases for studies before Oct. 10 2014. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to calculate the strength of association. A total of 12 studies with 36909 subjects on the association between OSAHS and glaucoma risk were included for this meta-analysis. OSAHS was associated with a significantly increased risk of glaucoma (OR = 1.65; 95% CI, 1.44-1.88; I2 = 43%). In the race subgroup analysis, both Asians (OR = 1.78; 95% CI, 1.49-2.12; I2 = 0%) and Caucasians (OR = 2.03; 95% CI, 1.12-3.69; I2 = 57%) with OSAHS had increased glaucoma risk. In the subgroup analysis according to gender, both women and men were significantly associated with risk of glaucoma (OR = 1.81; 95% CI, 1.27-2.57; I2 = 22% and OR = 1.62; 95% CI, 1.29-2.03; I2 = 0%, respectively). In the subgroup analysis by glaucoma type, OSAHS patients showed increased primary open-angle glaucoma (POAG) risk (OR = 1.87; 95% CI, 1.54-2.33; I2 = 0%) but not normal tension glaucoma (NTG) risk (OR = 3.57; 95% CI, 0.89-14.43; I2 = 0%). In addition, severe OSAHS patients had an increased glaucoma risk (OR = 5.49; 95% CI, 1.04-33.83; I2 = 0%), while mild and moderate OSAHS patients did not show significantly increased glaucoma risk. This meta-analysis suggested that the OSAHS may be a risk factor for glaucoma. © 2014, E-Century Publishing Corporation. All rights reserved. Source

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