Changchun, China
Changchun, China

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Wang Z.,Jilin University | Wang B.,Jilin University | Lou J.,The 208th hospital of PLA | Yan J.,Jilin University | And 4 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2014

Recombinant adenovirus (Ad) vectors are widely used for both in vitro and in vivo gene transfer. However, intravenous administration of Ad vectors results mainly in hepatocyte transduction and subsequent hepatotoxicity. Coxsackie-adenovirus receptor (CAR) and αvβ integrins, which are functional receptors for the fiber and penton proteins, respectively, are the tropism determinants of Ad type 5 (Ad5). We previously developed a system for rapid construction of fiber-modified Ad5 vectors. We also constructed a fiber-modified Ad5 containing an Arg-Gly-Asp (RGD) motif in the HI-loop and showed that it could enhance anti-tumor effects in vitro and in vivo. Here, we constructed a novel Ad5 vector containing two amino acid mutations in the AB loop of the fiber-modified Ad5 fiber knob and showed that it could significantly reduce liver tropism and increase gene transfer in low-CAR or CAR-deficient cancer cells following intravascular delivery. However, anti-tumor effects of the fiber-mutated Ad5 expressing HSV-TK under control of the hTERT promoter was not found when compared with an unmodified Ad5 vector in cancer lines expressing different levels of CAR, likely due to the activity of the hTERT promoter being lower than that of the CMV promoter. Nevertheless, this study describes an enhanced Ad5 vector for intravascular gene delivery, and further modifications such as changes in the promoter may facilitate the development of this vector for cancer treatment. © 2014, Int J Clin Exp Med. All Rights Reserved.


Teng D.-K.,The 208th Hospital of PLA | Wang H.,Jilin University | Lin Y.-Q.,Jilin University | Sui G.-Q.,Jilin University | And 2 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2012

Background: To investigate the value of ultrasound elastography (UE) in the differentiation between benign and malignant enlarged cervical lymph nodes (LNs). Methods: B-mode ultrasound, power Doppler imaging and UE were examined to determine LN characteristics. Two kinds of methods, 4 scores of elastographic classification and a strain ratio (SR) were used to evaluate the ultrasound elastograms. Results: The cutoff point of SR had high utility in differential diagnosis of benign and malignant of cervical lymph nodes, with good sensitivity, specificity and accuracy. Conclusion: UE is an important aid in differential diagnosis of benign and malignant cervical LNs.


PubMed | Jilin University and The 208th Hospital of PLA
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2015

The aim of this study was to investigate the role of tissue transglutaminase (tTG) in the pathogenesis of diabetic cardiomyopathy (DCM) and the intervention effect of rutin. DCM was induced in rats by the injection of streptozotocin (STZ; 25 mg/kg). After a preliminary examination, the rats were randomly divided into four groups: Control (n=8), STZ-induced DCM (n=8), STZ + positive drug (captopril; n=6) and STZ + rutin (n=8) groups. The DCM model was evaluated using blood sugar values, serum enzyme levels, hematoxylin and eosin staining and Massons staining,


Yan L.,Jilin Institute of Architecture and Civil Engineering | Han-Xiaowei,Jilin Institute of Architecture and Civil Engineering | Wen-Yang D.,The 208th Hospital of PLA
Applied Mechanics and Materials | Year: 2013

In the framework of the actual structure of projects and inevitably there are some uncertainties. Interval finite element method can effectively function to define the scope and provide a strict mathematical sense, the results of the calculations, and finite element method for interval data can be expressed uncertainty. In this paper, the theoretical calculation of interval finite element of a layer rigid frame structure, compared the simulation results with the combination of ANSYS modeling, Numerical analysis of examples from the results of the theoretical value and the true value of the error between the results of a very small can be proved that the interval finite element theory and method for frame structures is very applicable. © (2013) Trans Tech Publications, Switzerland.


Hou Z.-H.,The 208th Hospital of PLA | Zhao W.-C.,The 208th Hospital of PLA | Zhang Q.,The 208th Hospital of PLA | Zheng W.,The 208th Hospital of PLA
Asian Pacific Journal of Cancer Prevention | Year: 2015

Objective: To explore effects of paclitaxel-loaded poly lactic-co-glycolic acid (PLGA) particles on the viability of human hepatocellular carcinoma (HCC) HepG2 cells. Materials and Methods: The viability of HepG2 cells was assessed using MTT under different concentrations of prepared paclitaxel-loaded particles and paclitaxel (6.25, 12.5, 25, 50, and 100 mg/L), and apoptosis was analyzed using Hochest33342/Annexin V-FITC/PI combined with an IN Cell Analyzer 2000. Results: Paxlitaxel-loaded nanoparticles were characterized by narrow particle size distribution (158.6 nm average particle size). The survival rate of HepG2 cells exposed to paclitaxel-loaded PLGA particles decreased with the increase of concentration and time period (P<0.01 or P<0.05), the dose- and time-dependence indicating sustained release (P<0.05). Moreover, apoptosis of HepG2 cells was induced, again with an obvious dose- and time-effect relationship (P<0.05). Conclusions: Paclitaxel-loaded PLGA particles can inhibit the proliferation and induce the apoptosis of HCC HepG2 cells. This new-type of paclitaxel carrier body is easily made and has low cost, good nanoparticle characterization and sustained release. Hence, paclitaxel-loaded PLGA particles deserve to be widely popularized in the clinic.


