The 359 Hospital of Chinese PLA

Zhenjiang, China

The 359 Hospital of Chinese PLA

Zhenjiang, China
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Chen Z.-X.,The 359 Hospital of Chinese PLA | Wang C.,Beihang University | Wang L.-J.,The Armed Police General Hospital
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Bone mass deficiency is common during implantation in the maxillary posterior region. Some patients do not want to accept extra surgery for increasing bone because of surgical trauma or medical expenses. Technology of the tilted implant has been proposed, but it has not been recognized and has a lack of relevant evidence. Objective: To investigate the stress distribution of per-implant bone which is subjected to different loading conditions when the different abutment angulations are applied, and to analyze the safety factor of different implants to verify the feasibility of the tilted implant in the maxillary posterior region. Methods: The finite element models of the different angulate abutments were established in commercial software packages. The safety factor of implant in the same power load was observed, and stress and strain condition of the implant and the surrounding bone was also observed. Results and Conclusion: With the increased abutment angulation, the maximum strain of pre-implant bone was significantly increased, and the safety factor of implant decreased gradually. Thus, the tilted implant is not recommended in the maxillary posterior region.


Weng Z.-Q.,Chinese PLA General Hospital | Sun M.,The 359 Hospital of Chinese PLA | Tang X.-Y.,Anhui Medical University | Wu Z.-A.,The 359 Hospital of Chinese PLA | And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Nano-calcium titanate, prepared with different modifiers, have properties of good osteogenesis, bone conductibility and biocompatibility. Objective: To observe the influence of nano-calcium titanate materials on the proliferation and differentiation of osteoblasts. Methods: Nano-calcium titanate was prepared with modifiers such as polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate. Meanwhile, nano-calcium titanate without modifier was prepared. All the four kinds of nano-calcium titanate materials were used to prepare nano-calcium titanate extracts under the concentration of 0.1, 1.0, 10 g/L, respectively. The proliferation of osteoblasts was observed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and alkaline phosphatase activity of osteoblasts was observed. Osteoblasts cultured in normal condition served as contrast. Results and Conclusion: The proliferation of osteoblasts cultured in the calcium titanate extracts prepared with polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate and without modifier was obviously improved under the concentration of 1.0 and 10 g/L. Absorbance value of the cells cultured in the above-mentioned four kinds of calcium titanate extracts 1.0 and 10 g/L) for 3, 6, 9 days exceeded the contrast (P < 0.05, P < 0.01), as well as the alkaline phosphatase activity (P < 0.05, P < 0.01). Calcium titanate prepared with polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate and without modifier has no cytotoxicity effect on osteoblasts, and can promote the proliferation and differentiation of osteoblasts.


PubMed | PLA Fourth Military Medical University and The 359 Hospital of Chinese PLA
Type: Journal Article | Journal: Spinal cord | Year: 2016

This is an experimental study.The objective of this study was to evaluate the neuroprotective effects of Ginkgo biloba extract 761 (EGb761) on histological features of injured sites and on functional performance of rats subjected to standardized spinal cord injury (SCI).This study was conducted in Xian, Shaanxi, China.Thirty female Sprague-Dawley rats were randomly divided into three groups: sham-operated, saline-treated control and EGb761-treated. The Basso, Beattie, Bresnahan Locomotor Rating Score (BBB score) was calculated and footprint analysis was performed to evaluate the functional performance of the rats in each group. Hematoxylin and eosin (HE) staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and caspase-3 staining were performed to evaluate the necrosis area and apoptotic cells at the injured site in each group.At 14, but not 1, 3 and 7, days post injury (DPI), rats in the EGb761-treated group exhibited significantly better BBB scores compared with the saline-treated control group (P<0.05). The EGb761-treated group also showed increased stride length, decreased stride width and reduced toe dragging at 14 DPI (P<0.05). Analysis of HE staining revealed that the EGb761-treated group had reduced necrosis at the injury site compared with the saline-treated control group (P<0.05). Analysis of TUNEL and caspase-3 staining demonstrated that cell apoptosis was increased at 1-14 DPI, peaking at 24-h post injury in the gray matter, and 7 DPI in the white matter. At 7 DPI, the quantity of apoptotic cells was significantly decreased in the EGb761-treated group.EGb761 administration during the acute phase after SCI significantly reduced secondary injury-induced tissue necrosis and cell apoptosis and improved functional performance in rats.


