The 180th Hospital of PLA
The 180th Hospital of PLA
Deng C.,Fujian Medical University |
Xiong J.,Fujian Medical University |
Gu X.,Fujian Medical University |
Chen X.,Fujian Medical University |
And 5 more authors.
Oncotarget | Year: 2017
Epidermal growth factor receptor (EGFR) overexpression is related to the increased aggressiveness, metastases, and poor prognosis in various cancers. In this study, we successfully constructed a new EGFR nanobody-based immunotoxin rE/CUS containing cucurmosin (CUS), The immunotoxin was expressed by prokaryotic system and we obtained a yield of 5 mg protein per liter expression medium. The percentage of it's binding ability totumor cell lines A549, HepG2, SW116, which highly expressed EGFR was 55.6%, 79.6% and 97.1%, respectively, but SW620 was only 4.45%. rE/ CUS has the ability to bind A549, HepG2, SW116 cells specifically, and the antigen binding capability was not affected because of extra part of CUS component. The rE/ CUS significantly inhibited the cell viability against EGFR over expression tumor cell lines in a dose-and time-dependent manner. Moreover, rE/CUS also induced apoptosis of HepG2 and A549 mightily. Our results demonstrate that rE/CUS is a potential therapeutic strategy for treating EGFR-positive solid tumors. © Deng et al.
PubMed | The 180th Hospital of PLA and University of Pennsylvania
Type: | Journal: Journal of anesthesia | Year: 2016
The emergency difficult airway with the cannot intubate and cannot ventilate (CICV) situation contributes to a high percentage of anesthesia- and emergency medicine-related morbidity and mortality. A new technique of supraglottic jet oxygenation and ventilation (SJOV) via the nasal approach was successfully used in an emergency to save a patient with a CICV difficult airway from a catastrophic outcome.
Wu X.-Y.,The 180th Hospital of PLA |
Jiang L.-Z.,Guangxi Medical University
International Eye Science | Year: 2015
AIM: To evaluate the effects of intracameral cefuroxime injection on the prophylaxis of postoperative endophthalmitis after cataract surgeries, and compare endophthalmitis conditions between intracameral cefuroxime injection group and no intervention control group, using Meta analysis to search literatures at home and abroad. METHODS: By searching through Pubmed, the Cochrane Library, EMBASE, QVIP, CNKI, Wanfang, the literatures published from January 1st 2006 to January 31rd 2015 were collected. The data in five literatures about the effects of intracameral cefuroxime injection on the prophylaxis of endophthalmitis after cataract surgeries were analyzed. By extracting data, incidence of endophthalmitis after cataract surgeries with taking the preventive measures or not taking was compared by Review Manager 5.2.Adopting fixed effect model, values of odds ratio (OR) and 95% Confidence Interval (95%CI) were recorded and compared, analyzed sensitivity analysis, excluded heterogeneity and calculated outcomes. RESULTS: After comparing the incidence of endophthalmitis from intracameral cefuroxime injection group and control group, the result was statistically significant [OR=0.11 (P<0.01); 95% CI: 0.07~0.18]. CONCLUSION: Intracameral cefuroxime injection can effectively decrease the occurrence of endophthalmitis after cataract surgeries. Copyright 2015 by the IJO Press.
PubMed | The 180th Hospital of PLA and Southern Medical University
Type: | Journal: Medical science monitor : international medical journal of experimental and clinical research | Year: 2016
BACKGROUND Thrombolysis with rtPA is the only accepted drug therapy for acute ischemic stroke. Since acute cerebral stroke is so pervasive, newly developed recanalization methods have the potential for wide-ranging impacts on patient health and safety. We explored the efficacy and safety of Solitaire stent arterial embolectomy in the treatment of acute cardiogenic cerebral embolism. MATERIAL AND METHODS Between October 2012 and June 2015, 17 patients underwent Solitaire stent arterial embolectomy, either alone or in combination with rtPA intravenous thrombolysis, to treat acute cardiogenic cerebral embolism. Sheath placement time, vascular recanalization time, number of embolectomy attempts, and IV rtPA dose and time were recorded. Success and safety of the recanalization procedure, as well as clinical outcomes, were assessed. These results were compared to 16 control patients who were treated using only rtPA IV thrombolysis. RESULTS Full recanalization of the occluded arteries was achieved in 15 (88.2%) of the Solitaire stent patients. NIH Stroke Scale scores of embolectomy patients improved by an average of 12.59 8.24 points between admission and discharge, compared to 5.56 5.96 in the control group (P<0.05). Glasgow Coma Score improvement between admission and discharge was also significantly higher in the embolectomy group (P<0.05). There was no significant difference in symptomatic intracerebral hemorrhage, high perfusion encephalopathy, incidence of hernia, or mortality between the 2 groups (P>0.05). CONCLUSIONS Solitaire stent embolectomy is a safe and effective alternative to simple venous thrombolytic therapy, and it can significantly improve short-term neurological function and long-term prognosis in acute cardiogenic cerebral embolism.