Tang W.,the 208th Hospital of PLA | Wang Z.,the 208th Hospital of PLA | Liu Y.,the 208th Hospital of PLA | Zhu J.,the 208th Hospital of PLA
Cell Biochemistry and Biophysics | Year: 2015

Acute respiratory distress syndrome is a life threatening respiratory condition characterized by breakdown of the alveolar-capillary barrier, leading to flooding of the alveolar space producing the classical chest radiograph of bilateral pulmonary infiltrates. In this study, we employed lung protective ventilation strategies in patients without acute lung injury (ALI) to determine whether mechanical ventilation with lower tidal volume would provide more clinical benefits to patients without ALI. © 2014, Springer Science+Business Media New York.


Li Y.,Jilin Institute of Architecture and Civil Engineering | Wen-Yang D.,The 208th Hospital of PLA | Bo W.,Jilin Institute of Architecture and Civil Engineering
Applied Mechanics and Materials | Year: 2013

Mass concrete temperature cracking is an important problem of concrete foundation safety in civil engineering projects, which exists from construction process of concrete foundation to operation period. This paper introduces the simulation method of mass concrete temperature cracking propagation process by Finite Element Method(FEM). With the concrete heat conduction problem, an example to study concrete surface cracks of the whole process of pouring concrete caused by temperature changes and discussed how concrete cracking process brought from temperature decrease is simulated by FEM. The results show that the finite element method can effectively simulate the development of concrete block cracks under temperature stress. The calculation result accord the cracks distribution law of mass concrete blocks in the uniform temperature drop and the basis of strong constraints. © (2013) Trans Tech Publications, Switzerland.


PubMed | the 208th Hospital of PLA
Type: Journal Article | Journal: Chinese journal of cancer research = Chung-kuo yen cheng yen chiu | Year: 2013

Extraskeletal osteosarcoma (EOS) is rare and commonly arises in the retroperitoneum, limbs, head and neck. There is no significant difference between EOS and other malignant tumors in soft tissue. Localized pain and swelling are the common presenting symptoms. Clinical diagnosis of EOS is difficult, imaging techniques may be helpful and careful, and the histopathological analysis is necessary. The common histological variants of EOS include: osteoblastoma, chondroblastoma, and fibroblastoma, and other unusual subtypes were reported occasionally. It should be distinguished with myositis ossificans, malignant mesenchymoma, giant cell tumor and parosteal osteosarcoma. We present an EOS arising in the penis. The primary site and histological category of the tumor were extremely rare. We hope the case will be helpful to the recognition of clinical signs, iconography and histopathology of EOS.


PubMed | The 208th Hospital of PLA
Type: Journal Article | Journal: Asian Pacific journal of cancer prevention : APJCP | Year: 2015

To explore effects of paclitaxel-loaded poly lactic-co-glycolic acid (PLGA) particles on the viability of human hepatocellular carcinoma (HCC) HepG2 cells.The viability of HepG2 cells was assessed using MTT under different concentrations of prepared paclitaxel-loaded particles and paclitaxel (6.25, 12.5, 25, 50, and 100 mg/L), and apoptosis was analyzed using Hochest33342/Annexin V-FITC/PI combined with an IN Cell Analyzer 2000.Paxlitaxel-loaded nanoparticles were characterized by narrow particle size distribution (158.6 nm average particle size). The survival rate of HepG2 cells exposed to paclitaxel-loaded PLGA particles decreased with the increase of concentration and time period (P<0.01 or P<0.05), the dose- and time-dependence indicating sustained release (P<0.05). Moreover, apoptosis of HepG2 cells was induced, again with an obvious dose- and time-effect relationship (P<0.05).Paclitaxel-loaded PLGA particles can inhibit the proliferation and induce the apoptosis of HCC HepG2 cells. This new-type of paclitaxel carrier body is easily made and has low cost, good nanoparticle characterization and sustained release. Hence, paclitaxel- loaded PLGA particles deserve to be widely popularized in the clinic.


PubMed | the 208th Hospital of PLA
Type: Journal Article | Journal: Cell biochemistry and biophysics | Year: 2016

Acute respiratory distress syndrome is a life threatening respiratory condition characterized by breakdown of the alveolar-capillary barrier, leading to flooding of the alveolar space producing the classical chest radiograph of bilateral pulmonary infiltrates. In this study, we employed lung protective ventilation strategies in patients without acute lung injury (ALI) to determine whether mechanical ventilation with lower tidal volume would provide more clinical benefits to patients without ALI.

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