PubMed | PLA Fourth Military Medical University and The 359 Hospital of Chinese PLA
Type: Journal Article | Journal: Spinal cord | Year: 2014

To evaluate the effects of different doses of ascorbic acid (AA) on the functional performance of rats subjected to standardized spinal cord injury (SCI).Thirty female Sprague-Dawley rats were divided into three groups (10 animals in each group): control group: rats were subjected to SCI injury and received intraperitoneal saline administration; normal-dose AA group: rats were subjected to SCI injury and received daily intraperitoneal administration of AA at 100mgkg(-1) bodyweight; high-dose AA group: rats were subjected to SCI injury and received daily intraperitoneal administration of AA at 200mgkg(-1) bodyweight. The Basso, Beattie, Bresnahan Locomotor Rating Score (BBB score) and footprint analysis were performed to evaluate the functional performance of the rats in each group, and hematoxylin and eosin staining was performed to evaluate necrosis at the injury site.At days 14 and 28 after SCI, rats in the high-dose AA group, but not the normal-dose AA group, exhibited significantly better BBB score compared with the control group (P<0.05). Compared with the control and normal-dose AA group, the high-dose AA group also showed increased stride length, decreased stride width and reduced toe dragging (P<0.05). Histological analysis revealed that both the normal- and high-dose AA groups had reduced necrosis in the injury site compared with the control group (P<0.05).High-dose AA administration during the acute phase post SCI significantly reduced secondary injury-induced tissue necrosis and improved functional performance in rats.


Fan A.-M.,The 359 Hospital of Chinese PLA | He Z.,The 359 Hospital of Chinese PLA | Zhang L.-L.,The 359 Hospital of Chinese PLA | Feng T.-C.,The 359 Hospital of Chinese PLA | Liu F.-G.,The 359 Hospital of Chinese PLA
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: The calcaneus is cancellous bone with thin cortical bone, and mostly prone to collapse compression fracture after injuries. Surgical reduction and fixation can basically restore joint integrity and calcaneus form, patients often need bone graft to fill the bone and receive Kirschner wire fixation to support bone graft and maintain reduction. OBJECTIVE: To explore the advantages and defects of small incision reduction graft and percutaneous Kirschner wire fixation in treatment of calcaneal fractures. METHODS: 26 patients with calcaneal fractures were treated with small incision reduction graft (autograft or allograft bone) plus percutaneous Kirschner wire fixation, and clinical data of the involved patients were retrospectively analyzed. Bohler and Gissane angles of calcaneal bone were observed after fixation. Maryland foot scoring system was applied to evaluate the clinical efficacy of bone graft and fixation, soft tissue complications after fixation were also observed. RESULTS AND CONCLUSION: All 26 patients were followed up for 6-12 months. Compared with before surgery, calcaneus Bohler and Gissane angles were significantly increased after treatment; according to the Maryland foot scores, excellent and good rate was 85%. Small incision reduction graft and percutaneous Kirschner wire fixation is reliable treatment method of intra-articular calcaneal fractures, with fewer complications. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Wu Z.-a.,The 359 Hospital of Chinese PLA | Xuan Z.-g.,The 359 Hospital of Chinese PLA | Wang C.-f.,The 359 Hospital of Chinese PLA | Liu S.,The 359 Hospital of Chinese PLA | Sun M.,The 359 Hospital of Chinese PLA
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011

BACKGROUND: Nanosized hydroxyapatite with different size and morphology has different surface performance and bioactivity. Different sized nanohydroxyapatite was synthesized via surface modification of hydroxyapatite; by this method, the application of nanosized hydroxyapatite can be extended. OBJECTIVE: To analyze the effect of experimental parameters, such as type of organic modifiers and pH value, etc. on the size of hydroxyapatite synthesized by hydrothermal method. METHODS: Hydroxyapatite with different sizes was synthesized starting from phosphoric acid and calcium nitrate and using D-sorbitol, sodium polyacrylate and Tween 80 as organic modifiers at different pH values. RESULTS AND CONCLUSION: In the absence of organic modifier, the size of hydroxyzpatite was decreased rapidly with the increase in pH values. The presence of sodium polyacrylate was beneficial to the formation of small-size hydroxapatite. The addition of D-sorbitol favored the formation of large-size hydroxyapatite. The presence of Tween 80 decreased the size of hydroxyapatite in some extent.

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