Fan J.-H.,the 180th Hospital of PLA |
Wang M.-H.,the 180th Hospital of PLA
International Eye Science | Year: 2016
AIM: To explore the effect of comprehensive therapy in the treatment of fungal corneal ulcer and clinical outcome. METHODS: A total of 159 cases 159 eyes fungal corneal ulcer patients took comprehensive therapy of 10 measures, followed up more than 3mo to observe the treatment effect and the final visual acuity in our hospital for treatment. RESULTS: The average hospitalization time of fungal corneal ulcer was (12.11±5.13)d. A total of 148 cases of 148 eyes were cured, and the cure rate was 93.1%; improvement in 8 cases, accounted for 5.0%; 3 cases were ineffective, accounted for 1.9%. The final visual acuity of 89 eyes ≥0.3, accounted for 56.0% (89/159). The final visual acuity of 136 eyes ≥0.1, accounting for 85.5% (136/159), and accounting for 91.9% in 148 cured patients. CONCLUSION: The comprehensive therapy in the treatment of fungal corneal ulcer has higher cure rate and visual acuity. Copyright 2016 by the IJO Press.
Zhong F.-L.,the 180th Hospital of PLA |
Li X.-X.,the 180th Hospital of PLA |
Wang C.-L.,the 180th Hospital of PLA
International Eye Science | Year: 2016
AIM: To discuss the application of anterior segment optic coherence tomography (AS-OCT) in the diagnosis of corneal ulcer. METHODS: The cross linear scan was used in 88 patients (88 eyes) with corneal lesion by AS-OCT to gather the image data, observe the pathological changed tissue by measuring all layers for patients with initial inspection, providing important visual images and data for treatments. All the patients were followed up for 2 mo. RESULTS: Clear images with structure of all layers were obtained. It can provide the intuitive image data and scan the same position and show the changes during the treatment. CONCLUSION: AS-OCT can discover the important condition immediately. It also can monitor course of disease dynamically, provide the intuitive image data for clinical treatment. Copyright 2016 by the IJO Press.
Fu M.,The 180th Hospital of PLA
American Journal of Therapeutics | Year: 2015
Statins have been used to prevent contrast-induced nephropathy (CIN). However, the optimal dose of statins is still under controversy. This study aimed to investigate the optimal dose of atorvastatin for the treatment of CIN after carotid artery stenting (CAS). Seventy-six patients receiving selective CAS were randomized to receive 3 different dose of atorvastatin (low dose, 20 mg, n = 30; intermediate dose, 40 mg, n = 24; high dose, 60 mg, n = 22). Preoperatively and on day 3 postoperatively, the levels of serum creatinine, blood urea nitrogen, high-sensitivity C-reactive protein (hs-CRP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and creatine kinase (CK) were measured. Creatinine clearance (Ccr) and CIN incidence were calculated. In patients treated with high-dose atorvastatin, no significant change was observed in levels of serum creatinine (Scr), blood urea nitrogen (BUN), creatinine clearance, and high-sensitivity C-reactive protein after the CAS procedure (P > 0.05). The CIN incidence in the high-dose group (0%) was significantly lower than the low-dose (13.3%) and intermediate (8.3%) groups (P < 0.05). In the high-dose group, levels of alanine aminotransferase, aspartate aminotransferase, and creatine kinase were significantly increased after CAS (P < 0.05). Pretreatment with 40 mg of atorvastatin is both effective and safe in preventing CIN after CAS. Adverse events of the live and heart should be closely monitored during atorvastatin treatment. Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.
