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Zheng Y.-K.,The 184 Hospital of Chinese PLA | Zhang C.-Q.,The First Affiliated Hospital of Kunming Medical College | Tang Y.-F.,The 184 Hospital of Chinese PLA | Tang S.-H.,The 184 Hospital of Chinese PLA | And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: It is not clear whether the Cathepsis family is involved in the pathological process of early spinal cord injury and whether high-dose methylprednisolone plays neuroprotective effect through lysosome apoptosis pathway. OBJECTIVE: To explore the expression and change of genes Cathepsin family in early spinal cord injury and to identify if high-dose methylprednisolone plays neuroprotective effect by the lysosome apoptosis pathway. METHODS: Nine Japanese rabbits were randomly divided into three groups, the rabbits in the model group and drug treatment group were treated with laminectomy, and then the rabbits were used to establish the acute spinal cord injury model with Allen falling strike method. The rabbits in the drug treatment group were treated with human equivalent dose flushing-dose methylprednisolone at 2 hours after acute spinal cord injury. The rabbits in the control group were treated with laminectomy. All rabbits were killed at 8 hours after acute spinal cord injury, and then the damaged spinal cord tissues were obtained carefully. Total RNA was extracted from above nine samples with Trizol One-step method to undergo the examination of the gene expression profile by using Agilent Rabbit Oligo Microarray (4×44K) respectively. GeneSpring 11.0 software was then used to filter potential candidate genes, and only genes with P values ≤0.01 and fold change≥2 were retained for further analysis. RESULTS AND CONCLUSION: The spinal cord injury models were successfully set up and the corresponding tissue specimens were obtained. Acquired nine subsample of total RNA were qualified for microarray examination. The results of microarray examination showed that among the 10 genes of Cathepsin family, only Cathepsin Z and Procathepsin E showed significant different expression. All of Cathepsin family genes of Cathepsin C, D, F, K, L, S and W did not showed significant different expression. There were no significant differences of Cathepsin family genes expressions between drug treatment group and model group after treated with methylprednisolone. Gene Cathepsin Z and Procathepsin E took part in the apoptosis of spinal cord injury at acute phase, but high-dose methylprednisolone cannot play neuroprotective effect by the lysosome apoptosis pathway.


Zheng Y.-K.,The 184 Hospital of Chinese PLA | Li H.-K.,Kunming Medical University | Tang S.-H.,The 184 Hospital of Chinese PLA | Zhao M.,The 184 Hospital of Chinese PLA | And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: The diagnosis of deep vein thrombosis is mainly dependent on imaging and laboratory tests, and the most fatal lack is that thrombosis can be confirmed until it has been formed. Accordingly, it is necessary to explore inherent rules using molecular technology. OBJECTIVE: To observe the Hmox-1 expression in the process of formation of traumatic deep vein thrombosis in rats and to discuss the feasibility of Hmox-1 as a molecular marker for predictive diagnosis of deep vein thrombosis. METHODS: Bilateral femoral veins were clamped and both hind limbs were fixed in rats to prepare traumatic deep venous thrombosis models. According the modeling time and formation of thrombosis, 100 experimental rats were randomized into five groups: normal group, trauma group, pre-thrombosis group, group of thrombosis formation at peak stage, group of non-thrombosis formation at peak stage. Real time-PCR was used to detect blood Hmox-1 mRNA expression. Bilateral femoral veins were isolated for histological observation of thrombosis degree. RESULTS AND CONCLUSION: At 25 hours after modeling, the thrombosis rate was 58.46% (38/65). mRNA expression of Hmox-1 in the pre-thrombosis group and group of thrombosis formation at peak stage was higher than that of the normal group (P < 0.05). There was no significant difference in the Hmox-1 mRNA expression between the normal group and the group of non-thrombosis formation at peak stage. These findings indicate that the variation tendency of Hmox-1 in the blood is consistent with the biological process of thrombosis formation, and Hmox-1 may become a marker for predictive diagnosis of deep venous thrombosis.

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