PubMed | Chinese PLA General Hospital, Beijing Institute of Pharmacology and Toxicology and the 309 Hospital of Chinese PLA
Type: Journal Article | Journal: Neural regeneration research | Year: 2015
Status epilepticus was induced via intraperitoneal injection of lithium-pilocarpine. The inhibitory effects of propofol on status epilepticus in rats were judged based on observation of behavior, electroencephalography and 24-hour survival rate. Propofol (12.5-100 mg/kg) improved status epilepticus in a dose-dependent manner, and significantly reduced the number of deaths within 24 hours of lithium-pilocarpine injection. Western blot results showed that, 24 hours after induction of status epilepticus, the levels of N-methyl-D-aspartate receptor 2A and 2B subunits were significantly increased in rat cerebral cortex and hippocampus. Propofol at 50 mg/kg significantly suppressed the increase in N-methyl-D-aspartate receptor 2B subunit levels, but not the increase in N-methyl-D-aspartate receptor 2A subunit levels. The results suggest that propofol can effectively inhibit status epilepticus induced by lithium-pilocarpine. This effect may be associated with downregulation of N-methyl-D-aspartate receptor 2B subunit expression after seizures.
PubMed | Institute for Tuberculosis Research and the 309 Hospital of Chinese PLA
Type: Journal Article | Journal: Annals of clinical and laboratory science | Year: 2015
The identification and characterization of antigens of Mycobacterium tuberculosis help in understanding the mechanisms of protective immunity and in improving diagnostic methods for TB. Rv0057 and Rv1352 proteins are new T-cell antigens, found to play roles in TB infection. In this study, a recombinant fusion protein Rv0057-Rv1352 was made and analyzed for its immunological characteristics and potential utility. It showed good immunoreactivity with serum from TB patients by western blotting. The antibody levels against Rv0057-Rv1352 were significantly higher in sera from 69 TB patients than in sera from 60 patients with non-TB respiratory diseases (P<0.001). The sensitivities of a diagnostic ELISA test based on detecting Rv0057-Rv1352 antibody (60.3%) or 38 kDa-16 kDa antibody (58.8%) were comparable to commercial rapid test B (75.4%), and significantly higher (p<0.001) than bacteriological methods (15.9%), rapid test A (20.3%), or rapid test C (29.0%). The specificities of Rv0057-Rv1352 (93.3%) or 38 kDa-16 kDa antibody tests (93.3%) were equivalent to the rapid tests A (93.3%) and C (86.7%), and significantly higher than rapid test B (63.3%, p<0.001). When 38 kDa-16 kDa was used together with Rv0057-Rv1352, the test sensitivity reached 85.5%, and its specificity remained high (86.7%). The test was as sensitive with bacterium-positive TB patients as with bacterium-negative. In an ELISPOT assay for cellular immunity, Rv0057-Rv1352 stimulated T lymphocytes to produce fewer spots secreting IFN- than CFP10-ESAT6 fusion protein did (p>0.05). These results suggest that Rv0057-Rv1352 has potential for the serodiagnosis of active pulmonary TB.
Liang T.,the 309 Hospital of Chinese PLA |
Cai M.,the 309 Hospital of Chinese PLA |
Li Z.-L.,the 309 Hospital of Chinese PLA |
Shi B.-Y.,the 309 Hospital of Chinese PLA |
And 3 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010
BACKGROUND: The number of living donor kidney transplantation is greater than that of cadaveric kidney transplantation in some hospitals, however, the safety, superiority, surgical techniques, as well as the ethical issues of living donor kidney transplantation needs further explored. OBJECTIVE: To analyze the outcomes of kidney transplantation with different sources of kidney by reviewing clinical experience of the living donor and cadaveric donor kidney transplantation at the same periods. METHODS: Totally 65 cases received living donor kidney transplantation and 169 cases received cadaveric kidney transplantation from January 2002 to March 2008 in the Organ Transplantation Center, 309 Hospital of Chinese PLA, were collected. The data of recipients with different kidney sources, including gender, age, human leucocyte antigen-matching, surgery status, complications, immunosuppression scheme, kidney function and long-term follow-up were comparatively studied to clarify the clinical significance of living donor kidney transplantation. RESULTS AND CONCLUSION: All 65 cases of living donor kidney transplantation were successful, and the 1-year patient/graft survival rate was 100%. Cadaveric donor kidney transplantation was successful, 2 of which died of cerebral hemorrhage disease, 6 died of severe lung infection after 1 year, and the 1-year patient/graft survival rate was 95.26%. The living donor kidney transplantation was superior to the cadaveric donor kidney transplantation in complications and 1-year patient/graft survival rate and other factors. Living donor kidney transplantation provides a reliable means for patients with full preparation, good matching, few postoperative complications, and high patient/graft survival rate.
