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Beijing, China

Xie X.,Zhengzhou University | Wang L.,Peking University | Yao M.,Peking University | Wen X.,Peking University | And 5 more authors.
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology

OBJECTIVE: To investigate the changes in red blood cell count in patients with different liver diseases and the correlation between red blood cell count and degree of liver damage.METHODS: The clinical data of 1427 patients with primary liver cancer, 172 patients with liver cirrhosis, and 185 patients with hepatitis were collected, and the Child-Pugh class was determined for all patients. The differences in red blood cell count between patients with different liver diseases were retrospectively analyzed, and the correlation between red blood cell count and liver function status was investigated. The Mann-Whitney U test, Kruskal-Wallis H test, rank sum test, Spearman rank sum correlation test, and chi-square test were performed for different types of data.RESULTS: Red blood cell count showed significant differences between patients with chronic hepatitis, liver cancer, and liver cirrhosis and was highest in patients with chronic hepatitis and lowest in patients with liver cirrhosis (P < 0.05). In the patients with liver cirrhosis, red blood cell count tended to decrease in patients with a higher Child-Pugh class (P < 0.05).CONCLUSION: For patients with liver cirrhosis, red blood cell count can reflect the degree of liver damage, which may contribute to an improved liver function prediction model for these patients. Source

Zhang Y.,The 302 Hospital of PLA | Kong H.-L.,Capital Medical University | Zheng S.-J.,Capital Medical University | Liu M.,Capital Medical University | And 3 more authors.
World Chinese Journal of Digestology

AIM: To investigate the effect of lentiviral-mediated delivery of shRNA targeting the SMAD3 gene on liver regeneration in rats. METHODS: Sixty male rats were randomly divided into three groups: SMAD3 shRNA group (n = 20), shRNA control group (n = 20), and normal saline group (n = 20). Intrasplenic injection of lentivirus carrying the SMAD3 shRNA or control shRNA at a dose of 1.0 × 108 TU per rat was performed in rats of the SMAD3 shRNA group and shRNA control group, respectively, while the normal saline group was given equal volume of normal saline. After 96 h, 2/3 partial hepatectomy was performed to develop a rat model of liver regeneration. Seven rats of each group were sacrificed at 96 h, and the rest were sacrificed at 144 h after hepatectomy. Liver tissue specimens were collected. SMAD3 expression was detected using real-time PCR and immunohistochemistry. Proliferation of hepatocytes was evaluated by detecting Ki67 by immunohistochemistry. The ratio of liver weight to body weight was determined to observe its role in liver regeneration in vivo. RESULTS: At 96 h and 144 h after hepatectomy, SMAD3 mRNA expression decreased by 73% and 63% in the SMAD3 shRNA group compared to the shRNA control group. The expression of SMAD3 protein also decreased obviously. More active proliferation of hepatocytes was observed after SMAD3 down-regulation. The liver weight/body weight in the SMAD3 shRNA group was higher than those in the normal saline control group and shRNA control group (96 h: 4.50 ± 0.43 vs 3.97 ± 0.55 vs 3.98 ± 0.40, 144 h: 4.66 ± 0.54 vs 4.15 ± 0.51 vs 4.20 ± 0.34), but there was no statistically significance between them (all P > 0.05). CONCLUSION: Down-regulation of SMAD3 expression could moderately promote liver regeneration in rats. © 2012, WJC Press. All rights reserved. Source

Zhang D.K.,The 302 Hospital of PLA
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology

To investigate the diagnostic value of acoustic radiation force impulse (ARFI) imaging technology for the assessment of liver fibrosis in chronic hepatitis C (CHC) patients. One-hundred-and-eight CHC patients were examined by real-time ultrasound elastography using the Acuson S2000 ARFI instrument (Siemens Healthcare) and underwent liver biopsy for pathohistological analysis. The correlation between liver fibrosis grades determined by the two approaches was analyzed. The cut-off values for diagnosis by ARFI (S more than 2, S more than 3 and S = 4) were determined by generating a receiver operating characteristic (ROC) curve. The spectrum of liver stiffness detected by ARFI sonoelastography included S1 at (1.26+/-0.27) m/s (n = 36), S2 at (1.45+/-0.51) m/s (n = 31), S3 at (2.01+/-0.54) m/s (n = 27), and S4 at (2.28+/-0.82) m/s (n = 14). The ARFI values were significantly different among the four different stages of liver fibrosis (P less than 0.001). The liver stiffness detected by ARFI sonoelastography was significantly correlated with the liver fibrosis stage determined by the gold standard pathohistological analysis (Spearman's rank coefficient: 0.61, P less than 0.001). Using the ARFI technology for assessment of liver fibrosis gave areas under the ROC curve of 0.779 for S more than 2 patients, of 0.863 for S more than 3 patients, and of 0.0880 for S = 4 patients. The real-time ultrasound elastography ARFI technology can show the elasticity modulus of liver, and its data values positively correlate with the patho-histology grade of liver fibrosis in CHC patients. ARFI technology is easy to operate, non-invasive, and quantitative, and has potential clinical value for assessing liver fibrosis in CHC. Source

