Entity

Time filter

Source Type


Zhu C.,PLA Fourth Military Medical University | Zhao Y.,309th Hospital of the Chinese PLA | Zhang Z.,PLA Fourth Military Medical University | Ni Y.,PLA Fourth Military Medical University | And 2 more authors.
Molecular Medicine Reports | Year: 2015

MicroRNAs (miRNAs) are short, non-coding RNAs that are aberrantly expressed in tumors. miRNA-33a (miR-33a) is closely associated with cholesterol metabolism and is essential for cellular growth. The aim of the present study was to explore the role of miR-33a and identify its clinical significance in lung cancer cells. miR-33a was observed to be overexpressed in the lung cancer cell lines A549 and NCI-H460. MTT assay results demonstrated that the overexpression of miR-33a significantly inhibited the proliferation of A549 cells, and similar results were obtained from the colony formation assay. This suggests that transfection of miR-33a may suppress the growth of lung cancer cells. Overexpression of miR-33a was also observed to result in marked G1/S phase cell cycle arrest in A549 and NCI-H460 cell lines using fluorescence-activated cell sorting analysis. Western blot analysis revealed that overexpression of miR-33a significantly reduced the expression of β -catenin in A549 and NCI-H460 cells, suggesting a direct or indirect regulation of β-catenin by miR-33a in lung cancer cells. In conclusion, the current study may provide strategies for the treatment of lung cancer and clarify the mechanism of its progression. Source


Cao B.-S.,309th Hospital of the Chinese PLA | Wu J.-H.,306th Hospital of the Chinese PLA | Li X.-L.,309th Hospital of the Chinese PLA | Deng J.,309th Hospital of the Chinese PLA | Liao G.-Q.,309th Hospital of the Chinese PLA
Journal of Ultrasound in Medicine | Year: 2011

Objectives - The purpose of this study was to assess the impact of contrast-enhanced sonography on sonographically guided transthoracic needle biopsy of lung lesions. Methods - A total of 121 patients underwent sonographically guided transthoracic needle cutting biopsy. Of the 121 patients, 62 (contrast-enhanced sonography group) underwent contrast-enhanced sonography before biopsy, and the information from contrast-enhanced sonography was used to optimize the biopsy procedure. The remaining 59 patients constituted the non-contrast-enhanced sonography group. The enhancement patterns and echogenicity were evaluated by the consensus of 2 sonographers. The diagnostic efficacy was compared between the contrast-enhanced and non-contrast-enhanced sonography groups. Results - The enhancement intensity and extent varied greatly among different thoracic lesions, and an anechoic area (necrosis) was revealed in 26 of 62 lesions (41.9%) lesions after administration of the contrast agent. The overall diagnostic accuracy of sonographically guided transthoracic biopsy in this study was 85.9% (104 of 121). In the contrast-enhanced sonography group, the initial biopsy led to correct diagnosis in 58 of 62 lesions (93.6%). In the non-contrast-enhanced sonography group, the initial biopsy led to correct diagnosis in 46 of 59 lesions (78.0%). The difference in the diagnostic accuracy between the contrast-enhanced and non-contrast-enhanced sonography groups was statistically significant (P < .05). Conclusions - Contrast-enhanced sonography enables differentiation of viable from necrotic portions of thoracic lesions and has a positive impact on the diagnostic efficacy of sonographically guided transthoracic needle biopsy. © 2011 by the American Institute of Ultrasound in Medicine. Source


Cao B.-S.,309th Hospital of the Chinese PLA | Liang Y.-M.,309th Hospital of the Chinese PLA | Li X.-L.,309th Hospital of the Chinese PLA | Deng J.,309th Hospital of the Chinese PLA | Zhang G.-C.,309th Hospital of the Chinese PLA
Journal of Ultrasound in Medicine | Year: 2013

Objectives-The purpose of this study was to describe the findings of juxtapleural pulmonary tuberculoma on contrast-enhanced sonography and investigate their correlation with histologic findings. Methods-From April 2008 to April 2012, 21 patients with biopsy or clinically proven juxtapleural pulmonary tuberculomas underwent contrast-enhanced sonography with an intravenous bolus injection of 4.8 mL of a sulfur hexafluoride-filled microbubble contrast agent. Enhancement patterns and functional parameters (time to enhancement, time to peak enhancement, and peak signal intensity) derived from a time-intensity curve were evaluated. Enhancement patterns were correlated with their histologic findings. Results-A rim enhancement pattern was presented in 12 (57.1%), a homogeneous enhancement pattern in 5 (23.8%), and a heterogeneous enhancement pattern in 4 (19.1%) of 21 tuberculomas. A pathologic study confirmed that the nonenhancing center of the rim enhancement pattern corresponded to caseous or liquefied necrosis, and homogeneously enhanced portions corresponded to granulomatous inflammation. The medians (25th-75th interquartile ranges) for the time to enhancement, time to peak enhancement, and peak signal intensity were 14 seconds (9-14 seconds), 22 seconds (21-26 seconds), and 83 dB (55-92 dB), respectively. Conclusions-Contrast-enhanced sonography of juxtapleural pulmonary tuberculoma is feasible. Juxtapleural pulmonary tuberculomas usually show rim, homogeneous, or heterogeneous enhancement. Enhancement patterns of juxtapleural pulmonary tuberculomas are well correlated with their pathologic features. © 2013 by the American Institute of Ultrasound in Medicine. Source

Discover hidden collaborations