Cui X.,The 309 Hospital of Chinese PLA |
Ma Y.-Z.,The 309 Hospital of Chinese PLA |
Li D.-W.,The 309 Hospital of Chinese PLA |
Xue H.-B.,The 309 Hospital of Chinese PLA
Chinese Journal of Tissue Engineering Research | Year: 2013
BACKGROUND: The degradation rate and strength of poly(lactic-co-glycolic acid) (PLGA) can be regulated by the addition of beta-tricalcium phosphate (β-TPC). OBJECTIVE: To test the effect of β-TPC/PLGA/isoniazid (INH)/levofloxacin (LVFX) composite on the repair of femoral condyle bone defects in rabbits. METHODS: Thirty New Zealand rabbits were used for preparing a rabbit model of bone defects with the diameter of 5 mm and the depth of 10 mm. The rabbits were randomly divided into three groups: group 1, group 2 and group 3. The former two groups were implanted with β-TPC/PLGA and β-TPC/PLGA/INH/LVFX, respectively. The group 3 was untreated as the blank control group. The repair effects of each group were detected by imaging, gross specimen and histological examination. RESULTS AND CONCLUSION: At week 12 after treatment, bone defects in the two experimental groups were both radiographically repaired, and there was no significant difference between these two groups in the ratio of new bone area to bone defects area (P > 0. 05). The blank control group failed to restore bone defects. These results suggest that the PLGA/TCP/INH/LVFX material can effectively repair femoral condyle bone defects in rabbits.
Ma C.,Xuzhou Medical College |
Xu Z.,The 309 Hospital of Chinese PLA |
Wang Z.-Q.,The 309 Hospital of Chinese PLA |
Deng S.-Y.,Xuzhou Medical College
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: Intervention using known inflammatory transmitters has limitations on relieving secondary spinal cord injury. Interleukin-17 is an important proinflammatory cytokine, and is gradually paid attention in the pathogenesis of central nervous system diseases. OBJECTIVE: To investigate the altered rule of interleukin-17 mRNA and protein in a rat model of acute spinal cord injury. METHODS: Healthy male Sprague-Dawley rats were randomly assigned to two groups. In the model group, rats were made into complete spinal cord transaction models. In the sham surgery group, only spinal dura mater was opened, but parenchyma was not injured. Basso, Beattie, Bresnahan locomotor rating scale was used to observe the effects of acute spinal cord injury on limb motor function of rats. Hematoxylin-eosin staining was used to observe histopathological changes at various time points after spinal cord injury. Real-time fluorescence quantitative PCR and western blotting were used to detect interleukin-17 mRNA and protein levels in each group at various time points after spinal cord injury. RESULTS AND CONCLUSION: Basso, Beattie, Bresnahan locomotor rating scale: Basso, Beattie, Bresnahan scores were 20 to 21 in the sham surgery group. Basso, Beattie, Bresnahan scores were 0 at 1 and 2 days after spinal cord injury. At 7 days, Basso, Beattie, Bresnahan scores were 0 to 3 (P < 0.05). Hematoxylin-eosin staining results revealed that compared with the sham surgery group, inflammatory cell infiltration, neuronal and glial cell swelling, and a reduced number of neuronal processes were observed at 6 hours after spinal cord injury. Gray matter and white matter were loose and vacuolated at 12 hours. Gliocyte proliferation and tissue fibrosis were apparent at 7 days. Real-time PCR results demonstrated that interleukin-17 mRNA appeared at 3 hours, and peaked at 6 hours (P < 0.01), and then decreased. Interleukin-17 mRNA levels were similar to the sham surgery group at 7 days. Western blotting results revealed that interleukin-17 expression began to increase at 6 hours and peaked at 12 hours (P < 0.05), and then reduced, and reached the levels in the sham surgery group at 7 days. Results indicated that tissue injury was most severe at 12 hours, and showed a time consistency with interleukin-17 expression. It is inferred that interleukin-17 is possibly involved in the process of secondary inflammatory reaction of spinal cord.
