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Liu Y.,The 302 Hospital of Chinese PLA | Zhou Y.,Capital Medical University | Yang S.-X.,Capital Medical University | Wang Z.,Capital Medical University
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: How to reduce the incidence and mortality of cardiovascular diseases is an urgent concern in the field of public health. OBJECTIVE: To explore the influence of adenovirus-mediated NSF-siRNA release from vesoactive substance on the cardiac function of a rat model of myocardial infarction. METHODS: A total of 36 adult Sprague-Dawley rats were applied to establish acute myocardial infarction models by ligating the anterior descending branch of the left coronary artery. After the model was determined by electrocardiogram successfully, NSF-siRNA adenovirus (experimental group), negative adenovirus (control group) and normal saline (normal saline group) were injected near the infarct area of the left ventricle of rats respectively. After 2 weeks, the left ventricular ejection fraction (LVEF) was tested with noninvasive ultrasonic cardiogram. Meanwhile, the left ventricular end-diastolic pressure (LVEDP) and maximum pressure rising speed of left ventricular (dp/dt max) were detected by connecting the right external carotid artery place pipe to the BL-420 biological function experiment system, to evaluate the cardiac function. Subsequently, the rat heart was harvested for serial sections to observe the infarcts range. RESULTS AND CONCLUSION: After 2 weeks, the LVEF of the experimental group was increased remarkably (P < 0.05), while the LVEDP of the experimental group was decreased evidently compared with the control group and normal saline group (P < 0.05), and the dp/dt max of the experimental group was significantly increased (P <0.05). Furthermore, there were no significant differences in the infarct area among groups (P > 0.05). The local injection of adenovirus mediated NSF-siRNA expression vector in infarct part can improve the cardiac function indexes, including LEVF, LVEDP and dp/dt max 2 weeks after myocardial infarction, but it has no impact on the myocardial infarction area. Source

Yan L.,The 302 Hospital of Chinese PLA | Zhou Y.-S.,The 302 Hospital of Chinese PLA | Jing H.,The 302 Hospital of Chinese PLA | Peng L.,The 302 Hospital of Chinese PLA
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Normal stem cells and tumor stem cells have differences in cell signaling pathway of gene expression and dependence. How to find a therapeutic method of selectively killing tumor stem cells is a topic that should be studied greatly. OBJECTIVE: To isolate human liver cancer stem cells MHCC97 and to investigate the biological characteristics of them. METHODS: Tumor stem cells were selected from highly metastatic human hepatoma cell line MHCC97 by flow cytometry. CD133-CD34- MHCC97 from normal human liver stem cells and CD133+CD34+ MHCC97 from tumor stem cells were isolated. Their phenotypes, growth curve, cell cycle and multi-lineage differentiation were measured. RESULTS AND CONCLUSION: Phenotypes of CD133+CD34+ MHCC97 cancer stem cells were CD133+ and CD34+ and they had the same growth curve and cell cycle with the liver stem cells CD133-CD34- MHCC97, besides, they had the differential ability to epithelial cells and endothelial cells that proved to be stem cells. These results indicated that human cancer stem cells CD133+CD34+ MHCC97 had the specific characteristics of tumor stem cells, could differentiate into epithelial cells and endothelial cells, had the biological property of tumor stem cells, was an origin of tumor relapse and metastasis, and a target of clinical therapy. Source

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