Yan L.,Jilin Institute of Architecture and Civil Engineering |
Han-Xiaowei,Jilin Institute of Architecture and Civil Engineering |
Wen-Yang D.,The 208th Hospital of PLA
Applied Mechanics and Materials | Year: 2013
In the framework of the actual structure of projects and inevitably there are some uncertainties. Interval finite element method can effectively function to define the scope and provide a strict mathematical sense, the results of the calculations, and finite element method for interval data can be expressed uncertainty. In this paper, the theoretical calculation of interval finite element of a layer rigid frame structure, compared the simulation results with the combination of ANSYS modeling, Numerical analysis of examples from the results of the theoretical value and the true value of the error between the results of a very small can be proved that the interval finite element theory and method for frame structures is very applicable. © (2013) Trans Tech Publications, Switzerland.
Wang Z.,Jilin University |
Wang B.,Jilin University |
Lou J.,The 208th Hospital of PLA |
Yan J.,Jilin University |
And 4 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2014
Recombinant adenovirus (Ad) vectors are widely used for both in vitro and in vivo gene transfer. However, intravenous administration of Ad vectors results mainly in hepatocyte transduction and subsequent hepatotoxicity. Coxsackie-adenovirus receptor (CAR) and αvβ integrins, which are functional receptors for the fiber and penton proteins, respectively, are the tropism determinants of Ad type 5 (Ad5). We previously developed a system for rapid construction of fiber-modified Ad5 vectors. We also constructed a fiber-modified Ad5 containing an Arg-Gly-Asp (RGD) motif in the HI-loop and showed that it could enhance anti-tumor effects in vitro and in vivo. Here, we constructed a novel Ad5 vector containing two amino acid mutations in the AB loop of the fiber-modified Ad5 fiber knob and showed that it could significantly reduce liver tropism and increase gene transfer in low-CAR or CAR-deficient cancer cells following intravascular delivery. However, anti-tumor effects of the fiber-mutated Ad5 expressing HSV-TK under control of the hTERT promoter was not found when compared with an unmodified Ad5 vector in cancer lines expressing different levels of CAR, likely due to the activity of the hTERT promoter being lower than that of the CMV promoter. Nevertheless, this study describes an enhanced Ad5 vector for intravascular gene delivery, and further modifications such as changes in the promoter may facilitate the development of this vector for cancer treatment. © 2014, Int J Clin Exp Med. All Rights Reserved.
Teng D.-K.,The 208th Hospital of PLA |
Wang H.,Jilin University |
Lin Y.-Q.,Jilin University |
Sui G.-Q.,Jilin University |
And 2 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2012
Background: To investigate the value of ultrasound elastography (UE) in the differentiation between benign and malignant enlarged cervical lymph nodes (LNs). Methods: B-mode ultrasound, power Doppler imaging and UE were examined to determine LN characteristics. Two kinds of methods, 4 scores of elastographic classification and a strain ratio (SR) were used to evaluate the ultrasound elastograms. Results: The cutoff point of SR had high utility in differential diagnosis of benign and malignant of cervical lymph nodes, with good sensitivity, specificity and accuracy. Conclusion: UE is an important aid in differential diagnosis of benign and malignant cervical LNs.
Li Y.,Jilin Institute of Architecture and Civil Engineering |
Wen-Yang D.,The 208th Hospital of PLA |
Bo W.,Jilin Institute of Architecture and Civil Engineering
Applied Mechanics and Materials | Year: 2013
Mass concrete temperature cracking is an important problem of concrete foundation safety in civil engineering projects, which exists from construction process of concrete foundation to operation period. This paper introduces the simulation method of mass concrete temperature cracking propagation process by Finite Element Method(FEM). With the concrete heat conduction problem, an example to study concrete surface cracks of the whole process of pouring concrete caused by temperature changes and discussed how concrete cracking process brought from temperature decrease is simulated by FEM. The results show that the finite element method can effectively simulate the development of concrete block cracks under temperature stress. The calculation result accord the cracks distribution law of mass concrete blocks in the uniform temperature drop and the basis of strong constraints. © (2013) Trans Tech Publications, Switzerland.
Gao H.-C.,Jilin University |
Zhu K.,The 208th Hospital of PLA |
Gao H.-M.,Jilin University |
Miao C.-S.,Jilin University |
And 3 more authors.
Experimental and Therapeutic Medicine | Year: 2015
The aim of this study was to investigate the role of tissue transglutaminase (tTG) in the pathogenesis of diabetic cardiomyopathy (DCM) and the intervention effect of rutin. DCM was induced in rats by the injection of streptozotocin (STZ; 25 mg/kg). After a preliminary examination, the rats were randomly divided into four groups: Control (n=8), STZ-induced DCM (n=8), STZ + positive drug (captopril; n=6) and STZ + rutin (n=8) groups. The DCM model was evaluated using blood sugar values, serum enzyme levels, hematoxylin and eosin staining and Masson's staining, ex vivo. The protein and mRNA expression of tTG was assessed with immunohistochemistry, western blotting and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The rat model of DCM was successfully established by STZ administration, and the expression levels of tTG were significantly increased in the DCM model. Following the injection of captopril or rutin, the blood sugar values, collagen content and expression levels of tTG were gradually reduced and serum enzyme levels were increased, as compared with those in the STZ-induced DCM group. In conclusion, tTG plays an important role in STZ-induced DCM. In addition, rutin may inhibit the expression of tTG and regulate myocardial injury in STZ-induced DCM. © 2015, Spandidos Publications. All rights reserved.