Li N.,The 309 Hospital of Chinese PLA |
Wang X.,The 309 Hospital of Chinese PLA |
Zhai J.,The 309 Hospital of Chinese PLA |
Shi Y.,The 309 Hospital of Chinese PLA |
And 6 more authors.
Biomedical Research (India) | Year: 2014
Background: thymidine phosphorylase (TP) has been identified as a specific marker enzyme that is expressed in tumor-infiltrating macrophages, being associated with tumor angiogenesis and poor prognosis in patients with intestinal-type gastric cancer. However, the clinical and prognostic significance of TP expression in gastric cancer remains controversial. Publications were identified which assessed the clinical or prognostic significance of TP expression in gastric cancer from January 1995 to May 2013. A meta-analysis was performed to clarify the association between TP expression and clinical outcomes. A total of 13 studies met the inclusion criteria, and comprised 1541 cases. Analysis of these data showed that there was no statistically significant association of TP and tumor differentiation (pooled OR = 0.88, 95% CI: 0.67-1.15, Z = 0.94, P = 0.95, fixed-effect). There was significant association between the expression of TP and clinical parameters such as lymph node metastasis (pooled OR = 2.35, 95% CI: 1.42-3.88, Z = 3.34, P = 0.0009 random-effect) or lymphatic invasion (pooled OR = 1.71, 95% CI: 1.02-2.87, Z = 2.02, P = 0.04 random-effect). Moreover, in identified studies, the expression of TP was correlated with reduced overall survival (relative risk [RR] = 1.73, 95% CI: 1.06-2.82, Z = 2.19, P = 0.03). TP expression is associated with tumor aggressiveness and poor prognosis. If this finding is confirmed by well-designed prospective studies, TP expression may be a useful prognostic indicator for clinical outcome in patients with gastric cancer.
PubMed | the 309 Hospital of Chinese PLA
Type: Journal Article | Journal: Neural regeneration research | Year: 2014
To analyze international research trends in hepatic encephalopathy and examine the role of neuroelectrophysiology and neuroimaging in diagnosis of hepatic encephalopathy.We performed a bibliometric analysis of studies on hepatic encephalopathy published during 2002-2011 retrieved from Web of Science.(1) peer-reviewed published articles on hepatic encephalopathy; (2) original article, review, meeting abstract, proceedings paper, book chapter, editorial material, news items, and (3) published during 2002-2011.(1) articles that required manual searching or telephone access; (2) documents that were not published in the public domain; and (3) corrected papers from the total number of articles.(1) Annual publication output; (2) type of publication; (3) publication by research field; (4) publication by journal; (5) publication by author; (6) publication by institution; (7) publication by country; (8) publication by institution in China; (9) most-cited papers.A total of 3 233 papers regarding hepatic encephalopathy were retrieved during 2002-2011. The number of papers gradually increased over the 10-year study period and was highest in 2010. Most papers appeared in journals with a focus on gastroenterology and hepatology. Among the included journals, Hepatology published the greatest number of papers regarding hepatic encephalopathy, and the published studies were highly cited. Thus, Hepatology appears to represent a key journal publishing papers on hepatic encephalopathy. Regarding distribution by country for publications on hepatic encephalopathy indexed in Web of Science during 2002-2011, the United States published highest number of papers, with China ranked ninth. As per distribution by institute for publications, the University of Montreal in Canada published the highest number of papers (n = 111). Among the Chinese institutes, Zhejiang University in China was the most prolific institute with 15 papers.The present bibliometric analysis on hepatic encephalopathy provides an overview of research progress, as well as identifying the most active institutes and experts in this research field during 2002-2011. Research into hepatic encephalopathy has revealed changes in neural injury and regeneration in hepatic encephalopathy. Neuroelectrophysiological and neuroimaging examinations are important for determining clinical classifications and disease severity of hepatic encephalopathy, providing a foundation for further research.