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Yang F.,Bayannur City Hospital | Shi B.,The 309th Hospital of PLA | Cao L.,The 309th Hospital of PLA
Experimental and Therapeutic Medicine | Year: 2017

The aim of the present study was to compare and observe the expression levels of vascular endothelial growth factor (VEGF) and miRNA-17-5p during the treatment of seawater-immersed blast-injury wounds (SIBIW) under different conditions of vacuum sealing drainage (VSD), and to identify the optimized range of VSD treatment and partially explain its mechanisms. The bilateral hips and scapulae of experimental pigs (weight, 25-30 kg) were subjected to blast-injury wounds, followed by the seawater immersion. The animals then underwent conventional dressing treatment under 120, 180 and 240 mmHg VSD. Visual observation, in addition to histological, immunohistochemical and molecular biological techniques were applied to compare and observe the extent of wound healing and expression levels of VEGF and miRNA-17-5p. The wound healing of the VSD treatment group was improved compared with the control group, with 120 mmHg negative pressure producing the most marked effect. miR-17-5p expression was detected in the SIBIW granulation tissues. There was significant difference between each VSD treatment group and control group at each time point (P<0.05). Thus, the present results show that miR-17-5p can be expressed in SIBIW granulation tissues, and this effect is most evident under 120 mmHg negative pressure, which may inform the optimized negative range for the treatment of SIBIW. © 2017, Spandidos Publications. All rights reserved.


Wu S.,The 302nd Hospital of PLA | Ding Y.,Shanghai University | Wu F.,The 309th Hospital of PLA | Xie G.,The 302nd Hospital of PLA | And 2 more authors.
Journal of Psychiatry and Neuroscience | Year: 2016

Background: We conducted a systematic review and meta-analysis to determine the association between serum lipid levels and suicidality, as evidence from previous studies has been inconsistent. Methods: We identified relevant studies by searching Medline, Web of Science, EMBASE, and the Cochrane Database of Systematic Reviews (1980 to Dec. 5, 2014). Studies assessing the association between serum total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and/or triglycerides (TG) levels and suicidality were included. We used a random-effects model to take into account heterogeneity among studies. Results: We included 65 studies with a total of 510 392 participants in our analysis. Compared with the nonsuicidal patients, suicidal patients had signifi­cantly lower serum TC (weighted mean difference [WMD] –22.35, 95% confidence interval [CI] –27.95 to –16.75), LDL-C (WMD –19.56, 95% CI –26.13 to –12.99) and TG (WMD –23.40, 95% CI –32.38 to –14.42) levels, while compared with the healthy controls, suicidal patients had significantly lower TC (WMD –24.75, 95% CI –27.71 to –21.78), HDL-C (WMD –1.75, 95% CI –3.01 to –0.48) and LDL-C (WMD –3.85, 95% CI –7.45 to –0.26) levels. Furthermore, compared with the highest serum TC level category, a lower serum TC level was associated with a 112% (95% CI 40%–220%) higher risk of suicidality, including a 123% (95% CI 24%–302%) higher risk of suicide attempt and an 85% (95 CI 7%–221%) higher risk of suicide completion. The cut-off values for low and high serum TC level were in compliance with the categories reported in the original studies. Limitations: A major limitation of our study is the potential heterogeneity in most of the analyses. In addition, the suicidal behaviour was examined using different scales or methods across studies, which may further explain het­erogeneity among the studies. Conclusion: We identified an inverse association between serum lipid levels and suicidality. More mechanistic studies are needed to further explain this association. © 2016 8872147 Canada Inc.


Yang B.,The 309th Hospital of PLA | Cao L.,University of Aberdeen | Liu B.,The 309th Hospital of PLA | McCaig C.D.,University of Aberdeen | Pu J.,University of Aberdeen
PLoS ONE | Year: 2013

Breaking the balance between proliferation and differentiation in animal cells can lead to cancer, but the mechanisms maintaining this balance remain largely undefined. The calcium activated chloride channel A1 (CLCA1) is a member of the calcium sensitive chloride conductance family of proteins and is expressed mainly in the colon, small intestine and appendix. We show that CLCA1 plays a functional role in differentiation and proliferation of Caco-2 cells and of intestinal tissue. Caco-2 cells spontaneously differentiate either in confluent culture or when treated with butyrate, a molecule present naturally in the diet. Here, we compared CLCA1 expressional levels between patients with and without colorectal cancer (CRC) and determined the functional role of CLCA1 in differentiation and proliferation of Caco-2 cells. We showed that: 1) CLCA1 and CLCA4 expression were down-regulated significantly in CRC patients; 2) CLCA1 expression was up-regulated in Caco-2 cells induced to differentiate by confluent culture or by treatment with sodium butyrate (NaBT); 3) Knockdown of CLCA1 with siRNA significantly inhibited cell differentiation and promoted cell proliferation in Caco-2 confluent cultures, and 4) In Caco-2 3D culture, suppression of CLCA1 significantly increased cell proliferation and compromised NaBT-induced inhibition of proliferation. In conclusion, CLCA1 may contribute to promoting spontaneous differentiation and reducing proliferation of Caco-2 cells and may be a target of NaBT-induced inhibition of proliferation and therefore a potential diagnostic marker for CRC prognosis. © 2013 Yang et al.


