Wang J.D.,The 307th Hospital of PLA
Zhongguo gu shang = China journal of orthopaedics and traumatology | Year: 2011
To explore the relationship of motor dysfunction of the lower extremities with the imaging appearances and clinical features of metastatic epidural spinal cord compression (MESCCs). From July 2006 to December 2007, 26 successive patients with metastases of the thoracic, lumbar and the cervical spine were treated in our department. Forty-three main involved vertebra in all 26 patients were evaluated by magnetic resonance imaging and computed tomography, and were scored according motor dysfunction in this study. Fourteen patients (25 vertebrae) had motor dysfunction. Among 26 patients, 12 cases with visceral metastasis,in which had motor dysfunction in 10 cases; 14 cases without visceral metastasis, in which had motor dysfunction in 4 cases; comparison between two groups, P=0.0079. Among vertebral presence of continuity of 43 main involved vertebrae, 16 vertebrae had motor dysfunction;among vertebral absence of continuity, motor dysfunction occurred in 9 vertebrae, comparison between two groups, P=0.1034. Among vertebral presence of lamina involvement of 43 main involved vertebrae, 11 vertebrae had motor dysfunction; among vertebral absence of lamina involvement, motor dysfunction occurred in 14 vertebrae, comparison between two groups, P=0.020 5. Among vertebral presence of protruding of vertebral posterior wall of 43 main involved vertebrae, 12 vertebrae had motor dysfunction; among vertebral absence of protruding of vertebral posterior wall, 13 vertebrae had motor dysfunction, comparison between two groups, P=0.0334. Among vertebral presence of involvement epidural space of 43 main involved vertebrae, 11 vertebrae had motor dysfunction; among vertebral absence of involvement epidural space, 14 vertebrae had motor dysfunction, comparison between two groups, P=0.003 6. Such factors as age, gender, whether or not received regular chem before admission, back pain degree of metastasis, received regular chem before admission, therapeutic efficacy of primary tumor, number of bony metastases outside spine, number of the main involved vertebrae, level of vertebral metastases location, level of continuous involved vertebrae, vertebral-body involvement, fracture of anterior column, fracture of posterior wall, and pedicle involvement had no effects on incidence of motor dysfunction due to MESCC (P>0.05). MESCC with visceral metastases, lamina involvement, presence of outstanding buttocks sign of posterior wall,involvement epidural space tended to cause symptomatic MESCC. Incidence of continuity of main involved vertebrae occurred more frequently in the CUTS compared with other levels of spine.
Liu C.-B.,The 307th Hospital of PLA |
Lu S.-H.,The 307th Hospital of PLA |
Zhang X.-Z.,The 307th Hospital of PLA
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: Culture condition, isolation method and efficiency of porcine bone marrow mesenchymal stem cells are different, and there is a lack of unified identification standards. OBJECTIVE: To establish a high-efficiency and economical method of isolating, culturing and identifying porcine bone marrow mesenchymal stem cells. METHODS: The bone marrow was collected from healthy porcine femur to isolate mononuclear cells by Ficoll density gradient centrifugation method. After primary and subculture, the cell surface antigens were detected by flow cytometery, and the osteogenic, adipogenic and chondrogenic differentiation abilities of the cells were observed. RESULTS AND CONCLUSION: The isolated mononuclear cells showed exuberant viability, and grew well in vitro. They were spindle- or small polygonal-shaped and had a stable doubling time on culture. Flow cytometric analysis revealed that they were positive for CD29, CD44, CD90, and negative for CD14, CD34, CD45, CD166, HLA-DR. Furthermore, they were able to differentiate into osteogenic, adipogenic and chondrogenic lineages under specific conditions in vitro. Porcine bone marrow mesenchymal stem cells isolated by Ficoll density gradient centrifugation method can be purified, grow well in vitro, and have multi-lineage differentiation potentials, which can act as seed cells for tissue engineering research. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
Feng K.,Organ Transplantation Center |
Xu Y.-W.,Organ Transplantation Center |
Ye F.-G.,Organ Transplantation Center |
Jiang M.,The 307th Hospital of PLA |
And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: Recent studies have shown that the large-dose regular insulin therapy used to control blood glucose levels can cause 50% of patients suffering from vascular, optic nerve and kidney complications. Previous results from authors exhibit that when allogeneic hematopoietic stem cell transplantation is applied for treatment of leukemia, diabetic symptoms in patients disappear. Dose it prompt that allogeneic hematopoietic stem cell transplantation is an effective therapy for treatment of diabetes mellitus? OBJECTIVE: To explore the feasibility of hematopoietic stem cell transplantation for treatment of diabetes mellitus. METHODS: A retrospective analysis was done regarding the data of patients with hematological diseases complicated with diabetes mellitus who underwent allogenetic hematopoietic stem cell transplantation. Four patients with acute lymphocyte leukemia, chronic myelogenous leukemia, aplastic anemia, and myolodysplastic syndromes, respectively, were complicated with diabetes mellitus. Conditioning regimen was cyclophosphamide+ total body irradiation protocol. Cyclosporin A and short-term methotrexate were used for graft-versus-host disease prophylaxis. Blood glucose was controlled by oral hypoglycemic drugs or insulin injections before transplantation. RESULTS AND CONCLUSION: All the four patients were successfully engrafted. Fasting glucose level of the four patients recovered at 4-6 months after hematopoietic stem cell transplantation (without hypoglycemic drugs). One patient died of leukemia relapse after 12 months of hematopoietic stem cell transplantation. The other three patients had disease-free survival until the time of follow-up. © 2015 Journal of Clinical Rehabilitative Tissue Engineering Research, All rights reserved.
