The 306th Hospital of PLA
The 306th Hospital of PLA
Xu L.,Beijing Normal University |
Zhang H.,CAS Shenzhen Institutes of Advanced Technology |
Zhang H.,Peking University |
Hui M.,Beijing Normal University |
And 4 more authors.
Neuroscience | Year: 2014
Motor execution and imagery (ME and MI), as the basic abilities of human beings, have been considered to be effective strategies in motor skill learning and motor abilities rehabilitation. Neuroimaging studies have revealed several critical regions from functional activation for ME as well as MI. Recently, investigations have probed into functional connectivity of ME; however, few explorations compared the functional connectivity between the two tasks. With betweenness centrality (BC) of graph theory, we explored and compared the functional connectivity between two finger tapping tasks of ME and MI. Our results showed that using BC, the key node for the ME task mainly focused on the supplementary motor area, while the key node for the MI task mainly located in the right premotor area. These results characterized the connectivity patterns of ME and MI and may provide new insights into the neural mechanism underlying motor execution and imagination of movements. © 2013 IBRO.
Shen K.,Beijing Childrens Hospital |
Xiong T.,IMS Health |
Tan S.C.,IMS Health |
Wu J.,The 306th Hospital of PLA
PloS one | Year: 2016
BACKGROUND: Influenza is a common viral respiratory infection that causes epidemics and pandemics in the human population. Oseltamivir is a neuraminidase inhibitor-a new class of antiviral therapy for influenza. Although its efficacy and safety have been established, there is uncertainty regarding whether influenza-like illness (ILI) in children is best managed by oseltamivir at the onset of illness, and its cost-effectiveness in children has not been studied in China.OBJECTIVE: To evaluate the cost-effectiveness of post rapid influenza diagnostic test (RIDT) treatment with oseltamivir and empiric treatment with oseltamivir comparing with no antiviral therapy against influenza for children with ILI.METHODS: We developed a decision-analytic model based on previously published evidence to simulate and evaluate 1-year potential clinical and economic outcomes associated with three managing strategies for children presenting with symptoms of influenza. Model inputs were derived from literature and expert opinion of clinical practice and research in China. Outcome measures included costs and quality-adjusted life year (QALY). All the interventions were compared with incremental cost-effectiveness ratios (ICER).RESULTS: In base case analysis, empiric treatment with oseltamivir consistently produced the greatest gains in QALY. When compared with no antiviral therapy, the empiric treatment with oseltamivir strategy is very cost effective with an ICER of RMB 4,438. When compared with the post RIDT treatment with oseltamivir, the empiric treatment with oseltamivir strategy is dominant. Probabilistic sensitivity analysis projected that there is a 100% probability that empiric oseltamivir treatment would be considered as a very cost-effective strategy compared to the no antiviral therapy, according to the WHO recommendations for cost-effectiveness thresholds. The same was concluded with 99% probability for empiric oseltamivir treatment being a very cost-effective strategy compared to the post RIDT treatment with oseltamivir.CONCLUSION: In the Chinese setting of current health system, our modelling based simulation analysis suggests that empiric treatment with oseltamivir to be a cost-saving and very cost-effective strategy in managing children with ILI.
Qin Y.,Chongqing Medical University |
Qin Y.,Chengdu Military General Hospital |
Li G.-L.,Chengdu Military General Hospital |
Xu X.-H.,Chengdu Military General Hospital |
And 4 more authors.