Zhou Y.,Peking University | Liu Y.,The 302 Hospital of PLA | Yang S.X.,Peking University | Wang Z.,Peking University
Genetics and Molecular Research

This study examined the effects of N-ethylmaleimide-sensitive factor (NSF) small interfering RNA (siRNA) on cardiac function following myocardial infarction (MI) in rats. Thirty-six adult Sprague Dawley rats were randomly divided into three equivalent groups. An acute MI model was established by ligating the anterior descending branch of the left coronary artery and confirmed by electrocardiogram. Recombinant NSF-siRNA adenovirus (experimental), negative adenovirus (control), and normal saline were injected near the infarcted area of the left ventricle in each respective group. The left ventricular ejection fraction (LVEF) was measured with a noninvasive ultrasonic cardiogram. Left ventricular end-diastolic pressure (LVEDP) and the maximum rate of rise in left ventricular pressure (+dp/dt max) were measured using the BL-420 Biological Functional Experimental System. Hearts were sectioned and stained with 2,3,5,-triphenyl tetrazolium chloride (TTC) to observe the MI area. Two weeks after surgery, LVEF in the experimental group (46.0 ± 7.5%) was higher than control (34.0 ± 6.0%) and saline (37.5 ± 4.5%) group LVEFs (P < 0.05), whereas LVEDP was the lowest in the experimental group (18.51 ± 6.87 vs 29.47 ± 9.94 and 26.58 ± 8.97 mmHg, respectively) (P < 0.05). The +dp/dt max was also higher in the experimental group (9.74 ± 1.16 vs 4.33 ± 1.19 and 5.24 ± 1.53 mmHg/s x 103, respectively) (P < 0.05); however, the MI area did not differ significantly between groups. Local injection of an adenovirus-mediated NSF-siRNA expression vector near infarcted areas improved cardiac function two weeks after MI, but had no impact on the MI area. © FUNPEC-RP. Source

Zhang D.,The 302 Hospital of PLA | Li P.,Peking University | Chen M.,The 302 Hospital of PLA | Liu L.,Shanxi Medical University | And 3 more authors.
Abdominal Imaging

Objectives: To investigate the diagnostic performance of the acoustic radiation force impulse (ARFI) elastography for the assessment of the liver fibrosis in alcoholic liver disease (ALD). Methods: We included 112 patients with ALD in whom liver biopsy, ARFI elastography, and aspartate-to-platelet ratio index (APRI) measurements were performed. Results: ARFI elastography correlated significantly with histological fibrosis (r = 0.685, P < 0.001) in patients with ALD. The diagnostic accuracies expressed as areas under receiver operating characteristic (AUROC) curves for ARFI elastography and APRI were 0.846 and 0.763 for the diagnosis of significant fibrosis (S ≥ 2), 0.875 and 0.688 for the diagnosis of severe fibrosis (S ≥ 3), and 0.893 and 0.648 for the diagnosis of liver cirrhosis, respectively. The AUROC values of ARFI elastography were significantly better than those of APRI for predicting severe fibrosis (P = 0.02) and cirrhosis (P = 0.04). The optimum cutoff values for ARFI elastography were 1.33 m/s for S ≥ 2, 1.40 m/s for S ≥ 3, and 1.65 m/s for S = 4 in patients with elevated alanine aminotransferase (ALT) levels; these decreased to 1.24 m/s for S ≥ 2, 1.27 m/s for S ≥ 3, and 1.41 m/s for S = 4 in patients with normal ALT. Conclusion: ARFI elastography is an acceptable method for predicting the severity of fibrosis in patients with ALD. ARFI elastography is influenced by elevated aminotransferase levels in ALD. © 2014, Springer Science+Business Media New York. Source

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