Hou C.-Y.,The 307th Hospital of PLA |
Xu L.-L.,The 307th Hospital of PLA |
Xu L.-L.,Anhui Medical University |
Chen H.,The 307th Hospital of PLA |
And 6 more authors.
Medical Science Monitor | Year: 2013
Background: We aimed to guide clinical nursing by studying the relationship between intestinal acute graft-versus-host disease and intestinal infection after hematopoietic stem cell transplantation. Material/Methods: We present an effective nursing method by comparing and analyzing the degree, duration time, and volume of diarrhea, and the distribution of pathogens in 44 patients who developed intestinal aGVHD after hematopoietic stem cell transplantation (24 patients with no intestinal infection). Results: 21.4% of patients with grade I-II intestinal aGVHD developed into intestinal infection and 87.5% of patients with grade III-IV intestinal aGVHD developed into intestinal infection (P<0.05). Higher mortality was found in the grade III-IV intestinal aGVHD patients with intestinal infection. Patient age had no effect on the incidence of GVHD according to our data (P<0.05). We found remarkable differences in the amount and duration of diarrhea between patients with and without intestinal infection (P<0.05). The most common pathogens cultivated were Candida glabrata (24%) and Candida albicans (22.67%). Conclusions: The incidence of intestinal infection increased remarkably after intestinal aGVHD occurred. Severe aGVHD can easily lead to fungus infection. Nursing care can decrease the incidence of intestinal infection in aGVHD. © Med Sci Monit.
Wu T.-T.,The 309th Hospital of PLA |
Li H.-C.,The 307th Hospital of PLA |
Zheng F.,The 309th Hospital of PLA |
Ao G.-K.,The 309th Hospital of PLA |
And 2 more authors.
CardioVascular and Interventional Radiology | Year: 2016
Purpose: The Habib™ VesOpen Catheter is a new endovascular radiofrequency ablation (RFA) device used to treat malignant portal obstruction. The purpose of this study was to evaluate the clinical feasibility and safety of RFA with this device. Methods: We collected the clinical records and follow-up data of patients with malignant portal obstruction treated with percutaneous endovascular portal RFA using the Habib™ VesOpen Catheter. Procedure-related complications, improvement of symptoms, portal patency, survival, and postoperative biochemical tests were investigated. Results: The 31 patients enrolled in the study underwent 41 successful endovascular portal RFA procedures. Patients were divided into a portal-stenting (PS) group (n = 13), which underwent subsequent portal stenting with self-expandable metallic stents, and a non-stenting (NS) group (n = 18), which did not undergo stenting. No procedure-related abdominal hemorrhage or portal rupture occurred. Postablation complications included abdominal pain (n = 26), fever (n = 13), and pleural effusion (n = 15). Improvements in clinical manifestations were observed in 27 of the 31 patients. Of the 17 patients experiencing portal restenosis, 10 underwent successful repeat RFA. The rate of successful repeat RFA was significantly higher in the NS group than in the PS group. Median portal patency was shorter in the PS group than in the NS group. No mortality occurred during the 4 weeks after percutaneous endovascular portal RFA. Conclusions: Percutaneous endovascular portal RFA is a feasible and safe therapeutic option for malignant portal obstruction. Prospective investigations should be performed to evaluate clinical efficacy, in particular, the need to evaluate the necessity for subsequent portal stenting. © 2016, Springer Science+Business Media New York and the Cardiovascular and Interventional Radiological Society of Europe (CIRSE).