Behavioural Brain Research | Year: 2016
Mild traumatic brain injury (mTBI) or concussion is a common health issue. Several people repeatedly experience head impact milder than that causing concussion. The present study aimed to confirm the effects of such repeated impact on the brain structure and cognitive abilities. Rat models were established by closed skull weight-drop injury. The animals were anesthetized, subjected to single (s)-sham, s-mTBI, repetitive (r)-sham, and r-mTBI, and recovery times were recorded. MRI, including T2-weighted and diffusion tensor imaging (DTI), as well as, neurological severity scores (mNSS) were assessed for the dynamics of the brain structure and neurological function. Morris water maze (MWM) was used to evaluate the cognitive function. The histological examination of r-mTBI rats revealed the basis of structural changes in the brain. There was no significant difference in the recovery time, MRI, mNSS, and MWM between the s-sham and the s-mTBI groups. Compared with r-sham, r-mTBI induced significant differences in the following aspects. The recovery time was prolonged and beam balance test (BBT) in mNSS increased from day 5. MWM performances were worse even after the BBT was recovered. The volumes of the cortex (CT), hippocampus (HP), and lateral ventricle had changed from day 5, which reached a maximum at day 14. Abnormal DTI parameters were observed in CT, corpus callosum, and HP. Histological analyses showed that both in CT and HP, neuron counts reduced at the end of the experiment. Altogether, these findings indicate that non-symptomatic head injury may result in brain atrophy and cognitive impairment when occurred repeatedly. © 2016 Elsevier B.V.
Xia X.,Chengdu Military General Hospital |
Qu B.,Chengdu Military General Hospital |
Li Y.-M.,Chengdu Military General Hospital |
Yang L.-B.,Chengdu Military General Hospital |
And 6 more authors.
Journal of Molecular Neuroscience | Year: 2017
Nuclear factor of activated T cells (NFAT) is a multifunctional cytokine family. NFAT5 was recently reported to be involved in many neuronal functions, but its specific function remains unclear. In this study, our aim is to investigate whether NFAT5 overexpression can protect astrocytes against oxygen–glucose–serum deprivation/restoration (OGSD/R) damage. In vivo, rats were subjected to ischemia–reperfusion injury, resulting in increased water content, infarct volume, and expression of NFAT5 protein in rat spinal cord. After primary culture for spinal cord astrocytes, the in vitro OGSD/R model was established. The results of the CCK8 assay and flow cytometry showed that, in the OGSD/R group, astrocyte cell viability was downregulated, but astrocyte apoptosis increased. Caspase 3 activity increased as well. Levels of NFAT5, as detected by real-time quantitative PCR and western blot, decreased under OGSD/R, as did SIRT1. Commercial kits for activity assays were used to show that OGSD/R inhibited SIRT1 activation but accelerated SOD activation after OGSD/R. Next, pcDNA-NFAT5 or NFAT5 siRNA was transfected into astrocytes. Overexpression of NFAT5 not only promoted the survival of the astrocytes and SIRT1 activation under OGSD/R but also inhibited cell apoptosis and SOD activation. Moreover, overexpression of NFAT5 apparently diminished histone acetylation and promoted the nuclear transport of Nrf2. Our results show that NFAT5 protects spinal astrocytes in a manner that depends on activation of the SIRT1/Nrf2 pathway. These findings present a novel potential molecular mechanism for NFAT5 therapy in the context of spinal cord injury. © 2016, Springer Science+Business Media New York.
Fan C.-S.,Beijing Medical and Health Economic Research Association |
He M.-M.,Beijing University of Chinese Medicine |
Wu J.-H.,The 306th Hospital of PLA
Chinese Journal of New Drugs | Year: 2017
Objective: To evaluate the possible impact of adding Oral Tetra-Arsenic Tetra-Sulfide Formula into national basic medical insurance formula on medical insurance expenditure. Methods: Based on the budget impact analysis guide published by International Society for Pharmacoeconomics and Outcomes Research, a span of 5 years model was constructed by Excel. The incidence of acute promyelocytic leukemia came from literature, and direct medical cost and other data were extracted from hospital patient medical cost information database. The replacement reimbursement ratio of Oral Tetra-Arsenic Tetra-Sulfide Formula added in to formula was based on expert opinion. Results: If Oral Tetra-Arsenic Tetra-Sulfide Formula was brought into national basic medical insurance formula from 2018 to 2022, the total annual costs of arsenic in the 5 years would increase by 3.598 6 million, 7.197 2 million, 10.795 8 million, 14.394 4 million and 17.993 million RMB, respectively. The total annual medical costs in the 5 years would decrease by 2.663 6 million, 5.327 2 million, 7.990 9 million, 10.654 5 million and 13.318 1 million RMB, respectively, and the total annual saving costs of medical insurance in the 5 years were 1.359 2 million, 2.718 4 million, 4.077 6 million, 5.436 8 million and 6.796 million RMB, respectively. Sensitivity analysis was conducted based on the change of the incidence of acute promyelocytic leukemia, the replacement rate of arsenic injection by Oral Tetra-Arsenic Tetra-Sulfide Formula after included in formula and the change of drug price. The result indicated that adding Oral Tetra-Arsenic Tetra-Sulfide Formula into national basic medical insurance formula was beneficial for medical insurance expense saving. Conclusion: Adding Oral Tetra-Arsenic Tetra-Sulfide Formula into national basic medical insurance formula will optimize the allocation of resources. © 2017, Chinese Journal of New Drugs Co. Ltd. All right reserved.