Li W.,The 180th Hospital of PLA |
Wei R.,The 180th Hospital of PLA
Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology | Year: 2014
Background: Transscleral diode laser cyclophotocoagulation (TDCP) was used to later stage of glaucoma in the past. However, the indications of TDCP are expanding gradually over time and there are more and more reports about its application in different stages and different types of glaucoma. Objective: This study was to compare the clinical efficacy of TDCP and trabeculectomy for primary acute angle-closure glaucoma (PAACG) with persistent ocular hypertension. Methods: Ninety-five PAACG patients with persistent ocular hypertension were retrospectively analyzed. The patients were divided into TDCP group (62 eyes of 56 cases) and trabeculectomy group (44 eyes of 39 cases) based on the difference of treating fashions. The clinical outcomes were compared between the two groups with the evaluating indexes of visual acuity, intraocular pressure (IOP) before and 1 week, 3 months and the end of following-up as well as the number of drug used and the re-treatment. Results: The demography was matched between the two groups (all at P≤0.05). The number of eyes with visual improvement was significantly different among the groups (χ2=30.927, P=0.002) in the TDCP group. Compared with before treatment, the number of visual improvement in 1 week after treatment was significantly raised (χ2=16.778, P=0.002). No significant differences were seen in the eye number of visual improvement in various timepoints after treatment (all at P≤0.008). Also, the eye number of visual improvement was significantly different among the different timepoints (χ2=44.345, P<0.001), and statistically significant change was found between the preoperation and postoperative 1week (χ2=23.725, P<0.001). However, there were no significant differences in the eye number of visual improvement in various timepoints after operation each other (all at P≤0.008). The IOP was insignificantly altered among the groups at various timepoints (F=3.836, P=0.053), but the change was significantly different over time (Ftime=757.078, P<0.001). The postoperative IOP was significantly higher than that of preoperation in both groups (all at P<0.001). The proportion of eyes using lowering-IOP drugs was 48.4% in the TDCP group, and that in the trabeculectomy group was 27.3%, showing a significant difference between them (χ2=4.796, P=0.029). The re-therapy was performed in 20 eyes (32.3%) and 2 eyes (4.5%) in the TDCP group and the trabeculectomy group, respectively. Conclusions: The short-term lowing-IOP effecacy of TDCP is similar to trabeculectomy, but the long-term lowing-IOP efficacy of TDCP is not good. Compared with the trabeculectomy, TDCP shows more simple operation, less time-consuming during the surgery and milder tissue damage, but the eyes continuing to use anti-glaucomatous drugs or re-therapy are increased. Copyright © 2014 by the Chinese Medical Association.
Xu J.-F.,The 180th Hospital of PLA |
Chen K.-C.,The 180th Hospital of PLA
International Eye Science | Year: 2014
AIM: To investigate the efficiency and security of compound anisodine injection in the treatment of juxtafoveal central serous chorioretinopathy (CSC). METHODS: Sixty patients (60 eyes) who were diagnosed of juxtafoveal CSC were assigned randomly into 2 groups: 32 cases (32 eyes, therapeutic group) were injected subcutaneously compound anisodine injection for 2 mL q. d around superficial temporal arteries in the affected eyes; 28 cases (28 eyes, control group) received only traditional oral medication. Both groups received therapy for 2 to 4 courses of treatment. The main observations were the best corrected visual acuity (BCVA), subjective symptom, visual field, average light sensitivity and optical coherent topography (OCT). RESULTS: There was no significant difference between the therapeutic group and the control group before treatment (P>0.05), but all the outcome measures at 1, 3mo in the treatment group were significantly improved as compared with control group (P<0.05). After 6 mo, there were no significant difference between the two groups in all measures (P>0.05). No severe adverse reaction was noted except mild ones such as temporary dry mouth, dizziness and palpitation in a few cases. CONCLUSION: Compound anisodine injection has remarkable effects in the treatment of juxtafoveal CSC. It can shorten the course, improved the visual function and decreased the recurrence rate of CSC.
PubMed | The 180th Hospital of PLA
Type: | Journal: BioMed research international | Year: 2016
In our retrospective study we evaluated the efficacy of an improved amniotic membrane (AM) roll-in filling technique (AMR) combined with multilayer amniotic membrane cover to treat corneal perforation and included 46 cornea perforations 3mm in diameter treated with AMR and 20% C3F8 mixed gas filling of the anterior chamber. Anterior chamber depth, aqueous leakage, bubble maintenance time, and cornea morphology were monitored after each operation. The mean diameter of corneal perforation was 1.60 0.55mm (range 0.5-3) and the success rate of the AMR method for corneal perforation reconstruction was 100% after a single operation. Anterior chamber depth was normally reconstructed without AMR break-off, aqueous leak, or other complications. The mean time of the C3F8 gas bubble in the anterior chamber was 8.6 2.0 days (range 4-12). At the last follow-up, all patients visual acuity was improved to varying degrees. The mean follow-up time was 11.0 5.6 months (range 3-36). The AMR plugging combined with multilayer AM cover is a secure and easy intervention, which led to 100% success in our study. Various perforations ranging from trauma to infection can be treated with AMR, which is especially practical in those countries where donor cornea availability is limited.