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PubMed | Peking University and the 302 Hospital of Chinese PLA
Type: | Journal: Archives of oral biology | Year: 2016

Porphyromonas gingivalis induces nitric oxide (NO) synthesis in human umbilical vein endothelial cells (HUVECs). Peroxisome proliferator-activated receptor (PPAR) has an anti-inflammation function, and its involvement in this NO induction process requires elucidation. Here, we focused on PPAR expression in HUVECs exposed to P. gingivalis, and investigated its effects on NO synthesis.HUVECs were time-dependently stimulated by P. gingivalis W83 for 0-24h. PPAR expression was assessed at the mRNA and protein levels, and PPAR activation was measured using dual-luciferase reporter assays. NO synthesis and NO synthase (NOS) expression in response to P. gingivalis were examined in HUVECs pretreated with representative PPAR agonist (15-deoxy-12,14-prostaglandin J2 10M) or antagonist (GW9662 10M). In addition, NO synthesis and NOS expression in the P. gingivalis infected and control groups were detected.The PPAR mRNA level in HUVECs increased after exposure to P. gingivalis for 1h and its protein level increased at 2h. Luciferase-induced PPAR increased in P. gingivalis-exposed HUVECs. NO synthesis in the infected group at 4h, and in the PPAR-activated group at 8h, was higher than that in controls. Inducible NOS increased in the infected and PPAR-activated groups at 4 and 8h. The total endothelial NOS (eNOS) and phospho-eNOS levels were lower in the infected group than controls, but did not change in the PPAR-activated group.Activated PPAR induces NO generation through the NOS pathway in HUVECs exposed to P. gingivalis.

PubMed | PLA Fourth Military Medical University and The 302 Hospital of Chinese PLA
Type: Journal Article | Journal: International journal of oncology | Year: 2015

Resveratrol is a plant-derived natural compound which possesses potential anticancer properties. However, there are scarce reports on its anticancer effects in non-small cell lung cancer and its auxiliary function on the anticancer effects of cisplatin. In the present study, we investigated the effects of resveratrol on the cell viability and apoptosis in human non-small cell lung cancer H838 and H520 cell lines. It has been found that resveratrol inhibited the proliferation of H838 and H520 cells in a dose- and time-dependent manner, and apoptosis was increased in cells treated with resveratrol which was associated with the depolarization of mitochondrial membrane potential, release of cytochrome c from mitochondria to cytosol, and abnormal expression of Bcl-2 and Bax proteins. Above all, resveratrol enhanced the effects of cisplatin on inhibition of cancer cell proliferation, induction of cell apoptosis, depolarization of mitochondrial membrane potential, release of cytochrome c and regulation on expression of Bcl-2 and Bax. Results from the present study demonstrated that resveratrol exhibited its anticancer effects on non-small cell lung cancer H838 and H520 cell lines, and enhanced the antitumor effects of cisplatin by regulating the mitochondrial apoptotic pathway. These results have put forward the rationale for further basic research and preclinical investigation on the anticancer effects of resveratrol against human non-small cell lung cancer.

Liu Y.,The 302 Hospital of Chinese PLA | Zhou Y.,Capital Medical University | Yang S.-X.,Capital Medical University | Wang Z.,Capital Medical University
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: How to reduce the incidence and mortality of cardiovascular diseases is an urgent concern in the field of public health. OBJECTIVE: To explore the influence of adenovirus-mediated NSF-siRNA release from vesoactive substance on the cardiac function of a rat model of myocardial infarction. METHODS: A total of 36 adult Sprague-Dawley rats were applied to establish acute myocardial infarction models by ligating the anterior descending branch of the left coronary artery. After the model was determined by electrocardiogram successfully, NSF-siRNA adenovirus (experimental group), negative adenovirus (control group) and normal saline (normal saline group) were injected near the infarct area of the left ventricle of rats respectively. After 2 weeks, the left ventricular ejection fraction (LVEF) was tested with noninvasive ultrasonic cardiogram. Meanwhile, the left ventricular end-diastolic pressure (LVEDP) and maximum pressure rising speed of left ventricular (dp/dt max) were detected by connecting the right external carotid artery place pipe to the BL-420 biological function experiment system, to evaluate the cardiac function. Subsequently, the rat heart was harvested for serial sections to observe the infarcts range. RESULTS AND CONCLUSION: After 2 weeks, the LVEF of the experimental group was increased remarkably (P < 0.05), while the LVEDP of the experimental group was decreased evidently compared with the control group and normal saline group (P < 0.05), and the dp/dt max of the experimental group was significantly increased (P <0.05). Furthermore, there were no significant differences in the infarct area among groups (P > 0.05). The local injection of adenovirus mediated NSF-siRNA expression vector in infarct part can improve the cardiac function indexes, including LEVF, LVEDP and dp/dt max 2 weeks after myocardial infarction, but it has no impact on the myocardial infarction area.

Yan L.,The 302 Hospital of Chinese PLA | Zhou Y.-S.,The 302 Hospital of Chinese PLA | Jing H.,The 302 Hospital of Chinese PLA | Peng L.,The 302 Hospital of Chinese PLA
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Normal stem cells and tumor stem cells have differences in cell signaling pathway of gene expression and dependence. How to find a therapeutic method of selectively killing tumor stem cells is a topic that should be studied greatly. OBJECTIVE: To isolate human liver cancer stem cells MHCC97 and to investigate the biological characteristics of them. METHODS: Tumor stem cells were selected from highly metastatic human hepatoma cell line MHCC97 by flow cytometry. CD133-CD34- MHCC97 from normal human liver stem cells and CD133+CD34+ MHCC97 from tumor stem cells were isolated. Their phenotypes, growth curve, cell cycle and multi-lineage differentiation were measured. RESULTS AND CONCLUSION: Phenotypes of CD133+CD34+ MHCC97 cancer stem cells were CD133+ and CD34+ and they had the same growth curve and cell cycle with the liver stem cells CD133-CD34- MHCC97, besides, they had the differential ability to epithelial cells and endothelial cells that proved to be stem cells. These results indicated that human cancer stem cells CD133+CD34+ MHCC97 had the specific characteristics of tumor stem cells, could differentiate into epithelial cells and endothelial cells, had the biological property of tumor stem cells, was an origin of tumor relapse and metastasis, and a target of clinical therapy.

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