Jin E.,Peking University |
Luo L.,The 306th Hospital of PLA |
Bai Y.,Peking University |
Zhao M.,Peking University
Annals of Pharmacotherapy | Year: 2015
Background: Bevacizumab and triamcinolone acetonide (TA) are both common choices for treatment of diabetic macular edema (DME), but the comparative efficacy of combined or separate applications is still not determined. Objectives: To compare the treatment efficacy of intravitreal bevacizumab (IVB) and the combination of IVB and intravitreal triamcinolone (IVT) for DME patients. Methods: PubMed, EMBASE, and the Cochrane library were systematically reviewed for randomized controlled trials comparing IVB with IVB/IVT. Data on visual acuity (VA) and central macular thickness (CMT) changes at 3 and 6 months were extracted and data on adverse events were collected. A meta-analysis was performed using the software RevMan 5.3. The methodological quality and bias risks were also evaluated. Results: VA improved more significantly in the IVB/IVT group compared with the IVB group at 3 months (mean difference [MD] = 0.07; 95% CI = 0.01 to 0.13), whereas there was no significant difference at 6 months (MD = −0.01; 95%CI = −0.11 to 0.09). The CMT reduction in the IVB/IVT group was significantly greater than that in the IVB group at 3 months (MD = 48.40; 95%CI = 30.23 to 66.57), but no statistically significant difference was found at 6 months (MD = 0.47; 95%CI = −24.11 to 25.04). Ocular hypertension was detected in 9/243 eyes in the IVB/IVT group but none of the IVB eyes. Conclusion: IVB/IVT is more effective for improving VA and decreasing CMT at 3 months in DME. A single injection of TA along with the first IVB could improve outcome within 3 months, but this is not sustained at 6 months. Continuous IVT/IVB treatment should be performed in further trials to clarify its long-term potential efficacy. © The Author(s) 2015
Cao W.,China Agricultural University |
Chen W.,China Agricultural University |
Liang X.,China Agricultural University |
Zhou J.,The 306th Hospital of PLA |
And 3 more authors.
American Journal of Reproductive Immunology | Year: 2014
Problem: Epididymitis, one of the most common urological diseases, can lead to the destruction of the epididymal duct and cause transient or permanent sterility. The aim of this study was to investigate the functions and related mechanisms of all trans retinoic acid (atRA) in alleviating the acute inflammation of epididymitis. Method of study: The mouse model of the epididymitis was induced by injecting Escherichia coli into the cauda epididymis. atRA was administrated for five consecutive days through intraperitoneal injection. The expression levels of inflammatory cytokines were measured by real-time PCR and Western blot. In addition, cultured primary mouse epididymal epithelial cells were treated with different concentrations of atRA and RAR antagonists to identify whether the effect of atRA was mediated through RAR. Results: Our results demonstrate that atRA ameliorates the inflammation in mouse epididymitis by decreasing the expression of the pro-inflammatory cytokines and increasing the expression of anti-inflammatory factors including TGF-β1 and IL-10. Our results show that the upregulating effect of atRA on TGF-β1 was mediated by RARα, and the enhancing effect of atRA on IL-10 expression was mediated via RARβ. Conclusion: These new results suggest that atRA is involved in regulating the inflammatory response of epididymis. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Liang W.,Shaoxing Peoples Hospital |
Gao B.,The 306th Hospital of PLA |
Xu G.,Shaoxing Peoples Hospital |
Weng D.,Shaoxing Peoples Hospital |
And 2 more authors.
