Ma C.,Xuzhou Medical College |
Xu Z.,The 309 Hospital of Chinese PLA |
Wang Z.-Q.,The 309 Hospital of Chinese PLA |
Deng S.-Y.,Xuzhou Medical College
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: Intervention using known inflammatory transmitters has limitations on relieving secondary spinal cord injury. Interleukin-17 is an important proinflammatory cytokine, and is gradually paid attention in the pathogenesis of central nervous system diseases. OBJECTIVE: To investigate the altered rule of interleukin-17 mRNA and protein in a rat model of acute spinal cord injury. METHODS: Healthy male Sprague-Dawley rats were randomly assigned to two groups. In the model group, rats were made into complete spinal cord transaction models. In the sham surgery group, only spinal dura mater was opened, but parenchyma was not injured. Basso, Beattie, Bresnahan locomotor rating scale was used to observe the effects of acute spinal cord injury on limb motor function of rats. Hematoxylin-eosin staining was used to observe histopathological changes at various time points after spinal cord injury. Real-time fluorescence quantitative PCR and western blotting were used to detect interleukin-17 mRNA and protein levels in each group at various time points after spinal cord injury. RESULTS AND CONCLUSION: Basso, Beattie, Bresnahan locomotor rating scale: Basso, Beattie, Bresnahan scores were 20 to 21 in the sham surgery group. Basso, Beattie, Bresnahan scores were 0 at 1 and 2 days after spinal cord injury. At 7 days, Basso, Beattie, Bresnahan scores were 0 to 3 (P < 0.05). Hematoxylin-eosin staining results revealed that compared with the sham surgery group, inflammatory cell infiltration, neuronal and glial cell swelling, and a reduced number of neuronal processes were observed at 6 hours after spinal cord injury. Gray matter and white matter were loose and vacuolated at 12 hours. Gliocyte proliferation and tissue fibrosis were apparent at 7 days. Real-time PCR results demonstrated that interleukin-17 mRNA appeared at 3 hours, and peaked at 6 hours (P < 0.01), and then decreased. Interleukin-17 mRNA levels were similar to the sham surgery group at 7 days. Western blotting results revealed that interleukin-17 expression began to increase at 6 hours and peaked at 12 hours (P < 0.05), and then reduced, and reached the levels in the sham surgery group at 7 days. Results indicated that tissue injury was most severe at 12 hours, and showed a time consistency with interleukin-17 expression. It is inferred that interleukin-17 is possibly involved in the process of secondary inflammatory reaction of spinal cord.
Cui X.,The 309 Hospital of Chinese PLA |
Ma Y.-Z.,The 309 Hospital of Chinese PLA |
Li D.-W.,The 309 Hospital of Chinese PLA |
Xue H.-B.,The 309 Hospital of Chinese PLA
Chinese Journal of Tissue Engineering Research | Year: 2013
BACKGROUND: The degradation rate and strength of poly(lactic-co-glycolic acid) (PLGA) can be regulated by the addition of beta-tricalcium phosphate (β-TPC). OBJECTIVE: To test the effect of β-TPC/PLGA/isoniazid (INH)/levofloxacin (LVFX) composite on the repair of femoral condyle bone defects in rabbits. METHODS: Thirty New Zealand rabbits were used for preparing a rabbit model of bone defects with the diameter of 5 mm and the depth of 10 mm. The rabbits were randomly divided into three groups: group 1, group 2 and group 3. The former two groups were implanted with β-TPC/PLGA and β-TPC/PLGA/INH/LVFX, respectively. The group 3 was untreated as the blank control group. The repair effects of each group were detected by imaging, gross specimen and histological examination. RESULTS AND CONCLUSION: At week 12 after treatment, bone defects in the two experimental groups were both radiographically repaired, and there was no significant difference between these two groups in the ratio of new bone area to bone defects area (P > 0. 05). The blank control group failed to restore bone defects. These results suggest that the PLGA/TCP/INH/LVFX material can effectively repair femoral condyle bone defects in rabbits.
Li N.,The 309 Hospital of Chinese PLA |
Wang X.,The 309 Hospital of Chinese PLA |
Zhai J.,The 309 Hospital of Chinese PLA |
Shi Y.,The 309 Hospital of Chinese PLA |
And 6 more authors.
Biomedical Research (India) | Year: 2014
Background: thymidine phosphorylase (TP) has been identified as a specific marker enzyme that is expressed in tumor-infiltrating macrophages, being associated with tumor angiogenesis and poor prognosis in patients with intestinal-type gastric cancer. However, the clinical and prognostic significance of TP expression in gastric cancer remains controversial. Publications were identified which assessed the clinical or prognostic significance of TP expression in gastric cancer from January 1995 to May 2013. A meta-analysis was performed to clarify the association between TP expression and clinical outcomes. A total of 13 studies met the inclusion criteria, and comprised 1541 cases. Analysis of these data showed that there was no statistically significant association of TP and tumor differentiation (pooled OR = 0.88, 95% CI: 0.67-1.15, Z = 0.94, P = 0.95, fixed-effect). There was significant association between the expression of TP and clinical parameters such as lymph node metastasis (pooled OR = 2.35, 95% CI: 1.42-3.88, Z = 3.34, P = 0.0009 random-effect) or lymphatic invasion (pooled OR = 1.71, 95% CI: 1.02-2.87, Z = 2.02, P = 0.04 random-effect). Moreover, in identified studies, the expression of TP was correlated with reduced overall survival (relative risk [RR] = 1.73, 95% CI: 1.06-2.82, Z = 2.19, P = 0.03). TP expression is associated with tumor aggressiveness and poor prognosis. If this finding is confirmed by well-designed prospective studies, TP expression may be a useful prognostic indicator for clinical outcome in patients with gastric cancer.