Fei W.,The 309th Hospital of PLA | Hong W.,The 309th Hospital of PLA
Advanced Materials Research | Year: 2014

Current traditional physiological monitoring is accomplished by individual cardiogram monitor device attached to each patient's bed. A nurse will have to frequently monitor the vital signs at patient's bedside, which is inefficient and time consuming. Although some hospitals have deployed the central monitoring system, it still has space limit to the nurses and high cost. Our research work is to develop a mobile nurse information system with efficient physiological monitoring for patient based on iPad. Patient's real-time physiological signals captured by the physiological monitoring system are integrated to the mobile nurse information system. Nurses could get just-in-time access to the data of the physiological monitoring system at anywhere through the clinical use of the mobile nurse information system. Trial version in our hospital showed that the system is feasible and efficient enough to apply in clinical settings. © (2014) Trans Tech Publications, Switzerland.


Liu Z.,The 309th Hospital of PLA | Zhou G.,The 309th Hospital of PLA | Deng X.,The 97th Hospital of PLA | Yu Q.,The 309th Hospital of PLA | And 6 more authors.
Journal of Infection | Year: 2014

Objectives: The regulatory mechanism of microRNA (miRNA) within macrophage innative response to Mycobacterium tuberculosis infection is not clear yet. Methods: The expression profile of cellular miRNAs during Mycobacterium bovis BCG infection was analyzed by using microarray. The expression of miR-146a was evaluated in alveolar macrophages (AMs) of bronchoalveolar lavage solution from pulmonary tuberculosis (PTB) patients and healthy volunteers respectively. Inhibitor experiment and promoter analysis were used to investigate the pathway involved in the induction of miR-146a. Examination of miR-146a function in macrophages was performed by overexpression and inhibition of miR-146a. Results: Among the altered miRNAs, 10 were downregulated whereas 8 were upregulated in M. bovis BCG-infected macrophage. MiR-146a was high expressed in cultured macrophage respond to M. bovis BCG but decreased in AMs of PTB patients, and stated a negative correlation with degree of smear-positive. Nuclear factor-κB pathway was required for the induction of miR-146a. Overexpression of miR-146a results in significant reduction of PTGS2 and enhanced the killing ability of THP-1 cells to intracellular M. bovis BCG, and miR-146a negatively regulated TNF-α release in feedback manner. Conclusions: Our findings suggest an important role of miR-146a in M. bovis BCG infection that helps to fine-tune the inflammation response of MTB infection. © 2014 The British Infection Association.


Wu F.,The 309th Hospital of PLA | Wang H.,The 309th Hospital of PLA
Applied Mechanics and Materials | Year: 2014

This paper has presented a mobile clinical information system for hospital ward rounds based on iPad. We use Service-Oriented Architecture (SOA) infrastructure together with web service technology to make the distributed and fragmented medical data accessible through mobile devices. Trial version in our hospital showed that the system is feasible and efficient enough to apply in clinical settings. © (2014) Trans Tech Publications, Switzerland.


Jia C.,the 309th Hospital of PLA
Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns | Year: 2016

Although the extraordinary agent wound is not common, the difficulties of its diagnosis, treatment, and the high medical risk as well as the indeterminacy of its prognosis bring great challenges to the clinicians. It is mainly attributed to the complexities of extraordinary agent wounds and the deficiency in the assessment technic of wound. Therefore, it is necessary and important to establish a precise assessment method to benefit the surgical planning, pre-estimation of peri-operative risk, and the doctor-patient communication. Based on the relative scientific research and our recent clinical research data, we bring forth our opinions on the current status and the development trend of the assessment of extraordinary agent wounds in this article.