Cell Biochemistry and Biophysics | Year: 2014
The aim of this study was to investigate associations between single nucleotide polymorphisms rs13301537 in asporin (ASPN) and rs373444 in the bone morphogenetic protein 5 (BMP5) gene with knee osteoarthritis (OA) susceptibility in a Chinese Han population. ASPN rs13301537 and BMP5 rs373444 polymorphisms were genotyped in patients with knee OA and age- and sex-matched OA-free controls from a Chinese Han population. A total of 510 patients with knee OA and 520 controls were enrolled in the study. CT and CC genotypes of rs13301537, and variant C, were associated with a significantly increased risk of knee OA. On stratification analysis, the association between the risk of OA and rs13301537 CT heterozygotes compared with TT homozygotes was stronger in females and those aged >65 years. In contrast, the CT and CC genotypes of rs373444 in BMP5 were not significantly associated with the risk of knee OA, even after further stratification analysis according to age or sex. Our results showed that ASPN rs13301537 T to C change and variant C genotype may contribute to knee OA risk in a Chinese Han population. © 2014, Springer Science+Business Media New York.
Ma W.M.,The 306th hospital of PLA
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2011
To study the change of the blood coagulation function of guinea pigs exposed to 16 Hz/120 dB, 16 Hz/125 dB infrasound and to explore the mechanism of circulation system damage. Seventy-two guinea pigs were divided into 3 groups: the control group, the group exposed to 16 Hz/120 dB infrasound for 1.5 h a day and the group exposed to 16 Hz/125 dB infrasound for 1.5 h a day. Each exposure group was divided into 4 sub-groups (8 guinea pigs a sub-group) which were exposed to infrasound for 1, 7, 14 and 21 d, respectively. The coagulation function and serum nitric oxide (NO) were measured for control group and all sub-groups after exposure to infrasound. The prothrombin time (PT), international normalized ratio (INR) and serum NO of group exposed to 16 Hz/125 dB infrasound were (31.16 ± 3.05) s, 2.53 ± 1.21 and (88.304 ± 52.601) μmol/L, respectively, which were significantly higher than those [(21.36 ± 0.10) s, 1.65 ± 0.07 and (30.943 ± 26.864) μmol/L] of control group (P < 0.05). PT and INR of sub-groups exposed to 16 Hz/125 dB infrasound for 14 and 21 d were significantly higher than those of control group. NO of sub-groups exposed to 16 Hz/125 dB infrasound for 1 week and 2 weeks were significantly higher than that of control group (P < 0.05), but NO of sub-group exposed to 16 Hz/125 dB infrasound for 3 weeks decreased slightly. The blood coagulation function of guinea pigs exposed to 16 Hz/125 dB infrasound decreased, PT and INR may be used as the indexes to assess of blood coagulation function change induced by the infrasound exposure.
Wang R.J.,The 306th Hospital of PLA
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2012
To explore the expression of phosphorylated STAT3 (pSTAT3) in non-small cell lung cancer(NSCLC) and its clinical significance. The expression of pSTAT3 was detected in 59 cases of NSCLC and their para-cancerous tissue by immunohistochemical staining. (1) The expression of pSTAT3 in NSCLC was significantly higher than that in para-cancerous tissue. (2) The expression of pSTAT3 was associated with tumor's size and smoking status, which was significantly higher in smaller tumors(diameter<3 cm) and nonsmokers than that in larger tumors(diameter≥3 cm) and smokers, but was not associated with gender, age, lymph node metastasis, clinical stage and differentiation grade. The percentage rate of pSTAT3 expression was higher in adenocarcinoma than that in squamous cell carcinoma but without statistically significant. pSTAT3 may play an important role in the development of NSCLC and the direction of targeted therapy.