Chen Y.P.,the 309th Hospital of PLA
Zhonghua nei ke za zhi [Chinese journal of internal medicine] | Year: 2010

To analyze the clinical characteristics of myasthenia gravis (MG) associated with thyroid abnormalities. A total of 300 MG patients admitted to the department of neurology from July 2008 to September 2009 were reviewed retrospectively. Based on the thyroid function and abnormality of thyroid related antibodies (thyroglobulin antibody and peroxidase antibody), the patients with MG were divided into two groups (260 cases without thyroid disease and 40 cases with thyroid abnormalities). The different clinical features, the relationship between the antithyroid antibodies and anti-acetylcholine receptor antibody (AChRAb) and the distribution of T cell subsets between the two groups were compared. (1) Among the 300 MG patients, 13.3% of them was accompanied with thyroid disease and the most common abnormality was positive thyroid antibody. (2) Between the groups of MG with and without thyroid abnormalities, no significant differences on the sex percentage, age of onset, duration of disease, clinical classification and thymic abnormalities were found (P>0.05). (3) The blood levels of AChRAb in postsynaptic membrane in the thyroid antibody-positive patients were 1.15±0.11, being much higher than those in the antibody-negative patients (1.01±0.11). (4) The percentage of CD8+ T cells was significantly decreased [(21.63±5.17)% vs (24.28±5.79)%] and the ratio of CD4+/CD8+ T cells was increased (2.10±0.67 vs 1.81±0.61, P<0.05) in the group of MG with thyroid abnormality as compared with the group of MG with normal thyroid. In MG patients the incidence positive thyroid related antibodies was much higher than that of other thyroid abnormalities. It is suggested that there are higher levels of AChRAb in MG coexisting with positive thyroid antibodies and more abnormalities of T lymphocyte subset distribution in MG patients with thyroid disease.


Huang X.W.,the 309th Hospital of PLA
Zhongguo gu shang = China journal of orthopaedics and traumatology | Year: 2011

To evaluate the effectiveness of open reduction and internal fixation and repair of palmar ligment in treating trans-scaphoid perilunate dislocation. From June 1995 to June 2001,14 patients with trans-scaphoid perilunate dislocation were treated with open reduction and internal fixation and repair of palmar ligment. Among them,there were 13 males and 1 female,the ranging in age from 21 to 38 years,averaged 25.4 years. All patients were posterior dislocation and all operations were performed within 2 weeks after injury. All patients were followed up from 24 to 60 months with an average of 28.3 months. Thirteen scaphoid fractures were primary healed and functions of wrist joint were good. Bone disunion was found in 1 case and part functions of wrist joint were limited. No found necrosis of lunate and scaphoid. According to clinical scoring system of Cooney, 9 case got excellent results, 3 good, 1 fair and 1 poor. Open reduction and internal fixation and repair of palmar ligament is effective in treating trans-scaphoid perilunate dislocation,which can early provide steady fixation for scaphoid,and profit to recover blood supply of lunatum and subterminal scaphoid.


Zhu C.,The 309th Hospital of PLA
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2013

To identify specific antigens related to streptomycin resistant (SMr) Mycobacterium tuberculosis. Cellular proteins were extracted from SMr clinical isolate 01108, SM-sensitive clinical isolate 01105 and H37Rv. Differential expression proteins were identified with isobaric tags for relative and absolute quantitation (iTRAQ) combined with Nano LC-MS/MS technology. Approximately 194 and 146 differential expression proteins were identified in 01108 strain compared with the proteomic profiles of 01105 strain and H37Rv, respectively, and 121 proteins were identified in 01108 strain compared with the proteomic profiles of both 01105 strain and H37Rv. Identified proteins showed a pI (isoelectric point) variation between 3.74-12.48 and a molecular mass (M) range between 7.63 and 326.2 kDa. Differential expression proteins were mainly associated with metabolism (involved in intermediary metabolism, respiration, and lipid metabolism) and took part in catalysis and binding function. Seven ribosomal proteins (Rv0056, Rv0641, Rv0652, Rv0701, Rv1630, Rv2442c and Rv2785c) and seven proteins (the ratios > 1.20 or < 0.55) were commonly down-regulated in 01108 strain compared with both 01105 strain and H37Rv, i. e. the thiol peroxidase (Rv1932), acyl carrier protein dehydrogenase (Rv0824c), 30S ribosomal protein S15 (Rv2785c), acetone acid dehydrogenase E2 part (Rv2215), two-component transcriptional regulatory protein (Rv3133c) and Hypothetical protein (Rv2466e and Rv2626c). Differential expression proteins were found in SMr strain compared with both SM-sensitive strain and H37Rv. Further studies are needed to assess the role of these differential expression proteins in SM resistance.

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